BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0061.p (290 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIC3.1 B3KIC3_9BACI Flagellar assembly protein FliH 309 2e-82 B1DLW9.1 B1DLW9_9BACL Flagellar assembly protein FliH 219 2e-55 C0ZF98.1 C0ZF98_BREBN Probable flagellar assembly protein FliH 135 5e-30 Q5WFQ8.1 Q5WFQ8_BACSK Flagellar assembly protein FliH 85 8e-15 A1HN40.1 A1HN40_9FIRM Flagellar assembly protein FliH 84 1e-14 >B3KIC3.1 B3KIC3_9BACI Flagellar assembly protein FliH Length = 293 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 162/289 (56%), Positives = 205/289 (70%) Query: 2 AEEMTSLSRLIKHSQYVPVDVLKRLEQARQYAGLTEESIASDEAAGEVHIQDSAREEAEQ 61 AEEMTSLS LIK S Y P+DVLK L+ AR+YA TEE++ EAE+ Sbjct: 4 AEEMTSLSNLIKSSHYKPMDVLKELDLARRYASPTEETVEEPAPPTAEPAAVQTLAEAER 63 Query: 62 ARKQMLKDAQEFAEGQVRDAAQEAENIVESXXXXXXXXXXXXXXXDEHLVEAVKSXXXXX 121 + +ML+DAQEFAE Q+R+A++EA+ ++ DE L E +K Sbjct: 64 LKHEMLEDAQEFAERQIREASEEAQRLLTEAQEQIEAWWTERRLQDEDLSETLKMEGFNQ 123 Query: 122 XXXXXXXXXXLEMSRKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDK 181 LEM M + +A ++LQ A++AKE +IQEAEPFLVELSCSIAEK++D+ Sbjct: 124 GYAEGSAKAELEMKELMEQATVQASELLQLAHQAKEDLIQEAEPFLVELSCSIAEKVLDR 183 Query: 182 QLTVEPQFAMDLIRKNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPD 241 QLT+EP+F ++LIRKNLARKRE+G+ISLCV+P QF FV AAREEL +++DSQAELQILPD Sbjct: 184 QLTIEPEFTLELIRKNLARKREKGIISLCVAPDQFEFVYAAREELGLSIDSQAELQILPD 243 Query: 242 STVKDHGCVIRSSFGSIDARIDTQLAEIKKELIRIALDTDENRNGEDDA 290 STVKD GCVIRSSFGS+DARIDTQLAEIKKEL+RIALD +E RN +++A Sbjct: 244 STVKDRGCVIRSSFGSVDARIDTQLAEIKKELVRIALDVEERRNQDEEA 292 >B1DLW9.1 B1DLW9_9BACL Flagellar assembly protein FliH Length = 278 Score = 219 bits (559), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 126/286 (44%), Positives = 179/286 (62%), Gaps = 14/286 (4%) Query: 8 LSRLIKHSQYVPVDVLKRLEQARQYAG----LTEESIASDEAAGEVHIQDSAREEAEQAR 63 +S LIK S + +D LK+L ++A +TEE I E AG EE R Sbjct: 1 MSNLIKSSSVIALDQLKQLYWLNKHAAEQVAVTEEII---EEAG-------PDEETISIR 50 Query: 64 KQMLKDAQEFAEGQVRDAAQEAENIVESXXXXXXXXXXXXXXXDEHLVEAVKSXXXXXXX 123 Q++ DAQ FAE ++R+A +++E ++ DE EA + Sbjct: 51 DQIISDAQNFAEERIREAGEQSEQLLAQAEADIDAWWLEKRLEDERHSEASRQAGFEQGY 110 Query: 124 XXXXXXXXLEMSRKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQL 183 + ++ +EEA VL+ AY +E IIQEAEPFLVELS SI+EK++ +QL Sbjct: 111 AEGQAHAEQALQQEWESRLEEANAVLKSAYEMREQIIQEAEPFLVELSVSISEKVIGRQL 170 Query: 184 TVEPQFAMDLIRKNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDST 243 + P+ A++LIRK L+R+REQG+I+LCV+P Q +FV AAREEL + +DSQAELQI+PD++ Sbjct: 171 SEAPEMALELIRKALSRRREQGVIALCVAPGQLAFVQAAREELNLVIDSQAELQIIPDAS 230 Query: 244 VKDHGCVIRSSFGSIDARIDTQLAEIKKELIRIALDTDENRNGEDD 289 VKD GCVIRS++GSIDARIDTQL+EIK+EL+++A + E R+ D+ Sbjct: 231 VKDFGCVIRSAYGSIDARIDTQLSEIKRELVQLANQSHEERSMPDE 276 >C0ZF98.1 C0ZF98_BREBN Probable flagellar assembly protein FliH Length = 272 Score = 135 bits (340), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 