BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0041.p (368 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9E3.1 B3K9E3_9BACI Fatty acid desaturase 520 e-146 C0Z4P2.1 C0Z4P2_BREBN Fatty acid desaturase EC=1.14.99.- 488 e-136 A8VS98.1 A8VS98_9BACI Peptidase M23B 481 e-134 A7Z1Q4.1 A7Z1Q4_BACA2 Putitative fatty acid desaturase 465 e-129 Q65N39.1 Q65N39_BACLD Putative uncharacterized protein 460 e-128 >B3K9E3.1 B3K9E3_9BACI Fatty acid desaturase Length = 358 Score = 520 bits (1339), Expect = e-146, Method: Compositional matrix adjust. Identities = 241/356 (67%), Positives = 295/356 (82%), Gaps = 5/356 (1%) Query: 18 RQTSQLSSLKKSMAPFEKTNQRASIRQLINTLVPLVLLWMAAYFSLQVSYGLTLLFAVPA 77 + ++LSSLKK++AP+E+ + +ASI QL NTLVPL+LLW AAY SL VSY LT+ AV A Sbjct: 3 KPDNKLSSLKKNVAPYEEIDTKASIGQLFNTLVPLILLWYAAYLSLSVSYWLTIPIAVVA 62 Query: 78 AGFVIRTFIIFHDCCHGSFFNNRKANDIIGTITGVLTLVPYRQWKHSHSIHHAGSSNLDK 137 +GFV+RTFIIFHDCCHGSFF +RKAN I+GTITGVLTL PY QWK++H+IHHA SSNLDK Sbjct: 63 SGFVVRTFIIFHDCCHGSFFKSRKANKILGTITGVLTLCPYEQWKNTHAIHHATSSNLDK 122 Query: 138 RGIGDIWIMTVEEYLAAKPLKRLYYRIYRNPLIMFGVGPIAVFLIQYRFNARGARRKERM 197 RGIGD+W++TVEEY AA +RLYYR+YRNP++MFG+GPIA+FLI YRFN +GARRKE++ Sbjct: 123 RGIGDMWVLTVEEYRAASFWQRLYYRVYRNPIVMFGLGPIAIFLISYRFNRKGARRKEKI 182 Query: 198 NTYLTNVSIALLYSTLIWAMGWQAFLLVQLPVVFVSGCLGIWLFYVQHQFEDSYFEHEEE 257 + Y TN+ I LY+ L WA+GWQAFLLVQ P+ VSG LGIWLFYVQHQFE++Y+EH+EE Sbjct: 183 SNYTTNILIVALYALLCWAIGWQAFLLVQAPIFMVSGMLGIWLFYVQHQFEETYYEHDEE 242 Query: 258 WSYVSAAVEGSSYYKLPKLLQWITGNIGFHHVHHLSPKVPNYNLELAHNASEPLQHATTI 317 WSY++AAVEGSSYYKLPK+LQWITGNIGFHHVHHLSPKVPNYNLE AH A+ PLQ ATTI Sbjct: 243 WSYINAAVEGSSYYKLPKVLQWITGNIGFHHVHHLSPKVPNYNLEKAHEAAPPLQKATTI 302 Query: 318 TIGTSLKALHFRLWDEENKGFVGFKEIKARMKQTKPAA-----EGLKVIKPGFQSE 368 T+ TSLK++ FRLWDEE K FV FKE + +++ + +A + L +P Q E Sbjct: 303 TLKTSLKSIRFRLWDEEKKVFVTFKEARRSLRKPQESALAKAEKNLASPRPRVQRE 358 >C0Z4P2.1 C0Z4P2_BREBN Fatty acid desaturase EC=1.14.99.- Length = 352 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 235/350 (67%), Positives = 286/350 (81%), Gaps = 3/350 (0%) Query: 21 SQLSSLKKSMAPFEKTNQRASIRQLINTLVPLVLLWMAAYFSLQVSYGLTLLFAVPAAGF 80 + ++ LKK++APFEK++ + SIRQLINTLVPL+LLW AY SL VSY LTL + AAGF Sbjct: 4 ANIAELKKNVAPFEKSDMKKSIRQLINTLVPLILLWYGAYLSLSVSYWLTLPIVIVAAGF 63 Query: 81 VIRTFIIFHDCCHGSFFNNRKANDIIGTITGVLTLVPYRQWKHSHSIHHAGSSNLDKRGI 140 VIRTFII HDCCH SFF NR+ANDI+GTITGV+TLVPY QWK+SH+IHHA SSNLDKRG+ Sbjct: 64 VIRTFIICHDCCHQSFFKNRRANDIVGTITGVITLVPYEQWKNSHNIHHATSSNLDKRGV 123 Query: 141 GDIWIMTVEEYLAAKPLKRLYYRIYRNPLIMFGVGPIAVFLIQYRFNARGARRKERMNTY 200 GD+W++TV EYL + +++ YR+YRNP++MFG+GPI VFL+QYRFN + ARRKER+NTY Sbjct: 124 GDLWMLTVNEYLESPIWRKIAYRLYRNPIVMFGLGPIFVFLLQYRFNVKRARRKERLNTY 