BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0040.p (479 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE2.1 B3KIE2_9BACI GTP-binding signal recognition particle SR... 374 e-101 C0ZF80.1 C0ZF80_BREBN Probable flagellar biosynthesis protein FlhF 280 3e-73 C5D9A5.1 C5D9A5_9BACI GTP-binding signal recognition particle SR... 227 2e-57 B1DLN0.1 B1DLN0_9BACL GTP-binding signal recognition particle SR... 226 3e-57 Q3V7T7.1 Q3V7T7_GEOKA Flagellar biosynthetic protein (Flagellar ... 226 4e-57 >B3KIE2.1 B3KIE2_9BACI GTP-binding signal recognition particle SRP54 G-domain Length = 479 Score = 374 bits (960), Expect = e-101, Method: Compositional matrix adjust. Identities = 180/290 (62%), Positives = 226/290 (77%) Query: 186 SELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWISNIF 245 SE E VL E+++MK+W+ R++R + A LPE L IRD+L++Q+ A L +EW++ Sbjct: 186 SESEQRVLEELKEMKEWIARLSRQNLEAQHLPEPLHVIRDQLLKQDLSAKLAEEWLAAAE 245 Query: 246 DQWHEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIAKLA 305 D W + E + Q E FL GRI +G+ P TRIVY+AGPTGVGKTTTIAK+A Sbjct: 246 DAWKASQMQMSETELLNVIYKQAEAFLEGRIGDGVQPGTRIVYVAGPTGVGKTTTIAKIA 305 Query: 306 AEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESCDLV 365 AEQLFR RKVG ITSDTYRISAVEQLRTYA+ILN+P+EVVQSPGD+QRAL +L+ CDLV Sbjct: 306 AEQLFRNQRKVGFITSDTYRISAVEQLRTYASILNVPMEVVQSPGDMQRALQKLQDCDLV 365 Query: 366 LMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDKVIF 425 LMDTAGRNYRNE+LV+ELQSL + +SET+LVLSLTSKS DM +I +HF +Y+LDKVIF Sbjct: 366 LMDTAGRNYRNELLVSELQSLFSHSDQSETYLVLSLTSKSSDMIEIADHFSKYELDKVIF 425 Query: 426 TKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 TK+DETGSYG LFN+++ L+LSY+TNGQNVPDDLL A ++L ++LG Sbjct: 426 TKMDETGSYGALFNLIDAHNLRLSYMTNGQNVPDDLLMADPERLCHLLLG 475 Score = 80.5 bits (197), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 41/75 (54%), Positives = 52/75 (69%), Gaps = 1/75 (1%) Query: 1 MRVKRYVVDTMPDAMHSIRSELGSDAVILSTKEVRVXXXXXXXXXXXIEVVAAVEDAQKP 60 MRVKRY+VDTMP+AM IR++LG+DAVILSTKE +V IEV+AAVE A+KP Sbjct: 1 MRVKRYIVDTMPEAMQHIRTDLGADAVILSTKETKVGGFLGMFTRKKIEVIAAVEQAEKP 60 Query: 61 -GASAKTPAALLNIP 74 G+S P ++P Sbjct: 61 KGSSPVKPKVTPSVP 75 >C0ZF80.1 C0ZF80_BREBN Probable flagellar biosynthesis protein FlhF Length = 448 Score = 280 bits (716), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 174/479 (36%), Positives = 262/479 (54%), Gaps = 36/479 (7%) Query: 1 MRVKRYVVDTMPDAMHSIRSELGSDAVILSTKEVRVXXXXXXXXXXXIEVVAAVEDAQKP 60 MRVKRY+VD+MP+AM IR++LG DAVIL++K ++ IEV+AAV++ Sbjct: 1 MRVKRYIVDSMPEAMEKIRTDLGMDAVILNSKPIKTGGLFGMFGKQRIEVIAAVDEKASD 60 Query: 61 GASAKTPAALLNIPRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSSGSEAAAKQTFAEI 120 ++ A S+G E ++ Sbjct: 61 RENSTVAAEHRTNNVLPKSQTGTYTAAQAYRRPTVTKTSENQQTESTGRE--------QV 112 Query: 121 AAALSDTLENKGNVAVLPAVQEXXXXXYLEADEAPIQHSXXXXXXXXXXXXIDEARNKKL 180 AA++ T VA LP + + E QH+ + ++ + L