89/273 (32%), Positives = 142/273 (52%), Gaps = 7/273 (2%) Query: 5 MTSLSRLIKHSQYVPVDVLKRLEQARQYAGLTEESIASDEAAGEVHIQDSAREEAEQARK 64 M SLSR+IK + Y P + L +T + ++E A + +AE K Sbjct: 1 MISLSRIIKSANYRPSEESFLL-------SVTPVPLKTEEVAERLQENQEIFNQAEIEAK 53 Query: 65 QMLKDAQEFAEGQVRDAAQEAENIVESXXXXXXXXXXXXXXXDEHLVEAVKSXXXXXXXX 124 ++ DA+E A+ ++ AA++AE + + L + V+ Sbjct: 54 AIISDAEETAQNILQQAAEQAEQLRQETLAQMEEWWEQKRQEAAQLFQQVQEQAMTEGQE 113 Query: 125 XXXXXXXLEMSRKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLT 184 E + + +A+ +L++A+ K+ II EAEPF+VELS IA+KI+ +++ Sbjct: 114 AGFETGKREAYEEQTASLVQAKTILEQAFAEKKQIIAEAEPFVVELSIEIAKKIIGQEVQ 173 Query: 185 VEPQFAMDLIRKNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTV 244 P+ +++ +K L R R G I++CV+ F +V R +D QAEL I PD TV Sbjct: 174 QHPEQVLEMTKKALRRSRVHGEITVCVNHKYFDYVLEHRAGFLELLDGQAELSIYPDYTV 233 Query: 245 KDHGCVIRSSFGSIDARIDTQLAEIKKELIRIA 277 +D GCVIR++ GS+DARIDTQ+ EIK+ L+ IA Sbjct: 234 QDGGCVIRTALGSVDARIDTQMEEIKQALLTIA 266 >Q5WFQ8.1 Q5WFQ8_BACSK Flagellar assembly protein FliH Length = 256 Score = 84.7 bits (208), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 43/130 (33%), Positives = 80/130 (61%), Gaps = 1/130 (0%) Query: 144 EARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMDLIRKNLARKRE 203 EA+QVL+EA A + + + +P L+EL+C+IAEK++ + +P + +++R LA R+ Sbjct: 115 EAKQVLEEAQAAAALKVDKEQPLLIELACAIAEKVIGVSIAEDP-YLQNIVRAALAEVRD 173 Query: 204 QGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIRSSFGSIDARID 263 + L V P + + A +EL A+ + + Q++PD ++D C I ++ G +DA + Sbjct: 174 HAFVKLYVPPHWYKRLAACVDELQGAIPACEDFQLIPDGQLQDDHCFIVTNAGRMDASLP 233 Query: 264 TQLAEIKKEL 273 TQ+ ++KK+L Sbjct: 234 TQIEQLKKQL 243 >A1HN40.1 A1HN40_9FIRM Flagellar assembly protein FliH Length = 258 Score = 84.3 bits (207), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 45/141 (31%), Positives = 82/141 (58%) Query: 133 EMSRKMAEMMEEARQVLQEAYRAKEVIIQEAEPFLVELSCSIAEKIVDKQLTVEPQFAMD 192 EM + + ++A Q+L A R + +AE +++L+ ++A KI+ +++ P + Sbjct: 113 EMQGALRQAADQAGQLLAAAEREYRQTLLDAERHIIDLALAVARKILAREIEENPMVVLP 172 Query: 193 LIRKNLARKREQGLISLCVSPAQFSFVNAAREELAMAVDSQAELQILPDSTVKDHGCVIR 252 ++R L + R+Q I++ V+P F V A+ +L + + + LQI+ D+TV GC++ Sbjct: 173 IVRAALEKVRDQEQITILVNPEDFGVVLEAKRDLELVIGRERALQIVADNTVGRGGCLLD 232 Query: 253 SSFGSIDARIDTQLAEIKKEL 273 + FG +DARIDTQ IK+ L Sbjct: 233 TPFGMVDARIDTQFEMIKRVL 253 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.127 0.326 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,224,440,480 Number of extensions: 40564653 Number of successful extensions: 139719 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 139747 Number of HSP's successfully gapped: 99 Length of query: 290 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 156 Effective length of database: 1,798,280,193 Effective search space: 280531710108 Effective search space used: 280531710108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)