BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0049.p (422 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KID4.1 B3KID4_9BACI Flagellar motor switch protein FliN 379 e-103 B1DLM2.1 B1DLM2_9BACL CheC, inhibitor of MCP methylation / FliN ... 345 7e-93 C0ZF88.1 C0ZF88_BREBN Flagellar motor switch protein FliY 290 2e-76 B7GGA4.1 B7GGA4_ANOFW Flagellar motor switch protein FliY 273 2e-71 Q9KA45.1 Q9KA45_BACHD Flagellar motor switch protein 271 7e-71 >B3KID4.1 B3KID4_9BACI Flagellar motor switch protein FliN Length = 420 Score = 379 bits (974), Expect = e-103, Method: Compositional matrix adjust. Identities = 191/233 (81%), Positives = 205/233 (87%), Gaps = 2/233 (0%) Query: 1 LTSKDYLSQEEIDALLRQSAEGPVA--PSTKSVDDFLTPFEQDALGEIGNITFGSAATAL 58 +TSKDYLSQEEIDALL+QS A PS +VDD LTP EQDALGEIGNITFGSAATAL Sbjct: 1 MTSKDYLSQEEIDALLKQSEMASPASIPSAATVDDVLTPLEQDALGEIGNITFGSAATAL 60 Query: 59 STLLGKKVDITTPEVSIITRDEFEEAFPKPHVAVHVQYVDGFQGINSLVIKIRDAQVIAD 118 STLLGKKVDITTP VSII R EFEE FPKPHVAVHV+YVDGF+GINSLVIK RDAQVIAD Sbjct: 61 STLLGKKVDITTPRVSIIKRSEFEEEFPKPHVAVHVKYVDGFEGINSLVIKTRDAQVIAD 120 Query: 119 LMLGGEGEPKDEDLNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNMSSG 178 LMLGGEG P +E+LNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNM++G Sbjct: 121 LMLGGEGNPMEEELNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNMANG 180 Query: 179 EGVGSLPGDETLIQISFRLRIGELIDSTIMQLLPVQFAKDMVSMLLGDVQSEQ 231 EGV +LP DETLI+ISFRL IGELIDSTIMQLLPV F+KDMV ML+ + Q Sbjct: 181 EGVNNLPPDETLIKISFRLIIGELIDSTIMQLLPVHFSKDMVHMLINGTEEPQ 233 Score = 184 bits (468), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 90/99 (90%), Positives = 94/99 (94%) Query: 324 FANLSSGAFGNIDENNLNLLMDIPLRVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 383 FANL S + +DENNLNLLMDIPL+VTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV Sbjct: 322 FANLQSAPYTQVDENNLNLLMDIPLKVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 381 Query: 384 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 422 DILVN+KLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ Sbjct: 382 DILVNHKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 420 >B1DLM2.1 B1DLM2_9BACL CheC, inhibitor of MCP methylation / FliN fusion protein Length = 421 Score = 345 bits (884), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 173/234 (73%), Positives = 200/234 (85%), Gaps = 4/234 (1%) Query: 1 LTSKDYLSQEEIDALLRQSAEGPVAPSTKS----VDDFLTPFEQDALGEIGNITFGSAAT 56 +TSKDYLSQEEIDALL Q+A + +++ + DFL+P EQDALGEIGNITFGSAAT Sbjct: 1 MTSKDYLSQEEIDALLNQTASELASSQSQASEYQLSDFLSPIEQDALGEIGNITFGSAAT 60 Query: 57 ALSTLLGKKVDITTPEVSIITRDEFEEAFPKPHVAVHVQYVDGFQGINSLVIKIRDAQVI 116 ALSTLLGKKVDITTP+V+ I R++ FPKPHVAV VQYVDGFQGINSLVIK +DAQVI Sbjct: 61 ALSTLLGKKVDITTPQVTFIKREDLANEFPKPHVAVSVQYVDGFQGINSLVIKTKDAQVI 120 Query: 117 ADLMLGGEGEPKDEDLNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNMS 176 ADLMLGGEG+ ++LNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDIL++ Sbjct: 121 ADLMLGGEGDVTLQELNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILDVD 180 Query: 177 SGEGVGSLPGDETLIQISFRLRIGELIDSTIMQLLPVQFAKDMVSMLLGDVQSE 230 SG G+G LP ++ I+ISFRL IG+LIDSTIMQLLPV FAK MV++L+GDV+ E Sbjct: 181 SGGGMGQLPTEDVFIKISFRLTIGDLIDSTIMQLLPVPFAKQMVAILMGDVEEE 234 Score = 176 bits (446), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 86/99 (86%), Positives = 91/99 (91%) Query: 324 FANLSSGAFGNIDENNLNLLMDIPLRVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 383 FAN + + DE NLNLL+DIPL+VTVELGRTQKQIKDILE+SQGSIIELDKLAGEPV Sbjct: 323 FANFNQAPYVQTDETNLNLLLDIPLKVTVELGRTQKQIKDILELSQGSIIELDKLAGEPV 382 Query: 384 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 422 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ Sbjct: 383 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 421 >C0ZF88.1 C0ZF88_BREBN Flagellar motor switch protein FliY Length = 455 Score = 290 bits (741), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 145/231 (62%), Positives = 186/231 (80%), Gaps = 9/231 (3%) Query: 3 SKDYLSQEEIDALLR-----QSAEGPVAPSTKSVDDFLTPFEQDALGEIGNITFGSAATA 57 S+D LSQ+EIDALLR + E V+ T+ V +FL+PFEQDALGEIGNI+FGSAATA Sbjct: 2 SRDMLSQDEIDALLRANNLVEDEEEAVSAVTQDVAEFLSPFEQDALGEIGNISFGSAATA 61 Query: 58 LSTLLGKKVDITTPEVSIITRDEFEEAFPKPHVAVHVQYVDGFQGINSLVIKIRDAQVIA 117 LSTLL +KVDITTP VS++ DE E FP PHVAVHV+Y DGF+GIN LVI++ DA VIA Sbjct: 62 LSTLLSQKVDITTPTVSVVNVDELESEFPIPHVAVHVEYTDGFKGINLLVIRMEDAAVIA 121 Query: 118 DLMLGGEGEPKDEDLNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNMSS 177 DLM+GG+G P +++E+HISAVQEAMNQMMGS+ATSMSTIFN+F+NI+PPGID+++++ Sbjct: 122 DLMMGGDGRPASTEMSELHISAVQEAMNQMMGSAATSMSTIFNQFINITPPGIDVMDLAQ 181 Query: 178 GEGVGSLPGDET-LIQISFRLRI---GELIDSTIMQLLPVQFAKDMVSMLL 224 G+G S D++ L+++SFRL++ GELIDS IMQLLP+ FAK M+ L+ Sbjct: 182 GKGGDSFSDDDSPLVKVSFRLKVGEHGELIDSNIMQLLPLSFAKKMIDRLV 232 Score = 144 bits (363), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 74/99 (74%), Positives = 83/99 (83%) Query: 324 FANLSSGAFGNIDENNLNLLMDIPLRVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 383 FA L D NLNLL DIPL+VTVELGRT+K I++IL++S GSII+LDKLAGE V Sbjct: 357 FAPLGGAPMYTGDIQNLNLLHDIPLQVTVELGRTKKLIREILDLSAGSIIQLDKLAGEHV 416 Query: 384 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 422 DILVNNKLIAKGEVVVIDENFGVRVTDI+SQ DR+QKLQ Sbjct: 417 DILVNNKLIAKGEVVVIDENFGVRVTDIISQIDRVQKLQ 455 >B7GGA4.1 B7GGA4_ANOFW Flagellar motor switch protein FliY Length = 383 Score = 273 bits (698), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 136/233 (58%), Positives = 176/233 (75%) Query: 1 LTSKDYLSQEEIDALLRQSAEGPVAPSTKSVDDFLTPFEQDALGEIGNITFGSAATALST 60 + S D LSQ+EIDALLR E P+ ST S+D+ LTP EQDALGEIGNI+FGS+ATALS Sbjct: 1 MMSDDMLSQDEIDALLRGIDEDPMPSSTMSIDEVLTPIEQDALGEIGNISFGSSATALSM 60 Query: 61 LLGKKVDITTPEVSIITRDEFEEAFPKPHVAVHVQYVDGFQGINSLVIKIRDAQVIADLM 120 LL +KV+ITTP VS++ R + E FP P+VA+ V Y +GF G N LVIK DA +IADLM Sbjct: 61 LLNQKVEITTPNVSLVERAKVAEEFPHPYVAIQVSYTEGFLGTNLLVIKQSDAAIIADLM 120 Query: 121 LGGEGEPKDEDLNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDILNMSSGEG 180 +GG+G ++EI +SAVQEAMNQMMGS+ATSMSTIF++ V+ISPP + +L++ GEG Sbjct: 121 MGGDGTNPPAFMDEIQLSAVQEAMNQMMGSAATSMSTIFSKKVDISPPSLHLLDLKEGEG 180 Query: 181 VGSLPGDETLIQISFRLRIGELIDSTIMQLLPVQFAKDMVSMLLGDVQSEQET 233 LP D+ L+++SFRL++G LIDS IMQLLP+ FAK++V LL ++ ET Sbjct: 181 FEYLPPDDVLVKVSFRLKVGNLIDSNIMQLLPIHFAKELVENLLNPSSTKTET 233 Score = 142 bits (358), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 68/99 (68%), Positives = 82/99 (82%) Query: 324 FANLSSGAFGNIDENNLNLLMDIPLRVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 383 FA+ + NL++L+DIPL+VTVELGRT++ ++DIL +S GSIIELDKLAGEPV Sbjct: 285 FASFEPAPLPEAESRNLDMLLDIPLQVTVELGRTKRSVQDILSLSSGSIIELDKLAGEPV 344 Query: 384 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 422 DILVNNKLIAKGEVVVIDENFGVRVT I+SQ DR+ KL+ Sbjct: 345 DILVNNKLIAKGEVVVIDENFGVRVTSIISQSDRLNKLR 383 >Q9KA45.1 Q9KA45_BACHD Flagellar motor switch protein Length = 401 Score = 271 bits (694), Expect = 7e-71, Method: Compositional matrix adjust. Identities = 137/238 (57%), Positives = 185/238 (77%), Gaps = 12/238 (5%) Query: 5 DYLSQEEIDALLR---------QSAEGPVAPSTK---SVDDFLTPFEQDALGEIGNITFG 52 + LSQ+EI+ LL+ + +G AP + S++ FLT E+DALGEIGNI+FG Sbjct: 4 EMLSQDEINELLKGVASDGDDAEEVDGLDAPENQKGDSLEGFLTEVEKDALGEIGNISFG 63 Query: 53 SAATALSTLLGKKVDITTPEVSIITRDEFEEAFPKPHVAVHVQYVDGFQGINSLVIKIRD 112 SAATALSTLL +KVDITTP+VS+I +D+ E+ FP+PHVAVHV Y +GF+G+N LVI+ +D Sbjct: 64 SAATALSTLLNEKVDITTPQVSVIHKDQLEKEFPQPHVAVHVDYTEGFEGMNLLVIRTKD 123 Query: 113 AQVIADLMLGGEGEPKDEDLNEIHISAVQEAMNQMMGSSATSMSTIFNRFVNISPPGIDI 172 A +IADLMLGG+G +E+LN++HISAVQEAMNQMMGS++TSMSTIFN+ ++ISPPG+++ Sbjct: 124 AAIIADLMLGGDGLNPEEELNDMHISAVQEAMNQMMGSASTSMSTIFNKRIDISPPGVNM 183 Query: 173 LNMSSGEGVGSLPGDETLIQISFRLRIGELIDSTIMQLLPVQFAKDMVSMLLGDVQSE 230 L++ + +G +P + LI++SFRLRIGELIDS+IMQL+ V FAK MV L+ Q E Sbjct: 184 LDVGNDQGTDRVPEGDVLIKVSFRLRIGELIDSSIMQLVTVPFAKKMVDDLMNPAQDE 241 Score = 139 bits (350), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 68/99 (68%), Positives = 83/99 (83%) Query: 324 FANLSSGAFGNIDENNLNLLMDIPLRVTVELGRTQKQIKDILEMSQGSIIELDKLAGEPV 383 F N + + NL++L+DIPL+VTVELGRT++ IKDIL +QGSIIELDKLAGEPV Sbjct: 303 FTNFEHPELNDTEAGNLDMLLDIPLQVTVELGRTKRSIKDILSFTQGSIIELDKLAGEPV 362 Query: 384 DILVNNKLIAKGEVVVIDENFGVRVTDIVSQWDRIQKLQ 422 DILVN KLIAKGEVVVIDENFGVRVT+I+SQ +RI++L+ Sbjct: 363 DILVNQKLIAKGEVVVIDENFGVRVTEILSQRERIEQLK 401 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.135 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,812,199,708 Number of extensions: 69309897 Number of successful extensions: 226552 Number of sequences better than 1.0e-05: 1020 Number of HSP's gapped: 225751 Number of HSP's successfully gapped: 1200 Length of query: 422 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 284 Effective length of database: 1,760,596,133 Effective search space: 500009301772 Effective search space used: 500009301772 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)