BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0018.p (482 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIG3.1 B3KIG3_9BACI Prolyl-tRNA synthetase EC=6.1.1.15 888 0.0 B1DM59.1 B1DM59_9BACL Prolyl-tRNA synthetase 845 0.0 C0Z678.1 C0Z678_BREBN Prolyl-tRNA synthetase EC=6.1.1.15 744 0.0 Q67PA6.1 SYP_SYMTH Prolyl-tRNA synthetase EC=6.1.1.15 723 0.0 C1PQZ0.1 C1PQZ0_ALIAC Prolyl-tRNA synthetase EC=6.1.1.15 698 0.0 >B3KIG3.1 B3KIG3_9BACI Prolyl-tRNA synthetase EC=6.1.1.15 Length = 485 Score = 888 bits (2295), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/481 (85%), Positives = 448/481 (93%) Query: 2 AKDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGFEIWEHIQEEMNRR 61 +KDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDG+EIWEHIQ E++RR Sbjct: 5 SKDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGYEIWEHIQAELDRR 64 Query: 62 FKETGHRNAYFPMFIPESFFQKEKDHIEGFNPELPWVTEAGGDQLEERLAIRPTSETMIG 121 FK TGHRNAYFP+FIPESFFQKEK+H+EGFNPELPWVTEA G++LEERLAIRPTSETM G Sbjct: 65 FKATGHRNAYFPLFIPESFFQKEKEHVEGFNPELPWVTEAAGEKLEERLAIRPTSETMFG 124 Query: 122 HMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFIRTSEFLWQEGHTAHENETEAREETMQ 181 HMYSKWIQSYRDLPVLINQWANVVRWEKRTLPF+RTSEFLWQEGHTAHE E EAR ETMQ Sbjct: 125 HMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFLRTSEFLWQEGHTAHETEAEARGETMQ 184 Query: 182 MLEIYRDFVEGYLAIPVITGQKTPSERFAGAVDTYSIEAMMKDGRAVQAGTSHYLGTKFA 241 ML+IY+ FVE YLAIPVI GQKTPSE+FAGAVDT+SIEAMMKDGRAVQAGTSHY+GT F+ Sbjct: 185 MLDIYQQFVEDYLAIPVIKGQKTPSEKFAGAVDTFSIEAMMKDGRAVQAGTSHYMGTNFS 244 Query: 242 VAFEIQYLSRDNVLEYVHTTSWGTSTRLIGSMIMVHGDDRGLALPPKVAPTQVIMIPIGP 301 AF+IQYL+R+N LEY HTTSWG STRLIGS+IMVHGDDRGL LPPKVAPTQVIMIPIGP Sbjct: 245 QAFDIQYLNRENTLEYAHTTSWGVSTRLIGSLIMVHGDDRGLVLPPKVAPTQVIMIPIGP 304 Query: 302 PKTRDAVIGRTDELFKELKSAGIRVRVDDRADVTPGWKFNEYEMRGVPVRLELGPRDMEN 361 PKTRDAV+GRTDELF+ELKSAGIRVRVDD DV PGWKFNEYEMRGVPVRLE+GPRDMEN Sbjct: 305 PKTRDAVVGRTDELFRELKSAGIRVRVDDNPDVRPGWKFNEYEMRGVPVRLEIGPRDMEN 364 Query: 362 GVCVLVSRISGEKKVIQQENLVQEVQAMLEQVHNEMFERALKFREDHFYSVDTLDEMKAS 421 GVCVLVSRI+GEKKV++Q NL+QEVQ+MLEQVH EM+ERA +FREDHFYSV+TLDEMK S Sbjct: 365 GVCVLVSRITGEKKVVEQANLIQEVQSMLEQVHQEMYERAKQFREDHFYSVETLDEMKDS 