BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0043.p (364 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE0.1 B3KIE0_9BACI Flagellar biosynthetic protein FlhB 488 e-136 B1DLM8.1 B1DLM8_9BACL Flagellar biosynthetic protein FlhB 392 e-107 C0ZF82.1 C0ZF82_BREBN Flagellar biosynthetic protein FlhB 345 3e-93 B4ADY7.1 B4ADY7_BACPU Flagellar biosynthetic protein FlhB 340 1e-91 A8FDA3.1 A8FDA3_BACP2 Flagellar biosynthesis protein FlhB 338 4e-91 >B3KIE0.1 B3KIE0_9BACI Flagellar biosynthetic protein FlhB Length = 362 Score = 488 bits (1255), Expect = e-136, Method: Compositional matrix adjust. Identities = 233/359 (64%), Positives = 283/359 (78%) Query: 6 RYALNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKER 65 RY ++LQ F G+KTEKATPKKRQ+ RKKGQVAK ++FGG++++R Sbjct: 4 RYPVDLQLFAGEKTEKATPKKRQDTRKKGQVAKSMEVSGASVLLAAFFCLLIFGGYMRDR 63 Query: 66 VVKLYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMM 125 V+L+TD++ NR+ M VT +NV E+F YG+QIL+LLAPLFI FV+ALV N +VGFM+ Sbjct: 64 TVRLFTDIYINRLSMNVTKDNVMELFGEYGVQILLLLAPLFISIFVIALVANYLQVGFML 123 Query: 126 VTEGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAH 185 E L KFSKI+PIKGFKNIFSMRS VEFLKS+ KL IIGYLVYSTLW +E +SL+ Sbjct: 124 TGETLKMKFSKIDPIKGFKNIFSMRSLVEFLKSVIKLTIIGYLVYSTLWAAREDLSSLST 183 Query: 186 VSAEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEG 245 + + + F A +TM+LG KI L+I+AV DY+YQKY++EK+++MSKQDIKDEYKKMEG Sbjct: 184 MGPDTIFHFVADITMSLGFKIAIGLIILAVLDYMYQKYDYEKNIRMSKQDIKDEYKKMEG 243 Query: 246 DPIIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDF 305 DP+IKGKIRE EVPNADVIITNPTHFAVA+KYDGS MEAPQIIAKGQDF Sbjct: 244 DPLIKGKIRERQRRMALQRMMQEVPNADVIITNPTHFAVAIKYDGSTMEAPQIIAKGQDF 303 Query: 306 VALRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKRR 364 +ALRI+E+AKEHG+ITMENKPLARAL+QR+EIGD +P DLFQAVAEVLAYVYKL GK + Sbjct: 304 MALRIKEIAKEHGIITMENKPLARALYQRSEIGDSIPGDLFQAVAEVLAYVYKLKGKAK 362 >B1DLM8.1 B1DLM8_9BACL Flagellar biosynthetic protein FlhB Length = 365 Score = 392 bits (1008), Expect = e-107, Method: Compositional matrix adjust. Identities = 197/356 (55%), Positives = 254/356 (71%) Query: 9 LNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERVVK 68 L+LQ F +KTEKATPKK QEARKKGQ+AK G + K R++ Sbjct: 8 LDLQLFSQEKTEKATPKKTQEARKKGQIAKSNDLPSSMILLFTFATFAALGSYYKTRIMS 67 Query: 69 LYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMVTE 128 ++ ++F N + M VT N+ +F + +L+ LAP+F I ++A++ N+ + GF++ E Sbjct: 68 VFQELFDNWLTMDVTTANIMPLFDKLAVDMLMFLAPIFAIVVIVAILGNVVQFGFLLTGE 127 Query: 129 GLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHVSA 188 L PKFSK+NPI GFK IFSMR+ VEFLKSI KLLIIG LVY +W + + L+ +S Sbjct: 128 SLKPKFSKLNPINGFKQIFSMRTVVEFLKSILKLLIIGILVYRAIWSDWDKILVLSSLSV 