BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0042.p (533 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE1.1 B3KIE1_9BACI Flagellar biosynthesis protein FlhA 607 e-171 B1DLM9.1 B1DLM9_9BACL Flagellar biosynthesis protein FlhA 588 e-166 Q9KA52.1 Q9KA52_BACHD Flagella-associated protein 519 e-145 Q2B7H3.1 Q2B7H3_9BACI Flagellar biosynthesis protein A 510 e-142 B7GGA1.1 B7GGA1_ANOFW Flagellar biosynthesis pathway, component ... 506 e-141 >B3KIE1.1 B3KIE1_9BACI Flagellar biosynthesis protein FlhA Length = 677 Score = 607 bits (1564), Expect = e-171, Method: Compositional matrix adjust. Identities = 316/500 (63%), Positives = 351/500 (70%) Query: 4 LKARDLTXXXXXXXXXXXXXXPIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPS 63 +K +DL+ PIP WLLDV VAMNTK+ L FSIFPS Sbjct: 1 MKIKDLSVLAGVIGIVLMMILPIPIWLLDVLLIINISLALIILLVAMNTKEALDFSIFPS 60 Query: 64 XXXXXXXXXXXXNISTTKLILSDGHAGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXX 123 NISTTKLILS+ +AGEVVATFG WIA G+IA Sbjct: 61 LLLITTLFRVALNISTTKLILSEANAGEVVATFGKWIANGEIAVGFIVFLILVVVQFIVI 120 Query: 124 TKGSERVAEVGARFTLDAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGA 183 TKGSERVAEV ARFTLDAMPGKQMSIDADLNAG+INEQQA ERR KIEREADF+G+MDGA Sbjct: 121 TKGSERVAEVAARFTLDAMPGKQMSIDADLNAGLINEQQARERRSKIEREADFYGAMDGA 180 Query: 184 SKFVKGDAIASXXXXXXXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLIS 243 SKFVKGDAIA+ +S+HGMSF A+STYS+LTIGDGLVSQIPALLIS Sbjct: 181 SKFVKGDAIATIVIMLINLIGGFIIGISIHGMSFGDAMSTYSILTIGDGLVSQIPALLIS 240 Query: 244 TAAGLIVTRAASDGNLAEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXX 303 TAAGLIVTRA+S+GNLAEDLTGQL SYP LLYIVA T+ LG FTPI +++T P Sbjct: 241 TAAGLIVTRASSEGNLAEDLTGQLFSYPHLLYIVAGTVLLLGIFTPIHLITTAPIALVLF 300 Query: 304 XXXXTMSQKASRQQIAXXXXXXXXXXXXXRSPESVINLLSVDAIEFEFGYGLIPLADTGQ 363 TM R+Q+ RSPESVINLL VD IEFEFGYGLIPLAD Q Sbjct: 301 IAARTMQNNLDRKQVEVEQLEEEQQIEEVRSPESVINLLQVDPIEFEFGYGLIPLADAQQ 360 Query: 364 GGDLLDRIIMIRRQCALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLA 423 GGDLLDRIIMIRRQCALE+GLVVPVIRIRDNIQLKPNEYVIKIK HYLA Sbjct: 361 GGDLLDRIIMIRRQCALELGLVVPVIRIRDNIQLKPNEYVIKIKGNAVGGGELLLNHYLA 420 Query: 424 MSPGYEDESIHGIETVEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRY 483 MSPGYED+S+ GIET+EP+FGLPALWIDE++KERA+L+GYTVVDPPSVVATHLTELIKR+ Sbjct: 421 MSPGYEDDSVTGIETMEPAFGLPALWIDEAMKERADLAGYTVVDPPSVVATHLTELIKRH 480 Query: 484 GHELLGRXXXXGNQATCRQS 503 HEL+GR R+S Sbjct: 481 AHELIGRQETKALVDNIRES 500 >B1DLM9.1 B1DLM9_9BACL Flagellar biosynthesis protein FlhA Length = 677 Score = 588 bits (1516), Expect = e-166, Method: Compositional matrix