424 Query: 422 MEEKRGFALAGWCGSEECESKVKEETGATSRNIPFNPAENKQVCICCGKPAAHTVVFAKA 481 ME+KRGF LAGWCGSE CE VK+ETGATSRNIPF PAENK C+ CG+ A HTV+FA+A Sbjct: 425 MEDKRGFTLAGWCGSEACEDTVKQETGATSRNIPFEPAENKTKCLVCGEDAKHTVLFARA 484 Query: 482 Y 482 Y Sbjct: 485 Y 485 >B1DM59.1 B1DM59_9BACL Prolyl-tRNA synthetase Length = 482 Score = 845 bits (2182), Expect = 0.0, Method: Compositional matrix adjust. Identities = 384/482 (79%), Positives = 441/482 (91%) Query: 1 MAKDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGFEIWEHIQEEMNR 60 M+KDKQFVTEITPQGEDFSRWYIDVIKKA+LMDYSPVRGCIVF+P+G+E+WE+IQ +++R Sbjct: 1 MSKDKQFVTEITPQGEDFSRWYIDVIKKAELMDYSPVRGCIVFRPEGYELWENIQRDLDR 60 Query: 61 RFKETGHRNAYFPMFIPESFFQKEKDHIEGFNPELPWVTEAGGDQLEERLAIRPTSETMI 120 RFKETGHRNAYFP+FIPESFFQKEK+H+EGFNPELPWVTEA G++LEERLAIRPTSETM Sbjct: 61 RFKETGHRNAYFPLFIPESFFQKEKEHVEGFNPELPWVTEAAGEKLEERLAIRPTSETMF 120 Query: 121 GHMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFIRTSEFLWQEGHTAHENETEAREETM 180 GHMY+KWIQSYRDLP+LINQWANVVRWEKRTLPF+RTSEFLWQEGHTAHE E EAREETM Sbjct: 121 GHMYAKWIQSYRDLPLLINQWANVVRWEKRTLPFLRTSEFLWQEGHTAHETEDEAREETM 180 Query: 181 QMLEIYRDFVEGYLAIPVITGQKTPSERFAGAVDTYSIEAMMKDGRAVQAGTSHYLGTKF 240 +ML +YR+FVE +LAIPVI G+KTPSERFAGAV+TYSIEAMMKDG+AVQAGTSHYLGTKF Sbjct: 181 RMLTVYREFVEEFLAIPVIEGEKTPSERFAGAVNTYSIEAMMKDGKAVQAGTSHYLGTKF 240 Query: 241 AVAFEIQYLSRDNVLEYVHTTSWGTSTRLIGSMIMVHGDDRGLALPPKVAPTQVIMIPIG 300 AVAF+I+YL R+N L++ HTTSWG STRLIG+MIMVHGDDRGLALPPKVAPTQVIMIPIG Sbjct: 241 AVAFDIKYLDRENTLQFAHTTSWGVSTRLIGAMIMVHGDDRGLALPPKVAPTQVIMIPIG 300 Query: 301 PPKTRDAVIGRTDELFKELKSAGIRVRVDDRADVTPGWKFNEYEMRGVPVRLELGPRDME 360 P KTRD V+GR DEL+ ELK AG+RV+VDDR+DV+PGWKFNEYEMRGVP+RLELGPRDME Sbjct: 301 PAKTRDQVVGRVDELYAELKKAGVRVKVDDRSDVSPGWKFNEYEMRGVPLRLELGPRDME 360 Query: 361 NGVCVLVSRISGEKKVIQQENLVQEVQAMLEQVHNEMFERALKFREDHFYSVDTLDEMKA 420 NG VL SR+SGEK++++Q+NL+ EV+ ML + +M+E+A +FRED+FY V+TLDEMKA Sbjct: 361 NGQVVLASRVSGEKRIVRQDNLLAEVETMLADIQRDMYEKAKQFREDNFYDVETLDEMKA 420 Query: 421 SMEEKRGFALAGWCGSEECESKVKEETGATSRNIPFNPAENKQVCICCGKPAAHTVVFAK 480 SMEEKRGF LAGWCGS CE +VKEETGATSRNIPF P + K C+ CG A HTVVFA+ Sbjct: 421 