BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0586.0003.p (328 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIH6.1 B3KIH6_9BACI Polysaccharide deacetylase 400 e-109 Q9KA84.1 Q9KA84_BACHD BH2406 protein 295 5e-78 C1PG87.1 C1PG87_BACCO Sporulation protein, polysaccharide deacet... 281 5e-74 Q5WFU9.1 Q5WFU9_BACSK Polysaccharide deacetylase 281 7e-74 C5D9D6.1 C5D9D6_9BACI Sporulation protein, polysaccharide deacet... 280 1e-73 >B3KIH6.1 B3KIH6_9BACI Polysaccharide deacetylase Length = 330 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 191/275 (69%), Positives = 223/275 (81%), Gaps = 2/275 (0%) Query: 52 DLRASIESKAAKLNSPPVDATVDRVWKAIPGYNGLEVDVEATYREA--KLKGPNDGLKLL 109 DL IE+ AAK PVDA VD VWKAIPGYNGLEVD+EATY+ A K + D + + Sbjct: 53 DLLGQIEAAAAKSRIEPVDARVDSVWKAIPGYNGLEVDIEATYQAASSKKRKAGDAIPYV 112 Query: 110 YRQIKPKVSLDDLGAQPIYRGNPAKPMVSLMINVAWGNQYIIPMLDILDQEKVKVTFFLD 169 YRQI KV+LDDLG QPIYRGNP KPMVS+MINVAWGN++++PML+ LD EKVK TFFLD Sbjct: 113 YRQIPAKVTLDDLGEQPIYRGNPNKPMVSMMINVAWGNEFLVPMLNTLDAEKVKTTFFLD 172 Query: 170 GSWLSRNKELAAEMLKRGHEMENHAYTHPNMSTLSRARATAEIQKTQNLLKESLGVTNIW 229 GSWL +N ELAAE++KRGHE+ENHA+THPNMS LSR RA EI KTQ+LLKESLGV N W Sbjct: 173 GSWLKKNPELAAEIVKRGHEVENHAFTHPNMSRLSRERAEMEIAKTQDLLKESLGVVNKW 232 Query: 230 FAPPSGDFDQETVDIAASLGLKTVLWTLDTVDWRNPSPDXXXXXXXXQAEPGTLILMHPT 289 FAPPSGDF+Q+TV IA + GLKTVLWTLDTVDW++P+PD + EPG LILMHPT Sbjct: 233 FAPPSGDFNQQTVSIAHARGLKTVLWTLDTVDWKHPAPDAVVQKITSKVEPGFLILMHPT 292 Query: 290 ASSSQALRGMIRGIKAKGLQLGTVSQTLSAERVLP 324 SSSQAL+GMI GI+AKGL++GTVSQTLS ER++P Sbjct: 293 DSSSQALKGMIDGIRAKGLEIGTVSQTLSPERIMP 327 >Q9KA84.1 Q9KA84_BACHD BH2406 protein Length = 323 Score = 295 bits (754), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 139/306 (45%), Positives = 201/306 (65%), Gaps = 11/306 (3%) Query: 22 TYGPVK-----EMLAQLKPQTGLAVWGRVYTEAQDDLRASIESKAAKLNSPPVDATVDRV 76 TYG V+ + + QLK + L V + D L I+ +A + P +DA +DRV Sbjct: 18 TYGAVQNPFSSQYIGQLK-EEALPV-----AKMTDSLYEEIKDRAPEYEQPAIDAKIDRV 71 Query: 77 WKAIPGYNGLEVDVEATYREAKLKGPNDGLKLLYRQIKPKVSLDDLGAQPIYRGNPAKPM 136 WKAIPGYNGLEVDVE++Y K +G D L++R+ KP V LDDL P++RGNP KPM Sbjct: 72 WKAIPGYNGLEVDVESSYNRMKQEGRFDERYLVFRETKPSVHLDDLPPSPVFRGNPEKPM 131 Query: 137 VSLMINVAWGNQYIIPMLDILDQEKVKVTFFLDGSWLSRNKELAAEMLKRGHEMENHAYT 196 V+L++NVAWGN+++ ML +++ VK TFFLDGSW+ ++ +LA +++ GHE+ NHAY+ Sbjct: 132 VTLLVNVAWGNEHLPTMLKTMNKYDVKSTFFLDGSWVKKHPQLATMIVEEGHEIGNHAYS 191 Query: 197 HPNMSTLSRARATAEIQKTQNLLKESLGVTNIWFAPPSGDFDQETVDIAASLGLKTVLWT 256 HP+M L+R R EI +T ++K ++ VT WFAPPSG ++ V AA G++T++W+ Sbjct: 192 HPDMQRLTRERMDEEIVQTNEVIKATIEVTPKWFAPPSGSYNDLVVQRAAEHGMRTIMWS 251 Query: 257 LDTVDWRNPSPDXXXXXXXXQAEPGTLILMHPTASSSQALRGMIRGIKAKGLQLGTVSQT 316 +DT+DWRNP P+ + PG +ILMHPT SS+ L +IRGI+ +GL +GTVS Sbjct: 252 VDTIDWRNPDPNEMVDRVLSKVHPGAMILMHPTESSAAGLENLIRGIQDRGLHIGTVSDL 311 Query: 317 LSAERV 322 + R+ Sbjct: 312 MDESRI 317 >C1PG87.1 C1PG87_BACCO Sporulation protein, polysaccharide deacetylase family Length = 368 Score = 281 bits (719), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 130/278 (46%), Positives = 187/278 (67%) Query: 46 YTEAQDDLRASIESKAAKLNSPPVDATVDRVWKAIPGYNGLEVDVEATYREAKLKGPNDG 105 ++ +++L+ IE A + PP +A +DRVWKAIPGYNG+EVD +A+Y + K G D Sbjct: 69 FSTKENELQEKIEQAAERFYRPPENAKIDRVWKAIPGYNGVEVDKKASYSKMKQDGRYDE 128 Query: 106 LKLLYRQIKPKVSLDDLGAQPIYRGNPAKPMVSLMINVAWGNQYIIPMLDILDQEKVKVT 165 KL+++QI P V L DL P+Y GNP KPMV+ +INVAWGN+Y+ ML L + + T Sbjct: 129 RKLVFKQIPPAVHLKDLDPAPVYTGNPDKPMVAFLINVAWGNEYLPDMLKTLKKHHLHAT 188 Query: 166 FFLDGSWLSRNKELAAEMLKRGHEMENHAYTHPNMSTLSRARATAEIQKTQNLLKESLGV 225 FFL+G W N ELA ++ GHE NH+YTHP+ STL ++ +++ KT +L+ Sbjct: 189 FFLEGRWAKENPELARMIVSGGHETGNHSYTHPDFSTLPESKIKSQLVKTNRVLEAITEE 248 Query: 226 TNIWFAPPSGDFDQETVDIAASLGLKTVLWTLDTVDWRNPSPDXXXXXXXXQAEPGTLIL 285 WFAPPSG + E V IA S+G++T++WT+DTVDW+NPSP+ +A+ G+LIL Sbjct: 249 KVKWFAPPSGSYRDEAVSIAKSMGMETIMWTVDTVDWQNPSPETIVERVTAKAQGGSLIL 308 Query: 286 MHPTASSSQALRGMIRGIKAKGLQLGTVSQTLSAERVL 323 MHPTAS+++AL +I ++ K LQ+GTVS+ L ER++ Sbjct: 309 MHPTASTAKALEPLIARLEKKNLQVGTVSKLLDEERII 346 >Q5WFU9.1 Q5WFU9_BACSK Polysaccharide deacetylase Length = 333 Score = 281 bits (719), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 126/273 (46%), Positives = 189/273 (69%) Query: 50 QDDLRASIESKAAKLNSPPVDATVDRVWKAIPGYNGLEVDVEATYREAKLKGPNDGLKLL 109 +++L I++ A K+ P +A +D+VWKA+PGYNGLEVD+E +Y + K G + KL+ Sbjct: 46 ENELLKQIKAHAEKVAIPATNARIDKVWKALPGYNGLEVDIEQSYNKMKESGSFNQSKLV 105 Query: 110 YRQIKPKVSLDDLGAQPIYRGNPAKPMVSLMINVAWGNQYIIPMLDILDQEKVKVTFFLD 169 +++ KP V L+DL P Y+G+ KPMVS +INVAWGN+Y+ ML +L Q VK TFFLD Sbjct: 106 