BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0022.p (257 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B0K805.1 B0K805_THEP3 Lipolytic enzyme, G-D-S-L family 299 1e-79 B0K0Z6.1 B0K0Z6_THEPX Lipolytic enzyme, G-D-S-L family 299 1e-79 B7R6R2.1 B7R6R2_9THEO GDSL-like lipase/acylhydrolase, putative 285 2e-75 Q8RDJ0.1 Q8RDJ0_THETN Putative uncharacterized protein 285 3e-75 B8D9Z9.1 B8D9Z9_LISMH Lipase/acylhydrolase, putative 191 4e-47 >B0K805.1 B0K805_THEP3 Lipolytic enzyme, G-D-S-L family Length = 241 Score = 299 bits (766), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 137/230 (59%), Positives = 178/230 (77%) Query: 17 YKYMVSGDSISKGVVYDEARSKYVILEDNYVSLLQGKLKGALRNTARFGNTLLKGFGNLK 76 Y +V+GDSISKGVVY + +SKYVI + NYV+LL+ +KG ++N A+FGNTLLKG LK Sbjct: 10 YDIVVAGDSISKGVVYSKEKSKYVISDVNYVNLLKDNIKGTVKNIAKFGNTLLKGIDILK 69 Query: 77 RDVLKEKPDMVLIEYGGNDCDFHWEEIVNNPEAEHNPKTDFPAFESMLRDMIDFLSTQGI 136 R +LK+KPD+VLIE+GGNDCDF WEEI NP EH+PKTDF F+ L+++I L I Sbjct: 70 RYLLKDKPDIVLIEFGGNDCDFDWEEIAKNPYDEHSPKTDFYIFKEKLKELIKSLEDANI 129 Query: 137 MPILMSLPPLNADNYFKWVSGNNPASEANIMKFLGSVTKIYWWQERYNSTIIRVAESTKT 196 +P+LM+LPPL+AD YFKW+S N+ NI+K+LGSVTKIYWWQE+YNS I+ +AE TKT Sbjct: 130 IPVLMTLPPLDADRYFKWISKNSEEMGKNILKWLGSVTKIYWWQEKYNSAILSIAEETKT 189 Query: 197 KIIDVRSAFLQQPDYTKFICRDGIHPNRAGHRIIYDKVLEFLSSSEPHLL 246 ++ID+RSAFL PD+ +FIC DGIHPN GH+II K+ E+L + +LL Sbjct: 190 RLIDIRSAFLDYPDFRQFICTDGIHPNEKGHKIIAHKIYEYLQRNYAYLL 239 >B0K0Z6.1 B0K0Z6_THEPX Lipolytic enzyme, G-D-S-L family Length = 241 Score = 299 bits (766), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 137/230 (59%), Positives = 178/230 (77%) Query: 17 YKYMVSGDSISKGVVYDEARSKYVILEDNYVSLLQGKLKGALRNTARFGNTLLKGFGNLK 76 Y +V+GDSISKGVVY + +SKYVI + NYV+LL+ +KG ++N A+FGNTLLKG LK Sbjct: 10 YDIVVAGDSISKGVVYSKEKSKYVISDVNYVNLLKDNIKGTVKNIAKFGNTLLKGIDILK 69 Query: 77 RDVLKEKPDMVLIEYGGNDCDFHWEEIVNNPEAEHNPKTDFPAFESMLRDMIDFLSTQGI 136 R +LK+KPD+VLIE+GGNDCDF WEEI NP EH+PKTDF F+ L+++I L I Sbjct: 70 RYLLKDKPDIVLIEFGGNDCDFDWEEIAKNPYDEHSPKTDFYIFKEKLKELIKSLEDANI 129 Query: 137 MPILMSLPPLNADNYFKWVSGNNPASEANIMKFLGSVTKIYWWQERYNSTIIRVAESTKT 196 +P+LM+LPPL+AD YFKW+S N+ NI+K+LGSVTKIYWWQE+YNS I+ +AE TKT Sbjct: 130 IPVLMTLPPLDADRYFKWISKNSEEMGKNILKWLGSVTKIYWWQEKYNSAILSIAEETKT 189 Query: 197 KIIDVRSAFLQQPDYTKFICRDGIHPNRAGHRIIYDKVLEFLSSSEPHLL 246 ++ID+RSAFL PD+ +FIC DGIHPN GH+II K+ E+L + +LL Sbjct: 190 RLIDIRSAFLDYPDFRQFICTDGIHPNEKGHKIIAHKIYEYLQRNYAYLL 239 >B7R6R2.1 B7R6R2_9THEO GDSL-like lipase/acylhydrolase, putative Length = 237 Score = 285 bits (730), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 125/230 (54%), Positives = 174/230 (75%) Query: 17 YKYMVSGDSISKGVVYDEARSKYVILEDNYVSLLQGKLKGALRNTARFGNTLLKGFGNLK 76 Y +++GDSIS+GVV D ++KYVI E NYV+L++G++KG ++N +RFG T+LKG +K Sbjct: 6 YSILLAGDSISRGVVLDREKNKYVITERNYVNLVKGRIKGTIKNISRFGATILKGIEMIK 65 Query: 77 RDVLKEKPDMVLIEYGGNDCDFHWEEIVNNPEAEHNPKTDFPAFESMLRDMIDFLSTQGI 136 ++ ++EKPD+V++E+GGNDCDF WE++ NPE EH PKTD+ F L+++I+FL + Sbjct: 66 KEFVREKPDVVVLEFGGNDCDFDWEQVAKNPEMEHFPKTDYQLFIQKLKEIIEFLKEFDV 125 Query: 137 MPILMSLPPLNADNYFKWVSGNNPASEANIMKFLGSVTKIYWWQERYNSTIIRVAESTKT 196 +P+LM+LPPL+AD Y KW+S + E NI+++LGSVTKIYWWQERYNS I +AE TKT Sbjct: 126 VPVLMTLPPLDADRYLKWISKASKEMEENILRWLGSVTKIYWWQERYNSAIRIIAEETKT 185 Query: 197 KIIDVRSAFLQQPDYTKFICRDGIHPNRAGHRIIYDKVLEFLSSSEPHLL 246 +IDVRSAFL PD+ F+C DGIHPN GH+I+ K+ E+L P LL Sbjct: 186 LVIDVRSAFLYYPDFRNFLCEDGIHPNEEGHKIMAHKIGEYLKKHYPFLL 235 >Q8RDJ0.1 Q8RDJ0_THETN Putative uncharacterized protein Length = 237 Score = 285 bits (729), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 125/230 (54%), Positives = 174/230 (75%) Query: 17 YKYMVSGDSISKGVVYDEARSKYVILEDNYVSLLQGKLKGALRNTARFGNTLLKGFGNLK 76 Y +++GDSIS+GVV D ++KYVI E NYV+L++G++KG ++N +RFG T+LKG +K Sbjct: 6 YSILLAGDSISRGVVLDREKNKYVITERNYVNLVKGRIKGTIKNISRFGATILKGIEMIK 65 Query: 77 RDVLKEKPDMVLIEYGGNDCDFHWEEIVNNPEAEHNPKTDFPAFESMLRDMIDFLSTQGI 136 ++ ++EKPD+V++E+GGNDCDF WE++ NPE EH PKTD+ F L+++I+FL + Sbjct: 66 KEFVREKPDVVVLEFGGNDCDFDWEQVAKNPEMEHFPKTDYHLFIQKLKEIIEFLKEFEV 125 Query: 137 MPILMSLPPLNADNYFKWVSGNNPASEANIMKFLGSVTKIYWWQERYNSTIIRVAESTKT 196 +P+LM+LPPL+AD Y KW+S + E NI+++LGSVTKIYWWQERYNS I +AE TKT Sbjct: 126 VPVLMTLPPLDADRYLKWISKASKEVEENILRWLGSVTKIYWWQERYNSAIRVIAEETKT 185 Query: 197 KIIDVRSAFLQQPDYTKFICRDGIHPNRAGHRIIYDKVLEFLSSSEPHLL 246 +IDVRSAFL PD+ F+C DGIHPN GH+I+ K+ E+L P LL Sbjct: 186 LVIDVRSAFLYYPDFRNFLCEDGIHPNEEGHKIMAHKIGEYLKKHYPFLL 235 >B8D9Z9.1 B8D9Z9_LISMH Lipase/acylhydrolase, putative Length = 233 Score = 191 bits (486), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 97/221 (43%), Positives = 141/221 (63%), Gaps = 3/221 (1%) Query: 21 VSGDSISKGVVYDEARSKYVILEDNYVSLLQGKLKGALRNTARFGNTLLKGFGNLKRDVL 80 V GDSI KGV+++ +++Y +L++N + KL +N + G T+ KG L +D+ Sbjct: 10 VWGDSIMKGVLFNPEKNRYTLLKNNCIKRASEKLGLTFQNHSTMGRTITKGHEILTKDLA 69 Query: 81 KEKP-DMVLIEYGGNDCDFHWEEIVNNPEAEHNPKTDFPAFESMLRDMIDFLSTQGIMPI 139 K+ D+ +IE+GGNDCDF+W E+ NP AEH P T FE+ L +MI L I PI Sbjct: 70 KDATNDIAIIEFGGNDCDFNWTEVSENPTAEHIPSTPLNIFETQLLEMIARLKKLDIKPI 129 Query: 140 LMSLPPLNADNYFKWVSGNNPASEANIMKFLGS-VTKIYWWQERYNSTIIRVAESTKTKI 198 LM+LPPL+A YF++++ N ++ NI++FLG V IY WQERY++TI R+A T + I Sbjct: 130 LMTLPPLHAKRYFEFIT-RNGLNKDNILQFLGGDVEMIYRWQERYSNTISRIAAETGSHI 188 Query: 199 IDVRSAFLQQPDYTKFICRDGIHPNRAGHRIIYDKVLEFLS 239 IDVR +FL + Y +C DGIHPN AGH + K +++ S Sbjct: 189 IDVRDSFLAEFHYENLLCADGIHPNEAGHIAMSQKFIQYAS 229 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,576,637,047 Number of extensions: 64670693 Number of successful extensions: 145280 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 145288 Number of HSP's successfully gapped: 20 Length of query: 257 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 124 Effective length of database: 1,807,701,208 Effective search space: 224154949792 Effective search space used: 224154949792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)