BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0053.p (299 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GI05.1 B7GI05_ANOFW Dehydrogenase 292 3e-77 C5D251.1 C5D251_9BACI Short-chain dehydrogenase/reductase SDR 283 1e-74 C0Z583.1 C0Z583_BREBN Putative oxidoreductase 266 2e-69 B9J308.1 B9J308_BACCQ Oxidoreductase, short-chain dehydrogenase/... 254 8e-66 B7HTK8.1 B7HTK8_BACC7 Oxidoreductase, short-chain dehydrogenase/... 254 8e-66 >B7GI05.1 B7GI05_ANOFW Dehydrogenase Length = 316 Score = 292 bits (747), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 142/279 (50%), Positives = 191/279 (68%) Query: 19 NADTIALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVIS 78 N+ +AL+TG SSGFGLL ++ALAR+G RV A+MR++ + L A EAG+ +RI VIS Sbjct: 37 NSPPVALVTGASSGFGLLVSVALAREGYRVIASMRNMQNKEMLATVAREAGVYDRIEVIS 96 Query: 79 LDVTDSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEV 138 LDVTD A+++S V V +++GR+DVLVNN W RQ TNFFGL+ V Sbjct: 97 LDVTDFAAVESVVNDVSNRYGRIDVLVNNAGFVVGGFVEELTLEEWERQFATNFFGLVAV 156 Query: 139 TKAVLPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVV 198 TKAVLP+MR QR+G I+NISS+SG GFP PY ASK+AVEGFSESLR E+LP+G+ VV Sbjct: 157 TKAVLPIMRTQRSGKIINISSISGRVGFPAMGPYVASKFAVEGFSESLRLEMLPYGVYVV 216 Query: 199 LVEPASFRTPIWDKGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITA 258 L+EP SF+T IW KG++G+ SPY++++ +++Y A+TAP P++V I ++ Sbjct: 217 LIEPGSFKTNIWSKGLQGVSVRSGSPYEKEMKTIMQYVNRVAKTAPPPDEVIRQIIKVVQ 276 Query: 259 MKAPRLRYPVGKGSRLLTIGKTLLPWKVFEGIIASSLKK 297 P LRYPVG+G L K LPWK +E I+ SLK+ Sbjct: 277 SPTPHLRYPVGRGVTLTLRLKNWLPWKWWEWIVMKSLKQ 315 >C5D251.1 C5D251_9BACI Short-chain dehydrogenase/reductase SDR Length = 286 Score = 283 bits (724), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 134/277 (48%), Positives = 190/277 (68%) Query: 23 IALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVT 82 +A +TG SSGFGLLT++ LA +G RV A+MR+++ +G L A EAG+ +RI ++ LDVT Sbjct: 10 VAFVTGASSGFGLLTSVVLAGEGYRVLASMRNLNNKGRLEAAAKEAGVTDRIEIVQLDVT 69 Query: 83 DSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAV 142 D +++++ + V+ ++GR+D+LVNN WRRQ +TNFFGL+ VTKAV Sbjct: 70 DFSAVETVIQDVIRRYGRIDLLVNNAGYAAGGFTEELAVEEWRRQFETNFFGLVAVTKAV 129 Query: 143 LPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVEP 202 LP MR +R+G IVNISS+SG GFP PY ASK+AVEGFSESLR E+LP+G+ VVL+EP Sbjct: 130 LPSMRERRSGKIVNISSISGRIGFPSMGPYVASKFAVEGFSESLRLEMLPYGVHVVLIEP 189 Query: 203 ASFRTPIWDKGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITAMKAP 262 S++T IW KG+ + NSPY +++ +L+Y A+TAP P++V I ++ P Sbjct: 190 GSYKTDIWSKGLGAVTIHPNSPYAKEMKAILKYVNQVADTAPAPDEVIRQIVQVAKSPYP 249 Query: 263 RLRYPVGKGSRLLTIGKTLLPWKVFEGIIASSLKKMK 299 +LRYPVGKG +L + K +LPWK +E I+ L + K Sbjct: 250 KLRYPVGKGVKLGIVLKHVLPWKWWERIVTKRLWEGK 286 >C0Z583.1 C0Z583_BREBN Putative oxidoreductase Length = 278 Score = 266 bits (679), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 132/269 (49%), Positives = 174/269 (64%) Query: 23 IALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVT 82 IAL+TGTSSGFG ++AL + G +V A MRD +++ L + A + + VISLDVT Sbjct: 6 IALVTGTSSGFGKHASVALVKAGFQVIAAMRDPAKRDPLDKLASLLIDPDHLEVISLDVT 65 Query: 83 DSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAV 142 +S IQ A+ +++ K GR+D+LVNN WR+Q D N FGLI+VT+AV Sbjct: 66 NSEQIQEAITSIIAKHGRIDLLVNNAGYALGGFAEEVSSEEWRKQFDVNVFGLIDVTRAV 125 Query: 143 LPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVEP 