BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0061.p (247 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DD04.1 B1DD04_9BACL RelA/SpoT domain protein 350 6e-95 B3KCB7.1 B3KCB7_9BACI RelA/SpoT domain protein 348 2e-94 A8MF75.1 A8MF75_ALKOO RelA/SpoT domain protein 296 1e-78 C1PD23.1 C1PD23_BACCO RelA/SpoT domain protein 295 2e-78 A6TQ72.1 A6TQ72_ALKMQ RelA/SpoT domain protein 290 9e-77 >B1DD04.1 B1DD04_9BACL RelA/SpoT domain protein Length = 249 Score = 350 bits (898), Expect = 6e-95, Method: Compositional matrix adjust. Identities = 166/245 (67%), Positives = 204/245 (83%) Query: 3 MSTQDQIEKFKQLKYELTRFMMIYKFALEEMETKIEILKQEFQMLHDYSPIEHTKSRIKS 62 M+ + +F+QLK +TRFMM YKFAL+EMETKIEILK+EFQMLHDY+PIEHTKSR+KS Sbjct: 1 MNLLSSVSQFQQLKQLITRFMMTYKFALDEMETKIEILKEEFQMLHDYNPIEHTKSRLKS 60 Query: 63 PESIMNKMLRKNNELSLPAIRATIKDIAGLRITCSFISDIYQVSAMLQKQDDLKVLGVKD 122 PESIMNK++RKN ++SLP+I+ I+DIAGLRITCSFI DIY +S ML++Q DLKVL VKD Sbjct: 61 PESIMNKLVRKNLDISLPSIKNNIRDIAGLRITCSFIKDIYLISEMLKRQSDLKVLEVKD 120 Query: 123 YIKHPKPNGYQSLHLLVEVPVFLSDCQEHVCVEVQIRTIAMDFWASLEHKIFYKYSQSVP 182 YIK+PKPNGY+SLH+L+EVPVFLSD EHVC+EVQIRTIAMDFWASLEHKIFYKY+ +VP Sbjct: 121 YIKNPKPNGYKSLHMLIEVPVFLSDRVEHVCIEVQIRTIAMDFWASLEHKIFYKYNAAVP 180 Query: 183 EHLTRELKNAADKAYELDLQMERLHREIKEIKDAQGDDTDVELRRIMINNQQFNLPANFI 242 + L +ELK AAD LD QMERLH E+ EIK D + +LR + I+NQQFN+P + Sbjct: 181 DGLLQELKAAADSVTALDRQMERLHMEMGEIKGDNVIDAEEQLRMLQIDNQQFNIPIALL 240 Query: 243 KLLGE 247 +++GE Sbjct: 241 EMIGE 245 >B3KCB7.1 B3KCB7_9BACI RelA/SpoT domain protein Length = 247 Score = 348 bits (894), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 169/241 (70%), Positives = 202/241 (83%), Gaps = 1/241 (0%) Query: 8 QIEKFKQLKYELTRFMMIYKFALEEMETKIEILKQEFQMLHDYSPIEHTKSRIKSPESIM 67 QIE+ K ++ E+TRFMM YKFAL+E+ETK+EILK+EFQ LHDYSPIEHTKSR+KSPESIM Sbjct: 5 QIEQLKIIRNEITRFMMKYKFALDEIETKMEILKEEFQALHDYSPIEHTKSRLKSPESIM 64 Query: 68 NKMLRKNNELSLPAIRATIKDIAGLRITCSFISDIYQVSAMLQKQDDLKVLGVKDYIKHP 127 K+LRK +SL +I IKDIAGLRITCSFI+DIY++S MLQ Q DL VL VKDYIK+P Sbjct: 65 KKLLRKGGNISLRSIEDNIKDIAGLRITCSFITDIYKISDMLQNQSDLTVLNVKDYIKNP 124 Query: 128 KPNGYQSLHLLVEVPVFLSDCQEHVCVEVQIRTIAMDFWASLEHKIFYKYSQSVPEHLTR 187 KPNGYQSLHLLVEVPVF SD E VCVEVQIRTIAMDFWASLEHKIFYKY++SVP+HL Sbjct: 125 KPNGYQSLHLLVEVPVFFSDRVEKVCVEVQIRTIAMDFWASLEHKIFYKYNKSVPQHLLE 184 Query: 188 ELKNAADKAYELDLQMERLHREIKEIKDAQGDDTDV-ELRRIMINNQQFNLPANFIKLLG 246 ELK AAD A LD QME+LHRE++ IKD++ DD+ + EL+ I INN+++ LPA +++L Sbjct: 185 ELKKAADTANALDRQMEQLHREVQAIKDSESDDSLLEELKSIQINNRKYELPAGLLEVLT 244 Query: 247 E 247 E Sbjct: 245 E 245 >A8MF75.1 A8MF75_ALKOO RelA/SpoT domain protein Length = 253 Score = 296 bits (758), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 139/233 (59%), Positives = 186/233 (79%), Gaps = 2/233 (0%) Query: 10 EKFKQLKYELTRFMMIYKFALEEMETKIEILKQEFQMLHDYSPIEHTKSRIKSPESIMNK 69 ++ K LK +LTRF+M+YKF L+EM TKI IL+QEF+ +HDY+PIE+ KSR+KSPESI K Sbjct: 10 QELKNLKVDLTRFLMVYKFGLDEMNTKIHILRQEFEHIHDYNPIENVKSRLKSPESIFKK 69 Query: 70 MLRKNNELSLPAIRATIKDIAGLRITCSFISDIYQVSAMLQKQDDLKVLGVKDYIKHPKP 129 + RK S+P+I+ IKDIAG+RITCSFISDIY++S