BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0017.p (218 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A6CIN6.1 A6CIN6_9BACI Thiamine-phosphate pyrophosphorylase EC=2.... 215 2e-54 C2W3J4.1 C2W3J4_BACCE Thiamine-phosphate pyrophosphorylase 208 2e-52 C3AYS4.1 C3AYS4_BACMY Thiamine-phosphate pyrophosphorylase 208 3e-52 B4BKC2.1 B4BKC2_9BACI Thiamine-phosphate pyrophosphorylase EC=2.... 207 7e-52 C3BFH2.1 C3BFH2_9BACI Thiamine-phosphate pyrophosphorylase 206 1e-51 >A6CIN6.1 A6CIN6_9BACI Thiamine-phosphate pyrophosphorylase EC=2.5.1.3 Length = 222 Score = 215 bits (548), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 102/181 (56%), Positives = 133/181 (73%) Query: 6 SEHVRRLLKLYFIMGSVNCLKPPLETLVEAMDGGITLFQYREKGSAAFTGQAYIELAQVL 65 S+ ++ LLK+YFIMGS NCL+ P E L A+ G ITLFQ+REKG+ G+ ++LA+ L Sbjct: 5 SQQIKELLKVYFIMGSANCLQHPEEVLRGAIKGDITLFQFREKGNGCLQGEDKLQLAKRL 64 Query: 66 QQKCREHKIPFIVNXXXXXXXXXXXXGVHVGQKDAPARSIRERLGAHKIIGVSAHNLKEA 125 Q C+E++IPFIVN GVH+GQ+D PA +R ++G KI+GVSAH L+EA Sbjct: 65 QSICQENQIPFIVNDDIELAVELDADGVHIGQEDEPAEDVRRKIGNKKILGVSAHTLEEA 124 Query: 126 QQAIADGADYLGVGPVYPTSSKDDAEAVQGTGVIEELRRSGLTIPLVGIGGITAANAQPV 185 + AIA GADYLG+GP+YPT +K+DA+AVQG I+ELR SG+ IPLVGIGGITA NA P+ Sbjct: 125 EHAIAAGADYLGIGPIYPTLTKEDAKAVQGLTFIQELRESGIVIPLVGIGGITAENAAPI 184 Query: 186 I 186 I Sbjct: 185 I 185 >C2W3J4.1 C2W3J4_BACCE Thiamine-phosphate pyrophosphorylase Length = 218 Score = 208 bits (530), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 98/187 (52%), Positives = 134/187 (71%), Gaps = 1/187 (0%) Query: 1 MQRMDSEHVRRLLKLYFIMGSVNCLKPPLETLVEAMDGGITLFQYREKGSAAFTGQAYIE 60 M R+++E + +LL++YFIMGS NC + PL+ + EA+DGGITLFQ+REKG A T + + Sbjct: 4 MARIETEQMSKLLQVYFIMGSNNCKRDPLQVMKEALDGGITLFQFREKGEGALTREERVR 63 Query: 61 LAQVLQQKCREHKIPFIVNXXXXXXXXXXXXGVHVGQKDAPARSIRERLGAHKIIGVSAH 120 A+ LQ C+E+ +PFIVN GVHVGQ D S+RE++G KI+GVSAH Sbjct: 64 FAKQLQALCKEYDVPFIVNDDVELAIELDADGVHVGQDDEGITSVREKMG-DKIVGVSAH 122 Query: 121 NLKEAQQAIADGADYLGVGPVYPTSSKDDAEAVQGTGVIEELRRSGLTIPLVGIGGITAA 180 ++EA+ AIA+GADYLGVGP++PTS+K D +AVQGT + R +G+T+P+VGIGGIT Sbjct: 123 TIEEARFAIANGADYLGVGPIFPTSTKKDTKAVQGTKGLRHFREAGITVPIVGIGGITVE 182 Query: 181 NAQPVIQ 187 NA VI+ Sbjct: 183 NAASVIE 189 >C3AYS4.1 C3AYS4_BACMY Thiamine-phosphate pyrophosphorylase Length = 224 Score = 208 bits (529), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 98/187 (52%), Positives = 132/187 (70%), Gaps = 1/187 (0%) Query: 1 MQRMDSEHVRRLLKLYFIMGSVNCLKPPLETLVEAMDGGITLFQYREKGSAAFTGQAYIE 60 M R++ + + +LL++YFIMGS NC K PL + EA+DGG+TLFQ+REKG A TG+ + Sbjct: 4 MARINQQQMSKLLQVYFIMGSNNCQKDPLRVMKEALDGGVTLFQFREKGEGALTGKERVH 63 Query: 61 LAQVLQQKCREHKIPFIVNXXXXXXXXXXXXGVHVGQKDAPARSIRERLGAHKIIGVSAH 