BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0036.p (153 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE6.1 B3KIE6_9BACI CheW domain protein 258 9e-68 B1DLN4.1 B1DLN4_9BACL CheW protein 238 1e-61 C0ZF76.1 C0ZF76_BREBN Chemotaxis protein CheW 187 3e-46 B2A372.1 B2A372_NATTJ CheW protein 171 2e-41 C3J423.1 C3J423_9BACI CheW protein 169 9e-41 >B3KIE6.1 B3KIE6_9BACI CheW domain protein Length = 153 Score = 258 bits (660), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 126/152 (82%), Positives = 145/152 (95%) Query: 1 MAEDIKVIVFKLGTEEYGIEVDKVQTIERMMPITRVPKTYSFIKGVINLRGVVIPVIDLR 60 M E+IKVIVF+LG EEYGIEVDKVQTIERMMPITRVPKT++F+KGVINLRGVVIPVI+LR Sbjct: 1 MGEEIKVIVFRLGEEEYGIEVDKVQTIERMMPITRVPKTFAFVKGVINLRGVVIPVINLR 60 Query: 61 GRFGIEEAEHTDQTRIIIVAVNEMEVGFIVDSANDVIDLNQDAIDTPPDVVGGIKAKYLD 120 GRFG+EE E TDQTR+IIVAVN+M+VGFIVDSA+DVIDLN D+ID+PP+VVGGIKAKYL Sbjct: 61 GRFGLEEVEATDQTRVIIVAVNDMQVGFIVDSASDVIDLNTDSIDSPPEVVGGIKAKYLR 120 Query: 121 GVAKIGEDRLLIMLNLSEVLNKNEIVQLESLE 152 GVAK+GE+RLL+MLNLSEVLNK+EI+QLE LE Sbjct: 121 GVAKVGEERLLVMLNLSEVLNKSEIIQLEGLE 152 >B1DLN4.1 B1DLN4_9BACL CheW protein Length = 153 Score = 238 bits (608), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 115/152 (75%), Positives = 138/152 (90%) Query: 1 MAEDIKVIVFKLGTEEYGIEVDKVQTIERMMPITRVPKTYSFIKGVINLRGVVIPVIDLR 60 M E++KVIVF L EEYGIEVDKV+TIER+ PITRVPKT SFIKGVINLRG+V+PVIDLR Sbjct: 1 MGEELKVIVFSLAHEEYGIEVDKVRTIERLSPITRVPKTPSFIKGVINLRGIVVPVIDLR 60 Query: 61 GRFGIEEAEHTDQTRIIIVAVNEMEVGFIVDSANDVIDLNQDAIDTPPDVVGGIKAKYLD 120 GRFG+EE+ T+ +RII+VAVNEMEVGFIVDSANDV+D++ D ID+PP++VGGIKAKYL Sbjct: 61 GRFGLEESATTENSRIIVVAVNEMEVGFIVDSANDVVDIDTDTIDSPPEIVGGIKAKYLR 120 Query: 121 GVAKIGEDRLLIMLNLSEVLNKNEIVQLESLE 152 G+AK G++RLLIMLNLSEVLNK+EI+QLE +E Sbjct: 121 GIAKFGDNRLLIMLNLSEVLNKSEIIQLEQIE 152 >C0ZF76.1 C0ZF76_BREBN Chemotaxis protein CheW Length = 158 Score = 187 bits (475), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 94/150 (62%), Positives = 126/150 (84%), Gaps = 1/150 (0%) Query: 4 DIKVIVFKLGTEEYGIEVDKVQTIERMMPITRVPKTYSFIKGVINLRGVVIPVIDLRGRF 63 ++KVIVF+L EEYG+EV +V++IE++ ITRVP+T SF+KGVINLRGVV P+IDLR RF Sbjct: 10 EVKVIVFRLKDEEYGVEVHQVKSIEKLEHITRVPRTPSFVKGVINLRGVVTPIIDLRNRF 69 Query: 64 GIEEAEHTDQTRIIIVAVNEMEVGFIVDSANDVIDLNQDAIDTPPDVVGGIKAKYLDGVA 