BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0064.p (245 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0FH70.1 C0FH70_9CLOT tRNA (guanine-N(1)-)-methyltransferase EC=... 128 4e-28 B1D4U5.1 B1D4U5_9BACL HAD-superfamily hydrolase, subfamily IA, v... 78 8e-13 B3KH77.1 B3KH77_9BACI HAD-superfamily hydrolase, subfamily IA, v... 76 3e-12 C5CJS4.1 C5CJS4_VARPD HAD-superfamily hydrolase, subfamily IA, v... 74 2e-11 A1VRJ3.1 A1VRJ3_POLNA HAD-superfamily hydrolase, subfamily IA, v... 72 5e-11 >C0FH70.1 C0FH70_9CLOT tRNA (guanine-N(1)-)-methyltransferase EC=2.1.1.31 Length = 490 Score = 128 bits (322), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 81/248 (32%), Positives = 129/248 (52%), Gaps = 17/248 (6%) Query: 9 ERISVNPYQVISLDMFQTLVNIEGRRNHIWRPILQQDFSEARALELGQALLTHYFAQAAA 68 E+ + Y+V+S D+FQTL ++ R IW+ IL + ++E RA +A+++ Y Sbjct: 245 EKAARASYRVLSFDLFQTLADVNARIPEIWKEILGEHYTERRARTGAEAVVSAY---GKL 301 Query: 69 IRDA---GSFCTSQEIYRRGFEKV-----FQEHLLSFDCHEAVDILFAEHRLSGLYADTV 120 +RD+ F +E+Y R E+V F + +++ H + +Y D V Sbjct: 302 LRDSFRKRKFLDMEEMYLRCSEEVLRVTGFGGEKFGVSREQMAEVILKNHSQAPVYEDVV 361 Query: 121 PFLQKISEAYQICIVSDTDTLM----LPDFYKEYPI--KLFTSEDYGSYKNDGRNRMFTE 174 L+K+ E Y++ + SD LM L KE ++F S++ K F Sbjct: 362 SVLKKLGERYRLILSSDASPLMAEGVLERLKKEGVTFEQVFLSDELHICKGRPDGAFFDT 421 Query: 175 VIAYYGVQPEEILHIGDSASDVLGAARAGIRSCWINRTGSSWKHTVQPDYTVSNLEELYP 234 V+ GV+PEEILHIGDS S+V GAA AG+ +C ++R + +K VQPDY + +LEE++ Sbjct: 422 VVRTLGVRPEEILHIGDSPSEVSGAAAAGVHTCLLSRDYTPYKGKVQPDYRIESLEEVFR 481 Query: 235 ILEPGAPA 242 L G A Sbjct: 482 YLNGGGSA 489 >B1D4U5.1 B1D4U5_9BACL HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 233 Score = 77.8 bits (190), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 56/161 (34%), Positives = 82/161 (50%), Gaps = 28/161 (17%) Query: 74 SFCTSQEIYRRGFEKVFQEHLLSFDCHEAVDILFAEHRLSGLYADTVPFLQKISEAYQIC 133 S C+++++ RR FE + L Y DT+PFL S I Sbjct: 82 SDCSAEQLIRRQFEHWMKPEL---------------------YEDTLPFLSTFSH-LPIY 119 Query: 134 IVSDTDTLMLPDFYKEYPIK---LFTSEDYGSYKNDGRNRMFTEVIAYYGVQPEEILHIG 190 I+S+ DT + + IK + TSED +YK R +F E I G++P EI+HIG Sbjct: 120 IISNIDTADIAAAAMFHGIKVTDIITSEDVRAYKP--RPELFLEGIKRCGLKPSEIVHIG 177 Query: 191 DS-ASDVLGAARAGIRSCWINRTGSSWKHTVQPDYTVSNLE 230 DS +SDV+GA R GI++ W+NR ++PDYT +L+ Sbjct: 178 DSYSSDVIGAGRLGIQTIWLNRLNKRQPQGIEPDYTCHDLK 218 >B3KH77.1 B3KH77_9BACI HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 223 Score = 75.9 bits (185), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 50/165 (30%), Positives = 81/165 (49%), Gaps = 4/165 (2%) Query: 74 SFCTSQEIYRRGFEKVFQEHLLSFDCHEAVDILFAEHRLSGLYADTVPFLQKIS-EAYQI 132 +F T + + R K + + + E + F + G+Y D++ FL+ Y I Sbjct: 61 NFDTQRNLGRMSLCKTIEFYQSNCTADEIILEQFEHWQKPGIYEDSLSFLKSCELPVYII 120 Query: 133 CIVSDTDTLMLPDFYKEYPIKLFTSEDYGSYKNDGRNRMFTEVIAYYGVQPEEILHIGDS 192 + +D + F+ + TSED SYK R+ +F E + Y ++ ++LH+GDS Sbjct: 121 SNIDTSDVMAAIKFHGLKVSGVITSEDVRSYK--PRSEIFAEALRRYELKNTDVLHVGDS 178 Query: 193 -ASDVLGAARAGIRSCWINRTGSSWKHTVQPDYTVSNLEELYPIL 236 SDV+GA GIR+ WINR +QP+Y + L EL I+ Sbjct: 179 LTSDVVGAQNMGIRAVWINRMNKMKPDHIQPNYIIKKLTELEGIM 223 >C5CJS4.1 C5CJS4_VARPD HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 243 Score = 73.6 bits (179), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 43/132 (32%), Positives = 66/132 (50%), Gaps = 3/132 (2%) Query: 102 AVDILFAEHRLSGLYADTVPFLQKISEAYQICIVSDTDTLMLPDFYKEYPIKLFTSEDYG 161 A D FAE + LY D +P L+ +SE Y + VS+ + + + F + +G Sbjct: 108 AFDAFFAERQRVTLYEDALPALKWLSERYPLVAVSNGNADIHQTGVGRWFRTAFNARAFG 167 Query: 162 SYKNDGRNRMFTEVIAYYGVQPEEILHIGDSAS-DVLGAARAGIRSCWINRTGSSWKHTV 220 S K +F A G+ P+++LH+GD A DV+GA AG+++ W+ R W H Sbjct: 168 SGKPHAP--IFRAAAASVGLLPKDVLHVGDDAELDVVGALNAGMQAAWLVRDERPWVHGA 225 Query: 221 QPDYTVSNLEEL 232 +P V NL L Sbjct: 226 RPQLIVPNLHAL 237 >A1VRJ3.1 A1VRJ3_POLNA HAD-superfamily hydrolase, subfamily IA, variant 1 Length = 231 Score = 71.6 bits (174), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 43/140 (30%), Positives = 69/140 (49%), Gaps = 11/140 (7%) Query: 102 AVDILFAEHRLSGLYADTVPFLQKISEAYQICIVS----DTDTLMLPDFYKEYPIKLFTS 157 A DI FA L+ D L+ ++ + + +S D + + + ++K ++ Sbjct: 96 AFDIFFAARNQVTLFGDVPQSLEFLAGRFPLVALSNGNADIERIGIGPYFKAS----LSA 151 Query: 158 EDYGSYKNDGRNRMFTEVIAYYGVQPEEILHIGDSAS-DVLGAARAGIRSCWINRTGSSW 216 + +G K D R+F GVQP E+LH+GD A+ DVL A G+++ W+NR G W Sbjct: 152 QQFGVGKPD--PRIFRAAATAAGVQPHEVLHVGDDAALDVLAALDCGMQAVWVNRAGLPW 209 Query: 217 KHTVQPDYTVSNLEELYPIL 236 H P V+ L EL +L Sbjct: 210 PHDALPHQAVATLAELCGVL 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,477,314,537 Number of extensions: 59524058 Number of successful extensions: 148293 Number of sequences better than 1.0e-05: 88 Number of HSP's gapped: 148671 Number of HSP's successfully gapped: 88 Length of query: 245 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 113 Effective length of database: 1,817,122,223 Effective search space: 205334811199 Effective search space used: 205334811199 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)