BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0040.p (385 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9E4.1 B3K9E4_9BACI Histidine kinase dimerisation and phosphoa... 544 e-153 C0Z4P3.1 C0Z4P3_BREBN Two-component sensor histidine kinase EC=2... 534 e-150 Q2BDU1.1 Q2BDU1_9BACI Putative uncharacterized protein 441 e-122 Q8EMJ1.1 Q8EMJ1_OCEIH Two-component sensor histidine kinase 409 e-112 A7ZAI9.1 A7ZAI9_BACA2 YocF 391 e-107 >B3K9E4.1 B3K9E4_9BACI Histidine kinase dimerisation and phosphoacceptor region Length = 377 Score = 544 bits (1402), Expect = e-153, Method: Compositional matrix adjust. Identities = 257/375 (68%), Positives = 315/375 (84%), Gaps = 3/375 (0%) Query: 4 MQKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFK 63 MQKW+ IFHKSTGLSPYVWVVFYILPFYFIFRS S Q V G++M+ +FFA YVLSF Sbjct: 1 MQKWYQIFHKSTGLSPYVWVVFYILPFYFIFRSTSTYQ--IVIGVLMIVMFFAFYVLSFL 58 Query: 64 SKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAA 123 SKGW+VYFWT++QIL+S+TM+++FGY+YF+LF+AFFIGNI+ +AGF TLYSIHL+TTI Sbjct: 59 SKGWLVYFWTSLQILISVTMSVVFGYIYFSLFLAFFIGNIKNKAGFITLYSIHLVTTIIT 118 Query: 124 INYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIME 183 +NY ++T N FI+QLPFVLVS++ V+LLP T YNRN D+LQGQLEDANKRISELV +E Sbjct: 119 VNYGLLTRNPFFITQLPFVLVSVVGVILLPVTNYNRNKNDQLQGQLEDANKRISELVKLE 178 Query: 184 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMV 243 ERQRIARDLHDTLGQKLSLIGLKSDLAGKL+ ++ +A EINDVRQTAR+ALKEVREMV Sbjct: 179 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLMKQDMERAQAEINDVRQTARTALKEVREMV 238 Query: 244 TQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHS 303 T+MRG RL DE+ RIRQ+L+AA+IE EG+ + N SLM ENV+SMCLKEAV N+VKHS Sbjct: 239 TRMRGTRLADEIFRIRQILKAAQIELTFEGTLEPENLSLMNENVISMCLKEAVNNIVKHS 298 Query: 304 GATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGSMDVLQDKG 363 AT C + IE + TDL+ +VKDNGTG ++ ++G+GL+GMRERLEFVNGSM+ +G Sbjct: 299 NATACSIVIESTETDLIARVKDNGTGFV-TDAKNRGNGLRGMRERLEFVNGSMEFASGEG 357 Query: 364 TTLIIRVPNVFKQPE 378 TTL++RVPNV + P+ Sbjct: 358 TTLVLRVPNVLRMPD 372 >C0Z4P3.1 C0Z4P3_BREBN Two-component sensor histidine kinase EC=2.7.3.- Length = 396 Score = 534 bits (1375), Expect = e-150, Method: Compositional matrix adjust. Identities = 254/375 (67%), Positives = 307/375 (81%), Gaps = 4/375 (1%) Query: 5 QKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFKS 64 QKW+HI K+TGLSPYVWVVFYILPFYFIFRS+S + V GI+M+ +FF CYV SF S Sbjct: 20 QKWYHILQKNTGLSPYVWVVFYILPFYFIFRSSSTYEA--VIGIIMIILFFICYVFSFLS 77 Query: 65 KGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAAI 124 KGW++YFWT VQI++SI MTL+FGY+YF+LF+AFFIGNI+ + GF TLY++HL++T ++ Sbjct: 78 KGWLIYFWTGVQIIISIAMTLMFGYVYFSLFLAFFIGNIENKIGFVTLYTVHLISTAISV 137 Query: 125 NYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIMEE 184 N T N I QLPFVLVSM+AV+LLP TTYN N ++KLQG+LE ANKRISELV +EE Sbjct: 138 NIGFFTKNAQLIGQLPFVLVSMVAVILLPVTTYNWNKREKLQGELEHANKRISELVKLEE 197 Query: 