BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0036.p (214 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCC6.1 B1DCC6_9BACL Peptidase C60 sortase A and B 142 2e-32 C0ZCK3.1 C0ZCK3_BREBN Putative uncharacterized protein 113 1e-23 B4AJQ6.1 B4AJQ6_BACPU Putative C60 family sortase A 104 6e-21 A8FIT9.1 A8FIT9_BACP2 Possible C60 family sortase A 104 6e-21 A6CP91.1 A6CP91_9BACI Putative uncharacterized protein 103 8e-21 >B1DCC6.1 B1DCC6_9BACL Peptidase C60 sortase A and B Length = 212 Score = 142 bits (358), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 73/197 (37%), Positives = 119/197 (60%), Gaps = 7/197 (3%) Query: 18 TGCAGSGQQMDNRPDSSTPRPL---QTMPEHLNTAEKGLNENHAVIDEPKP-FMPDLLTI 73 TGC + ++ ++ T P+ ++P H + H ID P +P+ + I Sbjct: 17 TGCTSEPSKQHSQTNNETALPVPSPTSVPLHAAELKTPAKPKH--IDAKTPVIVPNRIWI 74 Query: 74 PAISVKAGIQPVSLLENGQMDVPDNTEITGILFPGVLPGGKGNVLXXXXXXXXXXXXXXX 133 P++ +KA ++PV LL+NGQ+DVP +++I G+ GVLPG +GN + Sbjct: 75 PSVGIKAPVEPVGLLDNGQLDVPASSDIAGLFIDGVLPGEEGNAIIAGHVDNYTGPAIFY 134 Query: 134 XXXXXXXQGDKVIVSSKTGRQLTYIVESVEAFTPAEAPMERIFGETGERRMNLITCTGRY 193 QGD V++S +G+ L Y V++VE+++ AEAP+++IFG+T +R+N+ITCTG+Y Sbjct: 135 PLKKLK-QGDPVVLSDSSGKYLVYSVDAVESYSTAEAPLDKIFGDTNGKRLNMITCTGKY 193 Query: 194 SRKKKEHEKRLIIFTKL 210 +R KKEHEKRL+++T L Sbjct: 194 NRLKKEHEKRLVVYTHL 210 >C0ZCK3.1 C0ZCK3_BREBN Putative uncharacterized protein Length = 229 Score = 113 bits (282), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 58/148 (39%), Positives = 86/148 (58%), Gaps = 1/148 (0%) Query: 63 PKPFMPDLLTIPAISVKAGIQPVSLLENGQMDVPDNTEITGILFPGVLPGGKGNVLXXXX 122 P MP LL IP ++++A ++PV +L NGQMDVP + GIL P PG KGN + Sbjct: 68 PDGIMPTLLHIPKLNLQARVEPVGVLPNGQMDVPKAFDRVGILSPWTKPGMKGNAVIAGH 127 Query: 123 XXXXXXXXXXXXXXXXXXQGDKVIVSSKTGRQLTYIVESVEAFTPAEAPMERIFGETGER 182 GDK+ V G+ LT+ V VE+F AEAP+++IFG Sbjct: 128 FDHYTGPAVFYHLKKLKP-GDKITVREAKGKTLTFQVRRVESFQTAEAPIDQIFGNAESS 186 Query: 183 RMNLITCTGRYSRKKKEHEKRLIIFTKL 210 +NLITC+G++++K +EH +RL++F++L Sbjct: 187 HLNLITCSGKFNKKTQEHARRLVVFSEL 214 >B4AJQ6.1 B4AJQ6_BACPU Putative C60 family sortase A Length = 204 Score = 104 bits (259), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 72/212 (33%), Positives = 108/212 (50%), Gaps = 13/212 (6%) Query: 1 MKKRILAFITAAIVLSVTGCAGSGQQMDNRPDSSTPRPLQTMPEHL--NTAEKGLNENHA 58 MK+ + + ++L +TGCA + MD DSS+ + + PE + N A K + Sbjct: 1 MKRWLFGLL---MLLVITGCAS--KSMD---DSSSAKTTSS-PEKMTTNQAAKQSHSKAM 51 Query: 59 VIDEPKPFMPDLLTIPAISVKAGIQPVSLLENGQMDVPDNTEITGILFPGVLPGGKGNVL 118 + DEP +P + IPAI VKA I+ +L ++G M VP NT+ T G PG KGN + Sbjct: 52 LKDEPSGIVPAEIAIPAIDVKAKIEKTTLSKDGSMGVPKNTDNTAWFKDGPKPGDKGNAV 111 Query: 