BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0012.p (462 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3QTU2.1 B3QTU2_CHLT3 Fe-only nitrogenase, beta subunit EC=1.18.6.1 780 0.0 Q2RUK2.1 Q2RUK2_RHORT Nitrogenase EC=1.18.6.1 760 0.0 Q46C94.1 Q46C94_METBF Nitrogenase, subunit K 753 0.0 C0AC98.1 C0AC98_9BACT Nitrogenase EC=1.18.6.1 750 0.0 B9DYP8.1 B9DYP8_CLOK1 Putative uncharacterized protein 748 0.0 >B3QTU2.1 B3QTU2_CHLT3 Fe-only nitrogenase, beta subunit EC=1.18.6.1 Length = 462 Score = 780 bits (2015), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/462 (79%), Positives = 413/462 (89%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M CE+ K R GVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLL SQH K++ Sbjct: 1 MYCEVKPKARAGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HEDGAVFGALNRVEEAVDVLLMRYP+VKVVPII+TCSTEVIGDD+DGV++KL Sbjct: 61 FEIASSSLHEDGAVFGALNRVEEAVDVLLMRYPEVKVVPIISTCSTEVIGDDIDGVVIKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N+GLLKEKY REVHLI +HTPSF+GS VSGYDVAV FVK+FA KG+PNGK+NLITGWV Sbjct: 121 NDGLLKEKYPDREVHLIPIHTPSFIGSMVSGYDVAVRDFVKYFAEKGEPNGKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV ALKHLL++MQ+DATVLFEIESFDSP++PDKS SHGSTTI D+ Sbjct: 181 NPGDVVALKHLLSQMQIDATVLFEIESFDSPLMPDKSAVSHGSTTIEDLKSTANAIGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGAKAA+YLE+EFG+PA+IGPTPIGIRNTD FLQN++K+TGKPIP+SLVRERGIAI Sbjct: 241 LNRYEGAKAAQYLENEFGVPAIIGPTPIGIRNTDAFLQNLKKMTGKPIPQSLVRERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DALADL HMFLADKKVAIYG+PDLV+GLA FC+DLEM+PVLLLLGDDN TY DPR++AL Sbjct: 301 DALADLTHMFLADKKVAIYGNPDLVLGLAAFCLDLEMKPVLLLLGDDNLTYADDPRVKAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q++V++ MEIV NAD ELENRIKN+GLELDLILGHSKGR+ AIDN IPM+RVGFPTYDR Sbjct: 361 QEHVDYKMEIVTNADFWELENRIKNEGLELDLILGHSKGRYVAIDNKIPMLRVGFPTYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AG+YRHP VGYAGA LAE++ANTLF DMEYK +KEWIL+VW Sbjct: 421 AGMYRHPVVGYAGATWLAEQMANTLFADMEYKHHKEWILNVW 462 >Q2RUK2.1 Q2RUK2_RHORT Nitrogenase EC=1.18.6.1 Length = 462 Score = 760 bits (1962), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/462 (78%), Positives = 408/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ +KER G INPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLL SQH K++ Sbjct: 1 MSCELRKKERIGTINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLISQHLKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSSVHEDGAVFGAL+RVE+AVDVLLMRYPDVKV+PIITTCSTEVIGDDVDGV+ KL Sbjct: 61 FEIASSSVHEDGAVFGALDRVEQAVDVLLMRYPDVKVIPIITTCSTEVIGDDVDGVVRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 GLLKEK+ REVHLI +HTPSFVGS V+GYDVAV FVK FA+KG+P+ K+NLITGWV Sbjct: 121 EEGLLKEKFPDREVHLIPIHTPSFVGSMVTGYDVAVRDFVKAFAKKGKPSLKINLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDV LKHLLAEM+V+A VLFEIESFDSP++PDKS SHGSTTI D+ Sbjct: 181 NPGDVKELKHLLAEMRVEANVLFEIESFDSPLMPDKSAVSHGSTTIDDLIDTANAQGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEGAKAA++L+ F +PAVIGPTPIGIRNTDTFLQN++ +TGK IP SLVRERGIAI Sbjct: 241 