BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0026.p (281 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1ZRW7.1 B1ZRW7_OPITP Xylose isomerase domain protein TIM barrel 197 1e-48 B1DCC5.1 B1DCC5_9BACL Xylose isomerase domain protein TIM barrel 183 2e-44 A6LQK8.1 A6LQK8_CLOB8 Xylose isomerase domain protein TIM barrel 183 2e-44 Q2B7P4.1 Q2B7P4_9BACI Putative uncharacterized protein 182 2e-44 C4C732.1 C4C732_9FUSO Sugar phosphate isomerase/epimerase 181 5e-44 >B1ZRW7.1 B1ZRW7_OPITP Xylose isomerase domain protein TIM barrel Length = 278 Score = 197 bits (500), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 110/277 (39%), Positives = 158/277 (57%), Gaps = 4/277 (1%) Query: 4 IRYGTLAHTAGCLPLKELTAALQDYGIDFVQLALSKAIQDIDTSTGKLSPGLANYIAEQF 63 + G AH G LP EL A + +G+ VQLA KAI D ST +L+P A + F Sbjct: 1 MNLGVRAHDFGQLPPDELAATIARHGLSCVQLAPPKAIAGCDGSTARLTPEFARRVHTAF 60 Query: 64 DRAGIRIGVLGCYINPIHPDPAIRRLEIGRFKEHLRYARQFGAPMVATETGALTTFAEFE 123 R G+ I VLGCYIN + + RR ++ RFK +LR AR FG P+V TETG+L + Sbjct: 61 QRHGVEISVLGCYINLADRNESHRRAQLERFKAYLRLARDFGGPIVGTETGSLNSDFSRH 120 Query: 124 PYRYEELGWETLRATVEELAEEAEKWGVFLGLEGVSTHTLSTPAKVRRILDEVPSSAIGV 183 P + ++ + A+V ELA+EAEK G +G+E V + +S+P +++R+L+ V S ++ V Sbjct: 121 PENAGDEAFDLVVASVRELADEAEKCGAIVGIEAVERYVISSPPRLKRLLEAVDSRSVQV 180 Query: 184 VFDPCNLI-GDNVQNQDEIVDDAFSLFGDRIILAHLKDMYAEGIRIRHGQAGRGLFHTAE 242 ++DP NL+ N Q QDEI+D+A L GDRI + H KD + + AGRG H Sbjct: 181 IYDPVNLLWSTNHQRQDEIMDEAHQLLGDRIRIVHAKDFTVDAGKFHEHPAGRGALHQER 240 Query: 243 FLNKLQACKPMIDVSLEDIQGPAIRETVALLERLRDT 279 ++ L+A + V LE+ I TVA +RDT Sbjct: 241 WMRWLKAQPRDVPVLLENTHPSTIAHTVAF---MRDT 274 >B1DCC5.1 B1DCC5_9BACL Xylose isomerase domain protein TIM barrel Length = 276 Score = 183 bits (464), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 115/259 (44%), Positives = 148/259 (57%), Gaps = 10/259 (3%) Query: 7 GTLAHTAGCLPLKELTAALQDYGIDFVQLALSKAIQDIDTSTGKLSPGLANYIAEQFDRA 66 G LAH G LP KEL A + G +VQLAL KA D+D+ GKLSPGLA + F Sbjct: 4 GILAHLLGTLPYKELAAKISANGFPYVQLALGKAFSDVDSRLGKLSPGLAQEVGGAFADN 63 Query: 67 GIRIGVLGCYINPIHPDPAIRRLEIGRFKEHLRYARQFGAPMVATETGALTTFAEFEPYR 126 G+RI VLGCY + + D + R + RFKEHLR AR FGAP+VATE G +T +P R Sbjct: 64 