119 XXXXXXXXXXXXXXXXXXXXXXQGDKVIVSSKTGRQLTYIVESVEAFTPAEAPMERIFGE 178 +G+KVIV+S + ++ T+ V V+++ E P FG Sbjct: 112 -MNGHVDNKWGPSVFYRLKELKKGNKVIVTSISDKKRTFEVVKVQSYPREEKP-NAFFGY 169 Query: 179 TGERRMNLITCTGRYSRKKKEHEKRLIIFTKL 210 R +NLITCTG + HEKRL++FT+L Sbjct: 170 AFTRNLNLITCTGTFDHAAGTHEKRLVVFTQL 201 >A8FIT9.1 A8FIT9_BACP2 Possible C60 family sortase A Length = 204 Score = 104 bits (259), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 70/212 (33%), Positives = 107/212 (50%), Gaps = 13/212 (6%) Query: 1 MKKRILAFITAAIVLSVTGCAGSGQQMDNRPDSSTPRPLQTMPEHLNTAEKGLNENHAVI 60 MK+ + + ++L +TGCA + MD DSS+ + + PE + T + + I Sbjct: 1 MKRWLFGLL---MLLIITGCAS--KSMD---DSSSAKTTSS-PEKITTNQAAKQSHSKAI 51 Query: 61 --DEPKPFMPDLLTIPAISVKAGIQPVSLLENGQMDVPDNTEITGILFPGVLPGGKGNVL 118 DEP +P + IPAI VKA ++ +L ++G M VP T+ T G PG KGN + Sbjct: 52 LKDEPSGIVPAEIAIPAIGVKAKVEKTTLSKDGSMGVPKKTDNTAWFKDGPKPGDKGNAV 111 Query: 119 XXXXXXXXXXXXXXXXXXXXXXQGDKVIVSSKTGRQLTYIVESVEAFTPAEAPMERIFGE 178 +G+KVIV+S +G++ T+ V V+++ E P FG Sbjct: 112 -MNGHVDNKWGPSVFYRLKELKKGNKVIVTSSSGKKRTFEVIKVQSYPREEKP-NAFFGY 169 Query: 179 TGERRMNLITCTGRYSRKKKEHEKRLIIFTKL 210 R +NLITCTG + HEKRL++FT+L Sbjct: 170 AFTRNLNLITCTGTFDHAAGTHEKRLVVFTQL 201 >A6CP91.1 A6CP91_9BACI Putative uncharacterized protein Length = 230 Score = 103 bits (258), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 71/212 (33%), Positives = 116/212 (54%), Gaps = 9/212 (4%) Query: 1 MKKRILAFITAAIVLSVTGCAGSGQQMDNRPDSSTPRPLQTMPEHLNTAEKGLNENHAVI 60 +KK IL + +++S G S ++ + TP P +P ++AE + A Sbjct: 24 LKKTILLYWI--LLISTIGVNTSAAPRESDV-TVTPSPTVEVP---SSAETNQADKPAAG 77 Query: 61 DEPKPF--MPDLLTIPAISVKAGIQPVSLLENGQMDVPDNTEITGILFPGVLPGGKGNVL 118 D + +P+ +TIP+I ++A + V +LENG+M+VPDN G PGV PGG+GN + Sbjct: 78 DYLQTIEVIPEKITIPSIGMEAQVAGVGILENGEMEVPDNINQAGWFKPGVKPGGQGNAV 137 Query: 119 XXXXXXXXXXXXXXXXXXXXXXQGDKVIVSSKTGRQLTYIVESVEAFTPAEAPMERIFGE 178 +GDKV + K G+ LT+ V VE++ +AP+ERIFG Sbjct: 138 LAGHLDGKGKPGAFYHIGKLQ-KGDKVEILGKDGQLLTFQVVGVESYYTDDAPLERIFGY 196 Query: 179 TGERRMNLITCTGRYSRKKKEHEKRLIIFTKL 210 + R+NLITC+G + ++ E+E+RL+++T+L Sbjct: 197 HSDSRLNLITCSGDFDEEEHEYEERLVVYTEL 228 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,182,390,317 Number of extensions: 48079509 Number of successful extensions: 106067 Number of sequences better than 1.0e-05: 53 Number of HSP's gapped: 106037 Number of HSP's successfully gapped: 53 Length of query: 214 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 84 Effective length of database: 1,835,964,253 Effective search space: 154220997252 Effective search space used: 154220997252 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)