adjust. Identities = 298/443 (67%), Positives = 328/443 (74%) Query: 48 VAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLILSDGHAGEVVATFGSWIARGQIAX 107 +AMNTK+ L FSIFP+ N+STT+ ILS GHAGEVV TFGSW+ GQIA Sbjct: 45 IAMNTKEALDFSIFPAILLVTTLFRLALNVSTTRNILSHGHAGEVVQTFGSWVGGGQIAI 104 Query: 108 XXXXXXXXXXXXXXXXTKGSERVAEVGARFTLDAMPGKQMSIDADLNAGMINEQQAGERR 167 TKGSERVAEV ARFTLDAMPGKQMSIDADLNAG+INEQQA ERR Sbjct: 105 GFVVFLILVVVQFIVITKGSERVAEVAARFTLDAMPGKQMSIDADLNAGLINEQQARERR 164 Query: 168 RKIEREADFFGSMDGASKFVKGDAIASXXXXXXXXXXXXXXXMSVHGMSFQAALSTYSVL 227 KIEREADF+GSMDGASKFVKGDAIAS M++HG SF AL TYS+L Sbjct: 165 SKIEREADFYGSMDGASKFVKGDAIASIIILLINLIGGFIIGMAIHGDSFSDALQTYSIL 224 Query: 228 TIGDGLVSQIPALLISTAAGLIVTRAASDGNLAEDLTGQLLSYPKLLYIVAVTIAFLGFF 287 TIGDGLVSQIPALLISTAAGLIVTRAASDGN A+D+T QLL YPKLLYIVA TI LG F Sbjct: 225 TIGDGLVSQIPALLISTAAGLIVTRAASDGNFAQDVTTQLLKYPKLLYIVAGTITLLGVF 284 Query: 288 TPITVMSTLPXXXXXXXXXXTMSQKASRQQIAXXXXXXXXXXXXXRSPESVINLLSVDAI 347 TPI ++ T+P M Q ++Q RSPESVI+LL VD I Sbjct: 285 TPIGILRTVPFAALLLFGAWRMQQSIAKQMHQEELMEEEQQIEEVRSPESVISLLQVDPI 344 Query: 348 EFEFGYGLIPLADTGQGGDLLDRIIMIRRQCALEMGLVVPVIRIRDNIQLKPNEYVIKIK 407 EFEFGYGLIPLADT QGGDLLDRIIMIRRQCALE+GLVVPVIRIRDNIQL+PNEYVIKIK Sbjct: 345 EFEFGYGLIPLADTQQGGDLLDRIIMIRRQCALELGLVVPVIRIRDNIQLRPNEYVIKIK 404 Query: 408 XXXXXXXXXXXXHYLAMSPGYEDESIHGIETVEPSFGLPALWIDESVKERAELSGYTVVD 467 HYLAMSPG+EDES+ GIET EP+FGLPALWIDE++KERAE+SGYTVVD Sbjct: 405 GNIVARGELLLNHYLAMSPGFEDESVIGIETTEPAFGLPALWIDEAMKERAEISGYTVVD 464 Query: 468 PPSVVATHLTELIKRYGHELLGR 490 PPSVVATHLTE+IKR+ HEL+GR Sbjct: 465 PPSVVATHLTEIIKRHAHELIGR 487 >Q9KA52.1 Q9KA52_BACHD Flagella-associated protein Length = 677 Score = 519 bits (1337), Expect = e-145, Method: Compositional matrix adjust. Identities = 268/487 (55%), Positives = 325/487 (66%) Query: 4 LKARDLTXXXXXXXXXXXXXXPIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPS 63 ++ARDL P+P +LD VAMNTK+PLQFSIFP+ Sbjct: 1 MRARDLAVLLSVILIVIMLIIPLPPAMLDFLIITNISLAILIILVAMNTKEPLQFSIFPT 60 Query: 64 XXXXXXXXXXXXNISTTKLILSDGHAGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXX 123 N+STT+ IL +G AG V+ TFG+++ G Sbjct: 61 LLLLVTLFRLGLNVSTTRSILGNGEAGNVIDTFGNFVIGGNPLVGFVVFLILIIIQFIVI 120 Query: 124 TKGSERVAEVGARFTLDAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGA 183 TKG+ERV+EVGARFTLDAMPGKQMSIDADLNAGMI++ +A ERR KIE+EADF+G+MDGA Sbjct: 121 TKGAERVSEVGARFTLDAMPGKQMSIDADLNAGMISDIEAKERREKIEKEADFYGAMDGA 180 Query: 184 SKFVKGDAIASXXXXXXXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLIS 243 SKFVKGDAIA M GMS + + +++LT+GDGLVSQIPAL+IS Sbjct: 181 SKFVKGDAIAGIVIVIINIIFGLVIGMMQMGMSLSDSANHFTLLTVGDGLVSQIPALIIS 