BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0067.p (361 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C2Q4C9.1 C2Q4C9_BACCE Salicylate esterase 318 5e-85 C3BJI5.1 C3BJI5_9BACI Salicylate esterase 283 2e-74 C3AKR1.1 C3AKR1_BACMY Salicylate esterase 283 2e-74 C3B2N3.1 C3B2N3_BACMY Salicylate esterase 281 1e-73 Q9RBI4.1 Q9RBI4_ACIAD Salicylate esterase 243 2e-62 >C2Q4C9.1 C2Q4C9_BACCE Salicylate esterase Length = 245 Score = 318 bits (815), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 150/241 (62%), Positives = 182/241 (75%), Gaps = 1/241 (0%) Query: 120 LTFVLVHGAWVDASFWDGIAAELRKMGHTVYTPEYAGHGADPDKNVTHEQETQSVVDFIK 179 LTFV++HG+W D S +DG+AAEL K+GH+VY PEY GHG D NVTH+Q T +V+++I+ Sbjct: 5 LTFVIIHGSWADVSQFDGVAAELHKLGHSVYVPEYPGHGVLADNNVTHQQITNAVIEYIE 64 Query: 180 QKDLHHIILLAHSFGGTVIQKTAEQVPDRLKRLVFFDAFAPLDGQSAADQFPPPAAELFL 239 ++ L HI+LL HSFGGT++ A+Q+P R+ RLVF +AF PLDGQS DQ PPP E F Sbjct: 65 KRGLKHIVLLGHSFGGTIVATVAQQIPHRIDRLVFSNAFVPLDGQSLYDQLPPPLQETFK 124 Query: 240 GLSKASGNNTIMLPFDLFRDTFVNTATLEQAKLLYKKVIPEPAGPLFQKLDLKKFYSLKT 299 L+ ASGNNTIMLPF LFRD F NTATLE+A +Y K+IPEPAGPLFQKLDLKKFY+L+ Sbjct: 125 QLADASGNNTIMLPFPLFRDAFTNTATLEEATSMYHKIIPEPAGPLFQKLDLKKFYNLQI 184 Query: 300 PKSYLYLTEDIAMPELPSYGWHPVQSGHLGVYRFIEGTGDHMAAPHYAPKEIADKIVQAS 359 PKSYL LTEDIA+P S+ WH QS HLG YR+IEG GDH PK IA K V+A Sbjct: 185 PKSYLNLTEDIALPP-GSFAWHTNQSSHLGFYRYIEGNGDHFTTFFREPKMIAKKFVEAG 243 Query: 360 R 360 R Sbjct: 244 R 244 >C3BJI5.1 C3BJI5_9BACI Salicylate esterase Length = 298 Score = 283 bits (723), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 135/277 (48%), Positives = 183/277 (66%), Gaps = 10/277 (3%) Query: 90 AFLVLLTALPF-----GGAAAEASALKSKPAPSGALTFVLVHGAWVDASFWDGIAAELRK 144 A L++L++ F + ++ KP +TFVLVHGAW D+S+WD A EL++ Sbjct: 27 AILIMLSSNSFHIPAEAKIETDIDVIRQKP-----ITFVLVHGAWGDSSYWDKTANELKQ 81 Query: 145 MGHTVYTPEYAGHGADPDKNVTHEQETQSVVDFIKQKDLHHIILLAHSFGGTVIQKTAEQ 204 MGH VYTP GHG D +K V H +SVV+++K++++ +L+ HSFGGTVI K AEQ Sbjct: 82 MGHKVYTPTLPGHGKDTNKAVKHTDYVKSVVNYVKERNITDFVLVGHSFGGTVISKVAEQ 141 Query: 205 VPDRLKRLVFFDAFAPLDGQSAADQFPPPAAELFLGLSKASGNNTIMLPFDLFRDTFVNT 264 +PDR+ RLVF +AF +G+SAAD+ P L+ L+K S NN I LPF ++R+TF+N Sbjct: 142 IPDRIHRLVFMNAFVLANGESAADEIPAEGKTLWTELAKKSKNNAIQLPFPIWRETFMNN 201 Query: 265 ATLEQAKLLYKKVIPEPAGPLFQKLDLKKFYSLKTPKSYLYLTEDIAMPELPSYGWHPVQ 324 A L+ AK +Y+ V PEPAGPLF+KLDL KFY L PKSY YLTED+A+P+ GWHP Sbjct: 202 ANLDLAKKIYETVTPEPAGPLFEKLDLTKFYQLNIPKSYFYLTEDMAVPQGKDSGWHPHM 261 Query: 325 SGHLGVYRFIEGTGDHMAAPHYAPKEIADKIVQASRD 361 S LG++R + GDHM H P +A K+V+A RD Sbjct: 262 SNRLGLFRLVTTQGDHMTMFHAKPAIVAKKLVEAGRD 298 >C3AKR1.1 C3AKR1_BACMY Salicylate esterase Length = 298 Score = 283 bits (723), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 135/277 (48%), Positives = 183/277 (66%), Gaps = 10/277 (3%) Query: 90 AFLVLLTALPF-----GGAAAEASALKSKPAPSGALTFVLVHGAWVDASFWDGIAAELRK 144 A L++L++ F + ++ KP +TFVLVHGAW D+S+WD A EL++ Sbjct: 27 AILIMLSSNSFHIPAEAKIETDIDVIRQKP-----ITFVLVHGAWGDSSYWDKTANELKQ 81 Query: 145 MGHTVYTPEYAGHGADPDKNVTHEQETQSVVDFIKQKDLHHIILLAHSFGGTVIQKTAEQ 204 MGH VYTP GHG D +K V H +SVV+++K++++ +L+ HSFGGTVI K AEQ Sbjct: 82 MGHKVYTPTLPGHGKDTNKAVKHTDYVKSVVNYVKERNITDFVLVGHSFGGTVISKVAEQ 141 Query: 205 VPDRLKRLVFFDAFAPLDGQSAADQFPPPAAELFLGLSKASGNNTIMLPFDLFRDTFVNT 264 +PDR+ RLVF +AF +G+SAAD+ P L+ L+K S NN I LPF ++R+TF+N Sbjct: 142 IPDRIHRLVFMNAFVLANGESAADEIPAEGKTLWTELAKKSKNNAIQLPFPIWRETFMNN 201 Query: 265 ATLEQAKLLYKKVIPEPAGPLFQKLDLKKFYSLKTPKSYLYLTEDIAMPELPSYGWHPVQ 324 A L+ AK +Y+ V PEPAGPLF+KLDL KFY L PKSY YLTED+A+P+ GWHP Sbjct: 202 ANLDLAKKIYETVTPEPAGPLFEKLDLTKFYQLNIPKSYFYLTEDMAVPQGKDSGWHPHM 261 Query: 325 SGHLGVYRFIEGTGDHMAAPHYAPKEIADKIVQASRD 361 S LG++R + GDHM H P +A K+V+A RD Sbjct: 262 SNRLGLFRLVTTQGDHMTMFHAKPAIVAKKLVEAGRD 298 >C3B2N3.1 C3B2N3_BACMY Salicylate esterase Length = 268 Score = 281 bits (718), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 131/256 (51%), Positives = 175/256 (68%), Gaps = 5/256 (1%) Query: 106 EASALKSKPAPSGALTFVLVHGAWVDASFWDGIAAELRKMGHTVYTPEYAGHGADPDKNV 165 + ++ KP +TFVLVHGAW D+S+WD A EL++MGH VYTP GHG D +K V Sbjct: 18 DIDVIRQKP-----ITFVLVHGAWGDSSYWDKTANELKQMGHKVYTPTLPGHGKDTNKAV 72 Query: 166 THEQETQSVVDFIKQKDLHHIILLAHSFGGTVIQKTAEQVPDRLKRLVFFDAFAPLDGQS 225 H +SVV+++K++++ +L+ HSFGGTVI K AEQ+PDR+ RLVF +AF +G+S Sbjct: 73 KHTDYVKSVVNYVKERNITDFVLVGHSFGGTVISKVAEQIPDRIHRLVFMNAFVLANGES 132 Query: 226 AADQFPPPAAELFLGLSKASGNNTIMLPFDLFRDTFVNTATLEQAKLLYKKVIPEPAGPL 285 AAD+ P L+ L+K S NN I LPF ++R+TF+N A L+ AK +Y+ V PEPAGPL Sbjct: 133 AADEIPAEGKTLWTELAKKSKNNAIQLPFPIWRETFMNNANLDLAKKIYETVTPEPAGPL 192 Query: 286 FQKLDLKKFYSLKTPKSYLYLTEDIAMPELPSYGWHPVQSGHLGVYRFIEGTGDHMAAPH 345 F+KLDL KFY L PKSY YLTED+A+P+ GWHP S LG++R + GDHM H Sbjct: 193 FEKLDLTKFYQLNIPKSYFYLTEDMAVPQGKDSGWHPHMSNRLGLFRLVTTQGDHMTMFH 252 Query: 346 YAPKEIADKIVQASRD 361 P +A K+V+A RD Sbjct: 253 AKPAIVAKKLVEAGRD 268 >Q9RBI4.1 Q9RBI4_ACIAD Salicylate esterase Length = 239 Score = 243 bits (621), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 115/242 (47%), Positives = 162/242 (66%), Gaps = 4/242 (1%) Query: 120 LTFVLVHGAWVDASFWDGIAAELRKMGHTVYTPEYAGHGADPDKNVTHEQETQSVVDFIK 179 +T+VLVHG+W D S W+ +A LR GHTV+ P AGHG + D+NVTH Q +QS+ D+I Sbjct: 2 ITYVLVHGSWHDGSLWEPVATHLRAQGHTVHCPTVAGHGPNADRNVTHAQCSQSIADYIV 61 Query: 180 QKDLHHIILLAHSFGGTVIQKTAEQVPDRLKRLVFFDAFAPLDGQSAADQFPPPAAELFL 239 + DL I+LL HS+GGT+I K AE +P+R++RL++++AF DG++ D P ELF Sbjct: 62 KHDLSEIVLLGHSYGGTIISKVAEAIPERIQRLIYWNAFVLQDGENMFDNMPEAYYELFT 121 Query: 240 GLSKASGNNTIMLPFDLFRDTFVNTATLEQAKLLYKKVIPEPAGPLFQKLDLKKFYSLKT 299 L+ ASG+NT++LP++++R F+N A + A+ YK + PEP P +LDLKKFY+L Sbjct: 122 SLAAASGDNTVLLPYEVWRHAFINDADDQMAEETYKMLTPEPCQPFHDRLDLKKFYTLNI 181 Query: 300 PKSYLYLTEDIAMPELPSYGWHPVQSGHLGVYRFIEGTGDHMAAPHYAPKEIADKIVQAS 359 PKSYL TED A LP+ WHP S LG ++ +E G H A P+E+A KI++AS Sbjct: 182 PKSYLNCTEDQA---LPAGFWHPKMSNRLGEFKLVEMGGSHEAM-FTRPQELATKIIEAS 237 Query: 360 RD 361 D Sbjct: 238 HD 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,278,758,995 Number of extensions: 97881621 Number of successful extensions: 227578 Number of sequences better than 1.0e-05: 277 Number of HSP's gapped: 228179 Number of HSP's successfully gapped: 277 Length of query: 361 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 224 Effective length of database: 1,770,017,148 Effective search space: 396483841152 Effective search space used: 396483841152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)