202 LP MR+Q+AG I+N+SS+SG GFPG +PYAASK+AVEGFSESLR E+LPF ++VVLVEP Sbjct: 126 LPYMRQQQAGRIINVSSISGRFGFPGLSPYAASKHAVEGFSESLRLEMLPFQVQVVLVEP 185 Query: 203 ASFRTPIWDKGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITAMKAP 262 SFRT IW+KGM+ +SPY Q+ Q++R+ E AP P+ V I P Sbjct: 186 GSFRTAIWEKGMQDQQLDEDSPYASQMKQLIRHVEAIIEKAPAPDAVISTIVHAATTPKP 245 Query: 263 RLRYPVGKGSRLLTIGKTLLPWKVFEGII 291 R RYPVG+G L + K LPW E I+ Sbjct: 246 RFRYPVGRGVGLTIVAKNWLPWSWIERIV 274 >B9J308.1 B9J308_BACCQ Oxidoreductase, short-chain dehydrogenase/reductase Length = 291 Score = 254 bits (648), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 125/278 (44%), Positives = 175/278 (62%), Gaps = 3/278 (1%) Query: 23 IALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVT 82 IA+ITG SSGFGLLT L LA++ V ATMR++ +QG LL Q + L + I + LDVT Sbjct: 15 IAVITGASSGFGLLTTLELAKKDYLVIATMRNLEKQGNLLSQVTQLNLQQNIKIQQLDVT 74 Query: 83 DSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAV 142 D S+ + VL + +VD+L+NN +R+Q +TNFFG I +T+ V Sbjct: 75 DQNSLHN-FQLVLKEINKVDLLINNAGYANGGFVEEIPVEEYRKQFETNFFGAISITQLV 133 Query: 143 LPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVEP 202 LP MR Q++G I+NISS+SG GFPG +PY +SKYA+EG+SESLR EV PFGI V LVEP Sbjct: 134 LPYMREQKSGKIINISSISGQVGFPGLSPYVSSKYALEGWSESLRLEVKPFGINVALVEP 193 Query: 203 ASFRTPIWD--KGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITAMK 260 S+ T IW+ K + H+ SPY+E + ++ ++ ++T +P VA I I K Sbjct: 194 GSYNTNIWEVGKQLAENHSETTSPYKEYMDKIQKHINSGSDTFGNPIDVANKIVEIAESK 253 Query: 261 APRLRYPVGKGSRLLTIGKTLLPWKVFEGIIASSLKKM 298 LRYP+GKG + + K +LPW+++E ++ S KKM Sbjct: 254 RTNLRYPIGKGVKFMIFAKKILPWRLWEYLVLRSFKKM 291 >B7HTK8.1 B7HTK8_BACC7 Oxidoreductase, short-chain dehydrogenase/reductase family Length = 291 Score = 254 bits (648), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 125/278 (44%), Positives = 175/278 (62%), Gaps = 3/278 (1%) Query: 23 IALITGTSSGFGLLTALALARQGCRVAATMRDISRQGELLRQAGEAGLAERIHVISLDVT 82 IA+ITG SSGFGLLT L LA++ V ATMR++ +QG LL Q + L + I + LDVT Sbjct: 15 IAVITGASSGFGLLTTLELAKKDYLVIATMRNLEKQGNLLSQVTQLNLQQNIKIQQLDVT 74 Query: 83 DSASIQSAVAAVLDKFGRVDVLVNNXXXXXXXXXXXXXXXXWRRQMDTNFFGLIEVTKAV 142 D S+ + VL + +VD+L+NN +R+Q +TNFFG I +T+ V Sbjct: 75 DQNSLHN-FQLVLKEINKVDLLINNAGYANGGFVEEIPVEEYRKQFETNFFGAISITQLV 133 Query: 143 LPVMRRQRAGLIVNISSVSGLTGFPGYAPYAASKYAVEGFSESLRQEVLPFGIRVVLVEP 202 LP MR Q++G I+NISS+SG GFPG +PY +SKYA+EG+SESLR EV PFGI V LVEP Sbjct: 134 LPYMREQKSGKIINISSISGQVGFPGLSPYVSSKYALEGWSESLRLEVKPFGINVALVEP 193 Query: 203 ASFRTPIWD--KGMEGMHTSVNSPYQEQLAQVLRYSRMSAETAPDPEQVAGLIARITAMK 260 S+ T IW+ K + H+ SPY+E + ++ ++ ++T +P VA I I K Sbjct: 194 GSYNTNIWEVGKQLAENHSETTSPYKEYMDKIQKHINSGSDTFGNPIDVANKIVEIAESK 253 Query: 261 APRLRYPVGKGSRLLTIGKTLLPWKVFEGIIASSLKKM 298 LRYP+GKG + + K +LPW+++E ++ S KKM Sbjct: 254 RTNLRYPIGKGVKFMIFAKKILPWRLWEYLVLRSFKKM 291 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,526,854,862 Number of extensions: 56174006 Number of successful extensions: 249639 Number of sequences better than 1.0e-05: 26604 Number of HSP's gapped: 211817 Number of HSP's successfully gapped: 26675 Length of query: 299 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 164 Effective length of database: 1,788,859,178 Effective search space: 293372905192 Effective search space used: 293372905192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)