BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0067.p (466 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIB7.1 B3KIB7_9BACI Heat shock protein HslVU, ATPase subunit HslU 696 0.0 B1DLW2.1 B1DLW2_9BACL Heat shock protein HslVU, ATPase subunit HslU 625 e-177 C0ZFA6.1 C0ZFA6_BREBN ATP-dependent hsl protease ATP-binding sub... 583 e-164 Q2B7E7.1 Q2B7E7_9BACI ATP-dependent protease ATP-binding subunit 574 e-162 Q9KA27.1 HSLU_BACHD ATP-dependent hsl protease ATP-binding subun... 574 e-162 >B3KIB7.1 B3KIB7_9BACI Heat shock protein HslVU, ATPase subunit HslU Length = 468 Score = 696 bits (1796), Expect = 0.0, Method: Compositional matrix adjust. Identities = 349/468 (74%), Positives = 386/468 (82%), Gaps = 2/468 (0%) Query: 1 MVNQSLTPRQIVAELDKYIVGQKQAKKSVAVAXXXXXXXXXXXXXXXDEVVPKNILMIGP 60 M +S+TPRQIV+ELDKYIVGQK AKKSVAVA DE+VPKNILMIGP Sbjct: 1 MSKESMTPRQIVSELDKYIVGQKDAKKSVAVALRNRYRRSLLTDDERDEIVPKNILMIGP 60 Query: 61 TGVGKTEIARRLAKLVNAPFIKVEATKFTEVGYVGRDVESMVRDLVETSIRMVKLERTEK 120 TGVGKTEIARRLAKLV+APF+KVEATKFTEVGYVGRDVESMVRDLVET+IRMVKLERTEK Sbjct: 61 TGVGKTEIARRLAKLVHAPFVKVEATKFTEVGYVGRDVESMVRDLVETAIRMVKLERTEK 120 Query: 121 VKDRAEELANERIVSILVPGGSKNKSQRNPFEMIFX--XXXXXXXXXXXXXXXXSLSERR 178 VKD+AEE+ANERIV +L P KSQRNPFEM+F SL E+R Sbjct: 121 VKDKAEEMANERIVQLLAPSTKGQKSQRNPFEMLFGGGNNETQADEEDADDKDVSLKEKR 180 Query: 179 RGVKFKLLAGQLXXXXXXXXXXXTAPTMLDMFAGQGNDQMGMNMQEMFGSXXXXXXXXXX 238 R V+F LL+G+L AP M DMFAGQGNDQMGMNMQEMFG+ Sbjct: 181 RQVRFNLLSGKLEDETIEIDVEDAAPNMFDMFAGQGNDQMGMNMQEMFGNLMPKRTKRRK 240 Query: 239 XXIREARKVLIQDEAAKLIDMDDVIQESVARAEQSGIIFIDEIDKVASQGKGSGPDVSRE 298 ++EARKVL Q+EA KLIDMDDVIQES+ RAEQSGIIFIDEIDKVASQG+ SGPDVSRE Sbjct: 241 LSVKEARKVLTQEEAGKLIDMDDVIQESIRRAEQSGIIFIDEIDKVASQGRQSGPDVSRE 300 Query: 299 GVQRDILPIVEGSTVMTKYGPVKTDYVLFMAAGAFHVAKPSDLIPELQGRFPIRVELSSL 358 GVQRDILPIVEGST+MTKYGPVKTD+VLF+AAGAFH+AKPSDLIPELQGRFPIRVEL+SL Sbjct: 301 GVQRDILPIVEGSTIMTKYGPVKTDFVLFIAAGAFHIAKPSDLIPELQGRFPIRVELNSL 360 Query: 359 TLEDFVSILTEPENALTKQYVNLLQTENIEVQFQKEAIYEIARIAASVNQNMENIGARRL 418 +L+DFVSILTEP+NALTKQYVNLL+TE++E++F EAI EIA+IAASVNQN ENIGARRL Sbjct: 361 SLDDFVSILTEPKNALTKQYVNLLRTEDLEIEFSPEAIREIAQIAASVNQNTENIGARRL 420 Query: 419 HTILEKLLEDLSFEAPELTLETMVITPEYVREKLAGIAQDRDLSQYIL 466 HTILEKLLEDLSFEAPELTL+ MVITP+YVREKLAGIAQDRDLSQYIL Sbjct: 421 HTILEKLLEDLSFEAPELTLDRMVITPQYVREKLAGIAQDRDLSQYIL 468 >B1DLW2.1 B1DLW2_9BACL Heat shock protein HslVU, ATPase subunit HslU Length = 467 Score = 625 bits (1611), Expect = e-177, Method: Compositional matrix adjust. Identities = 324/467 (69%), Positives = 368/467 (78%), Gaps = 1/467 (0%) Query: 1 MVNQSLTPRQIVAELDKYIVGQKQAKKSVAVAXXXXXXXXXXXXXXXDEVVPKNILMIGP 60 MVNQ+ TPRQIVAELDKYIVGQK AK+SVA+A DE+VPKNILMIGP Sbjct: 1 MVNQAFTPRQIVAELDKYIVGQKPAKRSVAIALRNRYRRSMLDETLRDEIVPKNILMIGP 60 Query: 61 TGVGKTEIARRLAKLVNAPFIKVEATKFTEVGYVGRDVESMVRDLVETSIRMVKLERTEK 120 TGVGKTEIARRLAKLV APFIKVEATKFTEVGYVGRDVESMVRDLVET+IRMVK E+TE Sbjct: 61 TGVGKTEIARRLAKLVKAPFIKVEATKFTEVGYVGRDVESMVRDLVETAIRMVKAEKTEA 120 Query: 121 VKDRAEELANERIVSILVPGGSKNKSQRNPFEMIFXXXXXXXXXXXXXXXXXSLSERRRG 180 V+D+AE+LANER+VS+LVP K K +NPFEM+F + +R Sbjct: 121 VQDKAEKLANERLVSLLVPSKVKEKQSKNPFEMLFGNQQNNKETVEEPEQDNVVISKRSQ 180 Query: 181 VKFKLLAGQLXXXXXXXXXXXTAPTMLDMFAGQGNDQMG-MNMQEMFGSXXXXXXXXXXX 239 + L AG+L +PTMLDM GQG D +G MNMQ+M G Sbjct: 181 ARDDLAAGKLENEIVEVEIEDNSPTMLDMLGGQGPDGLGGMNMQDMLGQFMPKKTKQRKL 240 Query: 240 XIREARKVLIQDEAAKLIDMDDVIQESVARAEQSGIIFIDEIDKVASQGKGSGPDVSREG 299 ++EARKVLIQ+EA KLI+MDDVI ESV+RAEQ+GIIFIDEIDK+AS +G+GPDVSREG Sbjct: 241 AVKEARKVLIQEEANKLINMDDVIAESVSRAEQTGIIFIDEIDKIASPSRGNGPDVSREG 300 Query: 300 VQRDILPIVEGSTVMTKYGPVKTDYVLFMAAGAFHVAKPSDLIPELQGRFPIRVELSSLT 359 VQRDILPIVEGSTV+TKYGPVKTDYVLF+AAGAFHVAKPSDLIPELQGRFPIRVEL+SL+ Sbjct: 301 VQRDILPIVEGSTVVTKYGPVKTDYVLFIAAGAFHVAKPSDLIPELQGRFPIRVELTSLS 360 Query: 360 LEDFVSILTEPENALTKQYVNLLQTENIEVQFQKEAIYEIARIAASVNQNMENIGARRLH 419 LEDFVSILTEP+NALTKQY LLQTE I ++F EAI EIA IAA +N+N ENIGARRLH Sbjct: 361 LEDFVSILTEPKNALTKQYAALLQTEGIAIEFSDEAIKEIASIAADLNRNTENIGARRLH 420 Query: 420 TILEKLLEDLSFEAPELTLETMVITPEYVREKLAGIAQDRDLSQYIL 466 