BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0059.p (322 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase 318 3e-85 B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase 271 9e-71 A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase 186 2e-45 B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase 186 2e-45 C5D333.1 C5D333_9BACI Metal dependent phosphohydrolase 174 7e-42 >B3KBK2.1 B3KBK2_9BACI Metal dependent phophohydrolase Length = 348 Score = 318 bits (816), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 162/326 (49%), Positives = 221/326 (67%), Gaps = 5/326 (1%) Query: 2 GLHVLPKRTVVQREEITILLRHKIEYVDIEPRTASHAED--SGLGHSLND---NFDHAIQ 56 G+HVL K +++ E+I+ LL+H I+Y++I+PR S + D S + S N +F+ A Sbjct: 23 GVHVLQKGALLRSEDISKLLQHGIDYIEIQPRALSVSLDPLSEISDSFNKVKPHFNLAFD 82 Query: 57 NYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLLERDDIDTYHHSLQVGL 116 ++F EA G F +S+VDD QPL+ +L+ DVVSLLLL D TY HS+QVG+ Sbjct: 83 GCSALFTEASQSGKFNESVVDDVFQPLVGSLNEHTDVVSLLLLFNGTDDYTYRHSIQVGM 142 Query: 117 LSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEEEELKRHTTFGY 176 LSYYIA WMG+S +E YEI +AGYLHDIGKS++ IL+K G LTP E EE+K HT +GY Sbjct: 143 LSYYIADWMGYSGKEAYEIGKAGYLHDIGKSKISRDILDKPGKLTPEEFEEVKLHTIYGY 202 Query: 177 EIIRGSKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEIVTVANIYMGLTTSRLNQ 236 E+I S D+ TA+VALQHHE +D +GYP + IHPY+ I VA++Y +TT+R+ Q Sbjct: 203 EMIAESMDDKVTAMVALQHHERDDRSGYPRALHADQIHPYSHICAVADVYSAMTTNRVYQ 262 Query: 237 PKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEIGTIVMNNPLDMF 296 KQ L+ VLR+++ + FGKLNG A +LP F+GK V L+ GE G IVMN+P D F Sbjct: 263 SKQQLLTVLRELYSLSFGKLNGKPTHAFIQQMLPNFIGKKVLLTTGESGIIVMNHPSDYF 322 Query: 297 RPLVKVDNVFKDLSRERSLSVEEIIM 322 RPLVK D+ F DLS+E ++EI++ Sbjct: 323 RPLVKTDDRFIDLSKEYHTEIQEILL 348 >B1DKG5.1 B1DKG5_9BACL Metal dependent phosphohydrolase Length = 354 Score = 271 bits (692), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 138/332 (41%), Positives = 212/332 (63%), Gaps = 11/332 (3%) Query: 2 GLHVLPKRTVVQREEITILLRHKIEYVDIEPR-TASHAEDSGLGHSLND--------NFD 52 GLHVL K TV+ +++I L +H+++YVDIE R T L ++N +++ Sbjct: 23 GLHVLSKGTVLAQKDIAKLFQHQVDYVDIEDRQTTEQPIHRTLETTVNPKWLPNVRPDYE 82 Query: 53 HAIQNYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLLERDDIDTYHHSL 112 +A++ ++ +F +A G Q V QPLL+ ++DVVS+LLLL D TY HS+ Sbjct: 83 NAVKGFQGLFQKATDGGVVKQDEVAAAFQPLLKNFQMERDVVSMLLLLNTADDYTYQHSV 142 Query: 113 QVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEEEELKRHT 172 QVG+LSYY+A+W+G+++ E EIS++GYLHDIGKS++P SILNK LT E EE+K+HT Sbjct: 143 QVGMLSYYLASWLGYTEMEALEISKSGYLHDIGKSRIPSSILNKPAKLTDEEFEEVKKHT 202 Query: 173 TFGYEIIRGSKMD--EKTALVALQHHEFEDGTGYPNQILKSGIHPYTEIVTVANIYMGLT 230 +GY+II+ S D E A+ ALQHHE DG+GYP+ + IHP +++ V +IY + Sbjct: 203 LYGYDIIQQSFEDKSEIMAVSALQHHERNDGSGYPHGLRGDEIHPVAKLIAVVDIYSAMI 262 Query: 231 TSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEIGTIVMN 290 + R+ + K+ L VL+++++M F +L+ H++P F+GK L +GE+GTIVM Sbjct: 263 SERVYKKKRDLFFVLKELYKMSFNELDAHTTHTFIKHMIPNFIGKKAVLESGEVGTIVMT 322 Query: 291 NPLDMFRPLVKVDNVFKDLSRERSLSVEEIIM 322 +P + FRPL++ F DL+ +R ++EII Sbjct: 323 HPTEFFRPLIQTGERFIDLTVDREYEIKEIIF 354 >A3DDV3.1 A3DDV3_CLOTH Metal dependent phosphohydrolase Length = 345 Score = 186 bits (472), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 95/282 (33%), Positives = 160/282 (56%), Gaps = 1/282 (0%) Query: 41 SGLGHSLNDNFDHAIQNYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLL 100 S + S N +++ ++ ++SI +A VD + ++ LD ++D+V + + Sbjct: 64 SEISESFNVSYNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQI 123 Query: 101 ERDDIDTYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGIL 160 + D TY H + V LL + W+G+SK+ ++ +AG LHDIGK++VP ILNK L Sbjct: 124 KSADQYTYTHCVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKL 183 Query: 161 TPAEEEELKRHTTFGYEII-RGSKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEI 219 TP E EE+K+HT +GY I+ + ++D A+ L HHE DG+GYP I+ + Sbjct: 184 TPEEYEEIKKHTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARV 243 Query: 220 VTVANIYMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQL 279 + VA+IY +T++R+ + K+ V + E GKL+ VV A ++ ++G +L Sbjct: 244 IAVADIYDAMTSNRVYREKESPFEVFHIIEEEAVGKLDVRVVNAFLSNIAAYYIGDFAKL 303 Query: 280 SNGEIGTIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEII 321 + GEI IV NP + RP+++VD+ + DL+ E+ L + EI+ Sbjct: 304 NTGEICEIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEIL 345 >B4BFA5.1 B4BFA5_CLOTM Metal dependent phosphohydrolase Length = 345 Score = 186 bits (472), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 95/282 (33%), Positives = 160/282 (56%), Gaps = 1/282 (0%) Query: 41 SGLGHSLNDNFDHAIQNYESIFLEALAQGTFTQSMVDDTLQPLLETLDTQKDVVSLLLLL 100 S + S N +++ ++ ++SI +A VD + ++ LD ++D+V + + Sbjct: 64 SEISESFNVSYNASVNCFKSILYDAAVGKNINMEKVDKVVMSVVARLDEKRDIVRCINQI 123 Query: 101 ERDDIDTYHHSLQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGIL 160 + D TY H + V LL + W+G+SK+ ++ +AG LHDIGK++VP ILNK L Sbjct: 124 KSADQYTYTHCVNVSLLCMLLGKWLGYSKDSLIKLVQAGLLHDIGKAKVPKEILNKPSKL 183 Query: 161 TPAEEEELKRHTTFGYEII-RGSKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEI 219 TP E EE+K+HT +GY I+ + ++D A+ L HHE DG+GYP I+ + Sbjct: 184 TPEEYEEIKKHTVYGYRILEKEHEIDRDVAIAVLMHHERMDGSGYPTGAKSDQINMMARV 243 Query: 220 VTVANIYMGLTTSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQL 279 + VA+IY +T++R+ + K+ V + E GKL+ VV A ++ ++G +L Sbjct: 244 IAVADIYDAMTSNRVYREKESPFEVFHIIEEEAVGKLDVRVVNAFLSNIAAYYIGDFAKL 303 Query: 280 SNGEIGTIVMNNPLDMFRPLVKVDNVFKDLSRERSLSVEEII 321 + GEI IV NP + RP+++VD+ + DL+ E+ L + EI+ Sbjct: 304 NTGEICEIVYINPRYVSRPIIRVDDQYIDLTVEKDLKILEIL 345 >C5D333.1 C5D333_9BACI Metal dependent phosphohydrolase Length = 359 Score = 174 bits (442), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 116/333 (34%), Positives = 175/333 (52%), Gaps = 17/333 (5%) Query: 5 VLPKRTVVQREEITILLRHKIEYVDIEPRTAS---HAEDSGLGHSLNDN---------FD 52 ++PK TV+ + +L + IE V +EP A+ LG DN + Sbjct: 27 IMPKNTVLTPALLNVLEKFLIEEVCVEPVLANGDPFRPAELLGQHDGDNTSNEQPISLYL 86 Query: 53 HAIQNYESIFLEALAQGTFTQSMVDDTLQPLLETL-DTQKDVVSLLLLLERDDIDTYHHS 111 A+Q Y+ F + + D + PL + +K++++L ++D YHH+ Sbjct: 87 KAVQKYKKAFQSWQSGSPIDIGEIRDIIIPLFDKFVHHRKELLTLYRYATKEDY-LYHHA 145 Query: 112 LQVGLLSYYIAAWMGHSKEERYEISRAGYLHDIGKSQVPLSILNKAGILTPAEEEELKRH 171 + VGLLS ++A + +SK + Y+I AG L D G ++V IL K LT +E EE+K+H Sbjct: 146 VSVGLLSGFLAKQLDYSKGDCYQIVLAGILSDCGMAKVDQRILAKKSALTTSEYEEVKQH 205 Query: 172 TTFGYEIIRG-SKMDEKTALVALQHHEFEDGTGYPNQILKSGIHPYTEIVTVANIYMGLT 230 T FGY +++ + + E L LQHHE DGTGYP I IHPY++IV VA++Y +T Sbjct: 206 TVFGYRMVQKITSLKEGVKLAVLQHHERNDGTGYPFGIRDRQIHPYSKIVAVADVYHAMT 265 Query: 231 TSRLNQPKQGLVAVLRKVHEMGFGKLNGTVVQALTGHLLPGFVGKNVQLSNGEIGTIVMN 290 + RL Q KQ L K+ FGKL+ +Q L L+ +G V+LSNGE+ IV Sbjct: 266 SERLYQRKQSPFKALEKIQVEHFGKLSMEAIQILLDSLINFHIGTVVKLSNGEMAEIVFM 325 Query: 291 NPLDMFRPLVKV--DNVFKDLSRERSLSVEEII 321 RP+VK+ + F LS R + +EE+I Sbjct: 326 QREAPTRPIVKIVDKDEFLSLSEHRDIHIEEVI 358 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,912,458,374 Number of extensions: 80626936 Number of successful extensions: 195898 Number of sequences better than 1.0e-05: 2176 Number of HSP's gapped: 194476 Number of HSP's successfully gapped: 2176 Length of query: 322 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 186 Effective length of database: 1,779,438,163 Effective search space: 330975498318 Effective search space used: 330975498318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)