LNRYEGAKAAEFLQKGFDVPAVIGPTPIGIRNTDTFLQNLKIMTGKAIPPSLVRERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DAL DLVHMFLADKKVAIYG+PDLVIGLAEFC+DLEM+P+LLLLGD+N Y++DPRI+AL Sbjct: 301 DALTDLVHMFLADKKVAIYGNPDLVIGLAEFCLDLEMKPMLLLLGDENAGYETDPRIKAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+ MEI+ NADL ELE+R+KNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR Sbjct: 361 QERVDHDMEIITNADLWELEDRVKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYRHP VGYAGA LAE +ANTLFTDMEYKKNKEWIL+VW Sbjct: 421 AGLYRHPVVGYAGATWLAEAMANTLFTDMEYKKNKEWILNVW 462 >Q46C94.1 Q46C94_METBF Nitrogenase, subunit K Length = 462 Score = 753 bits (1944), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/462 (77%), Positives = 413/462 (89%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ Q+ER G+INPIFTCQP GAQYAS+GIKDCI IVHGGQGCVMFVR+LF+QHFKD+ Sbjct: 1 MSCELKQRERPGIINPIFTCQPCGAQYASVGIKDCIAIVHGGQGCVMFVRMLFAQHFKDS 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F IASSS+HEDGAVFGALNRVEEAVDVLLMRYP VKV+PIITTCSTEVIGDD+DG+I KL Sbjct: 61 FEIASSSLHEDGAVFGALNRVEEAVDVLLMRYPHVKVIPIITTCSTEVIGDDIDGLIRKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N LLKEKYA REVHLI +HTPSF GS ++GYDVAV VK FA+K +PNGKLNLITGWV Sbjct: 121 NKKLLKEKYANREVHLIPIHTPSFKGSMITGYDVAVHDIVKEFAKKDKPNGKLNLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHLL+EM +DATVLFEIESFDSP++PDKSG +HGSTTI D+TG Sbjct: 181 NPGDVTALKHLLSEMDIDATVLFEIESFDSPLMPDKSGFAHGSTTIEDLTGTADALGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 KYEG KAA+YL+++F +PA+IGPTPIGIRNTDTFLQN++K+TGKPIPESLVRERGIAI Sbjct: 241 LNKYEGGKAAEYLKNKFDVPAIIGPTPIGIRNTDTFLQNLKKMTGKPIPESLVRERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ DL HMF ADKKVAIYG+PDLVIGLAEFC+D+EM+PV+LLLGDDN TYK DPRI+ Sbjct: 301 DAITDLTHMFFADKKVAIYGNPDLVIGLAEFCLDMEMKPVVLLLGDDNNTYKDDPRIKEF 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+ V+F M+I++NADL ELE RIK+K +ELDLILGHSKGR+TAIDNNIPMVRVGFPT+DR Sbjct: 361 QEKVDFDMQIIMNADLWELERRIKDKEVELDLILGHSKGRWTAIDNNIPMVRVGFPTFDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYRHP VGY GAI LAE++ANTLFTDME+KK++EWIL+VW Sbjct: 421 AGLYRHPVVGYEGAIWLAEQMANTLFTDMEFKKDREWILNVW 462 >C0AC98.1 C0AC98_9BACT Nitrogenase EC=1.18.6.1 Length = 462 Score = 750 bits (1937), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/462 (76%), Positives = 407/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M CE+ K+R G+INPIFTCQPAGAQ+ASIGIKDCIGIVHGGQGCVMFVRL+FSQHFK++ Sbjct: 1 MLCELKPKDRVGIINPIFTCQPAGAQFASIGIKDCIGIVHGGQGCVMFVRLIFSQHFKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F +ASSSVHE+GAVFGA RVEEAVDVLLMRYPDVKVVPII+TC TEVIGDD+DGV+ KL Sbjct: 61 FELASSSVHEEGAVFGATRRVEEAVDVLLMRYPDVKVVPIISTCCTEVIGDDIDGVVTKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N GLL EKY GREVHLI +HTPSFVGS VSGYDVAV FV HFA+K +P+GKLNL+TGWV Sbjct: 121 NRGLLAEKYPGREVHLIPIHTPSFVGSMVSGYDVAVRDFVTHFAKKTEPSGKLNLLTGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHL+AEMQ+DATVLFEIE FDSP++P + SHG+TTIADM G Sbjct: 181 NPGDVTALKHLVAEMQIDATVLFEIEGFDSPLMPYGNFVSHGNTTIADMQGTANALGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YE +KAA+YLE EFGIP +IGPTPIGIRNTDTFLQN++KLTGKPIPESLVRERG+A+ Sbjct: 241 LNRYECSKAAQYLESEFGIPTIIGPTPIGIRNTDTFLQNLKKLTGKPIPESLVRERGVAL 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ D+ HMFLA+KKVAIYG+PDLVIGLAEFC+DLEM+PVLLLLGDDN Y DPRI+AL Sbjct: 301 DAITDVTHMFLAEKKVAIYGNPDLVIGLAEFCLDLEMKPVLLLLGDDNAFYGDDPRIKAL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 ++NV+F MEIV NADL +LE+RIK +ELD+ILGHSKGRF +I+ NIPM+RVGFPTYDR Sbjct: 361 KENVDFDMEIVTNADLWDLESRIKEGKIELDMILGHSKGRFISIEYNIPMLRVGFPTYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AGLYR+P VGYAGA+ LAE +ANTLFTDMEYKKN+EWIL++W Sbjct: 421 AGLYRYPVVGYAGAMWLAEAMANTLFTDMEYKKNREWILNMW 462 >B9DYP8.1 B9DYP8_CLOK1 Putative uncharacterized protein Length = 462 Score = 748 bits (1932), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/462 (74%), Positives = 410/462 (88%) Query: 1 MTCEIVQKERGGVINPIFTCQPAGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKDN 60 M+CE+ +KER G+INPIFTCQP GAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFK++ Sbjct: 1 MSCEVKEKERAGIINPIFTCQPCGAQYASIGIKDCIGIVHGGQGCVMFVRLLFSQHFKES 60 Query: 61 FLIASSSVHEDGAVFGALNRVEEAVDVLLMRYPDVKVVPIITTCSTEVIGDDVDGVILKL 120 F +ASSS+HED AVFGAL R+EEAVDVLLMRYP +KVVPIITTCSTE+IGDD+DGV+ KL Sbjct: 61 FELASSSLHEDAAVFGALRRIEEAVDVLLMRYPHIKVVPIITTCSTEIIGDDIDGVVNKL 120 Query: 121 NNGLLKEKYAGREVHLIAVHTPSFVGSQVSGYDVAVEAFVKHFARKGQPNGKLNLITGWV 180 N GLLK+K+AGREV+L+ +HTPSF GS VSGY++AV+ FVK+FA+KG+PNGK+NLITGWV Sbjct: 121 NRGLLKKKFAGREVYLVPIHTPSFTGSMVSGYNIAVKDFVKYFAKKGEPNGKVNLITGWV 180 Query: 181 NPGDVTALKHLLAEMQVDATVLFEIESFDSPILPDKSGESHGSTTIADMTGXXXXXXXXX 240 NPGDVTALKHLL+EM+VDATVLFEIESFDSP++P+ + SHG+TT+ D+TG Sbjct: 181 NPGDVTALKHLLSEMKVDATVLFEIESFDSPLMPEGNTVSHGNTTVEDLTGTANAIGTIA 240 Query: 241 XXKYEGAKAAKYLEDEFGIPAVIGPTPIGIRNTDTFLQNVRKLTGKPIPESLVRERGIAI 300 +YEG +AA+YLE +FG+ AVIGPTPIGIRNTD FL+N+ +TGKPIP+SLV ERGIAI Sbjct: 241 LNRYEGGQAAEYLEKKFGVSAVIGPTPIGIRNTDIFLKNLSTMTGKPIPKSLVHERGIAI 300 Query: 301 DALADLVHMFLADKKVAIYGHPDLVIGLAEFCIDLEMQPVLLLLGDDNTTYKSDPRIQAL 360 DA+ D+ HMFLADKKVAIYG DLVIGLA+FC+D+EM+P+LLLLGDDN +Y DPRI+ L Sbjct: 301 DAITDVAHMFLADKKVAIYGSADLVIGLAQFCLDMEMKPMLLLLGDDNKSYIKDPRIEEL 360 Query: 361 QQNVEFGMEIVLNADLLELENRIKNKGLELDLILGHSKGRFTAIDNNIPMVRVGFPTYDR 420 Q+NV++ MEI+ NADL ELE+RIKNKGL+LDLILGHSKGRF +IDNNIPM+RVGFP YDR Sbjct: 361 QKNVDYDMEIITNADLWELEDRIKNKGLKLDLILGHSKGRFISIDNNIPMLRVGFPVYDR 420 Query: 421 AGLYRHPTVGYAGAIRLAEEIANTLFTDMEYKKNKEWILSVW 462 AG YR+P +GYAGAI LAE IANT+FTDMEY+KN+EWIL+VW Sbjct: 421 AGFYRYPIMGYAGAIWLAEAIANTIFTDMEYQKNREWILNVW 462 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,772,512,166 Number of extensions: 118027824 Number of successful extensions: 261536 Number of sequences better than 1.0e-05: 530 Number of HSP's gapped: 259768 Number of HSP's successfully gapped: 549 Length of query: 462 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 323 Effective length of database: 1,751,175,118 Effective search space: 565629563114 Effective search space used: 565629563114 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)