BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0015.p (314 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q13FA2.1 Q13FA2_BURXL Putative oxygenase 370 e-100 Q399G0.1 Q399G0_BURS3 Rieske (2Fe-2S) protein EC=1.14.13.82 357 7e-97 B4WN54.1 B4WN54_9SYNE Rieske (2Fe-2S) domain protein 354 5e-96 Q0BUD6.1 Q0BUD6_GRABC Vanillate demethylase oxygenase subunit EC... 342 4e-92 B0VQN7.1 B0VQN7_ACIBS Putative vanillate O-demethylase oxygenase... 341 6e-92 >Q13FA2.1 Q13FA2_BURXL Putative oxygenase Length = 359 Score = 370 bits (950), Expect = e-100, Method: Compositional matrix adjust. Identities = 179/314 (57%), Positives = 221/314 (70%), Gaps = 2/314 (0%) Query: 1 VAIADEVQDKPLAVKLLDMKLVCYRSEGKVVIARDLCFHRGAPLSKGWVENGDIVCPYHG 60 VA + +V+ P+AV LLD++LV YR+ V +ARDLC HRG PLS G VE ++VC YHG Sbjct: 44 VARSVDVKSSPVAVTLLDLELVTYRTPAGVHVARDLCPHRGVPLSMGCVEGDELVCAYHG 103 Query: 61 FRYNCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLG--SAAEQIPDFPGWEDP 118 RY +G+C +PA P K S K +L V+PAVERYGL+WTCL +P P W DP Sbjct: 104 LRYAADGQCRKIPAQPELKPSAKFRLTVFPAVERYGLVWTCLNPQGGEANLPPMPSWGDP 163 Query: 119 DYINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNELVAE 178 +ILPP DIAGS+GRQ+EGF+DV+HFA+VH F DRN EVP Y+ L E Sbjct: 164 LCQSILPPFVDIAGSAGRQVEGFVDVAHFAFVHHDAFADRNRPEVPMYQTEFTDFGLRTE 223 Query: 179 YWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLNILNCASPVSARYT 238 YWS VSNY K ++ AP+ F WLR + ++PPF A LTV+FP+EGRLNILN ASPVSAR T Sbjct: 224 YWSNVSNYPKALQHLAPDDFSWLRVYEIYPPFCARLTVHFPNEGRLNILNAASPVSARKT 283 Query: 239 RLFCPITRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHIPADRTSIA 298 RLF PITRNFD + ++D FN Q+FAED +VE Q PEDLPLD+QAEAH ADRTS+ Sbjct: 284 RLFVPITRNFDTTGSLDDVYAFNAQIFAEDQAIVERQRPEDLPLDMQAEAHFAADRTSVG 343 Query: 299 YRQLLTELGLGRSY 312 YR+LL E+GL +Y Sbjct: 344 YRRLLREMGLTMAY 357 >Q399G0.1 Q399G0_BURS3 Rieske (2Fe-2S) protein EC=1.14.13.82 Length = 351 Score = 357 bits (917), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 175/313 (55%), Positives = 213/313 (68%) Query: 1 VAIADEVQDKPLAVKLLDMKLVCYRSEGKVVIARDLCFHRGAPLSKGWVENGDIVCPYHG 60 VA A EV D+PLAV LLD ++V +RS G +V ARD+C HRGAPLS+GWV +VCPYHG Sbjct: 38 VAFATEVGDRPLAVTLLDERIVLFRSGGAIVAARDVCPHRGAPLSRGWVVEDRLVCPYHG 97 Query: 61 FRYNCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLGSAAEQIPDFPGWEDPDY 120 Y +GKC +P+ P I +L L Y A + YGL+W LG +PDFP W + Sbjct: 98 LEYAADGKCVRIPSQPDGPIPERLCLTTYAAQQAYGLVWVSLGGGELPLPDFPAWNAEGF 157 Query: 121 INILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNELVAEYW 180 ILPP DI S+GRQ EGF+DV+HFA++H +F DR N VPQY V R N L AEY Sbjct: 158 