240 Query: 244 TAAGLIVTRAASDGNLAEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXX 303 TA G+IVTRAASDGNL +D+T QLL+YPK+LY+ + TI LG TPI+++ T Sbjct: 241 TATGIIVTRAASDGNLGQDITKQLLAYPKMLYVASGTILALGLLTPISLIVTSIIAGVLA 300 Query: 304 XXXXTMSQKASRQQIAXXXXXXXXXXXXXRSPESVINLLSVDAIEFEFGYGLIPLADTGQ 363 +SQ +Q +SPESV+NLL +D IEFEFGYGLIPLADT Q Sbjct: 301 FGGYHLSQGEQKQLAEEEKEEASDESEDLKSPESVVNLLQIDPIEFEFGYGLIPLADTNQ 360 Query: 364 GGDLLDRIIMIRRQCALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLA 423 GGDLLDR++MIRRQ ALE+G+VVPVIRIRDNIQL+PNEY IKIK HY+A Sbjct: 361 GGDLLDRVVMIRRQMALELGMVVPVIRIRDNIQLQPNEYTIKIKGNEVAKGELLLDHYMA 420 Query: 424 MSPGYEDESIHGIETVEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRY 483 MSPG EDES+ GIETVEP+FGLPALWI E +KE+AELSGYTVVDPPSVV+THLTE+IKR+ Sbjct: 421 MSPGVEDESVVGIETVEPAFGLPALWISEEMKEQAELSGYTVVDPPSVVSTHLTEVIKRH 480 Query: 484 GHELLGR 490 HEL+GR Sbjct: 481 AHELIGR 487 >Q2B7H3.1 Q2B7H3_9BACI Flagellar biosynthesis protein A Length = 678 Score = 510 bits (1313), Expect = e-142, Method: Compositional matrix adjust. Identities = 256/466 (54%), Positives = 311/466 (66%) Query: 25 PIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPSXXXXXXXXXXXXNISTTKLIL 84 P P+WLL + ++MN +PLQFSIFPS N+STT+ IL Sbjct: 22 PFPSWLLSILIIMNISLALLVLLISMNMNEPLQFSIFPSLLLLLTLFRLGLNVSTTRSIL 81 Query: 85 SDGHAGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXXTKGSERVAEVGARFTLDAMPG 144 S G AG VV TFG+++ G + TKGSERV+EV ARFTLDAMPG Sbjct: 82 SKGEAGGVVETFGTFVVGGNVIVGMVVFLILIIIQFIVITKGSERVSEVAARFTLDAMPG 141 Query: 145 KQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGASKFVKGDAIASXXXXXXXXXX 204 KQMSIDADLNAGMI+E +A ERR K+ READF+GSMDGASKFVKGD+IA Sbjct: 142 KQMSIDADLNAGMISEHEARERREKVSREADFYGSMDGASKFVKGDSIAGIIIVLINLIF 201 Query: 205 XXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLISTAAGLIVTRAASDGNLAEDLT 264 M G+ F A S YS+LT+GDG+VSQIPALLISTA G++VTRAAS+GNL +D+T Sbjct: 202 GIVIGMMQQGLGFADAASRYSLLTVGDGIVSQIPALLISTATGIVVTRAASEGNLGKDIT 261 Query: 265 GQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXXXXXXTMSQKASRQQIAXXXXX 324 Q+L+YPK+LY+ TI LG FTPI+ + T P +S+ + Sbjct: 262 SQILAYPKMLYVTGATIFLLGLFTPISNILTFPIAGLFAFGGFMLSRVKEPDKEQLQEIE 321 Query: 325 XXXXXXXXRSPESVINLLSVDAIEFEFGYGLIPLADTGQGGDLLDRIIMIRRQCALEMGL 384 +SPESV++LL+VD IEFEFGYGLIPLAD QGGDLLDRI+MIRRQ A+E+GL Sbjct: 322 EELETDEMKSPESVVSLLNVDPIEFEFGYGLIPLADANQGGDLLDRIVMIRRQLAIELGL 381 Query: 385 VVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLAMSPGYEDESIHGIETVEPSFG 444 V+PV+RIRDNIQL+PNEY +KIK HYLAMSPG ED+SI GI+TVEPSFG Sbjct: 382 