123 +EE+ +++ TRIIIVAV E+EVG IVD+ANDVID+ DAI+ PP+VVGG++A YL GVA Sbjct: 70 SLEESVYSETTRIIIVAVGELEVGLIVDAANDVIDIPVDAIEPPPEVVGGVEAAYLRGVA 129 Query: 124 KIGEDRLLIMLNLSEVLNKNEIVQLESLEG 153 K+ + RLLI+LNL +VL+ EI QL+++EG Sbjct: 130 KL-DKRLLILLNLDKVLSTEEIKQLDAIEG 158 >B2A372.1 B2A372_NATTJ CheW protein Length = 159 Score = 171 bits (432), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 84/147 (57%), Positives = 117/147 (79%), Gaps = 1/147 (0%) Query: 4 DIKVIVFKLGTEEYGIEVDKVQTIERMMPITRVPKTYSFIKGVINLRGVVIPVIDLRGRF 63 D + IVF LG EEYG+E+ +VQTIERM+ ITRVP F++GV NLRG+V+P+IDLR R Sbjct: 10 DQQYIVFNLGDEEYGVEILQVQTIERMLDITRVPHAPDFVEGVTNLRGMVVPIIDLRKRL 69 Query: 64 GIEEAEHTDQTRIIIVAVNEMEVGFIVDSANDVIDLNQDAIDTPPDVVGGIKAKYLDGVA 123 + E E TD+TRII V ++E+ VG IVDSA+DV+ + QDAI+ PP ++GG+++ Y++GVA Sbjct: 70 NLPEKEATDETRIITVKIDEVMVGMIVDSASDVVKVPQDAIEPPPSIIGGVESTYIEGVA 129 Query: 124 KIGEDRLLIMLNLSEVLNKNEIVQLES 150 K+ E+RLLI+L LSEVL K+E+ QL++ Sbjct: 130 KL-ENRLLILLKLSEVLKKDEVDQLKN 155 >C3J423.1 C3J423_9BACI CheW protein Length = 163 Score = 169 bits (427), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 80/139 (57%), Positives = 111/139 (79%), Gaps = 1/139 (0%) Query: 4 DIKVIVFKLGTEEYGIEVDKVQTIERMMPITRVPKTYSFIKGVINLRGVVIPVIDLRGRF 63 D KVI F+L EEY + V V++IE+M PITRVP T ++KGVINLRGVV P+IDLR RF Sbjct: 26 DWKVIAFRLKEEEYALPVQHVRSIEKMQPITRVPGTAHYVKGVINLRGVVTPIIDLRERF 85 Query: 64 GIEEAEHTDQTRIIIVAVNEMEVGFIVDSANDVIDLNQDAIDTPPDVVGGIKAKYLDGVA 123 G + +QTRIIIVA+ +MEVG IVD+ANDV+DL+ ++I+ PP+ +G ++A Y+DGVA Sbjct: 86 GFAPEPYGEQTRIIIVALEDMEVGLIVDAANDVLDLSAESIEPPPEAIGSVEASYIDGVA 145 Query: 124 KIGEDRLLIMLNLSEVLNK 142 K+ E+RLLI+L+L++VL++ Sbjct: 146 KV-ENRLLILLDLAKVLDR 163 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.141 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 828,604,263 Number of extensions: 32341100 Number of successful extensions: 84045 Number of sequences better than 1.0e-05: 1924 Number of HSP's gapped: 83300 Number of HSP's successfully gapped: 1996 Length of query: 153 Length of database: 3,060,696,203 Length adjustment: 115 Effective length of query: 38 Effective length of database: 1,977,279,478 Effective search space: 75136620164 Effective search space used: 75136620164 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)