BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0584.0009.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C1DK95.1 C1DK95_AZOVD Nitrogenase iron protein 423 e-117 B3QTT7.1 B3QTT7_CHLT3 Nitrogenase iron protein EC=1.18.6.1 417 e-115 P09553.1 NIFH3_CLOPA Nitrogenase iron protein 3 EC=1.18.6.1 417 e-115 P16269.1 NIFH3_AZOVI Nitrogenase iron protein 3 EC=1.18.6.1 409 e-112 C0AC95.1 C0AC95_9BACT Nitrogenase iron protein EC=1.18.6.1 407 e-112 >C1DK95.1 C1DK95_AZOVD Nitrogenase iron protein Length = 275 Score = 423 bits (1088), Expect = e-117, Method: Compositional matrix adjust. Identities = 211/275 (76%), Positives = 223/275 (81%) Query: 1 MTRXXXXXXXXXXXXSTTQQNTAAAMAYFHDKKVFIHGCDPKADSTRMILGGMNQKTLMD 60 MTR STT QNTAAA+AYFHDKKVFIHGCDPKADSTR+ILGG Q+TLMD Sbjct: 1 MTRKVAIYGKGGIGKSTTTQNTAAALAYFHDKKVFIHGCDPKADSTRLILGGKPQETLMD 60 Query: 61 MLRDDGAENITTEKVVKEGYLGIQCVESGGPEPGVGCAGRGVITAIDLMESNGAYTXXXX 120 MLRD GAE IT + V+K+G+L IQCVESGGPEPGVGCAGRGVITAIDLME NGAYT Sbjct: 61 MLRDKGAEKITNDDVIKKGFLDIQCVESGGPEPGVGCAGRGVITAIDLMEENGAYTDDLD 120 Query: 121 XXXXXXXXXXXXXXXAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG 180 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG Sbjct: 121 FVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG 180 Query: 181 GIICNSRNVDREREFLEEFTASIGTQMIHFMPRDNIVQKAEFNKKTVVEFDAESKQAGEY 240 GIICNSR VD EREFLEEFTA+IGT+MIHF+PRDNIVQKAEFNKKTV EF E QA EY Sbjct: 181 GIICNSRKVDGEREFLEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFAPEENQAKEY 240 Query: 241 GELGRKIIENENFVIPKPLSMDQLEAMVVKYGIGD 275 GEL RKIIEN+ FVIPKPL+MDQLE MVVKYGI D Sbjct: 241 GELARKIIENDEFVIPKPLTMDQLEDMVVKYGIAD 275 >B3QTT7.1 B3QTT7_CHLT3 Nitrogenase iron protein EC=1.18.6.1 Length = 274 Score = 417 bits (1073), Expect = e-115, Method: Compositional matrix adjust. Identities = 201/260 (77%), Positives = 226/260 (86%) Query: 16 STTQQNTAAAMAYFHDKKVFIHGCDPKADSTRMILGGMNQKTLMDMLRDDGAENITTEKV 75 STTQQNTAAAMAYFH++KVFIHGCDPKADSTRMILGGMNQ T+MD+LRD+GAE IT +K+ Sbjct: 15 STTQQNTAAAMAYFHNQKVFIHGCDPKADSTRMILGGMNQATIMDVLRDEGAEKITADKI 74 Query: 76 VKEGYLGIQCVESGGPEPGVGCAGRGVITAIDLMESNGAYTXXXXXXXXXXXXXXXXXXX 135 +K G+ I+CVESGGPEPGVGCAGRGVITAID+ME+ GAY+ Sbjct: 75 IKGGFGNIRCVESGGPEPGVGCAGRGVITAIDMMEATGAYSSDLDYVFFDVLGDVVCGGF 134 Query: 136 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLGGIICNSRNVDREREF 195 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLGGIICNSR VDREREF Sbjct: 135 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLGGIICNSRKVDREREF 194 Query: 196 LEEFTASIGTQMIHFMPRDNIVQKAEFNKKTVVEFDAESKQAGEYGELGRKIIENENFVI 255 LEEFTA+IGTQMIHF+PRDN+VQKAEFN+KTVVEFD E KQA EYGEL RKIIEN+ VI Sbjct: 195 LEEFTAAIGTQMIHFVPRDNVVQKAEFNRKTVVEFDPECKQAKEYGELARKIIENKMLVI 254 Query: 256 PKPLSMDQLEAMVVKYGIGD 275 PKPL++++LE+MV+KYG+ D Sbjct: 255 PKPLNINELESMVLKYGLVD 274 >P09553.1 NIFH3_CLOPA Nitrogenase iron protein 3 EC=1.18.6.1 Length = 275 Score = 417 bits (1072), Expect = e-115, Method: Compositional matrix adjust. Identities = 209/275 (76%), Positives = 221/275 (80%) Query: 1 MTRXXXXXXXXXXXXSTTQQNTAAAMAYFHDKKVFIHGCDPKADSTRMILGGMNQKTLMD 60 MTR STTQQNTAAAMA+F+DKKVFIHGCDPKADSTR+ILGGM QKTLMD Sbjct: 1 MTRKIAIYGKGGIGKSTTQQNTAAAMAHFYDKKVFIHGCDPKADSTRLILGGMPQKTLMD 60 Query: 61 MLRDDGAENITTEKVVKEGYLGIQCVESGGPEPGVGCAGRGVITAIDLMESNGAYTXXXX 120 MLRD+G E ITTE +V+ GY I+CVESGGPEPGVGCAGRGVITAIDLME NGAYT Sbjct: 