BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0019.p (348 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIG2.1 B3KIG2_9BACI Membrane-associated zinc metalloprotease 430 e-119 B1DM60.1 B1DM60_9BACL Membrane-associated zinc metalloprotease 272 5e-71 Q9KA70.1 RASP_BACHD Zinc metalloprotease rasP EC=3.4.24.- 187 2e-45 A6CSY2.1 A6CSY2_9BACI Putative uncharacterized protein 182 6e-44 Q2B7J4.1 Q2B7J4_9BACI Putative uncharacterized protein 174 1e-41 >B3KIG2.1 B3KIG2_9BACI Membrane-associated zinc metalloprotease Length = 424 Score = 430 bits (1106), Expect = e-119, Method: Compositional matrix adjust. Identities = 201/299 (67%), Positives = 248/299 (82%) Query: 18 QTIAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIRLDVDGEVTVYDVHPQA 77 QTIAVR+ +N+VK IYLD LD R+NVIRGE Q+ D+ + L +RLDVDGE Y++HPQA Sbjct: 84 QTIAVRVNAENQVKKIYLDQLDNRKNVIRGEVQFIDMVDRLSVRLDVDGEFQQYEIHPQA 143 Query: 78 MLIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPT 137 + + +TQIAP+DRQFGSKTVGQRA+AIFAGPVMN H+QMAGI +ENPT Sbjct: 144 LTVAKGVETQIAPRDRQFGSKTVGQRALAIFAGPVMNFILAFVLFALHIQMAGIPVENPT 203 Query: 138 YVKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEE 197 YV+IG+++ MPA+EA L++GDI+ SING IG DYQKMI L +ASK K M+WT++RGEE Sbjct: 204 YVQIGEITKGMPADEADLKEGDIIESINGTAIGADYQKMIELIAASKDKPMEWTVRRGEE 263 Query: 198 TYNVSMIPRSMEGEEGGKVGITPELPTRKAGFGETITKSGTAMVDTTKLIFIGFKQLINK 257 ++++++ PR+MEG+EGGKVGI PELPTR AG GETIT SGTAMVDTT +IF GF+QLI K Sbjct: 264 SFDLTLTPRTMEGQEGGKVGIVPELPTRSAGLGETITGSGTAMVDTTNIIFQGFRQLIQK 323 Query: 258 FNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFLGV 316 F+M+D+ GPV TF++TGQIA+QGI+ LTYWAAILSLYLGIFNLLPIPALDGSRLVFLG+ Sbjct: 324 FSMDDLGGPVRTFEVTGQIAKQGIEQLTYWAAILSLYLGIFNLLPIPALDGSRLVFLGI 382 >B1DM60.1 B1DM60_9BACL Membrane-associated zinc metalloprotease Length = 421 Score = 272 bits (695), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 149/300 (49%), Positives = 197/300 (65%), Gaps = 7/300 (2%) Query: 18 QTIAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIRLDVDGEVTVYDVHPQA 77 QTIAVR+ ++N+V IYLD LD R VIRGE DLE +L I LDV+GE + VHP A Sbjct: 84 QTIAVRV-KNNQVTRIYLDRLDERSGVIRGEVTGIDLEKDLFIALDVEGENDRFSVHPTA 142 Query: 78 MLIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPT 137 +LI ++TQIAP DRQFGSK+VG RA++IFAGP+MN ++Q+AG P Sbjct: 143 LLISRGKETQIAPIDRQFGSKSVGARAMSIFAGPLMNFVLAFVLFMVYIQLAG----TPQ 198 Query: 138 YVKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEE 197 + + +V+ MPAE A LQKGD++ ++NG KIG DY KMI + AS GK +Q + R Sbjct: 199 GLLVDEVTKGMPAEHAQLQKGDLIDTVNGVKIGTDYDKMIDIIGASAGKSIQLNVIRNGA 258 Query: 198 TYNVSMIPRSMEGEEGGKVGITPELPTRKAGFGETITKSGTAMVDTTKLIFIGFKQ-LIN 256 + + P + + + GKVG+ R A GET+T + M T IF GFK+ +I Sbjct: 259 LEPIKLTPVA-DDQGVGKVGLRAAYQFRSATVGETVTGAAKLMKTMTVSIFEGFKKIIIG 317 Query: 257 KFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFLGV 316 F ++D+ GPV T +MT +IA++GI LT W A+LSLYLGIFNLLPIPALDGSRL+FLG+ Sbjct: 318 DFKLDDLGGPVRTAEMTSEIARKGITDLTSWTALLSLYLGIFNLLPIPALDGSRLIFLGL 377 >Q9KA70.1 RASP_BACHD Zinc metalloprotease rasP EC=3.4.24.- Length = 420 Score = 187 bits (474), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 115/307 (37%), Positives = 172/307 (56%), Gaps = 20/307 (6%) Query: 20 IAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIR-LDVDGEVTVYDVHPQAM 78 + + + N V I +++ + + DLE EL I D DGE +DV P+A Sbjct: 83 VGLVFNEKNVVSRIIVNNKSKHPEAQVVQVERIDLERELFIEAYDDDGERKRWDVDPKAE 142 Query: 79 LIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPTY 138 +++ + TQIAP DRQFGSK+V QRA+AIFAGP+MN + M GI +E+P Sbjct: 143 MVQDEEATQIAPYDRQFGSKSVAQRALAIFAGPLMNFVLAFVLLAAYGFMQGIPVEDPV- 201 Query: 139 VKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEET 