BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0038.p (458 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE4.1 B3KIE4_9BACI Protein-glutamate methylesterase EC=3.1.1.61 318 6e-85 B1DLN2.1 B1DLN2_9BACL Response regulator receiver modulated CheB... 246 3e-63 C5D9A7.1 C5D9A7_9BACI Response regulator receiver modulated CheB... 201 1e-49 B1HQY0.1 B1HQY0_LYSSC Chemotaxis response regulator protein-glut... 201 2e-49 A3IF07.1 A3IF07_9BACI Chemotaxis-specific methylesterase EC=3.1.... 201 2e-49 >B3KIE4.1 B3KIE4_9BACI Protein-glutamate methylesterase EC=3.1.1.61 Length = 444 Score = 318 bits (816), Expect = 6e-85, Method: Compositional matrix adjust. Identities = 150/190 (78%), Positives = 164/190 (86%) Query: 268 LRKLVAVGCSTGGPRALKAFLENIPADFPAPIVIVQHMPPNFTKSLAQRLNTFSPLEVAE 327 L+ LVAVGCSTGGPRALKAFLE IP DF APIVIVQHMPPNFTKSLAQRLN+FS LEV E Sbjct: 251 LKDLVAVGCSTGGPRALKAFLEQIPGDFHAPIVIVQHMPPNFTKSLAQRLNSFSQLEVME 310 Query: 328 AEQGMILRQGAAYIAPGGYHMRVVPAAGGQYAIDLTLEEARNGHRPSVDTLFESILPYTS 387 AE GM+L G AYIAPGG HMRV GG Y I ++ +E RNGHRPSVD LFES+LP TS Sbjct: 311 AEHGMVLEPGKAYIAPGGSHMRVHRPEGGAYQIRISQDETRNGHRPSVDVLFESLLPLTS 370 Query: 388 LERHAVIMTGMGSDGARMMKSLYDSGVTSTFAESEETCVVYGMPRSAVELQCVRHVLPLQ 447 ++RHAV+MTGMGSDGARMMK LYD+GVTSTFAESE+TCVVYGMPRSAVEL CV HVLPL Sbjct: 371 VKRHAVLMTGMGSDGARMMKELYDAGVTSTFAESEQTCVVYGMPRSAVELGCVSHVLPLH 430 Query: 448 EIAPRLVKAV 457 EIAPR+V+AV Sbjct: 431 EIAPRMVQAV 440 Score = 181 bits (458), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 89/131 (67%), Positives = 106/131 (80%) Query: 1 LVVDDSAFMRKIISDLIENDANFQVAATASNGREAIEKVNELSPDLVTMDVEMPEMNGLE 60 LVVDDSAFMRKI SD IE D +FQV TA NG +A+EKV EL+PD++T+DVEMPEMNGL+ Sbjct: 7 LVVDDSAFMRKIFSDFIERDKSFQVVGTAENGLDAVEKVKELNPDVITLDVEMPEMNGLD 66 Query: 61 ALKIIMAQRPLPVIMLSGINEEGMKETILALEWGAFDFIRKPSISNSQDIIAVGVSLREQ 120 ALK+IM PVIMLSG+NE+GM+ETILALE GAFDFIRKPSISN+ DI V L +Q Sbjct: 67 ALKLIMDAGSRPVIMLSGLNEQGMRETILALEAGAFDFIRKPSISNALDIEQVRSELMKQ 126 Query: 121 MKEAMLARQQR 131 + AM A+Q+R Sbjct: 127 LNAAMQAKQRR 137 >B1DLN2.1 B1DLN2_9BACL Response regulator receiver modulated CheB methylesterase EC=3.1.1.61 Length = 442 Score = 246 bits (629), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 118/197 (59%), Positives = 146/197 (74%), Gaps = 1/197 (0%) Query: 261 RKSANKGLRKLVAVGCSTGGPRALKAFLENIPADFPAPIVIVQHMPPNFTKSLAQRLNTF 320 +K+A+ +L+A+G STGGPRAL + ++PA AP+++VQHMPP FTKSLAQRL++F Sbjct: 244 QKTASDSFDQLIAIGTSTGGPRALHEVITSLPARLAAPVLVVQHMPPKFTKSLAQRLDSF 303 Query: 321 SPLEVAEAEQGMILRQGAAYIAPGGYHMRVVPAAGGQYAIDLTLEEARNGHRPSVDTLFE 380 S LEV EA QG L+ G YIAPGG+HM + G Y + LT + NGHRPSVD LFE Sbjct: 304 SELEVVEASQGERLKAGVVYIAPGGHHMELAKDQSGYY-VQLTQKPPCNGHRPSVDVLFE 362 Query: 381 SILPYTSLERHAVIMTGMGSDGARMMKSLYDSGVTSTFAESEETCVVYGMPRSAVELQCV 440 S+ P+ L+RHAVIMTGMGSDGA+ MK+L DSG S AESE+TCVVYGMPRSA+E CV Sbjct: 363 