61 MLRDEGEEKITTENIVRVGYEDIRCVESGGPEPGVGCAGRGVITAIDLMEKNGAYTEDLD 120 Query: 121 XXXXXXXXXXXXXXXAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG 180 AMPIRDGKAQEVYIVASGEMMA+YAANNICKGLVKYA QSGVRLG Sbjct: 121 FVFFDVLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAVYAANNICKGLVKYANQSGVRLG 180 Query: 181 GIICNSRNVDREREFLEEFTASIGTQMIHFMPRDNIVQKAEFNKKTVVEFDAESKQAGEY 240 GIICNSR VD EREF+EEF ASIGTQMIHFMPRDNIVQKAEFNK+TV+EFD QA EY Sbjct: 181 GIICNSRMVDLEREFIEEFAASIGTQMIHFMPRDNIVQKAEFNKQTVIEFDDTCNQAKEY 240 Query: 241 GELGRKIIENENFVIPKPLSMDQLEAMVVKYGIGD 275 GEL RKIIENE FVIP PL MD LEAMVVKYG+ D Sbjct: 241 GELARKIIENEMFVIPTPLKMDDLEAMVVKYGMTD 275 >P16269.1 NIFH3_AZOVI Nitrogenase iron protein 3 EC=1.18.6.1 Length = 275 Score = 409 bits (1052), Expect = e-112, Method: Compositional matrix adjust. Identities = 204/275 (74%), Positives = 219/275 (79%) Query: 1 MTRXXXXXXXXXXXXSTTQQNTAAAMAYFHDKKVFIHGCDPKADSTRMILGGMNQKTLMD 60 MTR STT QNTAAA+AYFHDKKVF HGCDPKADSTR+ILGG ++TLMD Sbjct: 1 MTRKVAIYGKGGIGKSTTTQNTAAALAYFHDKKVFTHGCDPKADSTRLILGGKPEETLMD 60 Query: 61 MLRDDGAENITTEKVVKEGYLGIQCVESGGPEPGVGCAGRGVITAIDLMESNGAYTXXXX 120 M+RD GAE IT + V+K+G+L IQCVESGGPEPGVGCAGRGVITAIDLME NGAYT Sbjct: 61 MVRDKGAEKITNDDVIKKGFLDIQCVESGGPEPGVGCAGRGVITAIDLMEENGAYTDDLD 120 Query: 121 XXXXXXXXXXXXXXXAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLG 180 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQS V LG Sbjct: 121 FVFFDDLGDVVCGGFAMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSAVGLG 180 Query: 181 GIICNSRNVDREREFLEEFTASIGTQMIHFMPRDNIVQKAEFNKKTVVEFDAESKQAGEY 240 GIICNSR VD ERE +EEFTA+IGT+MIHF+PRDNIVQKAEFNKKTV EF E QA EY Sbjct: 181 GIICNSRKVDGERESVEEFTAAIGTKMIHFVPRDNIVQKAEFNKKTVTEFAPEENQAKEY 240 Query: 241 GELGRKIIENENFVIPKPLSMDQLEAMVVKYGIGD 275 GEL RKIIEN+ FVIPKPL+MDQLE MVVKYGI D Sbjct: 241 GELARKIIENDEFVIPKPLTMDQLEDMVVKYGIAD 275 >C0AC95.1 C0AC95_9BACT Nitrogenase iron protein EC=1.18.6.1 Length = 274 Score = 407 bits (1045), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/260 (74%), Positives = 219/260 (84%) Query: 16 STTQQNTAAAMAYFHDKKVFIHGCDPKADSTRMILGGMNQKTLMDMLRDDGAENITTEKV 75 STT QNTAAAMAYFH+KK+FIHGCDPKADSTR+ILGG Q+TLMD+LR+ GAE +T EKV Sbjct: 15 STTTQNTAAAMAYFHNKKIFIHGCDPKADSTRLILGGKPQETLMDVLREQGAEKVTNEKV 74 Query: 76 VKEGYLGIQCVESGGPEPGVGCAGRGVITAIDLMESNGAYTXXXXXXXXXXXXXXXXXXX 135 +K G+ I CVESGGPEPGVGCAGRGVITAIDLME+NGAYT Sbjct: 75 IKVGFKDIHCVESGGPEPGVGCAGRGVITAIDLMEANGAYTPDLDFVFYDVLGDVVCGGF 134 Query: 136 AMPIRDGKAQEVYIVASGEMMAIYAANNICKGLVKYAKQSGVRLGGIICNSRNVDREREF 195 AMPIRDGKAQEVYIVASGEMMA+YAANNICKGL+K+AKQSGVRLGGIICNSR VDREREF Sbjct: 135 AMPIRDGKAQEVYIVASGEMMAVYAANNICKGLLKFAKQSGVRLGGIICNSRKVDREREF 194 Query: 196 LEEFTASIGTQMIHFMPRDNIVQKAEFNKKTVVEFDAESKQAGEYGELGRKIIENENFVI 255 ++EFT++IGT MIHF+PRDN+VQ+AEFNKKTV E+D QA EY EL RKIIEN+NFVI Sbjct: 195 IDEFTSAIGTHMIHFVPRDNVVQRAEFNKKTVTEYDPSDNQANEYSELARKIIENKNFVI 254 Query: 256 PKPLSMDQLEAMVVKYGIGD 275 PKPL+MD+LE MVVKYG+ + Sbjct: 255 PKPLTMDELEKMVVKYGLAE 274 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.134 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,365,783,807 Number of extensions: 50516037 Number of successful extensions: 140187 Number of sequences better than 1.0e-05: 10899 Number of HSP's gapped: 122653 Number of HSP's successfully gapped: 10901 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)