BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0066.p (141 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIB8.1 B3KIB8_9BACI Flagellar basal-body rod protein FlgB 121 2e-26 B1DLW4.1 B1DLW4_9BACL Flagellar basal-body rod protein FlgB 119 1e-25 B7GGC3.1 B7GGC3_ANOFW Flagellar basal body rod protein FlgB 80 8e-14 B0TH99.1 B0TH99_HELMI Flagellar basal-body rod protein flgb, put... 79 1e-13 C3J3Z5.1 C3J3Z5_9BACI Flagellar basal-body rod protein FlgB 78 2e-13 >B3KIB8.1 B3KIB8_9BACI Flagellar basal-body rod protein FlgB Length = 135 Score = 121 bits (303), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 69/135 (51%), Positives = 92/135 (68%) Query: 7 LGLLNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTL 66 + LLN+ SFQRLQ GLDAA R +V+ANN+AN DTP+FKRS+V+FES L+++ +G + +L Sbjct: 1 MNLLNNSSFQRLQSGLDAANLRNNVIANNIANVDTPHFKRSEVSFESLLKKEMDGAQLSL 60 Query: 67 NAKVTDSRHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQ 126 + TD RHF G +P A+V D ++S RE +L AENQLRYNSY+ Sbjct: 61 QGRRTDPRHFVIGPTGSIPDASVIKDGSSSMNNNQNNVDADREMSLLAENQLRYNSYIQA 120 Query: 127 LNSQITMMRTVVEGR 141 +N QI MMRT +EGR Sbjct: 121 VNEQIRMMRTAIEGR 135 >B1DLW4.1 B1DLW4_9BACL Flagellar basal-body rod protein FlgB Length = 132 Score = 119 bits (297), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 63/133 (47%), Positives = 81/133 (60%), Gaps = 2/133 (1%) Query: 10 LNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAK 69 +N SF RL+G L A+ RQ V+ANN+AN DTPNFKRS+V FE L QE G ++ L K Sbjct: 1 MNGTSFGRLEGALKASELRQGVVANNIANVDTPNFKRSEVLFEELL-SQEMGGQTALQGK 59 Query: 70 VTDSRHFQFG-TGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLN 128 T+ RH G T IVPTA + TD + RE +L A+NQL YN Y+ Q+N Sbjct: 60 RTNERHIPIGRTSGIVPTAQIQTDNLSEMNNNENNVDIDREMSLLAKNQLSYNFYIQQIN 119 Query: 129 SQITMMRTVVEGR 141 + MM+T +EGR Sbjct: 120 HDVKMMKTAIEGR 132 >B7GGC3.1 B7GGC3_ANOFW Flagellar basal body rod protein FlgB Length = 126 Score = 79.7 bits (195), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 48/131 (36%), Positives = 70/131 (53%), Gaps = 9/131 (6%) Query: 10 LNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAK 69 L S +FQ L+ GL+ ++ +Q V+ANN+AN DTPNFK DV F + Q L A Sbjct: 3 LFSNTFQILEQGLNYSSLKQKVIANNIANVDTPNFKAKDVQFRTEFHQ-------ALQAY 55 Query: 70 VTDSRHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQLNS 129 TD RHF+F GT V+T +E + A+NQ+ YN+ V +LN Sbjct: 56 RTDPRHFEFKGGT--SKFFVTTKNDLVYNHNNNNVDLDKEMSDLAQNQIYYNALVERLNG 113 Query: 130 QITMMRTVVEG 140 + ++TV++G Sbjct: 114 KFNSLKTVIKG 124 >B0TH99.1 B0TH99_HELMI Flagellar basal-body rod protein flgb, putative Length = 137 Score = 79.3 bits (194), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 44/134 (32%), Positives = 70/134 (52%) Query: 7 LGLLNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTL 66 +G L + + L+ GLDAA+ R V+A+N+AN DTP +KR +V FE L + S + Sbjct: 2 IGFLETPILRSLEKGLDAASTRHQVIADNLANVDTPGYKRKEVQFEDELAAVIDREASGM 61 Query: 67 NAKVTDSRHFQFGTGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQ 126 K +D RH G + V T E+ S RE AL A+N L YN + Sbjct: 62 TGKRSDPRHIPLNGGIGDLSVRVVTQESESFRNDGNNVDVDREMALLAKNSLWYNGMINT 121 Query: 127 LNSQITMMRTVVEG 140 ++ +++ ++ V++G Sbjct: 122 ISHKLSGIKNVLQG 135 >C3J3Z5.1 C3J3Z5_9BACI Flagellar basal-body rod protein FlgB Length = 129 Score = 78.2 bits (191), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 49/134 (36%), Positives = 73/134 (54%), Gaps = 12/134 (8%) Query: 10 LNSVSFQRLQGGLDAATKRQSVLANNVANADTPNFKRSDVNFESYLQQQENGLKSTLNAK 69 L S + L+ GL A RQ +A+N+ANADTP +K DV F+S L++ L AK Sbjct: 3 LFSATIGWLEQGLRYAALRQQAIADNIANADTPGYKAKDVRFQSELER-----AFRLEAK 57 Query: 70 VTDSRHFQFG---TGTIVPTATVSTDETTSXXXXXXXXXXXREQALSAENQLRYNSYVAQ 126 T+ +HF F G I+ V+TD T S +E + AENQ+ YN+ + + Sbjct: 58 RTNPKHFSFPNEQNGKII----VTTDTTVSYNHNGNSVDIDQEMSKLAENQIYYNALIER 113 Query: 127 LNSQITMMRTVVEG 140 LN + T ++TV++G Sbjct: 114 LNGKFTTLKTVIKG 127 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.311 0.125 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 658,387,683 Number of extensions: 20840956 Number of successful extensions: 40248 Number of sequences better than 1.0e-05: 132 Number of HSP's gapped: 40278 Number of HSP's successfully gapped: 132 Length of query: 141 Length of database: 3,060,696,203 Length adjustment: 104 Effective length of query: 37 Effective length of database: 2,080,910,643 Effective search space: 76993693791 Effective search space used: 76993693791 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 126 (53.1 bits)