TILEKLLEDLSFEAP++TL+ MVITPEYVREK+ GIA++RDLSQYIL Sbjct: 421 TILEKLLEDLSFEAPDITLDHMVITPEYVREKVGGIAKNRDLSQYIL 467 >C0ZFA6.1 C0ZFA6_BREBN ATP-dependent hsl protease ATP-binding subunit hslU Length = 473 Score = 583 bits (1502), Expect = e-164, Method: Compositional matrix adjust. Identities = 300/463 (64%), Positives = 349/463 (75%), Gaps = 2/463 (0%) Query: 4 QSLTPRQIVAELDKYIVGQKQAKKSVAVAXXXXXXXXXXXXXXXDEVVPKNILMIGPTGV 63 + LTPR IV LDKYIVGQ QAKK++AVA DEVVPKNILMIGPTGV Sbjct: 13 EQLTPRTIVEHLDKYIVGQAQAKKAIAVALRNRYRRSRLPEQMIDEVVPKNILMIGPTGV 72 Query: 64 GKTEIARRLAKLVNAPFIKVEATKFTEVGYVGRDVESMVRDLVETSIRMVKLERTEKVKD 123 GKTEIARR+AKL APFIKVEATKFTEVGYVGRDVESMVRDLVE SIR VK E+ E VK+ Sbjct: 73 GKTEIARRIAKLTGAPFIKVEATKFTEVGYVGRDVESMVRDLVEASIRTVKQEKVESVKE 132 Query: 124 RAEELANERIVSILVPGGSKNKSQRNPFEMIFXXXXXXXXXXXXXXXXXSLSERRRGVKF 183 +AE+LANE IV++LVP ++ S +NP EM F S+ ++RR V + Sbjct: 133 KAEKLANEAIVNVLVPSRKQSNSFKNPLEMFFGGQQQQQQDTTDQEEV-SIEQQRRQVMW 191 Query: 184 KLLAGQLXXXXXXXXXXXTAPTMLDMFAGQGNDQMGMNMQEMFGSXXXXXXXXXXXXIRE 243 +L GQL +P+M DMF G +QMGM MQ+M GS I++ Sbjct: 192 QLTNGQLEEQMIEIEVEDQSPSMFDMFQVPGTEQMGMQMQDMLGSLMPKRMKKRKLRIKD 251 Query: 244 ARKVLIQDEAAKLIDMDDVIQESVARAEQSGIIFIDEIDKVASQGKGSGPDVSREGVQRD 303 ARKVLIQ EA KL+DMD+V QES+ RAEQ GIIFIDEIDK+A + G GPDVSREGVQRD Sbjct: 252 ARKVLIQQEAQKLVDMDEVTQESIRRAEQHGIIFIDEIDKIAGKD-GRGPDVSREGVQRD 310 Query: 304 ILPIVEGSTVMTKYGPVKTDYVLFMAAGAFHVAKPSDLIPELQGRFPIRVELSSLTLEDF 363 ILPIVEGSTVMTKYGPVKTDYVLF+AAGAFH+AKPSDLIPELQGRFPIRVEL+SL +EDF Sbjct: 311 ILPIVEGSTVMTKYGPVKTDYVLFIAAGAFHMAKPSDLIPELQGRFPIRVELTSLRVEDF 370 Query: 364 VSILTEPENALTKQYVNLLQTENIEVQFQKEAIYEIARIAASVNQNMENIGARRLHTILE 423 V ILTEP+NAL KQYV LL+TE ++V+F EAI E+AR+AA VNQ+ +NIGARRLHTILE Sbjct: 371 VRILTEPKNALLKQYVALLETEGVQVEFTPEAIQELARLAAEVNQSTDNIGARRLHTILE 430 Query: 424 KLLEDLSFEAPELTLETMVITPEYVREKLAGIAQDRDLSQYIL 466 KLLEDLSFEAPE+ LE + ITP+YV++KL I ++DLSQYIL Sbjct: 431 KLLEDLSFEAPEIHLEVVQITPDYVKQKLGTIVGNKDLSQYIL 473 >Q2B7E7.1 Q2B7E7_9BACI ATP-dependent protease ATP-binding subunit Length = 468 Score = 