183 Query: 201 LTNVSIALLYSTLIWAMGWQAFLLVQLPVVFVSGCLGIWLFYVQHQFEDSYFEHEEEWSY 260 LTN+ + LY+ LIWA+GWQAFLL+Q P+ FVSG GIWLFYVQH +EDSYFE+EEEWSY Sbjct: 184 LTNLLLVGLYAVLIWAVGWQAFLLIQGPIFFVSGLFGIWLFYVQHTYEDSYFENEEEWSY 243 Query: 261 VSAAVEGSSYYKLPKLLQWITGNIGFHHVHHLSPKVPNYNLELAHNASEPLQHATTITIG 320 V AAVEGSSYYKLPK+LQW+TGNIGFHHVHHLSP+VPNYNLE AHN + PLQ ATTIT+ Sbjct: 244 VKAAVEGSSYYKLPKVLQWLTGNIGFHHVHHLSPRVPNYNLEKAHNETPPLQKATTITLA 303 Query: 321 TSLKALHFRLWDEENKGFVGFKEIKARMKQTKPAA--EGLKVIKPGFQSE 368 S++ L F LWDE NK FV FK IK R+ + A + L+ KP + + Sbjct: 304 ASVQCLRFHLWDESNKRFVSFKGIK-RLTAARSEAIMDALQGSKPSLEGK 352 >A8VS98.1 A8VS98_9BACI Peptidase M23B Length = 340 Score = 481 bits (1237), Expect = e-134, Method: Compositional matrix adjust. Identities = 215/337 (63%), Positives = 275/337 (81%) Query: 19 QTSQLSSLKKSMAPFEKTNQRASIRQLINTLVPLVLLWMAAYFSLQVSYGLTLLFAVPAA 78 Q ++ LKK+M PFE T+++ S++Q++NTLVP LLW AY SL VSY L + F + AA Sbjct: 3 QHPKIQELKKAMKPFEVTDEQTSLKQILNTLVPYALLWTLAYLSLSVSYWLAVPFMILAA 62 Query: 79 GFVIRTFIIFHDCCHGSFFNNRKANDIIGTITGVLTLVPYRQWKHSHSIHHAGSSNLDKR 138 GF++RTFIIFHDCCH SFF +R+ANDI+GTITG LT VPY QWK++H+ HHA SSNLDKR Sbjct: 63 GFLVRTFIIFHDCCHQSFFKSRRANDIMGTITGALTHVPYEQWKNTHNAHHASSSNLDKR 122 Query: 139 GIGDIWIMTVEEYLAAKPLKRLYYRIYRNPLIMFGVGPIAVFLIQYRFNARGARRKERMN 198 G GD+WIMTV+EY A LKRL YR+YRNP++M G+GPIAVF IQYRFN + ARRKER+N Sbjct: 123 GTGDMWIMTVDEYEEAHWLKRLSYRVYRNPVMMLGIGPIAVFFIQYRFNVKQARRKERIN 182 Query: 199 TYLTNVSIALLYSTLIWAMGWQAFLLVQLPVVFVSGCLGIWLFYVQHQFEDSYFEHEEEW 258 T++TN+ IA Y+ ++ +GWQ+FLL+Q P+ +++G LGIWLFYVQHQFE++YFEH+EEW Sbjct: 183 THVTNLLIAGFYAGMMLLLGWQSFLLIQGPIAWLAGSLGIWLFYVQHQFEETYFEHDEEW 242 Query: 259 SYVSAAVEGSSYYKLPKLLQWITGNIGFHHVHHLSPKVPNYNLELAHNASEPLQHATTIT 318 SYV AA++GSS+Y+LPK+LQW+TGNIG+HHVHHLSP+VPNY L AH A+ PLQHAT IT Sbjct: 243 SYVHAAIDGSSFYELPKVLQWLTGNIGYHHVHHLSPRVPNYKLPEAHEATPPLQHATKIT 302 Query: 319 IGTSLKALHFRLWDEENKGFVGFKEIKARMKQTKPAA 355 + TSLK+L +RLWDE+NK F+ +KE K R + T A Sbjct: 303 LATSLKSLKYRLWDEDNKEFITYKEAKKRTRATAQTA 339 >A7Z1Q4.1 A7Z1Q4_BACA2 Putitative fatty acid desaturase Length = 342 Score = 465 bits (1197), Expect = e-129, Method: Compositional matrix adjust. Identities = 212/333 (63%), Positives = 258/333 (77%) Query: 22 QLSSLKKSMAPFEKTNQRASIRQLINTLVPLVLLWMAAYFSLQVSYGLTLLFAVPAAGFV 81 L +L+K + P+EK++ + SI Q+INTLVP LLW AY SL +SY LTL +V AAGF+ Sbjct: 5 NLRTLRKLVTPYEKSDLQKSICQIINTLVPFFLLWYLAYKSLSISYFLTLAISVVAAGFL 64 Query: 82 IRTFIIFHDCCHGSFFNNRKANDIIGTITGVLTLVPYRQWKHSHSIHHAGSSNLDKRGIG 141 +RTFIIFHDCCH SFF NRKAN I+GTITG+LTL P+ W H HS+HHA SSNLDKRG G Sbjct: 65 VRTFIIFHDCCHYSFFKNRKANRILGTITGILTLHPFDHWAHDHSVHHATSSNLDKRGTG 124 Query: 142 