BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0053.p (261 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KID0.1 B3KID0_9BACI Putative uncharacterized protein 399 e-109 B1DLL7.1 B1DLL7_9BACL Putative uncharacterized protein 355 2e-96 C0ZF92.1 C0ZF92_BREBN Flagellar hook protein FlgE 288 2e-76 C5D8X2.1 C5D8X2_9BACI Putative uncharacterized protein 253 1e-65 Q5L0L8.1 Q5L0L8_GEOKA Flagellar hook protein 251 4e-65 >B3KID0.1 B3KID0_9BACI Putative uncharacterized protein Length = 274 Score = 399 bits (1025), Expect = e-109, Method: Compositional matrix adjust. Identities = 197/263 (74%), Positives = 227/263 (86%), Gaps = 2/263 (0%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 MRGFQTKLDVIGNNIANVNT+GFK+GRVMFKDI+SQT+SGVT D GGVNAKQIGLG Sbjct: 12 MRGFQTKLDVIGNNIANVNTVGFKAGRVMFKDILSQTMSGVTPGTDDPSGGVNAKQIGLG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 VSIGSIDT+H GSAM TNNP DLRIDGDGFF+VKLS++Q PFLTRAGDFH+D ARNL+ Sbjct: 72 VSIGSIDTLHTPGSAMMTNNPRDLRIDGDGFFMVKLSEDQEVPFLTRAGDFHIDVARNLV 131 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 TSDG+ VL+SAG+PIQL+E T+F+I+ +G+++Q + DGT + IGVAK+ NP+GLEK Sbjct: 132 TSDGMLVLDSAGDPIQLDEEVTSFTIAQNGAIIQKMSDGTTEEGAIIGVAKIPNPEGLEK 191 Query: 181 IGGNLYRMTLNANADGALNPTT--ANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRG 238 IGGN+YR+TLNA DG L NN EEGTGAI+AGQLEMSNVDLTGEF+EMIV+QRG Sbjct: 192 IGGNMYRVTLNAVPDGDLAAVMFEGNNAEEGTGAIIAGQLEMSNVDLTGEFSEMIVAQRG 251 Query: 239 FQANSRIITTSDEVLQEVVNLKR 261 FQANSRIITTSDEVLQEVVNLKR Sbjct: 252 FQANSRIITTSDEVLQEVVNLKR 274 >B1DLL7.1 B1DLL7_9BACL Putative uncharacterized protein Length = 270 Score = 355 bits (912), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 183/261 (70%), Positives = 211/261 (80%), Gaps = 2/261 (0%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 MRGFQTKLDVIGNNIANVNT+GFK GRVMF DI+SQT SGVTA ++G QGGVNAKQIGLG Sbjct: 12 MRGFQTKLDVIGNNIANVNTVGFKGGRVMFSDILSQTTSGVTASLEGEQGGVNAKQIGLG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 V+I +IDT+H GSAMTTN PTDLRIDGDGFF V + T +LTRAG+F +D R L+ Sbjct: 72 VTISAIDTIHTPGSAMTTNVPTDLRIDGDGFFAVSAGGDSPT-YLTRAGNFTLDGNRQLV 130 Query: 121 TSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLEK 180 +DG+ VL+S GEPI L E T+FSI DGS+L DGT ++ ++GV KV NP GLEK Sbjct: 131 NADGMFVLSSDGEPIVLGEDVTSFSIGQDGSILAVTADGT-EAVAQLGVVKVVNPSGLEK 189 Query: 181 IGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGFQ 240 +GGNLYR+T NAN DG TA++ E GTGAI+AGQLEMSNVDLT EFTEMIV+QRGFQ Sbjct: 190 VGGNLYRVTANANPDGEFEIVTADDPELGTGAIIAGQLEMSNVDLTNEFTEMIVAQRGFQ 249 Query: 241 ANSRIITTSDEVLQEVVNLKR 261 ANSRIITTSDE+LQEVVNLKR Sbjct: 250 ANSRIITTSDEILQEVVNLKR 270 >C0ZF92.1 C0ZF92_BREBN Flagellar hook protein FlgE Length = 272 Score = 288 bits (738), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 155/263 (58%), Positives = 190/263 (72%), Gaps = 4/263 (1%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 +RGFQTKLDVIGNNIANVNT+GFK GRVMF+DI+SQ V G AP GG+GG N QIGLG Sbjct: 12 LRGFQTKLDVIGNNIANVNTVGFKKGRVMFQDILSQNVQGAGAPTAGGKGGTNPTQIGLG 71 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNLL 120 SI SIDT+ AGS MTTN PTDL I+GDGFF+V DE AT + TRAG+F D+ L+ Sbjct: 72 ASIASIDTVFSAGSPMTTNLPTDLSIEGDGFFMVS-PDEGATIYYTRAGNFTRDAEGYLV 130 Query: 121 