GVRIAVLGCYASLVDLDDEVYRHNVDRFKEHLRNARHFGAPIVATEVGGIT-----DPER 118 Query: 127 YEELGWETLRATVEELAEEAEKWGVFLGLEGVSTHTLSTPAKVRRILDEVPSSAIGVVFD 186 E E L +EEL EEAE+WGV +GLE H + T + ++ PSS +GVV D Sbjct: 119 RAE-HIERLNRALEELVEEAERWGVTIGLEAAQGHMIDTAETLAETIERFPSSCVGVVMD 177 Query: 187 PCNLIGD-NVQNQDEIVDDAFSLFGDRIILAHLKDMY--AEGIRIRHGQAGRGLFHTAEF 243 PCNL+ +NQD V +AF L G RI+ AH+KD+ A+G I AG G + F Sbjct: 178 PCNLMNPARFENQDHTVREAFRLLGPRIVSAHVKDLNRAADGALIETA-AGLGDLNYPLF 236 Query: 244 LNKLQACKPMIDVSLEDIQ 262 L+ KP ++E + Sbjct: 237 FELLEQHKPYGFATMEAVS 255 >A6LQK8.1 A6LQK8_CLOB8 Xylose isomerase domain protein TIM barrel Length = 278 Score = 183 bits (464), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 93/275 (33%), Positives = 154/275 (56%), Gaps = 3/275 (1%) Query: 4 IRYGTLAHTAGCLPLKELTAALQDYGIDFVQLALSKAIQDIDTSTGKLSPGLANYIAEQF 63 +R G H G L ++ +++ G+ VQ K I + + + G ++PG+A YI Sbjct: 1 MRIGVRGHDIGLYKLDDIGRIMKEKGLKSVQFVAKKIISEFEVTRGCMTPGMAQYINNTL 60 Query: 64 DRAGIRIGVLGCYINPIHPDPAIRRLEIGRFKEHLRYARQFGAPMVATETGALTT-FAEF 122 ++ + I +LGCYIN +PD + + FKEH+R+A+ G +V TETGA + Sbjct: 61 NKYEVNISILGCYINLANPDDNELKESLDTFKEHIRFAKYLGCNVVGTETGAFNKEYIYT 120 Query: 123 EPYRYEELGWETLRATVEELAEEAEKWGVFLGLEGVSTHTLSTPAKVRRILDEVPSSAIG 182 E EE +L + + L +EAEK+G+ +G+EGV+ H ++TP +++R LD V S+ + Sbjct: 121 EKNNTEEAFLRSLNS-IRILVKEAEKFGIVVGIEGVTKHIMNTPERLKRALDNVESNNLQ 179 Query: 183 VVFDPCNLIGD-NVQNQDEIVDDAFSLFGDRIILAHLKDMYAEGIRIRHGQAGRGLFHTA 241 V+FDP N I + N QDEI+ +F LFGDRI++ H KD + +++ G+G F+ Sbjct: 180 VIFDPINFIDESNYDKQDEIIRKSFELFGDRIVIIHAKDFIYKDGKVQQVSIGKGQFNYP 239 Query: 242 EFLNKLQACKPMIDVSLEDIQGPAIRETVALLERL 276 L ++ KP ID+ LED + ++ +E + Sbjct: 240 LLLKLIKERKPYIDIILEDTIPEDLDSSIKYIEEI 274 >Q2B7P4.1 Q2B7P4_9BACI Putative uncharacterized protein Length = 286 Score = 182 bits (463), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 102/264 (38%), Positives = 154/264 (58%), Gaps = 1/264 (0%) Query: 4 IRYGTLAHTAGCLPLKELTAALQDYGIDFVQLALSKAIQDIDTSTGKLSPGLANYIAEQF 63 + G AH L+EL + G +QLAL K++ + S G LSPGL+ YI + F Sbjct: 1 MNLGIRAHDIEKDSLEELAEEIGRKGFSCIQLALGKSLPALYPSPGTLSPGLSRYIGKTF 60 Query: 64 DRAGIRIGVLGCYINPIHPDPAIRRLEIGRFKEHLRYARQFGAPMVATETGALTTFAEFE 123 AG++I VLGCY+N IHPD + RR + +FKEH+RYAR FG +V TETG++ + Sbjct: 61 GDAGVQIAVLGCYVNLIHPDLSERRKGLEKFKEHIRYARDFGCSIVGTETGSVLPEMGYS 120 Query: 124 PYRYEELGWETLRATVEELAEEAEKWGVFLGLEGVSTHTLSTPAKVRRILDEVPSSAIGV 183 +EE + +V ELA EAE++GV +G+E H + + +++R+LD V S+ + V Sbjct: 121 ERNFEEKPFLDAVESVAELALEAERFGVIVGVEAGVNHPVHSADRLKRLLDLVDSNNVQV 180 Query: 184 VFDPCNLIG-DNVQNQDEIVDDAFSLFGDRIILAHLKDMYAEGIRIRHGQAGRGLFHTAE 242 +FDP NLI +N QDE++ +A L G++I + H KD E +++ AG+GL + + Sbjct: 181 IFDPANLITVENYHRQDELLAEAIELLGEKIAIFHAKDFVVEEDQVKIVPAGQGLLNYSL 240 Query: 243 FLNKLQACKPMIDVSLEDIQGPAI 266 L+ KP I V +E + P I Sbjct: 241 ISKLLKDRKPYIQVLMEGTKEPDI 264 >C4C732.1 C4C732_9FUSO Sugar phosphate isomerase/epimerase Length = 278 Score = 181 bits (460), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 94/268 (35%), Positives = 149/268 (55%), Gaps = 1/268 (0%) Query: 7 GTLAHTAGCLPLKELTAALQDYGIDFVQLALSKAIQDIDTSTGKLSPGLANYIAEQFDRA 66 G AH + +EL + G VQLAL K+ + I++ G LSPG NYI Sbjct: 4 GIRAHDFTAVSAEELAEKISAKGFKNVQLALPKSFETINSDLGNLSPGFGNYIRNALSEK 63 Query: 67 GIRIGVLGCYINPIHPDPAIRRLEIGRFKEHLRYARQFGAPMVATETGALTTFAEFEPYR 126 I + VL CY N + PD RR I +FKE++RYA FGA +V +ETG+ + Sbjct: 64 NIHVAVLSCYFNMVDPDEEFRRHCIDKFKEYIRYASTFGAKIVGSETGSYNRDFSYHEDN 123 Query: 127 YEELGWETLRATVEELAEEAEKWGVFLGLEGVSTHTLSTPAKVRRILDEVPSSAIGVVFD 186 + E ++T++ T+ ELA+EAEK+G+ +G+E T T+ TP +++R+LDE+ S+ + V++D Sbjct: 124 HSEKAFQTVKRTIRELADEAEKFGIIIGVENSVTLTIHTPERMKRLLDEIGSNNVQVLYD 183 Query: 187 PCNLI-GDNVQNQDEIVDDAFSLFGDRIILAHLKDMYAEGIRIRHGQAGRGLFHTAEFLN 245 P I D + NQDEI+ + + GDRI H+KD E G+G+ + Sbjct: 184 PIGFIRADEIMNQDEIITRSLDILGDRIAAVHVKDFIVENGERVIVPPGKGILNFELLFK 243 Query: 246 KLQACKPMIDVSLEDIQGPAIRETVALL 273 L+ KP I++ LE+I+ + +++ + Sbjct: 244 LLKKKKPFIEIILENIEVNEMDKSINYM 271 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,665,564,535 Number of extensions: 69633432 Number of successful extensions: 201442 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 201432 Number of HSP's successfully gapped: 64 Length of query: 281 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 147 Effective length of database: 1,798,280,193 Effective search space: 264347188371 Effective search space used: 264347188371 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)