BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0046.p (255 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KID7.1 B3KID7_9BACI Flagellar biosynthetic protein FliP EC=3.1... 281 3e-74 B1DLM5.1 B1DLM5_9BACL Flagellar biosynthetic protein FliP EC=3.1... 277 6e-73 Q9KA48.1 Q9KA48_BACHD Flagellar protein required for flagellar f... 244 7e-63 P35528.1 FLIP_BACSU Flagellar biosynthetic protein fliP 239 1e-61 A7Z4Q6.1 A7Z4Q6_BACA2 FliP 238 4e-61 >B3KID7.1 B3KID7_9BACI Flagellar biosynthetic protein FliP EC=3.1.1.3 Length = 250 Score = 281 bits (720), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 142/194 (73%), Positives = 158/194 (81%), Gaps = 3/194 (1%) Query: 61 APAFLVLMTSFTRIVIVLGFVRTSLGTQSMPPNQVLVGLALFLTLFIMSPTLAKVNETAL 120 APA LVLMTSFTRIVIVLGFVRTSLGTQ PPNQVL+GLALFLTLF+M+PTL+ +NE AL Sbjct: 59 APAILVLMTSFTRIVIVLGFVRTSLGTQQTPPNQVLIGLALFLTLFVMAPTLSSMNEVAL 118 Query: 121 QPYMKGTITQTEALNKAADPMKEFMFKQTSTKDLLLFMNYTGNKASVKPATYSDIPLTVM 180 QPY+KG ITQ EAL+KA DPMK+FMF T KDLLLFMNYTG + KP +Y DIPLTV+ Sbjct: 119 QPYLKGEITQAEALDKAQDPMKKFMFAHTREKDLLLFMNYTGAE---KPESYEDIPLTVL 175 Query: 181 VPAFAIGEMKKAFTMGFMIFIPFLIIDIVVASTXXXXXXXXXXXXXISLPFKIMLFVLVD 240 VPAFAI E+K F MGFMIFIPFL+IDIVVAS ISLPFKI+LFVLVD Sbjct: 176 VPAFAISELKTGFQMGFMIFIPFLVIDIVVASVLMAMGMMMLPPVMISLPFKILLFVLVD 235 Query: 241 GWYLVVKSLLLSFN 254 GWYLVVKS+L+SFN Sbjct: 236 GWYLVVKSMLMSFN 249 >B1DLM5.1 B1DLM5_9BACL Flagellar biosynthetic protein FliP EC=3.1.1.3 Length = 256 Score = 277 bits (709), Expect = 6e-73, Method: Compositional matrix adjust. Identities = 139/195 (71%), Positives = 158/195 (81%), Gaps = 3/195 (1%) Query: 61 APAFLVLMTSFTRIVIVLGFVRTSLGTQSMPPNQVLVGLALFLTLFIMSPTLAKVNETAL 120 APA LVLMTSFTRIVIVLGFVRTSLGTQ MPPNQVL+GLA+FLT FIM+PT ++VNE AL Sbjct: 65 APAILVLMTSFTRIVIVLGFVRTSLGTQQMPPNQVLIGLAMFLTFFIMAPTFSQVNEVAL 124 Query: 121 QPYMKGTITQTEALNKAADPMKEFMFKQTSTKDLLLFMNYTGNKASVKPATYSDIPLTVM 180 QPY+KG ITQT AL KAADPMK+FM T KDLLLF+NYT + KP Y DIP+TV+ Sbjct: 125 QPYLKGEITQTVALEKAADPMKKFMISHTREKDLLLFLNYTKTE---KPEKYQDIPITVL 181 Query: 181 VPAFAIGEMKKAFTMGFMIFIPFLIIDIVVASTXXXXXXXXXXXXXISLPFKIMLFVLVD 240 VPA+AI E+K AF MGFMIFIPFL+ID++VAST ISLPFK++LFVLVD Sbjct: 182 VPAYAISELKTAFQMGFMIFIPFLVIDMIVASTLMAMGMMMLPPVMISLPFKLLLFVLVD 241 Query: 241 GWYLVVKSLLLSFNT 255 GWYL+VKSLLLSFNT Sbjct: 242 GWYLIVKSLLLSFNT 256 >Q9KA48.1 Q9KA48_BACHD Flagellar protein required for flagellar formation Length = 233 Score = 244 bits (622), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 119/194 (61%), Positives = 147/194 (75%), Gaps = 3/194 (1%) Query: 61 APAFLVLMTSFTRIVIVLGFVRTSLGTQSMPPNQVLVGLALFLTLFIMSPTLAKVNETAL 120 AP+ L+LMT FTR+VIVL FVRT L TQ+MPPNQV++G+ALF+T FIM+P A+VN+ AL Sbjct: 43 APSILILMTCFTRVVIVLSFVRTGLATQTMPPNQVIIGIALFITFFIMAPVFAEVNDQAL 102 Query: 121 