BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0049.p (327 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZKH5.1 C0ZKH5_BREBN Putative uncharacterized protein 347 1e-93 C2PH93.1 C2PH93_BACCE 3-beta hydroxysteroid dehydrogenase/isomerase 314 9e-84 B7JF35.1 B7JF35_BACC0 3-beta hydroxysteroid dehydrogenase/isomer... 309 2e-82 B3YMJ5.1 B3YMJ5_BACCE 3-beta hydroxysteroid dehydrogenase/isomer... 309 2e-82 C3GL40.1 C3GL40_BACTU 3-beta hydroxysteroid dehydrogenase/isomerase 309 2e-82 >C0ZKH5.1 C0ZKH5_BREBN Putative uncharacterized protein Length = 333 Score = 347 bits (889), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 168/326 (51%), Positives = 220/326 (67%) Query: 1 VLVTGATGFLGRHAVQRFAELGWEVFGLGRNLEAGQQIEKDGAVFVCEDLRHTEAAARAC 60 VLVTGATGFLG+ R E+G++V GR+ GQ +++ G F+ DLR EA +AC Sbjct: 5 VLVTGATGFLGQKLATRLHEMGYDVTAQGRDERIGQLLQERGIRFLRADLRDREAMVKAC 64 Query: 61 TGMDLVFHCAALSSPWGKYSDFVACNVEATRNIAEGCKRHGVPRLIHISTPSLYSSRKSR 120 D+V H AA SSPWG Y D NV T ++ EGCK HG+ RL+H+STPS+Y + + Sbjct: 65 RDQDIVHHAAAFSSPWGTYRDMYETNVTGTIHVIEGCKLHGIQRLVHVSTPSIYFAFDDK 124 Query: 121 LSIREAEPLPPKPANSYAATKLMAERVVQQAAGEGLAVFILRPRAIFGPMDNALFPRLLA 180 L IRE EP+P + AN+YA TK AE V +A GL +RPRA+FGP DNA+ PRL+ Sbjct: 125 LGIREDEPMPVRFANTYAQTKYQAELEVDKAFLAGLPTITIRPRALFGPGDNAILPRLIR 184 Query: 181 ANQGKGVPLLDGGTAMIDVTCVENVIDAMLLCSSAPLSAAGRAYNITNGEPILFRDLLER 240 AN+ K VPL+DGG A+ID+T VENV+DA++LC +P G+AYNITNGEP+ ++L Sbjct: 185 ANEKKFVPLIDGGKAIIDLTYVENVVDALILCMDSPAHTLGQAYNITNGEPVTMIEVLSD 244 Query: 241 LFARLEMPLHSRQLPYRTAYALAGLLEGVYKLLPFLGEPVLTRYSASALGVSQTLDISLA 300 +F RL +PL +R+LPY AYA A +LE + K + EPVLTRYS L SQTLDIS A Sbjct: 245 VFRRLGVPLKTRELPYWKAYAAAWVLETLSKTVLGYREPVLTRYSVGVLAKSQTLDISKA 304 Query: 301 REQLGYHPRVTTDEGLQSFAEWWREQ 326 + LGY PRV+ +G+++F EWWR Q Sbjct: 305 KRDLGYEPRVSIAQGIETFTEWWRTQ 330 >C2PH93.1 C2PH93_BACCE 3-beta hydroxysteroid dehydrogenase/isomerase Length = 326 Score = 314 bits (804), Expect = 9e-84, Method: Compositional matrix adjust. Identities = 142/326 (43%), Positives = 208/326 (63%) Query: 1 VLVTGATGFLGRHAVQRFAELGWEVFGLGRNLEAGQQIEKDGAVFVCEDLRHTEAAARAC 60 +LVTG TGFLG+ R +G+EV GRN G+ +E++G FV L + + C Sbjct: 1 MLVTGGTGFLGQKLAFRLKNMGYEVTATGRNKTIGKVLEQNGIEFVHCSLEDRDRVLQVC 60 Query: 61 TGMDLVFHCAALSSPWGKYSDFVACNVEATRNIAEGCKRHGVPRLIHISTPSLYSSRKSR 120 G D +FH ALSSPWGKY DF NV T++I EGC+++G+ RLIH+STPS+Y R Sbjct: 61 KGKDYIFHSGALSSPWGKYEDFYNANVLGTKHIIEGCQKYGIKRLIHVSTPSIYFYYDER 120 Query: 121 LSIREAEPLPPKPANSYAATKLMAERVVQQAAGEGLAVFILRPRAIFGPMDNALFPRLLA 180 ++ E LP N YA TK MAE+ + QA GL V +RPRA+FGP DNA+ PRL+ Sbjct: 121 QNVVENAKLPDTFVNHYATTKHMAEQAIDQAFAHGLPVITIRPRALFGPGDNAILPRLIK 180 Query: 181 ANQGKGVPLLDGGTAMIDVTCVENVIDAMLLCSSAPLSAAGRAYNITNGEPILFRDLLER 240 + +P + ++D+T VENV+DA+LLC +P G+ YNITN E + +++E Sbjct: 181 VCEKGALPRIGTEDVLVDITYVENVVDALLLCMHSPKHTLGQKYNITNDERVNLYEVIEN 240 Query: 241 LFARLEMPLHSRQLPYRTAYALAGLLEGVYKLLPFLGEPVLTRYSASALGVSQTLDISLA 300 + RL+ + +++ Y+TA++LA +LEG+ K + F EP+LT+Y+ S L SQTL+I A Sbjct: 241 VMKRLDKEVKYKKISYKTAFSLAAILEGISKTILFGKEPILTKYTVSVLSKSQTLNIDKA 300 Query: 301 REQLGYHPRVTTDEGLQSFAEWWREQ 326 +E+LGY P+++ +EG+ F +WW+ Q Sbjct: 301 KEELGYAPKISIEEGITKFVDWWKIQ 326 >B7JF35.1 B7JF35_BACC0 3-beta hydroxysteroid dehydrogenase/isomerase family protein Length = 328 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 143/326 (43%), Positives = 204/326 (62%) Query: 1 VLVTGATGFLGRHAVQRFAELGWEVFGLGRNLEAGQQIEKDGAVFVCEDLRHTEAAARAC 60 +LVTG TGFLG+ R +G+EV GRN G+ +E++G FV L E + C Sbjct: 3 MLVTGGTGFLGQKLAFRLKNMGYEVTATGRNKTIGKVLEQNGIKFVHCPLEDRERVLQVC 62 Query: 61 TGMDLVFHCAALSSPWGKYSDFVACNVEATRNIAEGCKRHGVPRLIHISTPSLYSSRKSR 120 D +FH ALSSPWGKY DF NV T++I EG +++G+ RLIH+STPS+Y R Sbjct: 63 KDKDYIFHSGALSSPWGKYEDFYKANVLGTKHIIEGSQKYGIKRLIHVSTPSIYFYYDER 122 Query: 121 LSIREAEPLPPKPANSYAATKLMAERVVQQAAGEGLAVFILRPRAIFGPMDNALFPRLLA 180 ++ E LP N YA TK MAE+ + QA GL V +RPRA+FGP DNA+ PRL+ Sbjct: 123 QNVVENAKLPDTFVNHYATTKYMAEQAIDQAFAHGLPVITIRPRALFGPGDNAILPRLIK 182 Query: 181 ANQGKGVPLLDGGTAMIDVTCVENVIDAMLLCSSAPLSAAGRAYNITNGEPILFRDLLER 240 + +P + ++D+T VENV+DA+LLC +P G+ YNITN E I +++E Sbjct: 183 VCEKGALPRIGTENVLVDITYVENVVDALLLCMHSPKHTLGQKYNITNDERINLYEVIEN 242 Query: 241 LFARLEMPLHSRQLPYRTAYALAGLLEGVYKLLPFLGEPVLTRYSASALGVSQTLDISLA 300 + RL+ + +++ Y+TA+ LA +LEG+ K + F EP+LT+Y+ S L SQTL I A Sbjct: 243 VMKRLDKEVRYKKISYKTAFTLAAILEGISKTILFGKEPILTKYTVSVLSKSQTLSIDKA 302 Query: 301 REQLGYHPRVTTDEGLQSFAEWWREQ 326 +E+LGY P+V+ +EG+ F +WW+ Q Sbjct: 303 KEELGYAPKVSIEEGITKFVDWWKIQ 328 >B3YMJ5.1 B3YMJ5_BACCE 3-beta hydroxysteroid dehydrogenase/isomerase family protein Length = 328 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 143/326 (43%), Positives = 204/326 (62%) Query: 1 VLVTGATGFLGRHAVQRFAELGWEVFGLGRNLEAGQQIEKDGAVFVCEDLRHTEAAARAC 60 +LVTG TGFLG+ R +G+EV GRN G+ +E++G FV L E + C Sbjct: 3 MLVTGGTGFLGQKLAFRLKNMGYEVTATGRNKTIGKVLEQNGIKFVHCPLEDRERVLQVC 62 Query: 61 TGMDLVFHCAALSSPWGKYSDFVACNVEATRNIAEGCKRHGVPRLIHISTPSLYSSRKSR 120 D +FH ALSSPWGKY DF NV T++I EG +++G+ RLIH+STPS+Y R Sbjct: 63 KDKDYIFHSGALSSPWGKYEDFYNANVLGTKHIIEGSQKYGIKRLIHVSTPSIYFYYDER 122 Query: 121 