VIPVVRIRDNIQLQPNEYRLKIKGNEMARGELLLDHYLAMSPGIEDDSIDGIDTVEPSFG 441 Query: 445 LPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRYGHELLGR 490 LPA WI E KE+AE+ GYTVVDPPSVV+TH+TE+IK ++LLGR Sbjct: 442 LPAKWITEETKEQAEIFGYTVVDPPSVVSTHITEIIKANAYDLLGR 487 >B7GGA1.1 B7GGA1_ANOFW Flagellar biosynthesis pathway, component FlhA Length = 678 Score = 506 bits (1304), Expect = e-141, Method: Compositional matrix adjust. Identities = 260/487 (53%), Positives = 317/487 (65%) Query: 4 LKARDLTXXXXXXXXXXXXXXPIPAWLLDVXXXXXXXXXXXXXXVAMNTKDPLQFSIFPS 63 + ARDL+ P+P+WLL V +MN ++PLQFSIFPS Sbjct: 1 MSARDLSVLIMVVLIVAMLIIPLPSWLLSVLIIMNISLALLVLLTSMNMREPLQFSIFPS 60 Query: 64 XXXXXXXXXXXXNISTTKLILSDGHAGEVVATFGSWIARGQIAXXXXXXXXXXXXXXXXX 123 N+STT+ ILS G AG VV TFG+++ G + Sbjct: 61 LLLLLTLFRLGLNVSTTRSILSKGEAGGVVETFGTFVIGGNVVVGFVVFLILIIIQFVVI 120 Query: 124 TKGSERVAEVGARFTLDAMPGKQMSIDADLNAGMINEQQAGERRRKIEREADFFGSMDGA 183 TKG+ERV+EV ARFTLDAMPGKQMSIDADLNAG+I+EQQA ERR KI +EADF+G+MDGA Sbjct: 121 TKGAERVSEVAARFTLDAMPGKQMSIDADLNAGIISEQQARERREKIAQEADFYGAMDGA 180 Query: 184 SKFVKGDAIASXXXXXXXXXXXXXXXMSVHGMSFQAALSTYSVLTIGDGLVSQIPALLIS 243 SKFVKGDAIA M GM A Y++LT+GDG+VSQIPALLIS Sbjct: 181 SKFVKGDAIAGIIIVLINMLFGIVIGMVQQGMDIGEAARRYTLLTVGDGIVSQIPALLIS 240 Query: 244 TAAGLIVTRAASDGNLAEDLTGQLLSYPKLLYIVAVTIAFLGFFTPITVMSTLPXXXXXX 303 TA G++VTRAASD NL D+ QL +YPK+LY+ A TI LG FTPI + T+P Sbjct: 241 TATGIVVTRAASDDNLGADIMRQLFAYPKMLYVTAGTIFLLGLFTPINDLLTIPIASLLA 300 Query: 304 XXXXTMSQKASRQQIAXXXXXXXXXXXXXRSPESVINLLSVDAIEFEFGYGLIPLADTGQ 363 +++A + +SPESV++LLSVD IEFEFGYGLIPLAD Q Sbjct: 301 FGAYQFTKQADAPETTTEETEEEIEMDQMKSPESVVSLLSVDPIEFEFGYGLIPLADANQ 360 Query: 364 GGDLLDRIIMIRRQCALEMGLVVPVIRIRDNIQLKPNEYVIKIKXXXXXXXXXXXXHYLA 423 GGDLLDRI+MIRRQ ALE+G+V+PV+RIRDNIQL+PNEY +KIK HYLA Sbjct: 361 GGDLLDRIVMIRRQLALELGIVIPVVRIRDNIQLQPNEYRLKIKGNEVARGELLLDHYLA 420 Query: 424 MSPGYEDESIHGIETVEPSFGLPALWIDESVKERAELSGYTVVDPPSVVATHLTELIKRY 483 MSPG +D++I GI+TVEP+FGLPA WI E +KERAE+ GYTVVDPPSVV+TH+TEL+K + Sbjct: 421 MSPGIDDDAIEGIDTVEPAFGLPAKWISEEMKERAEMFGYTVVDPPSVVSTHITELLKAH 480 Query: 484 GHELLGR 490 HELLGR Sbjct: 481 AHELLGR 487 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,318,669,202 Number of extensions: 75330419 Number of successful extensions: 150524 Number of sequences better than 1.0e-05: 1454 Number of HSP's gapped: 146983 Number of HSP's successfully gapped: 1742 Length of query: 533 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 392 Effective length of database: 1,732,333,088 Effective search space: 679074570496 Effective search space used: 679074570496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)