185 RQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMVT 244 RQRIARDLHDTLGQKLSLIGLKSDLA KLI+KNPA A E+ ++RQTAR ALKEVREMVT Sbjct: 198 RQRIARDLHDTLGQKLSLIGLKSDLANKLINKNPALARTELEEIRQTARIALKEVREMVT 257 Query: 245 QMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHSG 304 QMRG RLEDE+ RI+Q+ +AA+IEF LEG L NTSLMTENVL MCLKEAV N+VKHS Sbjct: 258 QMRGTRLEDEMFRIKQIFKAADIEFVLEGDTNLTNTSLMTENVLCMCLKEAVNNIVKHSN 317 Query: 305 ATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGSMDVLQD-KG 363 AT + IEP+RTDL++ V DNG G+ +Y +G+GL+GM+ERLEFVNGSM++ G Sbjct: 318 ATASSIVIEPTRTDLVVSVTDNGIGMAEELVY-RGNGLRGMKERLEFVNGSMEIQSSVSG 376 Query: 364 TTLIIRVPNVFKQPE 378 T L+IRVPN+FKQPE Sbjct: 377 TQLVIRVPNIFKQPE 391 >Q2BDU1.1 Q2BDU1_9BACI Putative uncharacterized protein Length = 378 Score = 441 bits (1135), Expect = e-122, Method: Compositional matrix adjust. Identities = 216/380 (56%), Positives = 282/380 (74%), Gaps = 2/380 (0%) Query: 4 MQKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFK 63 M+K +F+ STG+SPY+W + ILPFYFIF+S+S + V GI++ +F+ + L+F Sbjct: 1 MKKKLLVFNSSTGISPYIWAILTILPFYFIFQSSSTAE--IVAGIILTVIFYLFFRLAFL 58 Query: 64 SKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAA 123 S+GW VY W V I +S MT LFGY+YF+ FIA+FIGNI +A F LY +HL AA Sbjct: 59 SRGWTVYIWAIVLIGISTAMTTLFGYVYFSFFIAYFIGNITGKAAFAILYILHLTAASAA 118 Query: 124 INYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIME 183 INY+ + + +F+ Q+PF+++S ++V+LLP + NRN + KL+ QLEDANKRISELV ME Sbjct: 119 INYQFVLQDPLFVKQIPFIIISWLSVILLPFSIKNRNMRGKLESQLEDANKRISELVKME 178 Query: 184 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMV 243 ERQRIARDLHDTLGQKLSLIGLKSDLAGKL++K+P QA E+ DV+QTAR+AL EVR+MV Sbjct: 179 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLVYKDPEQARSELKDVQQTARTALNEVRKMV 238 Query: 244 TQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHS 303 +QMRGIRL++E++ I Q+L AA I F L+ +LVN SL EN+LSMCLKEAV NVVKHS Sbjct: 239 SQMRGIRLKEEVVLIEQILGAAGISFVLKQERQLVNVSLFVENILSMCLKEAVNNVVKHS 298 Query: 304 GATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNGSMDVLQDKG 363 AT V+I + + + V DNG GI+ KG GL G+RER EFVNG+M+V QD G Sbjct: 299 NATEVIVEIRQADDETAVTVSDNGVGIDPDRDIGKGSGLLGIRERAEFVNGTMEVYQDGG 358 Query: 364 TTLIIRVPNVFKQPELKEVK 383 TTLI+RVPNV K + +E++ Sbjct: 359 TTLIVRVPNVVKIRQKEELQ 378 >Q8EMJ1.1 Q8EMJ1_OCEIH Two-component sensor histidine kinase Length = 380 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 202/377 (53%), Positives = 277/377 (73%), Gaps = 4/377 (1%) Query: 4 MQKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFK 63 MQ W+H+F K+TGLS + W+ F +LPFYFI RS+S+ V G++M+ +FFA Y L+F Sbjct: 1 MQHWYHLFPKNTGLSLFAWIAFCLLPFYFILRSSSLIDI--VVGVIMIVIFFATYRLAFI 58 Query: 64 SKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAA 123 KGW VY ++I +S+ +TL FGY+YFALF+AFFIGNIQ +AGF +LY IHL+TT+ A Sbjct: 59 KKGWTVYVSVGIEIFISVGVTLYFGYVYFALFLAFFIGNIQNKAGFISLYIIHLVTTLTA 118 Query: 124 INYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIME 183 +++ +F +Q PF+++ +I V+LLP T YNRN ++KL+ QLEDAN++I++L+++E Sbjct: 119 VSFGFFIQEEMFYNQWPFIVICVIGVILLPFTMYNRNKREKLENQLEDANQKIAQLIVLE 178 Query: 184 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMV 243 ER+RIARDLHDTLGQKLSL+GLKS+LAGKL+ P A E+ D+ QTAR+ALKEVRE+V Sbjct: 179 ERERIARDLHDTLGQKLSLMGLKSELAGKLVDAKPESAKKELADIHQTARTALKEVRELV 238 Query: 244 TQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHS 303 M+G +L+DELIR+RQLLE A+I ++E + K L+ ENVLSMCLKEAVTNVVKHS Sbjct: 239 AGMKGTKLKDELIRVRQLLEVAQITCEIEDNIKHSEVPLLIENVLSMCLKEAVTNVVKHS 298 Query: 304 GATLCQVQIEPSRTDLLIKVKDNGTGIEGSNLYDKGHGLQGMRERLEFVNG--SMDVLQD 361 AT C++ ++ + +I++ DNG GI+ KG+G+ GM+ERL FVNG S D Sbjct: 299 EATECKIVVKQTPDKWMIRIIDNGIGIQDKVHTFKGNGILGMKERLGFVNGNLSFDETNK 358 Query: 362 KGTTLIIRVPNVFKQPE 378 T L I+VP V +Q E Sbjct: 359 SKTILSIQVPRVIQQTE 375 >A7ZAI9.1 A7ZAI9_BACA2 YocF Length = 378 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 187/376 (49%), Positives = 271/376 (72%), Gaps = 4/376 (1%) Query: 4 MQKWHHIFHKSTGLSPYVWVVFYILPFYFIFRSASVNQNQWVYGIVMVAVFFACYVLSFK 63 M+K+ F K G+SPY+W++F+ILPFYFIF+S+S V GI+ VFF Y +F Sbjct: 1 MKKYFK-FQKLNGISPYIWMIFFILPFYFIFKSSSTFV--IVAGIIFTLVFFGAYRFAFV 57 Query: 64 SKGWVVYFWTTVQILVSITMTLLFGYMYFALFIAFFIGNIQKRAGFFTLYSIHLLTTIAA 123 +KGW +Y W + I +S +LF Y+YFA FIA+FIG+I+ + F+ LY IH+++ A Sbjct: 58 AKGWPLYLWALLLIGISTGSAMLFSYIYFAFFIAYFIGHIRDKVPFYILYYIHIISAAVA 117 Query: 124 INYEMITGNRVFISQLPFVLVSMIAVVLLPATTYNRNNQDKLQGQLEDANKRISELVIME 183 +N+ ++ F++Q+PF+++++I+ +LLP + +R +++L+ +LE AN+RI++LV +E Sbjct: 118 VNFSLVLKKEWFLTQIPFIVITLISAILLPLSIRSRKARERLEEKLEYANERIADLVKLE 177 Query: 184 ERQRIARDLHDTLGQKLSLIGLKSDLAGKLIHKNPAQAAVEINDVRQTARSALKEVREMV 243 ERQRIARDLHDTLGQKLSLIGLKSDLA KLI+K+P QA E+ V+QTAR++L EVR++V Sbjct: 178 ERQRIARDLHDTLGQKLSLIGLKSDLARKLIYKDPEQARTELKSVQQTARTSLNEVRKIV 237 Query: 244 TQMRGIRLEDELIRIRQLLEAAEIEFQLEGSPKLVNTSLMTENVLSMCLKEAVTNVVKHS 303 + M+GIRL+DEL +RQ+LEAA IEF E + SL+ EN++SMC+KEAVTNVVKHS Sbjct: 238 SSMKGIRLKDELGNVRQILEAAGIEFVYEEKEAPKHISLLNENIVSMCIKEAVTNVVKHS 297 Query: 304 GATLCQVQIEPSRTDLLIKVKDNGT-GIEGSNLYDKGHGLQGMRERLEFVNGSMDVLQDK 362 GA +C++ I+ +++I V+D+G+ + + KGHGL GMRERLEF NGS+ + Sbjct: 298 GAKVCRITIQQLWKEVVIMVEDDGSFQCKEDRFFSKGHGLLGMRERLEFANGSLAIDTAA 357 Query: 363 GTTLIIRVPNVFKQPE 378 GT L +R+PN K E Sbjct: 358 GTKLTMRIPNDSKSKE 373 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.138 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,082,847,129 Number of extensions: 81645148 Number of successful extensions: 360161 Number of sequences better than 1.0e-05: 3513 Number of HSP's gapped: 355953 Number of HSP's successfully gapped: 3514 Length of query: 385 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 248 Effective length of database: 1,770,017,148 Effective search space: 438964252704 Effective search space used: 438964252704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)