BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0010.p (518 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0AC96.1 C0AC96_9BACT Nitrogenase protein alpha chain EC=1.18.6.1 942 0.0 B3QTU0.1 B3QTU0_CHLT3 Nitrogenase protein alpha chain EC=1.18.6.1 936 0.0 Q2RUK0.1 Q2RUK0_RHORT Nitrogenase protein alpha chain EC=1.18.6.1 927 0.0 B9DYP6.1 B9DYP6_CLOK1 Nitrogenase protein alpha chain EC=1.18.6.1 912 0.0 A5N543.1 A5N543_CLOK5 Nitrogenase protein alpha chain EC=1.18.6.1 912 0.0 >C0AC96.1 C0AC96_9BACT Nitrogenase protein alpha chain EC=1.18.6.1 Length = 526 Score = 942 bits (2436), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/510 (86%), Positives = 474/510 (92%), Gaps = 1/510 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+H F CS CIPER +HAVIKGPGEDL+ ALP GYLNTIPGTISERGCAYCGAKHVIGT Sbjct: 1 MPYHEFNCSKCIPERKKHAVIKGPGEDLSMALPLGYLNTIPGTISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIHLSHGPVGCTYDTWQTKRYISDN NFQ++ TFA+D+KEKH++FGAE +LK+NII Sbjct: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNKNFQIEYTFATDMKEKHVIFGAEKMLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCA+ALIGDD+ AVAQEVMDEMPDVDIFVCNSPGF GPSQSGGH Sbjct: 121 EAFKAFPDIKRMTIYQTCASALIGDDLAAVAQEVMDEMPDVDIFVCNSPGFGGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWINQKVG +EP+ITSDYVINYVGEYNIQGDQEVM DYFK MGIQVLSTFTGNGS Sbjct: 181 HKINIAWINQKVGNIEPKITSDYVINYVGEYNIQGDQEVMLDYFKRMGIQVLSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD+LR+MHRAHLNVLECARSAEYICNELR +YGIPRLDIDGFGF PLSASLRKIGLFFGI Sbjct: 241 YDELRSMHRAHLNVLECARSAEYICNELRKRYGIPRLDIDGFGFGPLSASLRKIGLFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 E+RAQAIIDE+TARWKPELDWYKERLQGK+VCLWPGGSKLWHWA+VIH EMGVEVVS+YT Sbjct: 301 EERAQAIIDEETARWKPELDWYKERLQGKKVCLWPGGSKLWHWAHVIHAEMGVEVVSVYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGI+RCE+GALAIDDPNELEGLEAM+MLKPD+IFTGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGISRCEEGALAIDDPNELEGLEAMDMLKPDVIFTGKRPGEVAKKLRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKG+EGWVRFARDIYNA+YSPIHQLS L+ISKDEIPTDKGF T+RM+SDV Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNAIYSPIHQLSYLDISKDEIPTDKGFVTRRMISDVK 480 Query: 481 LX-XXXXXXXLRQYTGGYDSVSKLRNKTYP 509 L LRQYTG D V+ LR KTYP Sbjct: 481 LSEEVRTSTTLRQYTGKMDCVTPLRKKTYP 510 >B3QTU0.1 B3QTU0_CHLT3 Nitrogenase protein alpha chain EC=1.18.6.1 Length = 514 Score = 936 bits (2419), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/513 (85%), Positives = 473/513 (92%), Gaps = 1/513 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+H