SLTPFDELKRHAVIMTGMGSDGAKGMKALVDSGAESAIAESEQTCVVYGMPRSAIETGCV 422 Query: 441 RHVLPLQEIAPRLVKAV 457 + VLPLQ+IA L AV Sbjct: 423 KTVLPLQQIAAELQHAV 439 Score = 155 bits (393), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 78/129 (60%), Positives = 97/129 (75%) Query: 1 LVVDDSAFMRKIISDLIENDANFQVAATASNGREAIEKVNELSPDLVTMDVEMPEMNGLE 60 LVVDDS FMRKI SDLI D F + ATA NG EAIE V + PD VT+D+EMPE+NGL+ Sbjct: 7 LVVDDSPFMRKIFSDLIVQDPQFTIVATACNGLEAIEAVKKWKPDAVTLDLEMPELNGLD 66 Query: 61 ALKIIMAQRPLPVIMLSGINEEGMKETILALEWGAFDFIRKPSISNSQDIIAVGVSLREQ 120 AL+ IM ++P PVIMLSGI+EE +ETI AL+ GAFDFIRKP + S DI+ VG L E+ Sbjct: 67 ALQRIMIEQPTPVIMLSGISEENTRETIKALQLGAFDFIRKPMGAFSNDIVRVGELLLEK 126 Query: 121 MKEAMLARQ 129 ++ A+L R+ Sbjct: 127 LRIAVLTRR 135 >C5D9A7.1 C5D9A7_9BACI Response regulator receiver modulated CheB methylesterase EC=3.1.1.61 Length = 365 Score = 201 bits (512), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 101/192 (52%), Positives = 134/192 (69%), Gaps = 5/192 (2%) Query: 269 RKLVAVGCSTGGPRALKAFLENIPADFPAPIVIVQHMPPNFTKSLAQRLNTFSPLEVAEA 328 +K+V +G STGGPRAL+ L +P + API+IVQHMP FTKSLAQRL++ S + V EA Sbjct: 160 KKIVCIGTSTGGPRALQQVLTRLPENIAAPIMIVQHMPKGFTKSLAQRLDSLSQITVKEA 219 Query: 329 EQGMILRQGAAYIAPGGYHMRVVPAAGGQYAIDLTLEEA--RNGHRPSVDTLFESILPYT 386 E G L+ G AYIAPGG+H+ V + G A +E++ RNGHRPSVD LFESI T Sbjct: 220 EDGETLQNGTAYIAPGGFHLLVRESNGSLTA---CVEQSPPRNGHRPSVDVLFESISALT 276 Query: 387 SLERHAVIMTGMGSDGARMMKSLYDSGVTSTFAESEETCVVYGMPRSAVELQCVRHVLPL 446 E+ AVIMTGMGSDG +K L ++G T AE++ET VV+GMPR+A++ V ++PL Sbjct: 277 GYEKIAVIMTGMGSDGTAGLKKLKENGNTKVIAEAQETAVVFGMPRAAIQANVVDSIVPL 336 Query: 447 QEIAPRLVKAVK 458 ++IA +VK V+ Sbjct: 337 EQIAEMIVKYVE 348 Score = 132 bits (332), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 63/113 (55%), Positives = 84/113 (74%) Query: 1 LVVDDSAFMRKIISDLIENDANFQVAATASNGREAIEKVNELSPDLVTMDVEMPEMNGLE 60 LVVDDSAFMRK I+DL+ + +V A NG+EA++KV+ L+PD+VT+DVEMP MNG++ Sbjct: 7 LVVDDSAFMRKFITDLLSENPQIEVVGAARNGKEALKKVSLLNPDVVTLDVEMPIMNGID 66 Query: 61 ALKIIMAQRPLPVIMLSGINEEGMKETILALEWGAFDFIRKPSISNSQDIIAV 113 ALK IM + PLPV+MLS EG K TI+A+++GA DF+ KPS S D+ V Sbjct: 67 ALKQIMKEHPLPVVMLSSTTTEGAKNTIIAMQYGAIDFVAKPSGEISLDLYKV 119 >B1HQY0.1 B1HQY0_LYSSC Chemotaxis response regulator protein-glutamate methylesterase EC=3.1.1.61 Length = 383 Score = 201 bits (510), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 100/192 (52%), Positives = 130/192 (67%), Gaps = 2/192 (1%) Query: 265 NKGLRKLVAVGCSTGGPRALKAFLENIPADFPAPIVIVQHMPPNFTKSLAQRLNTFSPLE 324 NK +K+V +G STGGPRAL+ + IP API+IVQHMP FTKSLA RL+ S + Sbjct: 190 NKAGKKIVLIGTSTGGPRALQEVITKIPKSIQAPILIVQHMPAGFTKSLATRLDQLSEVV 249 Query: 325 VAEAEQGMILRQGAAYIAPGGYHMRVVPAAGGQYAIDLTLEEA-RNGHRPSVDTLFESIL 383 V EAEQG IL+ G AYIAPGGYH++ + G + I L +EA R+GHRPSVD +FE + Sbjct: 250 VKEAEQGDILQNGVAYIAPGGYHLK-LRKVGTTFGIVLDNQEAPRSGHRPSVDVMFEDVS 308 Query: 384 