BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0020.p (72 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIG2.1 B3KIG2_9BACI Membrane-associated zinc metalloprotease 103 5e-21 B1DM60.1 B1DM60_9BACL Membrane-associated zinc metalloprotease 96 9e-19 Q9KA70.1 RASP_BACHD Zinc metalloprotease rasP EC=3.4.24.- 95 2e-18 A8UCV8.1 A8UCV8_9LACT Zinc-dependent protease, membrane associat... 94 3e-18 Q8EQU7.1 RASP_OCEIH Zinc metalloprotease rasP EC=3.4.24.- 89 2e-16 >B3KIG2.1 B3KIG2_9BACI Membrane-associated zinc metalloprotease Length = 424 Score = 103 bits (257), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 47/49 (95%), Positives = 49/49 (100%) Query: 21 HEWGHYYFAKRAGILVREFAIGFGPKLFSYKRNETQFTLRLLPFGGYAR 69 HEWGHYYFA+RAGILVREFAIGFGPKLFSYKR+ETQFTLRLLPFGGYAR Sbjct: 21 HEWGHYYFARRAGILVREFAIGFGPKLFSYKRHETQFTLRLLPFGGYAR 69 >B1DM60.1 B1DM60_9BACL Membrane-associated zinc metalloprotease Length = 421 Score = 96.3 bits (238), Expect = 9e-19, Method: Composition-based stats. Identities = 41/49 (83%), Positives = 45/49 (91%) Query: 21 HEWGHYYFAKRAGILVREFAIGFGPKLFSYKRNETQFTLRLLPFGGYAR 69 HEWGHY+FAKRAGILVREFAIGFGPKLFS KR ET+FTLRL+P GG+ R Sbjct: 21 HEWGHYFFAKRAGILVREFAIGFGPKLFSIKRGETRFTLRLVPAGGFVR 69 >Q9KA70.1 RASP_BACHD Zinc metalloprotease rasP EC=3.4.24.- Length = 420 Score = 95.1 bits (235), Expect = 2e-18, Method: Composition-based stats. Identities = 40/49 (81%), Positives = 44/49 (89%) Query: 21 HEWGHYYFAKRAGILVREFAIGFGPKLFSYKRNETQFTLRLLPFGGYAR 69 HEWGH YFAKRAGIL REFAIGFGPKLFS+KRNET +T+RL+P GGY R Sbjct: 18 HEWGHLYFAKRAGILCREFAIGFGPKLFSWKRNETVYTIRLIPLGGYVR 66 >A8UCV8.1 A8UCV8_9LACT Zinc-dependent protease, membrane associated (Putative) Length = 424 Score = 94.4 bits (233), Expect = 3e-18, Method: Composition-based stats. Identities = 38/49 (77%), Positives = 45/49 (91%) Query: 21 HEWGHYYFAKRAGILVREFAIGFGPKLFSYKRNETQFTLRLLPFGGYAR 69 HE+GHYYFAK+AGILVREFAIGFGPK+FSY++ ET FT+R+LP GGY R Sbjct: 19 HEFGHYYFAKKAGILVREFAIGFGPKIFSYRKGETTFTIRILPVGGYVR 67 >Q8EQU7.1 RASP_OCEIH Zinc metalloprotease rasP EC=3.4.24.- Length = 424 Score = 88.6 bits (218), Expect = 2e-16, Method: Composition-based stats. Identities = 36/49 (73%), Positives = 43/49 (87%) Query: 21 HEWGHYYFAKRAGILVREFAIGFGPKLFSYKRNETQFTLRLLPFGGYAR 69 HEWGH FAKRAG+LVREFAIGFGPK+FS+ +NET +T+RL+P GGY R Sbjct: 18 HEWGHLIFAKRAGMLVREFAIGFGPKIFSFTKNETLYTIRLIPAGGYVR 66 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.147 0.482 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 318,970,442 Number of extensions: 8277764 Number of successful extensions: 16925 Number of sequences better than 1.0e-05: 550 Number of HSP's gapped: 17151 Number of HSP's successfully gapped: 550 Length of query: 72 Length of database: 3,060,696,203 Length adjustment: 43 Effective length of query: 29 Effective length of database: 2,655,592,558 Effective search space: 77012184182 Effective search space used: 77012184182 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)