SMEEKRGFVLAGWCGSAACEKQVKEETGATSRNIPFEPEKKKSKCLVCGDHAEHTVVFAR 480 Query: 481 AY 482 AY Sbjct: 481 AY 482 >C0Z678.1 C0Z678_BREBN Prolyl-tRNA synthetase EC=6.1.1.15 Length = 488 Score = 744 bits (1920), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/489 (71%), Positives = 404/489 (82%), Gaps = 8/489 (1%) Query: 1 MAKDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGFEIWEHIQEEMNR 60 M +DK FV EITPQ EDFSRWYID IKKADLMDY+PVRGCIVFKPDGFE+WE IQE MN+ Sbjct: 1 MKEDKAFVKEITPQSEDFSRWYIDAIKKADLMDYTPVRGCIVFKPDGFELWERIQEAMNK 60 Query: 61 RFKETGHRNAYFPMFIPESFFQKEKDHIEGFNPELPWVTEAGGDQLEERLAIRPTSETMI 120 RFKETGHRNAYFPM IPESFFQKEK+HIEGFNPELPWVTEAGG+ LEE+LA+RPTSET+I Sbjct: 61 RFKETGHRNAYFPMLIPESFFQKEKEHIEGFNPELPWVTEAGGEPLEEKLALRPTSETII 120 Query: 121 GHMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFIRTSEFLWQEGHTAHENETEAREETM 180 GHMYS+WIQSYRDLPVLINQWANV RWEKRT+PF+RTSEFLWQEGHTAH E EAR+ETM Sbjct: 121 GHMYSQWIQSYRDLPVLINQWANVFRWEKRTMPFLRTSEFLWQEGHTAHATEEEARQETM 180 Query: 181 QMLEIYRDFVEGYLAIPVITGQKTPSERFAGAVDTYSIEAMMKDGRAVQAGTSHYLGTKF 240 QMLEIYR+ VE LAIPV GQKTPSERFAGAVDTYSIEAMMKDG+AVQAGTSHYLG KF Sbjct: 181 QMLEIYREVVEQELAIPVWKGQKTPSERFAGAVDTYSIEAMMKDGKAVQAGTSHYLGDKF 240 Query: 241 AVAFEIQYLSRDNVLEYVHTTSWGTSTRLIGSMIMVHGDDRGLALPPKVAPTQVIMIPIG 300 A FEI++L RDN +YVHTTSWGTSTRLIGSMIMVHGDDRGL LPP++APTQVIMIP+G Sbjct: 241 ARGFEIKFLDRDNQFKYVHTTSWGTSTRLIGSMIMVHGDDRGLVLPPRMAPTQVIMIPVG 300 Query: 301 PPKTRDAVIGRTDELFKELKSAGIRVRVDDRADVTPGWKFNEYEMRGVPVRLELGPRDME 360 P K R+ V+ D LF E+K+AG+RVR D R + TPGWKFNE+EMRGVP+RLELGPRD+E Sbjct: 301 PMKLREKVMEAFDPLFDEIKAAGVRVRADLREE-TPGWKFNEWEMRGVPLRLELGPRDVE 359 Query: 361 NGVCVLVSRISGEKKVIQQENLVQEVQAMLEQVHNEMFERALKFR-EDHFYSVDTLDEMK 419 NG +L R +GEK + E + Q V +LE++ MF++AL FR E+ +DTL+++ Sbjct: 360 NGQVILARRDTGEKVTVSLEGIAQTVVQLLEEIQQNMFQKALAFRDENSHLGIDTLEQLS 419 Query: 420 A------SMEEKRGFALAGWCGSEECESKVKEETGATSRNIPFNPAENKQVCICCGKPAA 473 A S + G+ LAGWCG + CESK+KEET TSRNIPF P K+ CICCGK + Sbjct: 420 AHIAKNESENKTSGWVLAGWCGDDACESKIKEETKFTSRNIPFEPPVQKKACICCGKESQ 479 Query: 474 HTVVFAKAY 482 H+V F +AY Sbjct: 480 HSVWFGRAY 488 >Q67PA6.1 SYP_SYMTH Prolyl-tRNA synthetase EC=6.1.1.15 Length = 488 Score = 723 bits (1867), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/489 (70%), Positives = 411/489 (84%), Gaps = 8/489 (1%) Query: 1 MAKDKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGFEIWEHIQEEMNR 60 M ++K FV EITPQ EDFSRWYIDVI+KADLMDY+PVRGCIVFKPDG+E+WE IQ +++ Sbjct: 1 MKEEKSFVKEITPQSEDFSRWYIDVIRKADLMDYTPVRGCIVFKPDGYELWERIQAGLDK 60 Query: 61 RFKETGHRNAYFPMFIPESFFQKEKDHIEGFNPELPWVTEAGGDQLEERLAIRPTSETMI 120 RFKETGHRNAYFPM IPESFFQKEK+H+EGFNPELPWVTEAGG++LEERLA+RPTSET+I Sbjct: 61 RFKETGHRNAYFPMLIPESFFQKEKEHVEGFNPELPWVTEAGGEKLEERLALRPTSETII 120 Query: 121 GHMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFIRTSEFLWQEGHTAHENETEAREETM 180 GHMY++WIQSYRDLPVLINQWANV RWEKRTLPF+RTSEFLWQEGHTAH E EAREETM Sbjct: 121 GHMYAQWIQSYRDLPVLINQWANVFRWEKRTLPFLRTSEFLWQEGHTAHATEEEAREETM 180 Query: 181 QMLEIYRDFVEGYLAIPVITGQKTPSERFAGAVDTYSIEAMMKDGRAVQAGTSHYLGTKF 240 +MLE+YRDFVE +AIP+ GQKTPSE+FAGAVDTYSIEAMMKDG+A+QAGTSHYLG F Sbjct: 181 RMLEVYRDFVETEMAIPLYVGQKTPSEKFAGAVDTYSIEAMMKDGKALQAGTSHYLGQNF 240 Query: 241 AVAFEIQYLSRDNVLEYVHTTSWGTSTRLIGSMIMVHGDDRGLALPPKVAPTQVIMIPIG 300 A FEI++L RDN L+YVHTTSWG STRLIG++IMVHGDDRGLALPP++AP QVIMIP+G Sbjct: 241 AKGFEIKFLDRDNQLKYVHTTSWGVSTRLIGALIMVHGDDRGLALPPRLAPIQVIMIPVG 300 Query: 301 PPKTRDAVIGRTDELFKELKSAGIRVRVDDRADVTPGWKFNEYEMRGVPVRLELGPRDME 360 PPK RD V+ R D LF LK+AG+RV+ D R + TPGWKFNE+EMRGVP+R+E+GPRD++ Sbjct: 301 PPKFRDKVMARFDPLFDALKAAGVRVKADLREE-TPGWKFNEWEMRGVPLRIEIGPRDVD 359 Query: 361 NGVCVLVSRISGEKKVIQQENLVQEVQAMLEQVHNEMFERALKFREDHFY-SVDTLDEMK 419 N C++V R +GEK + + V+ VQA+LE++ MF +A +FR+ H + ++TL E+K Sbjct: 360 NKQCLMVRRDTGEKIPVPLDEAVERVQALLEEIQRNMFVKAKEFRDAHSHLHINTLAELK 419 Query: 420 ASME------EKRGFALAGWCGSEECESKVKEETGATSRNIPFNPAENKQVCICCGKPAA 473 A + E GF LAGWCG CE+KVKEET TSRNIPF+P KQVC+CCGK A Sbjct: 420 AHIARCEETGEIAGFVLAGWCGDAACEAKVKEETKFTSRNIPFHPPARKQVCLCCGKEAQ 479 Query: 474 HTVVFAKAY 482 HTV FA+AY Sbjct: 480 HTVWFARAY 488 >C1PQZ0.1 C1PQZ0_ALIAC Prolyl-tRNA synthetase EC=6.1.1.15 Length = 483 Score = 698 bits (1801), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/483 (67%), Positives = 396/483 (81%), Gaps = 1/483 (0%) Query: 1 MAK-DKQFVTEITPQGEDFSRWYIDVIKKADLMDYSPVRGCIVFKPDGFEIWEHIQEEMN 59 