BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0015.p (403 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIG4.1 B3KIG4_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 726 0.0 B1DM57.1 B1DM57_9BACL DNA-directed DNA polymerase EC=2.7.7.7 705 0.0 A4IMD2.1 A4IMD2_GEOTN DNA polymerase III alpha subunit 558 e-157 B4BJ04.1 B4BJ04_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 557 e-157 C5D9C4.1 C5D9C4_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 554 e-156 >B3KIG4.1 B3KIG4_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1438 Score = 726 bits (1874), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/403 (85%), Positives = 365/403 (90%) Query: 1 VKRSTGQHPGGIVVLPDYMEIEDITPVQYPADDVTAEWKTTHFDYHAFDANLLKLDILGH 60 VKRSTGQHPGGIVV+PDY+E+E+ITPVQYPADDV AEWKTTHFDYHAF+ANLLKLDILGH Sbjct: 1036 VKRSTGQHPGGIVVVPDYIEVEEITPVQYPADDVNAEWKTTHFDYHAFEANLLKLDILGH 1095 Query: 61 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVSPEQIRTPVATYGVPEMGTKF 120 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGV PEQIR+PVAT+GVPEMGTKF Sbjct: 1096 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVKPEQIRSPVATFGVPEMGTKF 1155 Query: 121 VRQMLVESKPSSFADLLQISGLSHGTGVWLGNAQELIKNNTCNIKTVIGCRDDIMLYLIY 180 VRQMLVES+PSSFADLLQISGLSHGTGVWLGNAQELIKN TCNIKTVIGCRDDIML+LIY Sbjct: 1156 VRQMLVESQPSSFADLLQISGLSHGTGVWLGNAQELIKNGTCNIKTVIGCRDDIMLFLIY 1215 Query: 181 KAGMDAGLAFKITESVRKGKGLSAEWIEEMKKCKVPGWYIDSCLKIQYMFPKAHAAAYVI 240 KAGMDA LAFKITESVRKG+GLS EWI+EMKKCKVP WYIDSCLKIQYMFPKAHAAAYVI Sbjct: 1216 KAGMDASLAFKITESVRKGRGLSQEWIDEMKKCKVPQWYIDSCLKIQYMFPKAHAAAYVI 1275 Query: 241 SAVRTAYFKLYHPIEYYATYFSVRAADFDIELCCKGYEAIGRQIVEIEQKGFQALPKEKA 300 SAVRTAYFKLY+PIEYYATYFSVRA DFDIELCC+GYEAI R+I EIEQ GF A PKEK Sbjct: 1276 SAVRTAYFKLYYPIEYYATYFSVRAEDFDIELCCQGYEAIYRRIEEIEQLGFGASPKEKG 1335 Query: 301 MLPVLEMALEMTARGFTFKNIDLYRSDATKFTVDGTSLIPPFSSLXXXXXXXXXXXXXXX 360 MLP+LEMALEMTARGF+FKNIDLY+SDA +F VDG SLIPPFS+L Sbjct: 1336 MLPILEMALEMTARGFSFKNIDLYQSDALRFKVDGDSLIPPFSALAGIGDNAARNIAAAR 1395 Query: 361 XXXXFLSIEDFQQKSKASKTVVELLNGMGCFRGLPESNQLSLF 403 FLSIEDFQQKSKASKT VELL MGCFRGLPESNQLSLF Sbjct: 1396 EFGEFLSIEDFQQKSKASKTAVELLTQMGCFRGLPESNQLSLF 1438 >B1DM57.1 B1DM57_9BACL DNA-directed DNA polymerase EC=2.7.7.7 Length = 495 Score = 705 bits (1819), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/403 (82%), Positives = 362/403 (89%) Query: 1 VKRSTGQHPGGIVVLPDYMEIEDITPVQYPADDVTAEWKTTHFDYHAFDANLLKLDILGH 60 VKRSTGQHPGGIVV+P+YME+EDITPVQ+PADDV AEWKTTHFDYHAF+ANLLKLDILGH Sbjct: 93 VKRSTGQHPGGIVVVPNYMEVEDITPVQFPADDVNAEWKTTHFDYHAFEANLLKLDILGH 152 Query: 61 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVSPEQIRTPVATYGVPEMGTKF 120 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNST+AL VSPE+IRTPVATYGVPEMGTKF Sbjct: 153 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTKALEVSPEKIRTPVATYGVPEMGTKF 212 Query: 121 VRQMLVESKPSSFADLLQISGLSHGTGVWLGNAQELIKNNTCNIKTVIGCRDDIMLYLIY 180 VRQML E++PSSFADLLQISGLSHGTGVWLGNAQELIK TCNIKTVIGCRDDIMLYLIY Sbjct: 213 VRQMLQETQPSSFADLLQISGLSHGTGVWLGNAQELIKKGTCNIKTVIGCRDDIMLYLIY 272 Query: 181 KAGMDAGLAFKITESVRKGKGLSAEWIEEMKKCKVPGWYIDSCLKIQYMFPKAHAAAYVI 240 KAGMDAGLAFKITESVRKGKGL+ EWIEEMK+CKVP WYIDSCL+I+YMFPKAHAAAYVI Sbjct: 273 KAGMDAGLAFKITESVRKGKGLTPEWIEEMKRCKVPAWYIDSCLRIEYMFPKAHAAAYVI 332 Query: 241 SAVRTAYFKLYHPIEYYATYFSVRAADFDIELCCKGYEAIGRQIVEIEQKGFQALPKEKA 300 SAVRTAYFKLY+PI YYATYFSVRA DFD+EL C+GY+AI ++I+EIE KGF A KEK Sbjct: 333 SAVRTAYFKLYYPIHYYATYFSVRAEDFDLELLCQGYDAIQKKIIEIEDKGFNATTKEKN 392 Query: 301 MLPVLEMALEMTARGFTFKNIDLYRSDATKFTVDGTSLIPPFSSLXXXXXXXXXXXXXXX 360 + +LEMALEMTARGF+FK IDLYRSDATKF VDG +LIPPFS++ Sbjct: 393 SISLLEMALEMTARGFSFKPIDLYRSDATKFIVDGETLIPPFSAIGGIGENAARNIAAAK 452 Query: 361 XXXXFLSIEDFQQKSKASKTVVELLNGMGCFRGLPESNQLSLF 403 FLSIEDFQQ+SKASKT++E+L GMGCFRGLPESNQLSLF Sbjct: 453 DAGEFLSIEDFQQRSKASKTIIEVLTGMGCFRGLPESNQLSLF 495 >A4IMD2.1 A4IMD2_GEOTN DNA polymerase III alpha subunit Length = 1442 Score = 558 bits (1437), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/403 (64%), Positives = 316/403 (78%), Gaps = 1/403 (0%) Query: 1 VKRSTGQHPGGIVVLPDYMEIEDITPVQYPADDVTAEWKTTHFDYHAFDANLLKLDILGH 60 VKR+TGQHPGGI+V+PDYMEI D TP+QYPADD ++EW+TTHFD+H+ NLLKLDILGH Sbjct: 1041 VKRTTGQHPGGIIVVPDYMEIYDFTPIQYPADDTSSEWRTTHFDFHSIHDNLLKLDILGH 1100 Query: 61 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVSPEQIRTPVATYGVPEMGTKF 120 DDPT++RMLQDL+G+DP TIP +DP VM +F+STE LGV+PEQI V T G+PE GT+F Sbjct: 1101 DDPTVIRMLQDLSGIDPKTIPTDDPDVMGIFSSTEPLGVTPEQIMCNVGTIGIPEFGTRF 1160 Query: 121 VRQMLVESKPSSFADLLQISGLSHGTGVWLGNAQELIKNNTCNIKTVIGCRDDIMLYLIY 180 VRQML E+KP +F++L+QISGLSHGT VWLGNAQELI+N TC + VIGCRDDIM+YLIY Sbjct: 1161 VRQMLEETKPKTFSELVQISGLSHGTDVWLGNAQELIQNGTCTLSEVIGCRDDIMVYLIY 