198 +G+++ N AE AGLQKGD V+SI+G+ + + M + +E+ + ++R + Sbjct: 202 --VGNIAENSAAETAGLQKGDYVLSIDGQTL-ETWVDMTMIIQQHPNEEITFEVERAGQI 258 Query: 199 YNVSMIPRSMEGEEGGKVGIT------PELPTRKAG--FGETITKSGTAMV-DTTKLIFI 249 + + P +EG +G +G+ PE T +G FG T T + M+ D +L+ Sbjct: 259 LQIPVTPNQVEGMDGEPIGLVGIERPAPEPATLVSGLQFGATQTYTYMTMIFDVLRLLVT 318 Query: 250 GFKQLINKFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGS 309 G +F+++ ++GPVG TGQ A+ GI L W A LS+ LGI NLLP+PALDG Sbjct: 319 G------QFSLDYVAGPVGIVNYTGQAAEMGIFVLLQWTAALSVNLGIVNLLPLPALDGG 372 Query: 310 RLVFLGV 316 RLVFLG+ Sbjct: 373 RLVFLGL 379 >A6CSY2.1 A6CSY2_9BACI Putative uncharacterized protein Length = 422 Score = 182 bits (461), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 110/302 (36%), Positives = 179/302 (59%), Gaps = 12/302 (3%) Query: 20 IAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIRLDVDGE--VTVYDVHPQA 77 + + L +D ++ I L++ D N+ E + +DLE++L IR DGE + + + A Sbjct: 87 VGLILNEDETIQKIVLNNKDKYPNIRVVEVEQSDLEHKLFIRGYEDGEDELKTFPISRDA 146 Query: 78 MLIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIENPT 137 M+I+ +TQIAP DRQF SKT+GQRA+AIFAGP+ N + G+ + P Sbjct: 147 MIIEDGIETQIAPWDRQFASKTLGQRAMAIFAGPLFNFILAFFIFLLVGILQGVPVNEPV 206 Query: 138 YVKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRGEE 197 +G ++ + A+E+GLQ+GD V+SI+G +I ++ ++++ G ++ +T+ R Sbjct: 207 ---LGKLTEDGAAKESGLQQGDQVLSIDGNEIS-TWEDIVTVIQQHPGDQLLFTIDRNGN 262 Query: 198 TYNVSMIPRS--MEGEEGGKVGITPELPTRKAGFGETITKSGTAMVDTTKLIFIGFKQLI 255 T +++ P+ +E +E G +G+ P K+ + I+ + TKLIF+ +L+ Sbjct: 263 TEELTVTPKPQVIEDKEIGIIGVHS--PVEKSPL-KVISNGFEQTYEWTKLIFVMLGKLV 319 Query: 256 N-KFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPALDGSRLVFL 314 +F+++ +SGPVG +Q T +A+ GI YL W AILS+ LGI NLLPIPALDG RL+F Sbjct: 320 TGQFSIDALSGPVGIYQSTDIVAKSGIYYLMRWGAILSINLGIMNLLPIPALDGGRLMFF 379 Query: 315 GV 316 V Sbjct: 380 AV 381 >Q2B7J4.1 Q2B7J4_9BACI Putative uncharacterized protein Length = 425 Score = 174 bits (440), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 111/311 (35%), Positives = 173/311 (55%), Gaps = 24/311 (7%) Query: 20 IAVRLGQDNKVKNIYLDSLDTRRNVIRGEAQYTDLENELKIRLDVDGE----VTVYDVHP 75 + + L D +V I L++ D + E +Y D+E++L IR +GE + Y ++ Sbjct: 83 VGLILDNDERVSKIILNNKDKYPDARPIEVEYADIEHDLVIRGYAEGEEEERLVSYPINK 142 Query: 76 QAMLIKGNQQTQIAPKDRQFGSKTVGQRAIAIFAGPVMNXXXXXXXXXXHLQMAGIKIEN 135 A+L++ +T IAP DRQF SKT+GQR +AIFAGP+MN + G+ Sbjct: 143 DALLVEDGVETLIAPYDRQFASKTLGQRTMAIFAGPMMNFILAFVVFVLIGLLQGMPTNE 202 Query: 136 PTYVKIGDVSANMPAEEAGLQKGDIVVSINGEKIGGDYQKMISLTSASKGKEMQWTLQRG 195 P ++G ++ + A+E+GL +GD + SING +I + ++ + S GKE+ + L R Sbjct: 203 P---ELGRLTPDGAAKESGLLEGDQIQSINGAEIS-SWNDVVEIIQKSPGKELDFILSRD 258 Query: 196 EETYNVSMIP--RSMEGEEG----GKVGITPEL---PTRKAGFGETITKSGTAMVDTTKL 246 E + + P R +EGE+G G +G+ + P + +G T + TK Sbjct: 259 GEELEIPVTPEAREVEGEKGKETIGIIGVYSPMEKSPLKAIAYGAEETYT------WTKE 312 Query: 247 IFIGFKQLIN-KFNMNDISGPVGTFQMTGQIAQQGIQYLTYWAAILSLYLGIFNLLPIPA 305 IF+ +L+ +F+++ +SGPVG + T +A+ GI YL WA ILS+ LGI NLLPIPA Sbjct: 313 IFVMLGKLVTGQFSIDALSGPVGIYVSTDTVAKSGIYYLMKWAGILSINLGIMNLLPIPA 372 Query: 306 LDGSRLVFLGV 316 LDG RL+F V Sbjct: 373 LDGGRLMFFAV 383 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,812,957,521 Number of extensions: 73773320 Number of successful extensions: 167361 Number of sequences better than 1.0e-05: 1226 Number of HSP's gapped: 165749 Number of HSP's successfully gapped: 1229 Length of query: 348 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 212 Effective length of database: 1,779,438,163 Effective search space: 377240890556 Effective search space used: 377240890556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)