187 Query: 189 EGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGDPI 248 + + F A +T+ LG++ GAVL+I+A DY+YQ+YEH KSL+MSKQDIKDEYKK EGDP+ Sbjct: 188 QQIFSFTAGLTVKLGLETGAVLVILAFADYMYQRYEHAKSLRMSKQDIKDEYKKSEGDPL 247 Query: 249 IKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFVAL 308 IKGKIRE EVP ADV+ITNPTHFA+AL+YD S+MEAP IIAKG D +AL Sbjct: 248 IKGKIRERQRRMAMQRMMQEVPKADVVITNPTHFAIALQYDASKMEAPLIIAKGMDHLAL 307 Query: 309 RIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGKRR 364 RIRE+AKEHGV+ MENKPLARAL++R +IGD +PADLFQAVAEVLA VYK+ G+ R Sbjct: 308 RIREVAKEHGVVMMENKPLARALYERADIGDSIPADLFQAVAEVLAAVYKMKGRVR 363 >C0ZF82.1 C0ZF82_BREBN Flagellar biosynthetic protein FlhB Length = 365 Score = 345 bits (885), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 174/356 (48%), Positives = 230/356 (64%) Query: 7 YALNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKERV 66 Y ++LQ F G+KTEKATPKK+Q+ARKKGQVAK MM G + Sbjct: 8 YQVDLQFFNGEKTEKATPKKKQDARKKGQVAKSQDLSPALSLTAFFFLLMMLGSTMLGTF 67 Query: 67 VKLYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMMV 126 L + Q+ EN+ M + + ++ P+ ++F++A VN +VG+ Sbjct: 68 QDLMRESLITYTTWQLNEENLKVMVMQLVFEAIKIVGPILGLSFLVAFAVNYMQVGWHFS 127 Query: 127 TEGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAHV 186 TE L K KI+PIKG K IFS+RS VE LKS+ K+ Y+ Y LW KE L+ + Sbjct: 128 TEPLQMKLEKIDPIKGAKRIFSLRSLVELLKSLLKISAGIYVAYVILWNAKEQVVQLSLL 187 Query: 187 SAEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEGD 246 S F A+ LGI +G +L I+AV DY YQ+YEHEK+L+MSKQD+KDE+K+ EGD Sbjct: 188 SLGAVLSFTAEEVTKLGIYLGLLLFILAVLDYAYQRYEHEKNLRMSKQDLKDEHKQAEGD 247 Query: 247 PIIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDFV 306 P+IKGKIRE E+P ADVIITNPTHFAVA++YD S M AP +IAKGQD++ Sbjct: 248 PLIKGKIRERQRSMAIRRMMQELPKADVIITNPTHFAVAVRYDASAMSAPTVIAKGQDYL 307 Query: 307 ALRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 AL+IRE+AK+H ++TMENKPLARAL+ + E+G +P ++F+AVAEVLAYVYKL GK Sbjct: 308 ALKIREVAKKHRIVTMENKPLARALYSQVEVGQQIPEEMFKAVAEVLAYVYKLQGK 363 >B4ADY7.1 B4ADY7_BACPU Flagellar biosynthetic protein FlhB Length = 362 Score = 340 bits (872), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 165/357 (46%), Positives = 234/357 (65%) Query: 6 RYALNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKER 65 R L+LQ F G+KTEKATPKKRQ++RKKGQVAK G F+KER Sbjct: 4 RLRLDLQFFAGEKTEKATPKKRQDSRKKGQVAKSTDVNTAISFLLIFLSFFFIGPFMKER 63 Query: 66 VVKLYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMM 125 ++ L + M+M+V+ N+ ++F + ILL P+ + + ++ N +VGF+ Sbjct: 64 IIALIERFYSTTMLMKVSTSNIHQLFVQLLQETGILLMPILGVGMLAGIISNYLQVGFLF 123 Query: 126 VTEGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAH 185 TE + PK