1220 Query: 181 KAGMDAGLAFKITESVRKGKGLSAEWIEEMKKCKVPGWYIDSCLKIQYMFPKAHAAAYVI 240 + G++ LAFKI ESVRKGKGL+ E+ EEM+K VP WYIDSC KI+YMFPKAHAAAYV+ Sbjct: 1221 R-GLEPSLAFKIMESVRKGKGLTPEFEEEMRKHDVPEWYIDSCKKIKYMFPKAHAAAYVL 1279 Query: 241 SAVRTAYFKLYHPIEYYATYFSVRAADFDIELCCKGYEAIGRQIVEIEQKGFQALPKEKA 300 AVR AYFK++HP+ YYA+YF+VRA DFD++ KG AI ++I EI KG QA KEK+ Sbjct: 1280 MAVRIAYFKVHHPLLYYASYFTVRAEDFDLDAMIKGSAAIRKRIEEINAKGIQATAKEKS 1339 Query: 301 MLPVLEMALEMTARGFTFKNIDLYRSDATKFTVDGTSLIPPFSSLXXXXXXXXXXXXXXX 360 +L VLE+ALEM RGFTFKNIDLYRS AT+F +DG SLIPPF+++ Sbjct: 1340 LLTVLEVALEMCERGFTFKNIDLYRSQATEFVIDGHSLIPPFNAIPGLGTNVAQNIVRAR 1399 Query: 361 XXXXFLSIEDFQQKSKASKTVVELLNGMGCFRGLPESNQLSLF 403 FLS ED QQ+ K SKT++E L GC LP+ NQLSLF Sbjct: 1400 EEGEFLSKEDLQQRGKVSKTLLEYLESRGCLDSLPDHNQLSLF 1442 >B4BJ04.1 B4BJ04_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1434 Score = 557 bits (1436), Expect = e-157, Method: Compositional matrix adjust. Identities = 260/403 (64%), Positives = 316/403 (78%), Gaps = 1/403 (0%) Query: 1 VKRSTGQHPGGIVVLPDYMEIEDITPVQYPADDVTAEWKTTHFDYHAFDANLLKLDILGH 60 VKR+TGQHPGGI+V+PDYMEI D TP+QYPADD ++EW+TTHFD+H+ NLLKLDILGH Sbjct: 1033 VKRTTGQHPGGIIVVPDYMEIYDFTPIQYPADDTSSEWRTTHFDFHSIHDNLLKLDILGH 1092 Query: 61 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVSPEQIRTPVATYGVPEMGTKF 120 DDPT++RMLQDL+G+DP TIP +DP VM +F+STE LGV+PEQI V T G+PE GT+F Sbjct: 1093 DDPTVIRMLQDLSGIDPKTIPTDDPDVMGIFSSTEPLGVTPEQIMCNVGTIGIPEFGTRF 1152 Query: 121 VRQMLVESKPSSFADLLQISGLSHGTGVWLGNAQELIKNNTCNIKTVIGCRDDIMLYLIY 180 VRQML E+KP +F++L+QISGLSHGT VWLGNAQELI+N TC + VIGCRDDIM+YLIY Sbjct: 1153 VRQMLEETKPKTFSELVQISGLSHGTDVWLGNAQELIQNGTCTLSEVIGCRDDIMVYLIY 1212 Query: 181 KAGMDAGLAFKITESVRKGKGLSAEWIEEMKKCKVPGWYIDSCLKIQYMFPKAHAAAYVI 240 + G++ LAFKI ESVRKGKGL+ E+ EEM+K VP WYIDSC KI+YMFPKAHAAAYV+ Sbjct: 1213 R-GLEPSLAFKIMESVRKGKGLTPEFEEEMRKHDVPEWYIDSCKKIKYMFPKAHAAAYVL 1271 Query: 241 SAVRTAYFKLYHPIEYYATYFSVRAADFDIELCCKGYEAIGRQIVEIEQKGFQALPKEKA 300 AVR AYFK++HP+ YYA+YF+VRA DFD++ KG AI ++I EI KG QA KEK+ Sbjct: 1272 MAVRIAYFKVHHPLLYYASYFTVRAEDFDLDAMIKGSAAIRKRIEEINAKGIQATAKEKS 1331 Query: 301 MLPVLEMALEMTARGFTFKNIDLYRSDATKFTVDGTSLIPPFSSLXXXXXXXXXXXXXXX 360 +L VLE+ALEM RGFTFKNIDLYRS AT+F +DG SLIPPF+++ Sbjct: 1332 LLTVLEVALEMCERGFTFKNIDLYRSQATEFVIDGHSLIPPFNAIPGLGTNVAQNIVRAR 1391 Query: 361 