QQILPPSIDIRASAGRQTEGFIDVAHFAWIHHESFADRRNPVVPQYSVERRENGLHAEYV 217 Query: 181 STVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLNILNCASPVSARYTRL 240 STVSN+ K ++ APEGF+W R F V PF A LTV+FP+ GRL ILN ASPVSAR TRL Sbjct: 218 STVSNFPKSMQHRAPEGFLWRRVFEVDVPFFARLTVHFPEGGRLAILNAASPVSARLTRL 277 Query: 241 FCPITRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHIPADRTSIAYR 300 F PI RNFD P++D +FN QVF ED ++VE Q PEDLP+D EA I ADR+S AYR Sbjct: 278 FVPIARNFDHDLPLDDVYEFNRQVFEEDREIVEQQCPEDLPIDRSHEAPILADRSSGAYR 337 Query: 301 QLLTELGLGRSYT 313 + L E+GLG+SY Sbjct: 338 RALGEIGLGQSYA 350 >B4WN54.1 B4WN54_9SYNE Rieske (2Fe-2S) domain protein Length = 363 Score = 354 bits (909), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 171/319 (53%), Positives = 220/319 (68%), Gaps = 5/319 (1%) Query: 1 VAIADEVQDKPLAVKLLDMKLVCYR----SEGKVVIARDLCFHRGAPLSKGWVENGDIVC 56 +A + +V +P+ LLD +LV +R S ++ +A D+C HRG PLS G +EN ++C Sbjct: 45 IAFSKDVTTEPVGTTLLDQRLVIWRPPASSGSQLCVANDICIHRGVPLSMGHIENEQLIC 104 Query: 57 PYHGFRYNCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCL-GSAAEQIPDFPGW 115 YHGF Y+ G+CT +PA+P +KI +L L YP E YGL+WT L G +P FP W Sbjct: 105 KYHGFHYSASGQCTKIPANPEAKIPARLCLKTYPVKEAYGLVWTTLSGDENTPLPAFPEW 164 Query: 116 EDPDYINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNEL 175 E+ DY ILP D++ ++GRQMEGFLDV+HFA+VH+ +FGDRNN VP Y V + L Sbjct: 165 ENDDYQPILPNSVDVSAAAGRQMEGFLDVAHFAWVHSESFGDRNNPVVPSYSVTPTAHGL 224 Query: 176 VAEYWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLNILNCASPVSA 235 A+Y STVSN+ KG ++ AP+ F WLR F VF PF A L V+FP+EGRL ILN ASP+SA Sbjct: 225 KADYISTVSNFPKGFQHHAPKNFQWLRAFEVFLPFTALLRVHFPNEGRLCILNAASPISA 284 Query: 236 RYTRLFCPITRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHIPADRT 295 R TR+FCPI RNFDK P+E FN Q+F ED ++VEAQ PEDLPLDL E HI AD+T Sbjct: 285 RQTRVFCPIARNFDKDQPLEPVYDFNYQIFNEDKEIVEAQYPEDLPLDLSVETHIEADQT 344 Query: 296 SIAYRQLLTELGLGRSYTS 314 SIAYR+ L LGL + YT+ Sbjct: 345 SIAYRKGLAALGLSKLYTA 363 >Q0BUD6.1 Q0BUD6_GRABC Vanillate demethylase oxygenase subunit EC=1.14.13.82 Length = 383 Score = 342 bits (876), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 171/317 (53%), Positives = 210/317 (66%), Gaps = 3/317 (0%) Query: 1 VAIADEVQDKPLAVKLLDMKLVCYRSEGKVVIARDLCFHRGAPLSKGWVENGDIVCPYHG 60 VA+A +V ++PL+ +LLD +LV YR+ ++VIA DLC HRG PLS G + I C YHG Sbjct: 67 VALARDVGEQPLSARLLDEELVIYRAGQQIVIAGDLCPHRGVPLSMGHGDGQSIACAYHG 126 Query: 61 FRYNCEGKCTAVPAHPSSKISPKLKLIVYPAVERYGLIWTCLG---SAAEQIPDFPGWED 117 FR+ EG+C VPAHP S I KL L YPA ERYGLIWTCL + + IP P W+D Sbjct: 127 FRFGAEGRCIKVPAHPGSAIPGKLHLRTYPATERYGLIWTCLRPDETGSASIPPMPHWDD 186 