PYTSLERHAVIMTGMGSDGARMMKSLYDSGVTSTFAESEETCVVYGMPRSAVELQCVRHV 443 Y ++ AVIMTGMG DG+ +K+L +G AES ETC+VYGMP++AVE Q V V Sbjct: 309 QYKDFDKVAVIMTGMGHDGSNGLKALKKTGNVIAIAESAETCIVYGMPKAAVETQLVDEV 368 Query: 444 LPLQEIAPRLVK 455 + +IA ++K Sbjct: 369 ADVDDIALTIMK 380 Score = 129 bits (324), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 64/126 (50%), Positives = 88/126 (69%) Query: 1 LVVDDSAFMRKIISDLIENDANFQVAATASNGREAIEKVNELSPDLVTMDVEMPEMNGLE 60 LVVDDSAFMRK+ISD ++ +V TA NG++AI+K+ L P +VTMD+EMPEMNGL+ Sbjct: 10 LVVDDSAFMRKLISDFFVGNSKVEVVGTARNGKDAIKKIQSLKPSVVTMDIEMPEMNGLD 69 Query: 61 ALKIIMAQRPLPVIMLSGINEEGMKETILALEWGAFDFIRKPSISNSQDIIAVGVSLREQ 120 ALK IM Q P+PVIMLS + G + I A+E GA DF+ KPS + S D+ + L + Sbjct: 70 ALKEIMIQCPVPVIMLSSTTQLGTENAIAAIESGAVDFVAKPSGTISLDLHKIQSELVHK 129 Query: 121 MKEAML 126 +++A + Sbjct: 130 VEQAAM 135 >A3IF07.1 A3IF07_9BACI Chemotaxis-specific methylesterase EC=3.1.1.61 Length = 384 Score = 201 bits (510), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 99/192 (51%), Positives = 130/192 (67%), Gaps = 2/192 (1%) Query: 265 NKGLRKLVAVGCSTGGPRALKAFLENIPADFPAPIVIVQHMPPNFTKSLAQRLNTFSPLE 324 NK +K+V +G STGGPRAL+ + IP API+IVQHMP FTKSLA RL+ S + Sbjct: 191 NKAGKKIVLIGTSTGGPRALQEVITKIPKSIQAPILIVQHMPAGFTKSLATRLDQLSEIV 250 Query: 325 VAEAEQGMILRQGAAYIAPGGYHMRVVPAAGGQYAIDLTLEEA-RNGHRPSVDTLFESIL 383 V EAEQG IL+ G AYIAPGGYH++ + G + I L +EA R+GHRPSVD +FE + Sbjct: 251 VKEAEQGDILQNGVAYIAPGGYHLK-LRKVGTTFGIVLDNQEAPRSGHRPSVDVMFEDVS 309 Query: 384 PYTSLERHAVIMTGMGSDGARMMKSLYDSGVTSTFAESEETCVVYGMPRSAVELQCVRHV 443 Y ++ AVIMTGMG DG+ +K+L +G AES ETC+VYGMP++AVE Q + V Sbjct: 310 QYKDFDKVAVIMTGMGHDGSNGLKALKKTGNVMAIAESAETCIVYGMPKAAVETQLIDEV 369 Query: 444 LPLQEIAPRLVK 455 + +IA ++K Sbjct: 370 ADVDDIALTIMK 381 Score = 130 bits (326), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 64/126 (50%), Positives = 88/126 (69%) Query: 1 LVVDDSAFMRKIISDLIENDANFQVAATASNGREAIEKVNELSPDLVTMDVEMPEMNGLE 60 LVVDDSAFMRK+ISD ++ +V TA NG++AI+K+ L P +VTMD+EMPEMNGL+ Sbjct: 10 LVVDDSAFMRKLISDFFVGNSKVEVVGTARNGKDAIKKIQSLKPSVVTMDIEMPEMNGLD 69 Query: 61 ALKIIMAQRPLPVIMLSGINEEGMKETILALEWGAFDFIRKPSISNSQDIIAVGVSLREQ 120 ALK IM Q P+PVIMLS + G + I A+E GA DF+ KPS + S D+ + L + Sbjct: 70 ALKEIMIQCPVPVIMLSSTTQRGTENAIAAIESGAVDFVAKPSGTISLDLHKIQSELVHK 129 Query: 121 MKEAML 126 +++A + Sbjct: 130 VEQAAM 135 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,316,879,041 Number of extensions: 88345174 Number of successful extensions: 328324 Number of sequences better than 1.0e-05: 8124 Number of HSP's gapped: 338194 Number of HSP's successfully gapped: 9101 Length of query: 458 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 319 Effective length of database: 1,751,175,118 Effective search space: 558624862642 Effective search space used: 558624862642 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 133 (55.8 bits)