F+CS CIPER HAVIKGPGEDLTSALP GYLNTIPG+ISERGCAYCGAKHVIGT Sbjct: 1 MPYHEFECSKCIPERKMHAVIKGPGEDLTSALPLGYLNTIPGSISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGPVGCTYDTWQTKRYISDN NFQLK T+A+DVKEKHIVFGAE LLK+NII Sbjct: 61 PMKDVIHISHGPVGCTYDTWQTKRYISDNDNFQLKYTYATDVKEKHIVFGAEKLLKQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCATALIGDDI A+AQEVM+EMPDVDIFVCNSPGFAGPSQSGGH Sbjct: 121 EAFRAFPDIKRMTIYQTCATALIGDDIDAIAQEVMEEMPDVDIFVCNSPGFAGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAW+N+KVGTVEPEITSDYVINYVGEYNIQGDQEVM DYF MGIQVLSTFTGNG+ Sbjct: 181 HKINIAWVNRKVGTVEPEITSDYVINYVGEYNIQGDQEVMLDYFNRMGIQVLSTFTGNGT 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LR+MH+AHLNVLECARSAEYICNELRV+YGIPRLDIDGFGFEPLSASL K+G FFGI Sbjct: 241 YDGLRSMHKAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSASLLKVGRFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRAQAIIDE+TARWKPELDWYKERL GK+VCLWPGGSKLWHWA+VIH+EMGV+VVS+YT Sbjct: 301 EDRAQAIIDEETARWKPELDWYKERLNGKKVCLWPGGSKLWHWAHVIHDEMGVDVVSVYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARCE G LAIDDPNELEGLEAME LKPDIIFTGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCEVGTLAIDDPNELEGLEAMEKLKPDIIFTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGP+KG+EGWVRFARDIYNA+YSPIHQLS L+ISKDEIPT+ GF TQRM+SD Sbjct: 421 NAHAYHNGPWKGFEGWVRFARDIYNAIYSPIHQLSGLDISKDEIPTEHGFVTQRMISDAT 480 Query: 481 LXXXXXXXXL-RQYTGGYDSVSKLRNKTYPHLE 512 L + R+YTG YD ++ LR KTYP L+ Sbjct: 481 LSHEVRTSAMVREYTGKYDPIADLRKKTYPDLK 513 >Q2RUK0.1 Q2RUK0_RHORT Nitrogenase protein alpha chain EC=1.18.6.1 Length = 519 Score = 927 bits (2395), Expect = 0.0, Method: Compositional matrix adjust. Identities = 435/513 (84%), Positives = 468/513 (91%), Gaps = 1/513 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MPHHTFKCS CIPERT+HAVIKG EDLTS LP GYLNTIPG+ISERGCAYCGAKHVIG Sbjct: 1 MPHHTFKCSECIPERTKHAVIKGADEDLTSCLPLGYLNTIPGSISERGCAYCGAKHVIGQ 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKDVIH+SHGP+GCTYDTWQTKRYISDN NFQLK T+A+DV+EKHIVFGAE LLK+NI+ Sbjct: 61 PMKDVIHISHGPIGCTYDTWQTKRYISDNNNFQLKYTYATDVREKHIVFGAEKLLKQNIL 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRMTIY TCATALIGDDI A+A EVMDE+PDVDIFVCNSPGFAGPSQSGGH Sbjct: 121 EAFKAFPDIKRMTIYQTCATALIGDDIDAIASEVMDELPDVDIFVCNSPGFAGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAW+NQKVGTVEPEITSDYVINYVGEYNIQGDQEVM DYF MGIQ+LSTFTGNGS Sbjct: 181 HKINIAWVNQKVGTVEPEITSDYVINYVGEYNIQGDQEVMQDYFNRMGIQILSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YD LRAMHRAHLNVLECARSAEYICNELRV+YGIPRLDIDGFGFEPLS