K++PIKGFK I+S+R+ VE LKSI K+ +IG+ + LW + L Sbjct: 124 STEVIKPKLEKLDPIKGFKRIYSIRALVELLKSILKIGLIGFATFIALWMHFDEILRLPL 183 Query: 186 VSAEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEG 245 ++AE F A MT+ +G+ L+I+A DY+YQK+++EK+L+MSKQDIKDEYKK EG Sbjct: 184 LTAEETLHFVANMTLIMGLYAAGALMILAGLDYMYQKFDYEKNLRMSKQDIKDEYKKSEG 243 Query: 246 DPIIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDF 305 DP+IK KI++ EVP ADVIITNPTH+A+ALKYD +M+AP ++AKG D Sbjct: 244 DPLIKSKIKQRQREMAMRRMMQEVPKADVIITNPTHYAIALKYDEEKMDAPFVVAKGTDI 303 Query: 306 VALRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 +AL+IR +AKEH ++T+EN+PLARAL+ + +I VP + F+A+AE+LAYVYK K Sbjct: 304 LALKIRTIAKEHDIMTVENRPLARALYDQVDIDQAVPEEYFKAIAEILAYVYKTKQK 360 >A8FDA3.1 A8FDA3_BACP2 Flagellar biosynthesis protein FlhB Length = 362 Score = 338 bits (868), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 163/357 (45%), Positives = 234/357 (65%) Query: 6 RYALNLQHFGGDKTEKATPKKRQEARKKGQVAKXXXXXXXXXXXXXXXXXMMFGGFIKER 65 R L+LQ F G+KTEKATPKKRQ++RKKGQVAK G F+KER Sbjct: 4 RLRLDLQFFAGEKTEKATPKKRQDSRKKGQVAKSTDVNTAISFLLIFLSFFFIGPFMKER 63 Query: 66 VVKLYTDVFQNRMMMQVTPENVSEMFRHYGLQILILLAPLFIITFVMALVVNLAEVGFMM 125 ++ L + M+M+V+ N+ ++F + +LL P+ + + ++ N +VGF+ Sbjct: 64 IIALIERFYSTTMLMKVSTSNIHQLFVELLQETGLLLMPILGVGMLAGIISNYLQVGFLF 123 Query: 126 VTEGLTPKFSKINPIKGFKNIFSMRSFVEFLKSIFKLLIIGYLVYSTLWGEKESFASLAH 185 TE + PK K++PIKGFK I+S+R+ VE LKSI K+ +IG+ + LW + L Sbjct: 124 STEVIKPKLEKLDPIKGFKRIYSIRALVELLKSILKIGLIGFATFIALWMHFDEILRLPL 183 Query: 186 VSAEGAYRFAAKMTMNLGIKIGAVLLIMAVFDYIYQKYEHEKSLKMSKQDIKDEYKKMEG 245 ++AE F A MT+ +G+ L+++A DY+YQK+++EK+L+MSKQDIKDEYKK EG Sbjct: 184 LTAEETLHFVASMTLIMGLYAAGALMVLAGLDYMYQKFDYEKNLRMSKQDIKDEYKKSEG 243 Query: 246 DPIIKGKIREXXXXXXXXXXXXEVPNADVIITNPTHFAVALKYDGSQMEAPQIIAKGQDF 305 DP+IK KI++ EVP ADVIITNPTH+A+ALKYD +M+AP ++AKG D Sbjct: 244 DPLIKSKIKQRQREMAMRRMMQEVPKADVIITNPTHYAIALKYDEEKMDAPFVVAKGTDI 303 Query: 306 VALRIRELAKEHGVITMENKPLARALFQRTEIGDVVPADLFQAVAEVLAYVYKLNGK 362 +AL+IR +AKEH ++T+EN+PLARAL+ + +I VP + F+A+AE+LAYVYK K Sbjct: 304 LALKIRTIAKEHDIMTVENRPLARALYDQVDIDQAVPEEYFKAIAEILAYVYKTKQK 360 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.138 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,775,392,904 Number of extensions: 62434258 Number of successful extensions: 196536 Number of sequences better than 1.0e-05: 1449 Number of HSP's gapped: 194552 Number of HSP's successfully gapped: 1449 Length of query: 364 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 227 Effective length of database: 1,770,017,148 Effective search space: 401793892596 Effective search space used: 401793892596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)