TSDGLHVLNSAGEPIQL--NEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGL 178 S G+ ++N+ IQ+ N+G ++SI DG + + G +DS IGV +NP GL Sbjct: 131 NSQGMKLMNTDPAAIQISQNDGVVSYSIGKDGVITTVDETGALDSTNTIGVMTFNNPGGL 190 Query: 179 EKIGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRG 238 +KIG NLY T+NA A+ +P T + +I+AGQLEMSNVDL+ EFTEMIV+QRG Sbjct: 191 KKIGNNLYENTVNAGANDG-DPMTPVTPADAKVSIIAGQLEMSNVDLSEEFTEMIVAQRG 249 Query: 239 FQANSRIITTSDEVLQEVVNLKR 261 FQANSRIITTSD VL+E+VNLKR Sbjct: 250 FQANSRIITTSDTVLEELVNLKR 272 >C5D8X2.1 C5D8X2_9BACI Putative uncharacterized protein Length = 264 Score = 253 bits (646), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 139/262 (53%), Positives = 180/262 (68%), Gaps = 10/262 (3%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDGGQGGVNAKQIGLG 60 MR FQTKLDVIGNNIANVNT GFK GR +FKD++SQ ++G + P +GGVN KQ+GLG Sbjct: 12 MRNFQTKLDVIGNNIANVNTYGFKKGRTIFKDLVSQQIAGASGPT-ANRGGVNPKQVGLG 70 Query: 61 VSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQA-TPFLTRAGDFHVDSARNL 119 + +IDT+H GS TT P DL I GDGFF+ L D+ TRAG+F++D N+ Sbjct: 71 SQLAAIDTVHTQGSLQTTGRPLDLAISGDGFFV--LGDKNGGNHVYTRAGNFYLDKDGNI 128 Query: 120 LTSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLE 179 + +DGL++L + I + +FSI +DG+V +++G++ S +I +AK +N GLE Sbjct: 129 VNADGLYLLGIGNKLINIPSSAESFSIGSDGTV-NYIENGSLKSAGQIQLAKFNNNDGLE 187 Query: 180 KIGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGF 239 K G NL+R T N+ TT GTG IVAG LEMSNVDL EFTEMIV+QRGF Sbjct: 188 KAGDNLFRQTANSGT-----ATTGAPGANGTGTIVAGTLEMSNVDLAEEFTEMIVAQRGF 242 Query: 240 QANSRIITTSDEVLQEVVNLKR 261 QAN+RIITTSDE+LQE+VNLK+ Sbjct: 243 QANTRIITTSDEILQELVNLKK 264 >Q5L0L8.1 Q5L0L8_GEOKA Flagellar hook protein Length = 263 Score = 251 bits (641), Expect = 4e-65, Method: Compositional matrix adjust. Identities = 140/262 (53%), Positives = 175/262 (66%), Gaps = 7/262 (2%) Query: 1 MRGFQTKLDVIGNNIANVNTIGFKSGRVMFKDIMSQTVSGVTAPVDG-GQGGVNAKQIGL 59 MR FQTKLDVIGNNIANVNT GFK GR +FKD+MSQT+SG + P G G+GG N KQ+GL Sbjct: 8 MRNFQTKLDVIGNNIANVNTYGFKKGRTIFKDLMSQTISGASGPNAGAGRGGTNPKQVGL 67 Query: 60 GVSIGSIDTMHLAGSAMTTNNPTDLRIDGDGFFLVKLSDEQATPFLTRAGDFHVDSARNL 119 G + +IDT+H GS TT DL I GDGFF+V + + TRAG+F++DS + Sbjct: 68 GSQLAAIDTVHTQGSLQTTGRVLDLAISGDGFFVVGDASGNNRMY-TRAGNFYLDSQGYI 126 Query: 120 LTSDGLHVLNSAGEPIQLNEGTTAFSISADGSVLQTLQDGTIDSDTKIGVAKVSNPQGLE 179 + +DG ++L +Q+ + SI ADG V GT+ + I +AK +N GLE Sbjct: 127 VNADGQYLLGVGNSRLQIPTDAKSLSIGADGKVTIVDASGTLSTIGTIQLAKFANNDGLE 186 Query: 180 KIGGNLYRMTLNANADGALNPTTANNTEEGTGAIVAGQLEMSNVDLTGEFTEMIVSQRGF 239 K G NL+R T N+ PTT GTG IV+G LEMSNVDL EFTEMIV+QRGF Sbjct: 187 KAGNNLFRETTNSGT-----PTTGAPGANGTGTIVSGALEMSNVDLAEEFTEMIVAQRGF 241 Query: 240 QANSRIITTSDEVLQEVVNLKR 261 QAN+RIITTSDE+LQE+VNLK+ Sbjct: 242 QANTRIITTSDEILQELVNLKK 263 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,511,987,083 Number of extensions: 64802248 Number of successful extensions: 150801 Number of sequences better than 1.0e-05: 2494 Number of HSP's gapped: 145371 Number of HSP's successfully gapped: 3267 Length of query: 261 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 128 Effective length of database: 1,807,701,208 Effective search space: 231385754624 Effective search space used: 231385754624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)