LSIREAEPLPPKPANSYAATKLMAERVVQQAAGEGLAVFILRPRAIFGPMDNALFPRLLA 180 ++ E LP N YA TK MAE+ + QA GL V +RPRA+FGP DNA+ PRL+ Sbjct: 123 QNVVENAKLPDTFVNHYATTKYMAEQAIDQAFAHGLPVITIRPRALFGPGDNAILPRLIK 182 Query: 181 ANQGKGVPLLDGGTAMIDVTCVENVIDAMLLCSSAPLSAAGRAYNITNGEPILFRDLLER 240 + +P + ++D+T VENV+DA+LLC +P G+ YNITN E I +++E Sbjct: 183 VCEKGALPRIGTENVLVDITYVENVVDALLLCMHSPKHTLGQKYNITNDERINLYEVIEN 242 Query: 241 LFARLEMPLHSRQLPYRTAYALAGLLEGVYKLLPFLGEPVLTRYSASALGVSQTLDISLA 300 + RL+ + +++ Y+TA+ LA +LEG+ K + F EP+LT+Y+ S L SQTL I A Sbjct: 243 VMKRLDKEVRYKKISYKTAFTLAAILEGISKTILFGKEPILTKYTVSVLSKSQTLSIDKA 302 Query: 301 REQLGYHPRVTTDEGLQSFAEWWREQ 326 +E+LGY P+V+ +EG+ F +WW+ Q Sbjct: 303 KEELGYAPKVSIEEGITKFVDWWKIQ 328 >C3GL40.1 C3GL40_BACTU 3-beta hydroxysteroid dehydrogenase/isomerase Length = 326 Score = 309 bits (792), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 143/326 (43%), Positives = 204/326 (62%) Query: 1 VLVTGATGFLGRHAVQRFAELGWEVFGLGRNLEAGQQIEKDGAVFVCEDLRHTEAAARAC 60 +LVTG TGFLG+ R +G+EV GRN G+ +E++G FV L E + C Sbjct: 1 MLVTGGTGFLGQKLAFRLKNMGYEVTATGRNKTIGKVLEQNGIKFVHCPLEDRERVLQVC 60 Query: 61 TGMDLVFHCAALSSPWGKYSDFVACNVEATRNIAEGCKRHGVPRLIHISTPSLYSSRKSR 120 D +FH ALSSPWGKY DF NV T++I EG +++G+ RLIH+STPS+Y R Sbjct: 61 KDKDYIFHSGALSSPWGKYEDFYNANVLGTKHIIEGSQKYGIKRLIHVSTPSIYFYYDER 120 Query: 121 LSIREAEPLPPKPANSYAATKLMAERVVQQAAGEGLAVFILRPRAIFGPMDNALFPRLLA 180 ++ E LP N YA TK MAE+ + QA GL V +RPRA+FGP DNA+ PRL+ Sbjct: 121 QNVVENAKLPDTFVNHYATTKYMAEQAIDQAFAHGLPVITIRPRALFGPGDNAILPRLIK 180 Query: 181 ANQGKGVPLLDGGTAMIDVTCVENVIDAMLLCSSAPLSAAGRAYNITNGEPILFRDLLER 240 + +P + ++D+T VENV+DA+LLC +P G+ YNITN E I +++E Sbjct: 181 VCEKGALPRIGTENVLVDITYVENVVDALLLCMHSPKHTLGQKYNITNDERINLYEVIEN 240 Query: 241 LFARLEMPLHSRQLPYRTAYALAGLLEGVYKLLPFLGEPVLTRYSASALGVSQTLDISLA 300 + RL+ + +++ Y+TA+ LA +LEG+ K + F EP+LT+Y+ S L SQTL I A Sbjct: 241 VMKRLDKEVRYKKISYKTAFTLAAILEGISKTILFGKEPILTKYTVSVLSKSQTLSIDKA 300 Query: 301 REQLGYHPRVTTDEGLQSFAEWWREQ 326 +E+LGY P+V+ +EG+ F +WW+ Q Sbjct: 301 KEELGYAPKVSIEEGITKFVDWWKIQ 326 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,924,892,029 Number of extensions: 79683703 Number of successful extensions: 233441 Number of sequences better than 1.0e-05: 2906 Number of HSP's gapped: 232106 Number of HSP's successfully gapped: 2918 Length of query: 327 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 191 Effective length of database: 1,779,438,163 Effective search space: 339872689133 Effective search space used: 339872689133 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)