XXXXFLSIEDFQQKSKASKTVVELLNGMGCFRGLPESNQLSLF 403 FLS ED QQ+ K SKT++E L GC LP+ NQLSLF Sbjct: 1392 EEGEFLSKEDLQQRGKVSKTLLEYLESRGCLDSLPDHNQLSLF 1434 >C5D9C4.1 C5D9C4_9BACI DNA polymerase III, alpha subunit EC=2.7.7.7 Length = 1435 Score = 554 bits (1428), Expect = e-156, Method: Compositional matrix adjust. Identities = 256/403 (63%), Positives = 317/403 (78%), Gaps = 1/403 (0%) Query: 1 VKRSTGQHPGGIVVLPDYMEIEDITPVQYPADDVTAEWKTTHFDYHAFDANLLKLDILGH 60 VKR+TGQHPGGI+V+PDYM+I D TP+QYPADD ++EW+TTHFD+H+ NLLKLDILGH Sbjct: 1034 VKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDTSSEWRTTHFDFHSIHDNLLKLDILGH 1093 Query: 61 DDPTMMRMLQDLTGVDPTTIPMNDPKVMSMFNSTEALGVSPEQIRTPVATYGVPEMGTKF 120 DDPT++RMLQDL+G+DP TIP +DP+VM +F+STE+LGV+PEQI V T G+PE GT+F Sbjct: 1094 DDPTVIRMLQDLSGIDPKTIPTDDPEVMKIFSSTESLGVTPEQIMCNVGTLGIPEFGTRF 1153 Query: 121 VRQMLVESKPSSFADLLQISGLSHGTGVWLGNAQELIKNNTCNIKTVIGCRDDIMLYLIY 180 VRQML E+KP +F++L+QISGLSHGT VWLGNAQELI+N TC + VIGCRDDIM+YLIY Sbjct: 1154 VRQMLEETKPKTFSELVQISGLSHGTDVWLGNAQELIQNGTCTLSEVIGCRDDIMVYLIY 1213 Query: 181 KAGMDAGLAFKITESVRKGKGLSAEWIEEMKKCKVPGWYIDSCLKIQYMFPKAHAAAYVI 240 + G++ LAFKI ESVRKGKGL+ E+ EEM+K VP WYI+SC KI+YMFPKAHAAAYV+ Sbjct: 1214 R-GLEPSLAFKIMESVRKGKGLTPEFEEEMRKHDVPEWYIESCKKIKYMFPKAHAAAYVL 1272 Query: 241 SAVRTAYFKLYHPIEYYATYFSVRAADFDIELCCKGYEAIGRQIVEIEQKGFQALPKEKA 300 AVR AYFK++HP+ YYA+YF+VRA DFD++ KG AI ++I EI KG A KEK+ Sbjct: 1273 MAVRIAYFKVHHPLLYYASYFTVRAEDFDLDAMIKGSAAIRKRIEEINAKGLNATAKEKS 1332 Query: 301 MLPVLEMALEMTARGFTFKNIDLYRSDATKFTVDGTSLIPPFSSLXXXXXXXXXXXXXXX 360 +L VLE+ALEM RGF+FKNIDLYRS AT+F +DG SLIPPF+++ Sbjct: 1333 LLTVLEVALEMCERGFSFKNIDLYRSQATEFVIDGNSLIPPFNAIPGLGTNVALNIVRAR 1392 Query: 361 XXXXFLSIEDFQQKSKASKTVVELLNGMGCFRGLPESNQLSLF 403 FLS ED QQ+ K SKT++E L GC LP+ NQLSLF Sbjct: 1393 EEGEFLSKEDLQQRGKLSKTLIEYLESRGCLDSLPDHNQLSLF 1435 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,324,444,611 Number of extensions: 91818506 Number of successful extensions: 193728 Number of sequences better than 1.0e-05: 461 Number of HSP's gapped: 192514 Number of HSP's successfully gapped: 461 Length of query: 403 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 265 Effective length of database: 1,760,596,133 Effective search space: 466557975245 Effective search space used: 466557975245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)