Sbjct: 113 VAAMTQTA-----VATLPKEEITDTRAMPKQGEPTEQHAP-----------VTTSQERAL 156 Query: 181 NAASTSELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEW 240 A++ + + E+RDM+Q +++ + +LP ++ IR +L++QE L E Sbjct: 157 PASNHDAIAT----EMRDMRQMFQKLLVHDLSE-QLPPAVQSIRAQLLKQEVTEELTAEI 211 Query: 241 ISNIFDQWHEEGRIWTPEE-FNVR---MAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVG 296 I I ++ + G WT EE F V + + +E + + + + + R + GPTGVG Sbjct: 212 IRQIMEK-SQPGEKWTEEEAFTVCRTIITSMIEPYTAS--SPRLGKNVRYAFFFGPTGVG 268 Query: 297 KTTTIAKLAAEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRAL 356 KTTTIAKLAA + ++ RK+G IT+DTYR++AVEQL+TYA ILN+PLEVV SP ++ A+ Sbjct: 269 KTTTIAKLAANSMLKEKRKIGFITADTYRMAAVEQLKTYANILNVPLEVVFSPREMAAAM 328 Query: 357 FRLESCDLVLMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFG 416 RL CDL+ +DTAGRN+RN+ V ++ LL S +LVLSL+SK DMK IV++F Sbjct: 329 ERLSDCDLIFVDTAGRNFRNDEYVEGIRELLEHGKDSVNYLVLSLSSKFNDMKAIVQNFA 388 Query: 417 RYQLDKVIFTKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 Q+ +VIFTK DET S+G + NV + L+LSY+T GQNVPDD++ AT + +S MI+G Sbjct: 389 EVQVKQVIFTKADETNSFGTMLNVCQEMNLQLSYVTTGQNVPDDIVVATPELVSTMIMG 447 >C5D9A5.1 C5D9A5_9BACI GTP-binding signal recognition particle SRP54 G-domain protein Length = 372 Score = 227 bits (579), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 110/288 (38%), Positives = 186/288 (64%), Gaps = 1/288 (0%) Query: 189 ESDVLREIRDMKQWMERIA-RYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWISNIFDQ 247 ++++L E++++K +++++ ++G P+ L ++ RL+ QE A L E ++++ ++ Sbjct: 81 DAELLHELKELKTMLKQLSTNVTTGFSHYPKPLREVHYRLVRQEISAPLAQEIMADLLEK 140 Query: 248 WHEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIAKLAAE 307 W++ G ++ V++ LS GI+ + + + GPTGVGKTTT+AK+AA Sbjct: 141 WYKSGGKAAEKQVFAWAKEIVKEKLSPLPFGGISFQKKYINVVGPTGVGKTTTLAKIAAR 200 Query: 308 QLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESCDLVLM 367 + G+ + IT+DTYRI+A++QL+TYA ILN+PLEV + D A +L D+V + Sbjct: 201 CVLEHGKNIAFITTDTYRIAAIDQLKTYANILNVPLEVCYNLQDFHEAKKKLSDRDVVFI 260 Query: 368 DTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDKVIFTK 427 DTAGRN+R+ V +L++++ + ETFLV +LT+K DMK I E F +D+ IFTK Sbjct: 261 DTAGRNFRHLQYVKDLRNMIDFNEEMETFLVFALTAKYEDMKTIYEQFSLIPIDRFIFTK 320 Query: 428 LDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 LDET YG +FN++ ++ + +Y+TNGQNVPDD++ A+ +Q+ M+ G Sbjct: 321 LDETTHYGAMFNLMMEYKVGAAYLTNGQNVPDDMMEASVEQMMNMLFG 368 >B1DLN0.1 B1DLN0_9BACL GTP-binding signal recognition particle SRP54 G-domain Length = 416 Score = 226 bits (577), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 124/292 (42%), Positives = 186/292 (63%), Gaps = 1/292 (0%) Query: 184 STSELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWISN 243 + + + D+L EIR +KQ +++++ ++ P ++ + RL++QE + L+D I Sbjct: 121 TINRINDDLLEEIRYIKQSLKQLS-MQKPEIDRPLAIQSLYGRLVQQEVEPELIDRLIDA 179 Query: 244 IFDQWHEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIAK 303 + ++ + T E + ++L I+ RIV+ GPTGVGKTTTIAK Sbjct: 180 VNERIAGNKEMPTGAETWQTARELLLEWLDPYSHQSISESARIVHFVGPTGVGKTTTIAK 239 Query: 304 LAAEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESCD 363 LAA Q + GR++GLITSDTYRI+AV+QLRTYA ILN+P+EVV SP DL +A +LE + Sbjct: 240 LAAGQSIKFGRQIGLITSDTYRIAAVDQLRTYANILNVPMEVVFSPMDLPKAFKQLEDRE 299 Query: 364 LVLMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDKV 423 L+ MDTAGRN+RNE+ V+E+ SLL +SET LVLSLT K++DM + ++F +Y + KV Sbjct: 300 LIYMDTAGRNFRNELHVSEVNSLLQSSQQSETVLVLSLTGKTKDMMAVADNFVKYGVRKV 359 Query: 424 IFTKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 +FTKLDET YG + N+ L+ ++ +GQ VPDD+ ++ +LG Sbjct: 360 LFTKLDETSVYGSILNLALKNELQPVFVASGQTVPDDIAPFNAERYIDSLLG 411 Score = 65.5 bits (158), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 33/67 (49%), Positives = 43/67 (64%) Query: 1 MRVKRYVVDTMPDAMHSIRSELGSDAVILSTKEVRVXXXXXXXXXXXIEVVAAVEDAQKP 60 MRVKRYVV+ +P+A+ IRSELG DAVIL+TKEVR+ +EV+AA+E Sbjct: 1 MRVKRYVVNALPEALPMIRSELGKDAVILNTKEVRIGGFMGMFRKKKMEVIAAIESGAAA 60 Query: 61 GASAKTP 67 +A P Sbjct: 61 PVAATQP 67 >Q3V7T7.1 Q3V7T7_GEOKA Flagellar biosynthetic protein (Flagellar GTP-binding protein) Length = 409 Score = 226 bits (576), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 122/293 (41%), Positives = 175/293 (59%), Gaps = 1/293 (0%) Query: 183 ASTSELESDVLREIRDMKQWMERIARYSSGAVELPEQLEQIRDRLMEQETDAVLVDEWIS 242 A+ E + + REI ++K + + SS + P L + RL+ Q V V + + Sbjct: 114 AAEREEDGSLAREIEEVKALIRQFNARSSSLL-YPPPLAEAERRLVRQGMADVYVRQVMD 172 Query: 243 NIFDQWHEEGRIWTPEEFNVRMAAQVEDFLSGRIANGIAPDTRIVYIAGPTGVGKTTTIA 302 + ++W+ + +P + V D LS A + + + GPTGVGKTTT+A Sbjct: 173 RLLERWYADKDSRSPAAVSAWTKEAVRDLLSTLPFVKTAEQKKYMILLGPTGVGKTTTLA 232 Query: 303 KLAAEQLFRQGRKVGLITSDTYRISAVEQLRTYAAILNMPLEVVQSPGDLQRALFRLESC 362 K+A + QG+KVG IT+DTYRI+A++QL+TYA IL+ PLEV + D + A +L C Sbjct: 233 KMAGRAVLEQGKKVGFITADTYRIAAIDQLKTYADILHAPLEVCYNADDFRTAKRKLAGC 292 Query: 363 DLVLMDTAGRNYRNEMLVAELQSLLAKELKSETFLVLSLTSKSRDMKKIVEHFGRYQLDK 422 DLV +DTAGRN+RN V ELQ L + ++ETFLV + T K DMK I HF R +D+ Sbjct: 293 DLVFVDTAGRNFRNPQYVDELQQTLEFDSETETFLVFAATGKYDDMKMIYNHFSRLPIDR 352 Query: 423 VIFTKLDETGSYGPLFNVLNDFPLKLSYITNGQNVPDDLLAATKDQLSGMILG 475 +I TKLDET S+G + NVL D L +Y TNGQNVPDD++ A+ D+L ++ G Sbjct: 353 LIVTKLDETDSFGTIVNVLLDSRLAAAYFTNGQNVPDDMVEASADRLVHLLFG 405 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,287,129,674 Number of extensions: 82053270 Number of successful extensions: 382775 Number of sequences better than 1.0e-05: 3659 Number of HSP's gapped: 377203 Number of HSP's successfully gapped: 3662 Length of query: 479 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 339 Effective length of database: 1,741,754,103 Effective search space: 590454640917 Effective search space used: 590454640917 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)