Query: 118 PDYINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNELVA 177 + I P DI G +GRQMEGFLDV+HF +VHT TFGD NT VP Y + Sbjct: 187 AGFQQITCPWIDIFGFAGRQMEGFLDVAHFGFVHTGTFGDPENTTVPSYIPVPTRDGFEV 246 Query: 178 EYWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLNILNCASPVSARY 237 EY S++SNY G + P PE F+WLR FRV PF A+L++ FPD GRL I+N ASPVSAR Sbjct: 247 EYRSSLSNYPHGSDQPVPEDFVWLRHFRVHLPFTATLSIDFPDHGRLVIMNAASPVSARI 306 Query: 238 TRLFCPITRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHIPADRTSI 297 TRLF PI RNFD P+++ FN +VF ED +VEAQ PE LPLD EAH+ ADR+SI Sbjct: 307 TRLFVPIARNFDTDRPIQEVYDFNRRVFEEDKAIVEAQKPECLPLDPTLEAHVMADRSSI 366 Query: 298 AYRQLLTELGLGRSYTS 314 AYR+ L E G +T+ Sbjct: 367 AYRRALRERGFSHFFTA 383 >B0VQN7.1 B0VQN7_ACIBS Putative vanillate O-demethylase oxygenase subunit Length = 351 Score = 341 bits (874), Expect = 6e-92, Method: Compositional matrix adjust. Identities = 165/318 (51%), Positives = 213/318 (66%), Gaps = 5/318 (1%) Query: 1 VAIADEVQDKPLAVKLLDMKLVCYRSE-GKVVIARDLCFHRGAPLSKGWVENGDIVCPYH 59 VA +V P VKLLD+ + Y++E G++ + RD+C HRG PL+KGWV+ +IVCPYH Sbjct: 33 VARIQDVSTAPQRVKLLDVNMALYKTESGEIHLVRDICPHRGVPLTKGWVDGEEIVCPYH 92 Query: 60 GFRYNCEGKCTAVPAHPS-SKISPKLKLIVYPAVERYGLIWTCLGS---AAEQIPDFPGW 115 G RYN EGKCT +PA P +KIS + L +P V+RYGLIWT + A IP W Sbjct: 93 GLRYNTEGKCTQIPAQPELTKISDRFSLTKFPVVQRYGLIWTSIHGRDIAKANIPVLDTW 152 Query: 116 EDPDYINILPPGFDIAGSSGRQMEGFLDVSHFAYVHTATFGDRNNTEVPQYKVRREGNEL 175 +D ++ ILPP DI GSSGRQ+EGF+DV+HFA+VH F +R+N VP+Y R L Sbjct: 153 DDAEHQAILPPFVDIGGSSGRQLEGFIDVAHFAWVHHNAFANRDNPIVPKYHTERTNYGL 212 Query: 176 VAEYWSTVSNYGKGQENPAPEGFMWLREFRVFPPFAASLTVYFPDEGRLNILNCASPVSA 235 Y S VSNY ++ PEGF+W R F V+PPF+A LTV FP++G L ILN P+S+ Sbjct: 213 KTVYISNVSNYPHELKHLEPEGFLWKRTFEVYPPFSAVLTVDFPEDGILKILNACCPISS 272 Query: 236 RYTRLFCPITRNFDKSAPVEDTIKFNLQVFAEDADMVEAQTPEDLPLDLQAEAHIPADRT 295 TRLF P+TRNFD++ +E FN Q+FAED DMVE+Q PE+LPLDL EAH ADR+ Sbjct: 273 NKTRLFVPLTRNFDQTGDLEKVYAFNAQIFAEDQDMVESQKPEELPLDLMMEAHFEADRS 332 Query: 296 SIAYRQLLTELGLGRSYT 313 S YR++L E GL + YT Sbjct: 333 STTYRRILAEWGLSKRYT 350 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,079,773,554 Number of extensions: 93728432 Number of successful extensions: 162428 Number of sequences better than 1.0e-05: 1000 Number of HSP's gapped: 161558 Number of HSP's successfully gapped: 1001 Length of query: 314 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 179 Effective length of database: 1,788,859,178 Effective search space: 320205792862 Effective search space used: 320205792862 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)