DIWIMTVEEYLAAKPLKRLYYRIYRNPLIMFGVGPIAVFLIQYRFNARGARRKERMNTYL 201 DIW++TVEEY A ++ YR+YRNP +MF +GPI VF I RFN +GA+RKERMNTY+ Sbjct: 125 DIWVLTVEEYKEASTKTKIMYRLYRNPFVMFIIGPIYVFGITNRFNRKGAKRKERMNTYV 184 Query: 202 TNVSIALLYSTLIWAMGWQAFLLVQLPVVFVSGCLGIWLFYVQHQFEDSYFEHEEEWSYV 261 TN+ I L + L WA+GWQ FLLVQ P+ +SG LGIW+FY+QH FEDSYFE +E W YV Sbjct: 185 TNLGIVALAALLCWAIGWQTFLLVQTPIFMISGSLGIWMFYIQHTFEDSYFEEDEHWEYV 244 Query: 262 SAAVEGSSYYKLPKLLQWITGNIGFHHVHHLSPKVPNYNLELAHNASEPLQHATTITIGT 321 AAVEGSS+YKLPK++QW+TGNIGFHHVHHLSP+VPNY LE+ HN +EPLQ+ TIT+ T Sbjct: 245 KAAVEGSSFYKLPKVMQWLTGNIGFHHVHHLSPRVPNYKLEIVHNNTEPLQNVPTITLAT 304 Query: 322 SLKALHFRLWDEENKGFVGFKEIKARMKQTKPA 354 S+K+L FRLWDEE K FVGF +K K A Sbjct: 305 SIKSLKFRLWDEEGKKFVGFTHLKKTSKSQVSA 337 >Q65N39.1 Q65N39_BACLD Putative uncharacterized protein Length = 342 Score = 460 bits (1184), Expect = e-128, Method: Compositional matrix adjust. Identities = 211/333 (63%), Positives = 257/333 (77%) Query: 22 QLSSLKKSMAPFEKTNQRASIRQLINTLVPLVLLWMAAYFSLQVSYGLTLLFAVPAAGFV 81 L +L+K + P+EK++ + SI Q+INTL+P LLW AY SL +SY LTL ++ AAGF+ Sbjct: 5 NLKTLRKLVTPYEKSDLQKSIYQIINTLIPFFLLWCLAYKSLSISYFLTLAISIVAAGFL 64 Query: 82 IRTFIIFHDCCHGSFFNNRKANDIIGTITGVLTLVPYRQWKHSHSIHHAGSSNLDKRGIG 141 +RTFIIFHDCCH SFF N+KAN I+GTITG+LTL P+ W HSIHHA SSNLDKRG G Sbjct: 65 VRTFIIFHDCCHYSFFKNKKANRILGTITGILTLHPFDHWGRDHSIHHATSSNLDKRGTG 124 Query: 142 DIWIMTVEEYLAAKPLKRLYYRIYRNPLIMFGVGPIAVFLIQYRFNARGARRKERMNTYL 201 DIW++TVEEY A ++ YR+YRNP +MF +GPI VF I RFN +GA+RKERMNTY+ Sbjct: 125 DIWLLTVEEYKEASTKTKIMYRLYRNPFVMFMIGPIYVFGITNRFNRKGAKRKERMNTYV 184 Query: 202 TNVSIALLYSTLIWAMGWQAFLLVQLPVVFVSGCLGIWLFYVQHQFEDSYFEHEEEWSYV 261 TN+ IA L + L WA+GWQ FLLVQ P+ +SG LGIW+FY+QH FEDSYFE +E W YV Sbjct: 185 TNLGIAALTALLCWAIGWQNFLLVQAPIFMISGSLGIWMFYIQHTFEDSYFEEDEHWEYV 244 Query: 262 SAAVEGSSYYKLPKLLQWITGNIGFHHVHHLSPKVPNYNLELAHNASEPLQHATTITIGT 321 AAVEGSS+YKLPK++QW+TGNIGFHHVHHLSP+VPNY LE HN EPLQ+ TIT+ T Sbjct: 245 KAAVEGSSFYKLPKVMQWLTGNIGFHHVHHLSPRVPNYKLEEVHNNIEPLQNVPTITLAT 304 Query: 322 SLKALHFRLWDEENKGFVGFKEIKARMKQTKPA 354 SLK+L FRLWDEE+K FVGF +K K A Sbjct: 305 SLKSLKFRLWDEESKKFVGFSHLKKASKSQVSA 337 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.137 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,216,613,557 Number of extensions: 91209934 Number of successful extensions: 291985 Number of sequences better than 1.0e-05: 658 Number of HSP's gapped: 291452 Number of HSP's successfully gapped: 674 Length of query: 368 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 231 Effective length of database: 1,770,017,148 Effective search space: 408873961188 Effective search space used: 408873961188 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)