SLRKIGLFFGI Sbjct: 241 YDGLRAMHRAHLNVLECARSAEYICNELRVRYGIPRLDIDGFGFEPLSDSLRKIGLFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 EDRAQAIIDE+TA+WKP+LDWYKERL+GK+VCLWPGGSKLWHWA+VI EEMG+ VVSLYT Sbjct: 301 EDRAQAIIDEETAKWKPQLDWYKERLRGKKVCLWPGGSKLWHWAHVIQEEMGLNVVSLYT 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGDMEKGIARC +GALAIDDPNELEGLEA+EMLKPDII TGKRPGEVAKK+RVPYL Sbjct: 361 KFGHQGDMEKGIARCGEGALAIDDPNELEGLEALEMLKPDIILTGKRPGEVAKKVRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKGYEGWVRFARDIYNA+YSPI QLSAL+ISKD IPTD+GF T +M+SD Sbjct: 421 NAHAYHNGPYKGYEGWVRFARDIYNAIYSPIFQLSALDISKDPIPTDQGFLTPQMISDPA 480 Query: 481 L-XXXXXXXXLRQYTGGYDSVSKLRNKTYPHLE 512 L L Y G YD++S LR KTYP + Sbjct: 481 LPAEVRSSTVLTPYRGAYDTISALREKTYPRFD 513 >B9DYP6.1 B9DYP6_CLOK1 Nitrogenase protein alpha chain EC=1.18.6.1 Length = 516 Score = 912 bits (2356), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/510 (83%), Positives = 468/510 (91%), Gaps = 1/510 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+H F+CS CIPER QHAVIKGPGE+LT ALP GYLNTIPGTISERGCAYCGAKHVIGT Sbjct: 1 MPYHEFECSKCIPERKQHAVIKGPGENLTDALPLGYLNTIPGTISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKD IH+SHGPVGCTYDTWQTKRYIS+N NFQ+K T+A+D+KEKH+VFGAE LL++NII Sbjct: 61 PMKDAIHMSHGPVGCTYDTWQTKRYISNNDNFQIKYTYATDMKEKHVVFGAEKLLRQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRM IY TCA+ALIGDDI AVAQ+VM EMP VDIFVCNSPGF GPSQSGGH Sbjct: 121 EAFKAFPDIKRMCIYQTCASALIGDDINAVAQKVMKEMPGVDIFVCNSPGFGGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWI+QKVGT+EPEI SDYVINYVGEYNIQGD+EVM DYFK MGIQ+LSTFTGNGS Sbjct: 181 HKINIAWIDQKVGTLEPEIKSDYVINYVGEYNIQGDEEVMMDYFKRMGIQILSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YDDLR+MH+AHLNVLECARSAEYICNELR +YGIPRLDIDGFGF PLS SLRKIGLFFGI Sbjct: 241 YDDLRSMHKAHLNVLECARSAEYICNELRKRYGIPRLDIDGFGFGPLSLSLRKIGLFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 ED+AQAIIDE+TA+WKPELDWYKERL+GK+VCLW GGSKLWHWA+ IHEEMGVEVVS+Y+ Sbjct: 301 EDKAQAIIDEETAKWKPELDWYKERLKGKKVCLWSGGSKLWHWAHAIHEEMGVEVVSVYS 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGD EKGIARCE+GALAIDDPNELEGLEAMEMLKPD+IFTGKRPGEVAKKIRVPYL Sbjct: 361 KFGHQGDFEKGIARCEEGALAIDDPNELEGLEAMEMLKPDVIFTGKRPGEVAKKIRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKG+EGWVRFARDIYNA+YSPIH+L+ L+ISKDEIPTDKGF+TQ+M+SDVN Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNAIYSPIHKLAYLDISKDEIPTDKGFSTQKMISDVN 480 Query: 481 LXXXXXXX-XLRQYTGGYDSVSKLRNKTYP 509 L LR+YTG + + L KTYP Sbjct: 481 LSEEVVSSPDLREYTGKCNIIPDLCKKTYP 510 >A5N543.1 A5N543_CLOK5 