MAK DKQFV EITPQ EDFSRWYIDV++KA+ MDY+PVRGCIV +PD +EIWE Q E++ Sbjct: 1 MAKQDKQFVKEITPQSEDFSRWYIDVLQKAEWMDYAPVRGCIVIRPDAYEIWERCQRELD 60 Query: 60 RRFKETGHRNAYFPMFIPESFFQKEKDHIEGFNPELPWVTEAGGDQLEERLAIRPTSETM 119 R FK TGHRNAYFP+FIPESFF+KEK+H+EGFNPELPWVTEAGGD LEERLA+RPTSETM Sbjct: 61 REFKRTGHRNAYFPLFIPESFFEKEKEHVEGFNPELPWVTEAGGDILEERLAVRPTSETM 120 Query: 120 IGHMYSKWIQSYRDLPVLINQWANVVRWEKRTLPFIRTSEFLWQEGHTAHENETEAREET 179 GHMY++WIQSYRDLP+L+NQWANVVRWEKRTLPF+RTSEFLWQEGHTAH E EAREET Sbjct: 121 FGHMYAQWIQSYRDLPLLLNQWANVVRWEKRTLPFLRTSEFLWQEGHTAHATEEEAREET 180 Query: 180 MQMLEIYRDFVEGYLAIPVITGQKTPSERFAGAVDTYSIEAMMKDGRAVQAGTSHYLGTK 239 M+ML IY DFVE +LAIPVI GQKTPSE+FAGAV+TY+IE++MKDGRA+Q TSHYLG Sbjct: 181 MRMLGIYADFVESFLAIPVIRGQKTPSEKFAGAVETYTIESLMKDGRALQTATSHYLGQN 240 Query: 240 FAVAFEIQYLSRDNVLEYVHTTSWGTSTRLIGSMIMVHGDDRGLALPPKVAPTQVIMIPI 299 FA +FEIQYL +DN L+YV+TTSWG STR++G+++MVHGDDRGL LPPK++PTQV+++PI Sbjct: 241 FAKSFEIQYLDKDNQLKYVYTTSWGMSTRILGAIVMVHGDDRGLVLPPKLSPTQVVIVPI 300 Query: 300 GPPKTRDAVIGRTDELFKELKSAGIRVRVDDRADVTPGWKFNEYEMRGVPVRLELGPRDM 359 GP K R+AV+ + ELF LK A IRV +DDR DV+PGWKFNEYE+RGVP+RLELGPRD+ Sbjct: 301 GPAKAREAVVAKARELFDALKRADIRVHLDDRDDVSPGWKFNEYELRGVPIRLELGPRDL 360 Query: 360 ENGVCVLVSRISGEKKVIQQENLVQEVQAMLEQVHNEMFERALKFREDHFYSVDTLDEMK 419 E VL R +GEK + + +++ VQA+L + ++FE A + E++ + +TLD++ Sbjct: 361 EASQAVLARRDTGEKITVPLDAVIERVQALLSDIQRDLFEAAKRRLEENSHVAETLDQLV 420 Query: 420 ASMEEKRGFALAGWCGSEECESKVKEETGATSRNIPFNPAENKQVCICCGKPAAHTVVFA 479 ++ KRGF LAGWCG + CE VK+ ATSR IPF+P C+ CGKPA H V FA Sbjct: 421 EIIQTKRGFVLAGWCGDDACERAVKDACTATSRCIPFDPPARPSTCVTCGKPAKHAVWFA 480 Query: 480 KAY 482 K+Y Sbjct: 481 KSY 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,987,879,986 Number of extensions: 127845779 Number of successful extensions: 318412 Number of sequences better than 1.0e-05: 1870 Number of HSP's gapped: 316034 Number of HSP's successfully gapped: 1873 Length of query: 482 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 342 Effective length of database: 1,741,754,103 Effective search space: 595679903226 Effective search space used: 595679903226 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)