BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0027.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFW4.1 B3KFW4_9BACI Acetyl xylan esterase 402 e-110 C0D680.1 C0D680_9CLOT Putative uncharacterized protein 184 7e-45 A6WFR1.1 A6WFR1_KINRD Acetyl xylan esterase 159 5e-37 B1DK58.1 B1DK58_9BACL Acetyl xylan esterase 158 5e-37 B4AIZ3.1 B4AIZ3_BACPU Cephalosporin-C deacetylase 157 1e-36 >B3KFW4.1 B3KFW4_9BACI Acetyl xylan esterase Length = 321 Score = 402 bits (1032), Expect = e-110, Method: Compositional matrix adjust. Identities = 179/316 (56%), Positives = 238/316 (75%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 MNTIE RK+EL N RPEPT+++ V+N FW+G+L + +PL ++S PE TPYP M V +V Sbjct: 1 MNTIEQRKKELGNLRPEPTIDEQVLNIFWEGVLKSHGDRPLRMVSRPEATPYPHMEVFRV 60 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAVD 120 ++ +D+T ++AW+I PA + G PCI+TFPGYTGDRG+PERYA WL+LGY+V AVD Sbjct: 61 TYHGYDDTPINAWYIAPANPSSKAGARPCIVTFPGYTGDRGFPERYAHWLMLGYTVFAVD 120 Query: 121 VRGQLGETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPSRX 180 VRGQ GETGNLLP++SGV KGW++QG+ + SYYMA+AMD VRAV+ A++ GVDP++ Sbjct: 121 VRGQGGETGNLLPLDSGVVKGWVSQGITDIHRSYYMAIAMDAVRAVDAASRQPGVDPAKI 180 Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGVLNSTSSLTEIGAYVRRYPEHL 240 + ADIPN+CR+D+G+L S SSL EI Y++RYPE L Sbjct: 181 AVVGGSQGGGLSMLAGALNPKVAAIVADIPNMCRMDYGILYSVSSLNEIAQYIKRYPEQL 240 Query: 241 ERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDYPFSGH 300 +++L LA+FD++NLAPRI AP+++SVGWKDTVCMPE++YAAYNRIE+ K+I DYPFSGH Sbjct: 241 DQILAHLAHFDMLNLAPRIQAPIMVSVGWKDTVCMPETIYAAYNRIEAAKEIRDYPFSGH 300 Query: 301 EVSEYHLRQKVLFLQK 316 EVSEY R+ VLFL++ Sbjct: 301 EVSEYQKREGVLFLER 316 >C0D680.1 C0D680_9CLOT Putative uncharacterized protein Length = 320 Score = 184 bits (468), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 110/318 (34%), Positives = 162/318 (50%), Gaps = 5/318 (1%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M IE R +ELE RPE T + EFW+ L+E + L + P RV + Sbjct: 1 MRYIEERLRELEAYRPELT-RRPDFGEFWENTLSESHDRELRPTAKQVDYPCGHARVYDI 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAVD 120 S+ FD TR+H W + PA G PC+I + G+T RG P + W++ G +VLAVD Sbjct: 60 SYDGFDGTRIHGWFLVPAFGK--AGRWPCLIQYHGFTDSRGLPWQLMHWVMAGMAVLAVD 117 Query: 121 VRGQLGETGNLLP-MESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPSR 179 R Q G TGN +SG+ T G+L+ YY AV MD VRAV+ AA VDP R Sbjct: 118 CRAQGGVTGNCAHYTDSGMVANVTTLGILDPHEYYYRAVYMDCVRAVDFAAGRPEVDPGR 177 Query: 180 XXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGVLNSTSSLTEIGAYVRRYPEH 239 ++P+ ++ + S + ++ RYP+ Sbjct: 178 IAVRGTSQGGALGMAVCALDGRPALGIVNVPSNSDIETRIAGRHGSFSAAADFLMRYPDQ 237 Query: 240 LERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDYPFSG 299 +RVL+TL+YFD +N+A RI P+ SV D VC + YA+YNRI + K+I YPF+G Sbjct: 238 TDRVLETLSYFDTMNMADRIRCPIFASVALGDQVCPAKCYYASYNRITAPKEITVYPFNG 297 Query: 300 HE-VSEYHLRQKVLFLQK 316 H+ + H+ +++ +L++ Sbjct: 298 HDGAGDAHMERELGYLRE 315 >A6WFR1.1 A6WFR1_KINRD Acetyl xylan esterase Length = 328 Score = 159 bits (401), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 107/319 (33%), Positives = 159/319 (49%), Gaps = 12/319 (3%) Query: 1 MNTIELRKQELENCRPEPTL-EQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDK 59 M +L ++LE + TL E ++EFW L+ PL++ P VT +R + Sbjct: 1 MALSDLSLEDLEKL--DLTLHEPAGLDEFWARTLSAADAHPLDVHREPVVTHLTTLRHED 58 Query: 60 VSFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSVLAV 119 +SF +D R++AW + PA A GPLP ++ F GY G RG P+ +W GY+ L V Sbjct: 59 LSFAGYDGHRINAWLVLPAGAE---GPLPAVVEFVGYGGGRGLPQDRLTWASAGYAHLVV 