120 A+ LQ C+E+ +PFIVN G+HVGQ D S+RE++G KI+GVSAH Sbjct: 64 FAKQLQALCKEYNVPFIVNDDVDLAIELDADGIHVGQDDEGITSVREKMG-DKIVGVSAH 122 Query: 121 NLKEAQQAIADGADYLGVGPVYPTSSKDDAEAVQGTGVIEELRRSGLTIPLVGIGGITAA 180 ++EA+ AIA+GADYLGVGP++PTS+K D +AVQGT + R G+TIP+VGIGGIT Sbjct: 123 TIEEARFAIANGADYLGVGPIFPTSTKKDTKAVQGTKGLRYFREEGITIPIVGIGGITVE 182 Query: 181 NAQPVIQ 187 NA VI+ Sbjct: 183 NAASVIE 189 >B4BKC2.1 B4BKC2_9BACI Thiamine-phosphate pyrophosphorylase EC=2.5.1.3 Length = 221 Score = 207 bits (526), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 101/187 (54%), Positives = 131/187 (70%), Gaps = 1/187 (0%) Query: 1 MQRMDSEHVRRLLKLYFIMGSVNCLKPPLETLVEAMDGGITLFQYREKGSAAFTGQAYIE 60 M R+ SE +++ L +YFIMGS N +P + L EA+DGG+TLFQ+REKGSAA G+ Sbjct: 1 MARITSEEMKKRLAVYFIMGSQNSERPAEDVLKEALDGGVTLFQFREKGSAALEGEEKEA 60 Query: 61 LAQVLQQKCREHKIPFIVNXXXXXXXXXXXXGVHVGQKDAPARSIRERLGAHKIIGVSAH 120 LA+ LQ+ CR + +PFIVN GVHVGQ D AR +RE++G KI+GVSAH Sbjct: 61 LARQLQRLCRTYGVPFIVNDDVELAIAIDADGVHVGQDDEDARRVREKIG-DKILGVSAH 119 Query: 121 NLKEAQQAIADGADYLGVGPVYPTSSKDDAEAVQGTGVIEELRRSGLTIPLVGIGGITAA 180 N++EA+ AI GADY+GVGP+YPT SKDDA QG G++ LR G+TIP+V IGGITA Sbjct: 120 NVEEARAAIEAGADYIGVGPIYPTRSKDDANEAQGPGILRHLREQGITIPIVAIGGITAD 179 Query: 181 NAQPVIQ 187 N + VI+ Sbjct: 180 NTRAVIE 186 >C3BFH2.1 C3BFH2_9BACI Thiamine-phosphate pyrophosphorylase Length = 224 Score = 206 bits (525), Expect = 1e-51, Method: Compositional matrix adjust. Identities = 96/187 (51%), Positives = 132/187 (70%), Gaps = 1/187 (0%) Query: 1 MQRMDSEHVRRLLKLYFIMGSVNCLKPPLETLVEAMDGGITLFQYREKGSAAFTGQAYIE 60 M R++ + + +LL++YFIMGS NC K PL + EA+DGG+TLFQ+REKG A TG+ + Sbjct: 4 MARINQQQMSKLLQVYFIMGSNNCQKDPLRVMKEALDGGVTLFQFREKGEGALTGKERVH 63 Query: 61 LAQVLQQKCREHKIPFIVNXXXXXXXXXXXXGVHVGQKDAPARSIRERLGAHKIIGVSAH 120 A+ LQ C+E+ +PFIVN G+HVGQ D +RE++G KI+GVSAH Sbjct: 64 FAKQLQALCKEYNVPFIVNDDVDLAIELDADGIHVGQDDEGITFVREKMG-DKIVGVSAH 122 Query: 121 NLKEAQQAIADGADYLGVGPVYPTSSKDDAEAVQGTGVIEELRRSGLTIPLVGIGGITAA 180 ++EA+ AIA+GADYLGVGP++PTS+K D +AVQGT + R +G+T+P+VGIGGIT Sbjct: 123 TIEEARFAIANGADYLGVGPIFPTSTKKDTKAVQGTKGLRHFREAGITVPIVGIGGITVK 182 Query: 181 NAQPVIQ 187 NA VI+ Sbjct: 183 NAASVIE 189 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,031,046,174 Number of extensions: 37390799 Number of successful extensions: 98500 Number of sequences better than 1.0e-05: 1374 Number of HSP's gapped: 97097 Number of HSP's successfully gapped: 1375 Length of query: 218 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 88 Effective length of database: 1,835,964,253 Effective search space: 161564854264 Effective search space used: 161564854264 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)