574 bits (1480), Expect = e-162, Method: Compositional matrix adjust. Identities = 291/466 (62%), Positives = 345/466 (74%), Gaps = 1/466 (0%) Query: 2 VNQSLTPRQIVAELDKYIVGQKQAKKSVAVAXXXXXXXXXXXXXXXDEVVPKNILMIGPT 61 ++ +LTPRQIV LD+YI+GQK AKK+VAVA DE+ PKNILMIGPT Sbjct: 3 LSTNLTPRQIVERLDQYIIGQKDAKKAVAVALRNRYRRGLLEEAIRDEISPKNILMIGPT 62 Query: 62 GVGKTEIARRLAKLVNAPFIKVEATKFTEVGYVGRDVESMVRDLVETSIRMVKLERTEKV 121 GVGKTEIARR+AKLV APF+KVEATKFTEVGYVGRDVESMVRDLVETS+R+VK E+ + V Sbjct: 63 GVGKTEIARRMAKLVGAPFVKVEATKFTEVGYVGRDVESMVRDLVETSVRLVKEEKMQSV 122 Query: 122 KDRAEELANERIVSILVPGGSKNKSQRNPFEMIFXXXXXXXXXXXXXXXXXSLSERRRGV 181 K+RAEE AN RIV +LVP G K S +NP EM+F SL+E+R+ Sbjct: 123 KERAEENANRRIVDMLVPSGKKAASYKNPLEMLFGGGGGQQDQENEPAEDYSLTEKRKIA 182 Query: 182 KFKLLAGQLXXXXXXXXXXXTAPTMLDMFAGQGNDQMGMNMQEMFGSXXXXXXXXXXXXI 241 + KL G+L P+M DM G G +QMGMNMQ+ + Sbjct: 183 REKLAMGELENELITVEVEEQQPSMFDMLQGSGMEQMGMNMQDALSGLMPKKRKKRKLTV 242 Query: 242 REARKVLIQDEAAKLIDMDDVIQESVARAEQSGIIFIDEIDKVASQGKG-SGPDVSREGV 300 REARKVL +EA KLIDMD+V QE+V RAEQSGIIFIDEIDK+AS+ G S DVSREGV Sbjct: 243 REARKVLSNEEAQKLIDMDEVTQEAVFRAEQSGIIFIDEIDKIASKSSGGSNADVSREGV 302 Query: 301 QRDILPIVEGSTVMTKYGPVKTDYVLFMAAGAFHVAKPSDLIPELQGRFPIRVELSSLTL 360 QRDILPIVEGSTV+TKYG VKTD+VLFMAAGAFH+AKPSDLIPELQGRFPIRVEL+ LT+ Sbjct: 303 QRDILPIVEGSTVVTKYGSVKTDHVLFMAAGAFHIAKPSDLIPELQGRFPIRVELTKLTV 362 Query: 361 EDFVSILTEPENALTKQYVNLLQTENIEVQFQKEAIYEIARIAASVNQNMENIGARRLHT 420 EDF IL EP+NALTKQY LL+TE I+++F +AI IA +A VNQN +NIGARRLHT Sbjct: 363 EDFYKILVEPDNALTKQYTALLETEGIQIEFSDDAIRRIAEVAYEVNQNTDNIGARRLHT 422 Query: 421 ILEKLLEDLSFEAPELTLETMVITPEYVREKLAGIAQDRDLSQYIL 466 ILEKLLEDLSFEAPE+++ + ITP+YV EKL I++++DLSQ+IL Sbjct: 423 ILEKLLEDLSFEAPEISMGKVTITPQYVEEKLGAISRNKDLSQFIL 468 >Q9KA27.1 HSLU_BACHD ATP-dependent hsl protease ATP-binding subunit hslU Length = 464 Score = 574 bits (1479), Expect = e-162, Method: Compositional matrix adjust. Identities = 288/461 (62%), Positives = 346/461 (75%), Gaps = 1/461 (0%) Query: 6 LTPRQIVAELDKYIVGQKQAKKSVAVAXXXXXXXXXXXXXXXDEVVPKNILMIGPTGVGK 