BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0034.p (165 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIE8.1 B3KIE8_9BACI CheD family protein 220 3e-56 B1DLN7.1 B1DLN7_9BACL CheD 202 6e-51 C0ZF74.1 C0ZF74_BREBN Chemotaxis protein CheD 186 5e-46 Q2B7I0.1 Q2B7I0_9BACI Protein required for methylation of methyl... 169 7e-41 B7GG94.1 B7GG94_ANOFW Chemotaxis protein; stimulates methylation... 167 4e-40 >B3KIE8.1 B3KIE8_9BACI CheD family protein Length = 165 Score = 220 bits (560), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 107/165 (64%), Positives = 124/165 (75%) Query: 1 MIEEQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIARE 60 MIEEQ+++KV MADLNV ++RTTGLGSCVG+TL+DP K+ GMAHVMLPSS IARE Sbjct: 1 MIEEQAIVKVAMADLNVAGPNGILRTTGLGSCVGLTLYDPVTKIGGMAHVMLPSSAIARE 60 Query: 61 GQMNIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESC 120 GQ+N+AK+ADTALP KMAGG+QMFAFAG DTMRIGPRNVESC Sbjct: 61 GQLNLAKYADTALPILLNKMRNLGASPARMVAKMAGGAQMFAFAGSGDTMRIGPRNVESC 120 Query: 121 KLALEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 K LE + I LIAEDTGGN+GRT+E+ C TG IRSVQKG KE+ Sbjct: 121 KAGLEQMGITLIAEDTGGNYGRTVELDCATGKFTIRSVQKGIKEL 165 >B1DLN7.1 B1DLN7_9BACL CheD Length = 164 Score = 202 bits (515), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 100/162 (61%), Positives = 120/162 (74%) Query: 4 EQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQM 63 +Q VIKVGMADL + + +RTTGLGSCVG+TL+DP ++AGMAHVMLPSSEIARE M Sbjct: 3 QQDVIKVGMADLKWAAGGAHLRTTGLGSCVGLTLYDPVARIAGMAHVMLPSSEIARESTM 62 Query: 64 NIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLA 123 NIAK+ADTA+PE KMAGG+QMFAF+G SD+MRIGPRNVESC Sbjct: 63 NIAKYADTAIPELIGQMKAEGAVIGRLAAKMAGGAQMFAFSGQSDSMRIGPRNVESCLEI 122 Query: 124 LEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 L IP++ +DTGGN+GRTIEI ++G+L IRSVQ G KEI Sbjct: 123 LANYSIPVLGQDTGGNYGRTIEIDSSSGVLTIRSVQFGIKEI 164 >C0ZF74.1 C0ZF74_BREBN Chemotaxis protein CheD Length = 161 Score = 186 bits (472), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 90/159 (56%), Positives = 112/159 (70%) Query: 7 VIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIAREGQMNIA 66 +IK+GMADL V S +RTTGLGSCVGV L+D K+AGMAHVMLP S +A+ G++ I Sbjct: 3 IIKIGMADLGVAKPPSKLRTTGLGSCVGVVLYDHIHKIAGMAHVMLPESSLAKGGELTIG 62 Query: 67 KFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESCKLALEA 126 K+ADTA+P K+AGG+QMFAF G +DTMRIGPRNVE+CKLAL+ Sbjct: 63 KYADTAIPHLIQLMVKAGAETRHTVAKLAGGAQMFAFLGNNDTMRIGPRNVEACKLALKD 122 Query: 127 LKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 I ++AEDTGGN GRTIE+ GIL IR+V +G KE+ Sbjct: 123 AHIKIVAEDTGGNCGRTIELDATNGILQIRTVNQGVKEV 161 >Q2B7I0.1 Q2B7I0_9BACI Protein required for methylation of methyl-accepting chemotaxis protein (MCPs) Length = 165 Score = 169 bits (428), Expect = 7e-41, Method: Compositional matrix adjust. Identities = 85/165 (51%), Positives = 108/165 (65%) Query: 1 MIEEQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIARE 60 M E S+IKVG+ADL + S ++RT+GLGSCVG ++D LAGMAH+MLP S +AR Sbjct: 1 MNEIASIIKVGIADLKIASMPDILRTSGLGSCVGAAVYDQSSGLAGMAHIMLPDSSLARA 60 Query: 61 GQMNIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAFAGGSDTMRIGPRNVESC 120 G +N K+ADTA+ E K+AGG+QMF F+ GSD MRIGPRNVE+ Sbjct: 61 GSINKLKYADTAIEELVRQMVRHGASPGFLRAKIAGGAQMFQFSSGSDLMRIGPRNVEAV 120 Query: 121 KLALEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 K+AL+ I LIAED GGN GRTIE T +L I++V KG E+ Sbjct: 121 KIALQVHNIRLIAEDVGGNSGRTIEFNTKTSMLQIKTVNKGVTEL 165 >B7GG94.1 B7GG94_ANOFW Chemotaxis protein; stimulates methylation of MCP proteins by CheR Length = 170 Score = 167 bits (422), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 84/166 (50%), Positives = 111/166 (66%), Gaps = 1/166 (0%) Query: 1 MIEEQSVIKVGMADLNVGSEESLVRTTGLGSCVGVTLFDPGKKLAGMAHVMLPSSEIARE 60 M E V+KVG+AD+NV ++RT GLGSCVGV ++D K++AGMAHVMLP S +A+ Sbjct: 5 MSEIVQVVKVGIADMNVVKAPHVIRTCGLGSCVGVVVYDLVKEVAGMAHVMLPDSSLAKS 64 Query: 61 GQMNIAKFADTALPEXXXXXXXXXXXXXXXXXKMAGGSQMFAF-AGGSDTMRIGPRNVES 119 MN+AK+ADTA+ E KMAGG+QMF F G +D MRIGPRNVE+ Sbjct: 65 ETMNVAKYADTAIEELIRRVIGAGGRNGHLKAKMAGGAQMFQFRTGTTDMMRIGPRNVEA 124 Query: 120 CKLALEALKIPLIAEDTGGNFGRTIEIACNTGILYIRSVQKGTKEI 165 + L+ +IP++AED GGN GRTIE TG+L IR+V +G +E+ Sbjct: 125 VREQLQRFRIPIVAEDVGGNSGRTIEFHPKTGMLLIRTVNQGVREM 170 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 810,467,370 Number of extensions: 27705155 Number of successful extensions: 53294 Number of sequences better than 1.0e-05: 230 Number of HSP's gapped: 53188 Number of HSP's successfully gapped: 230 Length of query: 165 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 40 Effective length of database: 1,883,069,328 Effective search space: 75322773120 Effective search space used: 75322773120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)