QPYMKGTITQTEALNKAADPMKEFMFKQTSTKDLLLFMNYTGNKASVKPATYSDIPLTVM 180 P++ G I Q EA+++AA PMKEFM K T KDL LFM Y G+ +P + DIPLT + Sbjct: 103 TPFLDGEIDQQEAMDRAAIPMKEFMAKHTREKDLALFMGYAGHD---RPESIEDIPLTAL 159 Query: 181 VPAFAIGEMKKAFTMGFMIFIPFLIIDIVVASTXXXXXXXXXXXXXISLPFKIMLFVLVD 240 VPAFAI E+K AF +GF+IFIPFL+ID++VAS ISLPFKI+LFVLVD Sbjct: 160 VPAFAISELKTAFQIGFLIFIPFLVIDMIVASVLMSMGMMMLPPIMISLPFKILLFVLVD 219 Query: 241 GWYLVVKSLLLSFN 254 GWYLVV+SLLLSFN Sbjct: 220 GWYLVVQSLLLSFN 233 >P35528.1 FLIP_BACSU Flagellar biosynthetic protein fliP Length = 221 Score = 239 bits (611), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 125/193 (64%), Positives = 141/193 (73%), Gaps = 3/193 (1%) Query: 61 APAFLVLMTSFTRIVIVLGFVRTSLGTQSMPPNQVLVGLALFLTLFIMSPTLAKVNETAL 120 AP L+LMT FTRIVIVL FVRTSL TQSMPPNQVL+GLALFLT FIM+PT +++N+ AL Sbjct: 32 APGILILMTCFTRIVIVLSFVRTSLATQSMPPNQVLIGLALFLTFFIMAPTFSEINKEAL 91 Query: 121 QPYMKGTITQTEALNKAADPMKEFMFKQTSTKDLLLFMNYTGNKASVKPATYSDIPLTVM 180 P M I+ EA KA +P+KEFM K T KDL LFMNY KP + DIPLT M Sbjct: 92 TPLMDNKISLDEAYTKAEEPIKEFMSKHTRQKDLALFMNYAKMD---KPESLKDIPLTTM 148 Query: 181 VPAFAIGEMKKAFTMGFMIFIPFLIIDIVVASTXXXXXXXXXXXXXISLPFKIMLFVLVD 240 VPAFAI E+K AF +GFMIFIPFLIID+VVAS ISLPFKI+LFVLVD Sbjct: 149 VPAFAISELKTAFQIGFMIFIPFLIIDMVVASVLMSMGMMMLPPVMISLPFKILLFVLVD 208 Query: 241 GWYLVVKSLLLSF 253 GWYL+VKSLL SF Sbjct: 209 GWYLIVKSLLQSF 221 >A7Z4Q6.1 A7Z4Q6_BACA2 FliP Length = 221 Score = 238 bits (607), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 124/193 (64%), Positives = 141/193 (73%), Gaps = 3/193 (1%) Query: 61 APAFLVLMTSFTRIVIVLGFVRTSLGTQSMPPNQVLVGLALFLTLFIMSPTLAKVNETAL 120 AP L+LMT FTRIVIVL FVRTSL TQ+MPPNQVL+GLALFLT FIM+PT +++N+ AL Sbjct: 32 APGILILMTCFTRIVIVLSFVRTSLATQNMPPNQVLIGLALFLTFFIMAPTFSEINKEAL 91 Query: 121 QPYMKGTITQTEALNKAADPMKEFMFKQTSTKDLLLFMNYTGNKASVKPATYSDIPLTVM 180 P M I+ EA KA P+KE+M K T KDL LFMNY K KP + DIPLT M Sbjct: 92 TPLMDNKISLDEAYTKAEKPIKEYMSKHTRQKDLALFMNYAKMK---KPESIQDIPLTTM 148 Query: 181 VPAFAIGEMKKAFTMGFMIFIPFLIIDIVVASTXXXXXXXXXXXXXISLPFKIMLFVLVD 240 VPA+AI E+K AF MGFMIFIPFLIID+VVAS ISLPFKI+LFVLVD Sbjct: 149 VPAYAISELKTAFQMGFMIFIPFLIIDMVVASVLMSMGMMMLPPVMISLPFKILLFVLVD 208 Query: 241 GWYLVVKSLLLSF 253 GWYL+VKSLL SF Sbjct: 209 GWYLIVKSLLDSF 221 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.138 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 977,897,803 Number of extensions: 30502251 Number of successful extensions: 101915 Number of sequences better than 1.0e-05: 1357 Number of HSP's gapped: 100350 Number of HSP's successfully gapped: 1357 Length of query: 255 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 122 Effective length of database: 1,807,701,208 Effective search space: 220539547376 Effective search space used: 220539547376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)