MLQKQ D+KV+ KDYIK+PKP Sbjct: 70 VYRKRCGFSIPSIKENIKDIAGIRITCSFISDIYKISEMLQKQGDVKVIDCKDYIKNPKP 129 Query: 130 NGYQSLHLLVEVPVFLSDCQEHVCVEVQIRTIAMDFWASLEHKIFYKYSQSVPEHLTREL 189 NGY+SLHL++E+PVF+SD EH VEVQIRTIAMDFWASLEHKI+YKY++ VP++L EL Sbjct: 130 NGYKSLHLILEIPVFMSDRVEHTYVEVQIRTIAMDFWASLEHKIYYKYNKEVPKNLLAEL 189 Query: 190 KNAADKAYELDLQMERLHREIKEIKDAQGDDTDVELRRIMINNQQFNLPANFI 242 K AAD +LD +ME +H E+ ++K+A D+ L ++I+N++F +P F+ Sbjct: 190 KEAADTVSQLDSKMESIHIEMNKVKEA--DNLSENLHELIISNEKFRIPYEFL 240 >C1PD23.1 C1PD23_BACCO RelA/SpoT domain protein Length = 252 Score = 295 bits (756), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 136/234 (58%), Positives = 190/234 (81%), Gaps = 3/234 (1%) Query: 10 EKFKQLKYELTRFMMIYKFALEEMETKIEILKQEFQMLHDYSPIEHTKSRIKSPESIMNK 69 E+ K++K ELTRFMM YKFAL E+ TKI+ILK+EFQ +HDY+PIEH KSR+KSPESI+NK Sbjct: 10 EQMKEVKTELTRFMMSYKFALHEVMTKIDILKEEFQYVHDYNPIEHVKSRLKSPESILNK 69 Query: 70 MLRKNNELSLPAIRATIKDIAGLRITCSFISDIYQVSAMLQKQDDLKVLGVKDYIKHPKP 129 RK LSL +IR I+DIAG+RITCSFISDIY++S M+Q+Q D+K++ KDYI++PKP Sbjct: 70 AYRKGCALSLDSIRENIRDIAGIRITCSFISDIYEISKMIQQQKDVKLIECKDYIQNPKP 129 Query: 130 NGYQSLHLLVEVPVFLSDCQEHVCVEVQIRTIAMDFWASLEHKIFYKYSQSVPEHLTREL 189 NGYQSLH+++++PVF+SD E V VE+QIRTIAMDFWASLEHKI+YKY + VP+HL EL Sbjct: 130 NGYQSLHMIIKIPVFMSDRSEEVYVEIQIRTIAMDFWASLEHKIYYKYDEDVPQHLKEEL 189 Query: 190 KNAADKAYELDLQMERLHREIKEIKDAQGDDTDVELRRIMINNQQFNLPANFIK 243 K AA +A +LD++ME + +E+KE+K +++ + +++++ ++ +P +F+K Sbjct: 190 KEAALQAAQLDVKMENIQKEMKEVKKRNSENS---VHQLIVDTEKLRIPEDFLK 240 >A6TQ72.1 A6TQ72_ALKMQ RelA/SpoT domain protein Length = 246 Score = 290 bits (741), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 137/233 (58%), Positives = 187/233 (80%), Gaps = 2/233 (0%) Query: 11 KFKQLKYELTRFMMIYKFALEEMETKIEILKQEFQMLHDYSPIEHTKSRIKSPESIMNKM 70 + K +K ELTRFMM YKFA++E+ TKI+ILKQEF+ +H+YSPIEH +SR+K+PESI+ K Sbjct: 12 QIKLIKTELTRFMMSYKFAMDELNTKIDILKQEFRYIHEYSPIEHVESRLKTPESIVKKA 71 Query: 71 LRKNNELSLPAIRATIKDIAGLRITCSFISDIYQVSAMLQKQDDLKVLGVKDYIKHPKPN 130 +K EL+LP+I+ ++DIAGLRITCSFISDIY++S ML+ Q D+KV+ KDYIK+PKPN Sbjct: 72 YKKGYELTLPSIKENVQDIAGLRITCSFISDIYELSEMLRNQKDIKVIKCKDYIKNPKPN 131 Query: 131 GYQSLHLLVEVPVFLSDCQEHVCVEVQIRTIAMDFWASLEHKIFYKYSQSVPEHLTRELK 190 GY+SLHL++++P+F+SD E V VEVQIRTIAMDFWASLEHKI+YKY + +P+ L ELK Sbjct: 132 GYKSLHLILQIPIFMSDRVEEVYVEVQIRTIAMDFWASLEHKIYYKYDKEIPDRLVAELK 191 Query: 191 NAADKAYELDLQMERLHREIKEIKDAQGDDTDVELRRIMINNQQFNLPANFIK 243 AA ELD +ME +H+EI +IK+ D++ + + I INN++F LP +F++ Sbjct: 192 EAATSVSELDKKMENIHKEISQIKERI--DSNDDPQEISINNERFYLPHDFLQ 242 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,345,536,224 Number of extensions: 52194552 Number of successful extensions: 167516 Number of sequences better than 1.0e-05: 746 Number of HSP's gapped: 168501 Number of HSP's successfully gapped: 746 Length of query: 247 Length of database: 3,060,696,203 Length adjustment: 132 Effective length of query: 115 Effective length of database: 1,817,122,223 Effective search space: 208969055645 Effective search space used: 208969055645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)