BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0017.p (270 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q92DZ4.1 Q92DZ4_LISIN ThiD protein 357 7e-97 Q722N3.1 Q722N3_LISMF Phosphomethylpyrimidine kinase EC=2.7.4.7 356 1e-96 Q4ELL5.1 Q4ELL5_LISMO Phosphomethylpyrimidine kinase EC=2.7.4.7 356 1e-96 A0AGB6.1 A0AGB6_LISW6 ThiD protein EC=2.7.4.7 356 1e-96 B8DIC4.1 B8DIC4_LISMH Phosphomethylpyrimidine kinase EC=2.7.4.7 356 1e-96 >Q92DZ4.1 Q92DZ4_LISIN ThiD protein Length = 271 Score = 357 bits (915), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 168/243 (69%), Positives = 201/243 (82%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTFEEYGTYGFSA+TTIVTMDPDN W H V PIDA +V EQLKT+ +GGPVDAMKTGML Sbjct: 24 LKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTGML 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 GS+DI+K +AI + NVVIDPVMVCKGEDE++ PE+A AIRDLLLP AT+ TPNLF Sbjct: 84 GSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLF 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKLT++D+MK+AA+ I LG + VV+KGGKAL D AID+ +DG E+TV E Sbjct: 144 EAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTVYEV 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 KI P++NHGAGCTFAAAIT GLA GL+V +AV KAKDFV+AAI+ G+A N+++GPV+HG Sbjct: 204 EKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKDFVTAAIKGGFALNEFIGPVWHG 263 Query: 265 GYR 267 Y Sbjct: 264 AYN 266 >Q722N3.1 Q722N3_LISMF Phosphomethylpyrimidine kinase EC=2.7.4.7 Length = 271 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 167/243 (68%), Positives = 201/243 (82%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTFEEYGTYGFSA+TTIVTMDPDN W H V PIDA +V EQLKT+ +GGPVDAMKTGML Sbjct: 24 LKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTGML 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 GS+DI+K +AI + NVVIDPVMVCKGEDE++ PE+A AIRDLLLP AT+ TPNLF Sbjct: 84 GSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLF 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKLT++D+MK+AA+ I LG + VV+KGGKAL D AID+ +DG E+T+ E Sbjct: 144 EAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTIYEV 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 KI P++NHGAGCTFAAAIT GLA GL+V +AV KAKDFV+AAI+ G+A N+++GPV+HG Sbjct: 204 EKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKDFVTAAIKGGFALNEFIGPVWHG 263 Query: 265 GYR 267 Y Sbjct: 264 AYN 266 >Q4ELL5.1 Q4ELL5_LISMO Phosphomethylpyrimidine kinase EC=2.7.4.7 Length = 271 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 167/243 (68%), Positives = 201/243 (82%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTFEEYGTYGFSA+TTIVTMDPDN W H V PIDA +V EQLKT+ +GGPVDAMKTGML Sbjct: 24 LKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTGML 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 GS+DI+K +AI + NVVIDPVMVCKGEDE++ PE+A AIRDLLLP AT+ TPNLF Sbjct: 84 GSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLF 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKLT++D+MK+AA+ I LG + VV+KGGKAL D AID+ +DG E+T+ E Sbjct: 144 EAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTIYEV 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 KI P++NHGAGCTFAAAIT GLA GL+V +AV KAKDFV+AAI+ G+A N+++GPV+HG Sbjct: 204 EKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKDFVTAAIKGGFALNEFIGPVWHG 263 Query: 265 GYR 267 Y Sbjct: 264 AYN 266 >A0AGB6.1 A0AGB6_LISW6 ThiD protein EC=2.7.4.7 Length = 271 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 168/243 (69%), Positives = 201/243 (82%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTFEEYGTYGFSA+TTIVTMDPDN W H V PIDA +V EQLKT+ +GGPVDAMKTGML Sbjct: 24 LKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTGML 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 GS+DI+K +AI + NVVIDPVMVCKGEDE++ PE+A AIRDLLLP AT+ TPNLF Sbjct: 84 GSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLF 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKLT++D+MK+AA+ I LG + VV+KGGKAL D AID+ +DG E+TV E Sbjct: 144 EAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGKEFTVYEV 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 KI P++NHGAGCTFAAAIT GLA GL+V +AV KAKDFV+AAI+ G+A N+++GPV+HG Sbjct: 204 EKIAPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKDFVTAAIKGGFALNEFIGPVWHG 263 Query: 265 GYR 267 Y Sbjct: 264 AYN 266 >B8DIC4.1 B8DIC4_LISMH Phosphomethylpyrimidine kinase EC=2.7.4.7 Length = 271 Score = 356 bits (914), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 167/243 (68%), Positives = 201/243 (82%) Query: 25 LKTFEEYGTYGFSALTTIVTMDPDNGWHHNVYPIDAAIVAEQLKTVFAGGPVDAMKTGML 84 LKTFEEYGTYGFSA+TTIVTMDPDN W H V PIDA +V EQLKT+ +GGPVDAMKTGML Sbjct: 24 LKTFEEYGTYGFSAITTIVTMDPDNNWAHGVTPIDAQLVREQLKTILSGGPVDAMKTGML 83 Query: 85 GSVDIVKVAEKAIKENLQTNVVIDPVMVCKGEDEVLNPESANAIRDLLLPLATVATPNLF 144 GS+DI+K +AI + NVVIDPVMVCKGEDE++ PE+A AIRDLLLP AT+ TPNLF Sbjct: 84 GSIDIIKATREAIDKYDLKNVVIDPVMVCKGEDELIQPENAEAIRDLLLPKATITTPNLF 143 Query: 145 EAGVLSGLGKLTSIDEMKEAARLIHALGTQNVVVKGGKALGGDLAIDVFFDGSEYTVLET 204 EAG LSGLGKLT++D+MK+AA+ I LG + VV+KGGKAL D AID+ +DG E+T+ E Sbjct: 144 EAGQLSGLGKLTTLDDMKKAAKKIIELGAKYVVIKGGKALESDKAIDLLYDGEEFTIYEV 203 Query: 205 AKIEPAYNHGAGCTFAAAITGGLANGLSVHDAVVKAKDFVSAAIRSGYAFNQYVGPVFHG 264 KI P++NHGAGCTFAAAIT GLA GL+V +AV KAKDFV+AAI+ G+A N+++GPV+HG Sbjct: 204 EKISPSHNHGAGCTFAAAITAGLAKGLTVEEAVAKAKDFVTAAIKGGFALNEFIGPVWHG 263 Query: 265 GYR 267 Y Sbjct: 264 AYN 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,481,904,654 Number of extensions: 59109946 Number of successful extensions: 176592 Number of sequences better than 1.0e-05: 1842 Number of HSP's gapped: 173747 Number of HSP's successfully gapped: 1843 Length of query: 270 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 137 Effective length of database: 1,807,701,208 Effective search space: 247655065496 Effective search space used: 247655065496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)