65 LTPRQIV LD+YIVGQ+ AK+SVA+A DE+ PKNILMIGPTGVGK Sbjct: 5 LTPRQIVERLDQYIVGQEGAKRSVAIALRNRYRRFQLEDKLRDEITPKNILMIGPTGVGK 64 Query: 66 TEIARRLAKLVNAPFIKVEATKFTEVGYVGRDVESMVRDLVETSIRMVKLERTEKVKDRA 125 TEIARRLAKLV APF+KVEATKFTEVGYVGRDVESM+RDLVETS+R+VK E+ + VK +A Sbjct: 65 TEIARRLAKLVGAPFVKVEATKFTEVGYVGRDVESMIRDLVETSVRLVKEEKMKGVKKQA 124 Query: 126 EELANERIVSILVPGGSKNKSQRNPFEMIFXXXXXXXXXXXXXXXXXSLSERRRGVKFKL 185 EE AN+RIV +LVP K ++ +NP EM+F +L+ERRR + +L Sbjct: 125 EEQANQRIVELLVPAKKKTQAYKNPLEMLFGQQQENTDHQETSAEQDTLTERRRRMSHQL 184 Query: 186 LAGQLXXXXXXXXXXXTAPTMLDMFAGQGNDQMGMNMQEMFGSXXXXXXXXXXXXIREAR 245 G+L P DM G G +QMGMNMQEM G+ +REAR Sbjct: 185 ALGELEDHYVTVEVEEQTPQFFDMLQGSGMEQMGMNMQEMLGNMMPKKRKKRKLTVREAR 244 Query: 246 KVLIQDEAAKLIDMDDVIQESVARAEQSGIIFIDEIDKVASQGKGSGPDVSREGVQRDIL 305 +VL ++EA KLIDMD+V QE+V++AEQ GI+FIDEIDK+A +G+ S DVSREGVQRDIL Sbjct: 245 RVLTEEEAQKLIDMDEVTQEAVSKAEQLGIVFIDEIDKIAGKGQQSA-DVSREGVQRDIL 303 Query: 306 PIVEGSTVMTKYGPVKTDYVLFMAAGAFHVAKPSDLIPELQGRFPIRVELSSLTLEDFVS 365 PIVEGSTV+TKYGPV TD++LF+ AGAFHVAKPSDLIPELQGRFPIRVELS+LT++DFV Sbjct: 304 PIVEGSTVVTKYGPVSTDHMLFIGAGAFHVAKPSDLIPELQGRFPIRVELSNLTVDDFVR 363 Query: 366 ILTEPENALTKQYVNLLQTENIEVQFQKEAIYEIARIAASVNQNMENIGARRLHTILEKL 425 IL EP+NAL KQY LL+TE I++ F EA+++IA IA VNQ ENIGARRLHT+LE+L Sbjct: 364 ILVEPDNALLKQYAALLETEGIKIVFTDEAVHKIATIATEVNQETENIGARRLHTLLERL 423 Query: 426 LEDLSFEAPELTLETMVITPEYVREKLAGIAQDRDLSQYIL 466 LEDLSFEAP++ LE +VITPEYV EKLA IA++RDLSQYIL Sbjct: 424 LEDLSFEAPDVNLEEIVITPEYVTEKLAAIAKNRDLSQYIL 464 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,122,683,307 Number of extensions: 76031724 Number of successful extensions: 368389 Number of sequences better than 1.0e-05: 2832 Number of HSP's gapped: 364487 Number of HSP's successfully gapped: 5004 Length of query: 466 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 327 Effective length of database: 1,751,175,118 Effective search space: 572634263586 Effective search space used: 572634263586 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)