115 Query: 120 DVRGQLGETGN----LLPMESGVAK-GWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQG 174 D RGQ G+ P+ V+ G++T+G+L+K+ YY V D VRAV A L Sbjct: 116 DTRGQGAAWGSGGATADPVGYDVSTPGFMTRGILDKDTYYYRRVFTDAVRAVRAARSLPE 175 Query: 175 VDPSRXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYV 233 V V AD+P L + + + TE+ Y+ Sbjct: 176 VRADAVCVAGASQGGAIALAAASLSPDVRAVMADVPFLSWFRRALQITAVEPYTEVVRYL 235 Query: 234 RRYPEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIM 293 + +H+ERVLDTL +FD+ +L PR TAP LIS+ D VC P +V+AAY R ++ Sbjct: 236 SVHRDHVERVLDTLDHFDVAHLVPRATAPALISLALMDRVCPPSTVWAAYRRYGGPAELS 295 Query: 294 DYPFSGHEVSEYHLRQKVL 312 +PF+ HE + H ++ L Sbjct: 296 VHPFNDHEGGQTHQWRRQL 314 >B1DK58.1 B1DK58_9BACL Acetyl xylan esterase Length = 321 Score = 158 bits (400), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 93/305 (30%), Positives = 145/305 (47%), Gaps = 11/305 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVT----PYPGMR 56 M +E ++L P P + ++ FW + + PLE PE T ++ Sbjct: 1 MGYVEEVTKDLYQYLP-PLTGKDDLDSFWSATIEQAKSVPLE----PERTEINYSSKHVK 55 Query: 57 VDKVSFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGDRGYPERYASWLLLGYSV 116 V ++F DETR+ W I PA PC+I + G++G RG P + W+++G +V Sbjct: 56 VYDIAFNGLDETRIRGWLIVPAFLKQE--KYPCLIHYHGFSGSRGEPAGHMHWVMMGMAV 113 Query: 117 LAVDVRGQLGETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVD 176 LAVD R Q G TGN G ++G+ K YY MD ++A++ A +D Sbjct: 114 LAVDCRDQGGPTGNSASYTHGFLSNVASKGVHNKWEYYYRYAYMDCLKAIDFACAQSDID 173 Query: 177 PSRXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGVLNSTSSLTEIGAYVRRY 236 + D+P+ L + + + + Y++R+ Sbjct: 174 ADKIIIEGGSQGGALGMAVVALDNRPKLAMVDVPSNSNLVARIEGNHGAFGAVAEYLKRH 233 Query: 237 PEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDYP 296 PE + V+D L+YFD +NLA RI PVL SV KD C + +A YNRI+++K+I+ YP Sbjct: 234 PEQTDLVMDNLSYFDTMNLAHRIACPVLASVALKDETCPAQMYFATYNRIQTEKEIVIYP 293 Query: 297 FSGHE 301 F+GHE Sbjct: 294 FNGHE 298 >B4AIZ3.1 B4AIZ3_BACPU Cephalosporin-C deacetylase Length = 318 Score = 157 bits (398), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 104/323 (32%), Positives = 154/323 (47%), Gaps = 12/323 (3%) Query: 1 MNTIELRKQELENCRPEPTLEQGVVNEFWDGILAEYARKPLEIISVPEVTPYPGMRVDKV 60 M +L EL+ +P+ T ++FW L E + E P P G++V ++ Sbjct: 1 MQLFDLPLDELKKYKPKKTARPDF-SDFWKASLEELNQVEAEPALEPYDYPVKGVKVYRL 59 Query: 61 SFQSFDETRVHAWHIQPAQAGNGGGPLPCIITFPGYTGD-RGYPERYASWLLLGYSVLAV 119 +FQSF ++++ W+ P Q G P P ++ F GY G +W L GY+ + Sbjct: 60 TFQSFGQSQIEGWYAVPDQTG----PHPALVRFHGYNASYDGDIHDIVNWALHGYATFGM 115 Query: 120 DVRGQLG-ETGNLLPMESGVAKGWITQGLLEKEHSYYMAVAMDTVRAVETAAQLQGVDPS 178 VRGQ G E + P A GW+T+G+L KE YY V +D VRA++ VD Sbjct: 116 LVRGQGGSEDKTVTP--GAQALGWMTKGILSKETYYYRGVYLDAVRALDVIQSFPEVDEH 173 Query: 179 RXXXXXXXXXXXXXXXXXXXXXXXXXVAADIPNLCRLDFGV-LNSTSSLTEIGAYVRRY- 236 R V AD P L + V + EI +Y RR Sbjct: 174 RIGVVGGSQGGALAVAAAALSDIPKVVVADYPYLSNFERAVDMALEHPYLEINSYFRRNS 233 Query: 237 -PEHLERVLDTLAYFDIVNLAPRITAPVLISVGWKDTVCMPESVYAAYNRIESDKQIMDY 295 PE E+V +TL+YFD++NLA + P L+++G D + P +V+A YN +E+DK + Y Sbjct: 234 DPEAEEKVFETLSYFDLINLAGWVKQPTLMAIGLIDQITPPSTVFAVYNHLETDKDLKVY 293 Query: 296 PFSGHEVSEYHLRQKVLFLQKHL 318 + GHE +K+ FLQKHL Sbjct: 294 RYFGHEYIPAFQTEKLAFLQKHL 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,911,081,768 Number of extensions: 81055133 Number of successful extensions: 186083 Number of sequences better than 1.0e-05: 164 Number of HSP's gapped: 185840 Number of HSP's successfully gapped: 175 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)