Nitrogenase protein alpha chain EC=1.18.6.1 Length = 516 Score = 912 bits (2356), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/510 (83%), Positives = 468/510 (91%), Gaps = 1/510 (0%) Query: 1 MPHHTFKCSACIPERTQHAVIKGPGEDLTSALPEGYLNTIPGTISERGCAYCGAKHVIGT 60 MP+H F+CS CIPER QHAVIKGPGE+LT ALP GYLNTIPGTISERGCAYCGAKHVIGT Sbjct: 1 MPYHEFECSKCIPERKQHAVIKGPGENLTDALPLGYLNTIPGTISERGCAYCGAKHVIGT 60 Query: 61 PMKDVIHLSHGPVGCTYDTWQTKRYISDNGNFQLKNTFASDVKEKHIVFGAEGLLKKNII 120 PMKD IH+SHGPVGCTYDTWQTKRYIS+N NFQ+K T+A+D+KEKH+VFGAE LL++NII Sbjct: 61 PMKDAIHMSHGPVGCTYDTWQTKRYISNNDNFQIKYTYATDMKEKHVVFGAEKLLRQNII 120 Query: 121 EAFTAFPEIKRMTIYSTCATALIGDDIGAVAQEVMDEMPDVDIFVCNSPGFAGPSQSGGH 180 EAF AFP+IKRM IY TCA+ALIGDDI AVAQ+VM EMP VDIFVCNSPGF GPSQSGGH Sbjct: 121 EAFKAFPDIKRMCIYQTCASALIGDDINAVAQKVMKEMPGVDIFVCNSPGFGGPSQSGGH 180 Query: 181 HKINIAWINQKVGTVEPEITSDYVINYVGEYNIQGDQEVMADYFKSMGIQVLSTFTGNGS 240 HKINIAWI+QKVGT+EPEI SDYVINYVGEYNIQGD+EVM DYFK MGIQ+LSTFTGNGS Sbjct: 181 HKINIAWIDQKVGTLEPEIKSDYVINYVGEYNIQGDEEVMMDYFKRMGIQILSTFTGNGS 240 Query: 241 YDDLRAMHRAHLNVLECARSAEYICNELRVKYGIPRLDIDGFGFEPLSASLRKIGLFFGI 300 YDDLR+MH+AHLNVLECARSAEYICNELR +YGIPRLDIDGFGF PLS SLRKIGLFFGI Sbjct: 241 YDDLRSMHKAHLNVLECARSAEYICNELRKRYGIPRLDIDGFGFGPLSLSLRKIGLFFGI 300 Query: 301 EDRAQAIIDEQTARWKPELDWYKERLQGKRVCLWPGGSKLWHWANVIHEEMGVEVVSLYT 360 ED+AQAIIDE+TA+WKPELDWYKERL+GK+VCLW GGSKLWHWA+ IHEEMGVEVVS+Y+ Sbjct: 301 EDKAQAIIDEETAKWKPELDWYKERLKGKKVCLWSGGSKLWHWAHAIHEEMGVEVVSVYS 360 Query: 361 KFGHQGDMEKGIARCEQGALAIDDPNELEGLEAMEMLKPDIIFTGKRPGEVAKKIRVPYL 420 KFGHQGD EKGIARCE+GALAIDDPNELEGLEAMEMLKPD+IFTGKRPGEVAKKIRVPYL Sbjct: 361 KFGHQGDFEKGIARCEEGALAIDDPNELEGLEAMEMLKPDVIFTGKRPGEVAKKIRVPYL 420 Query: 421 NAHAYHNGPYKGYEGWVRFARDIYNAVYSPIHQLSALNISKDEIPTDKGFATQRMVSDVN 480 NAHAYHNGPYKG+EGWVRFARDIYNA+YSPIH+L+ L+ISKDEIPTDKGF+TQ+M+SDVN Sbjct: 421 NAHAYHNGPYKGFEGWVRFARDIYNAIYSPIHKLAYLDISKDEIPTDKGFSTQKMISDVN 480 Query: 481 LXXXXXXX-XLRQYTGGYDSVSKLRNKTYP 509 L LR+YTG + + L KTYP Sbjct: 481 LSEEVVSSPDLREYTGKCNIIPDLCKKTYP 510 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,280,008,609 Number of extensions: 142856014 Number of successful extensions: 295342 Number of sequences better than 1.0e-05: 873 Number of HSP's gapped: 292430 Number of HSP's successfully gapped: 900 Length of query: 518 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 378 Effective length of database: 1,741,754,103 Effective search space: 658383050934 Effective search space used: 658383050934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)