FKETKPDVHLEDLPPAPTYKGHEEKPMVSFLINVAWGNEYLPDMLKVLQQYDVKATFFLD 165 Query: 170 GSWLSRNKELAAEMLKRGHEMENHAYTHPNMSTLSRARATAEIQKTQNLLKESLGVTNIW 229 GSW+ +N +LA + + GHE+ +HAY+HP+M+ LS+A E+ KT +++ +L VT W Sbjct: 166 GSWVKKNPQLAVMIKEEGHEIGSHAYSHPHMNALSKAEIDEELTKTTEVIEATLDVTPKW 225 Query: 230 FAPPSGDFDQETVDIAASLGLKTVLWTLDTVDWRNPSPDXXXXXXXXQAEPGTLILMHPT 289 FAPPSG+F+Q V+ AA+ ++T+LWT+DT+DW+ P P Q + G+++LMHPT Sbjct: 226 FAPPSGEFNQLVVERAAAYDMRTILWTVDTIDWKKPEPSVMVERVVNQMQAGSMLLMHPT 285 Query: 290 ASSSQALRGMIRGIKAKGLQLGTVSQTLSAERV 322 SSS+ L +I+G++ K LQ+GTVS + R+ Sbjct: 286 ESSSKGLEAIIQGMQEKQLQIGTVSDLMDETRI 318 >C5D9D6.1 C5D9D6_9BACI Sporulation protein, polysaccharide deacetylase family Length = 321 Score = 280 bits (716), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 126/273 (46%), Positives = 181/273 (66%) Query: 51 DDLRASIESKAAKLNSPPVDATVDRVWKAIPGYNGLEVDVEATYREAKLKGPNDGLKLLY 110 D L A I KA + PP DA +DRVWKA PGYNGL++DV+A+Y K +G D KL+Y Sbjct: 47 DKLYAEIVEKAKQYEIPPQDAVIDRVWKATPGYNGLKIDVQASYENMKKEGKFDERKLVY 106 Query: 111 RQIKPKVSLDDLGAQPIYRGNPAKPMVSLMINVAWGNQYIIPMLDILDQEKVKVTFFLDG 170 +Q+ P+V L DL PIYRG+P KPMV+ +INVAWGN+YI MLDIL + VK TFFL+G Sbjct: 107 KQVPPRVHLGDLPPAPIYRGHPDKPMVAFLINVAWGNEYIPKMLDILKKHNVKATFFLEG 166 Query: 171 SWLSRNKELAAEMLKRGHEMENHAYTHPNMSTLSRARATAEIQKTQNLLKESLGVTNIWF 230 W+ + ++A ++ GHE+ NH+Y+HP+ +L + E+ KT +++ + GV WF Sbjct: 167 RWVKNHPDMAKMIVDAGHEIGNHSYSHPDFKSLDNEKIRKELVKTNEIIEATTGVKCKWF 226 Query: 231 APPSGDFDQETVDIAASLGLKTVLWTLDTVDWRNPSPDXXXXXXXXQAEPGTLILMHPTA 290 APPSG + + V IAA L +KT++W++DT+DW+ PSP + PG +ILMHPT Sbjct: 227 APPSGSYRDDVVKIAADLDMKTIMWSVDTIDWQKPSPSVIVRRVMSKVHPGAMILMHPTL 286 Query: 291 SSSQALRGMIRGIKAKGLQLGTVSQTLSAERVL 323 S+++AL +I I+ K ++G VS L +R++ Sbjct: 287 STAEALEPLIISIQQKEYEIGNVSTLLDEKRIV 319 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,748,406,142 Number of extensions: 66844051 Number of successful extensions: 126577 Number of sequences better than 1.0e-05: 1979 Number of HSP's gapped: 125278 Number of HSP's successfully gapped: 1983 Length of query: 328 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 192 Effective length of database: 1,779,438,163 Effective search space: 341652127296 Effective search space used: 341652127296 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)