BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0011.p (217 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DKE0.1 B1DKE0_9BACL Transcriptional regulator, TetR family 97 8e-19 B1DDS4.1 B1DDS4_9BACL Transcriptional regulator, TetR family 95 5e-18 B3K446.1 B3K446_9BACI Regulatory protein TetR 94 9e-18 Q1AX63.1 Q1AX63_RUBXD Transcriptional regulator, TetR family 94 1e-17 C2K3D5.1 C2K3D5_STAAU TetR family transcriptional regulator 77 9e-13 >B1DKE0.1 B1DKE0_9BACL Transcriptional regulator, TetR family Length = 201 Score = 97.4 bits (241), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 60/183 (32%), Positives = 105/183 (57%), Gaps = 4/183 (2%) Query: 31 QRILEAAEALFEENGLEPVSMYGIAKRAGIGQGSLYRRFTDKGEICSALLSTSSERFLSR 90 Q ILE A ALF E G+E VSM IA+ AG+GQG++YR +T+KGE+C L+ S + R Sbjct: 15 QIILEKAAALFAEYGVEHVSMRQIAREAGVGQGTIYRSYTNKGELCWDLIGESCIQNYDR 74 Query: 91 LEQELQATTGASNSLED-LQSSIEQVVDFIDQHAELLQMIKSEFTG-KKQLTQFEHPIFR 148 ++ L T S+ ++D L++ + ++ ++ H+ +L I++ G ++Q F + Sbjct: 75 IQSFLSDHT--SSPIKDRLETVLLYHLESLETHSPVLAAIQANSGGDEQQNVPFYSEHYE 132 Query: 149 RLNEVMTQRLRRAADDGLLIAVDPQFAATALIAVLSPDLYLYQQKLHNSTKEEISRGVLA 208 ++ V+ Q L A + +D F A A++A L PD+Y++Q++ + EEI + Sbjct: 133 SIHSVIVQLLNETATKNENLVLDAVFMADAIMATLRPDVYIFQRQHRGYSPEEIRNKLFH 192 Query: 209 LFV 211 +F+ Sbjct: 193 VFI 195 >B1DDS4.1 B1DDS4_9BACL Transcriptional regulator, TetR family Length = 200 Score = 94.7 bits (234), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 56/175 (32%), Positives = 100/175 (57%), Gaps = 4/175 (2%) Query: 31 QRILEAAEALFEENGLEPVSMYGIAKRAGIGQGSLYRRFTDKGEICSALLSTSSERFLSR 90 + IL+ A +LF E+G+E VSM+ IAK IGQG+LYRR+ +K ++C L++ + E + Sbjct: 13 KMILQTARSLFAEHGVEAVSMHQIAKSIQIGQGTLYRRYANKSDLCMDLMNDTFEDLMEE 72 Query: 91 LEQELQATTGASNSLED-LQSSIEQVVDFIDQHAELLQMIKSEFTGK-KQLTQFEHPIFR 148 + + L+ A + D L + + +++ F + E L +IK T K + +E P +R Sbjct: 73 IRRLLEPL--ADKPVRDRLTAVLRRLIVFSNDQLEWLNVIKVSKTCKPDDMDFYESPNYR 130 Query: 149 RLNEVMTQRLRRAADDGLLIAVDPQFAATALIAVLSPDLYLYQQKLHNSTKEEIS 203 +LN + L+ A +IA+ P+F A LI+ ++P+ +Y K+ + E+I+ Sbjct: 131 KLNNLFLSLLQEAQSKDEIIALIPEFTAHMLISAMAPESLIYYSKVLGYSIEQIA 185 >B3K446.1 B3K446_9BACI Regulatory protein TetR Length = 196 Score = 94.0 bits (232), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 61/186 (32%), Positives = 106/186 (56%), Gaps = 6/186 (3%) Query: 31 QRILEAAEALFEENGLEPVSMYGIAKRAGIGQGSLYRRFTDKGEICSALLSTSSERFLSR 90 Q+IL+ A+ALF + G+E VSM+ +AK AGIGQG+LYRR+ K +IC L+ +RF+ Sbjct: 10 QKILDTAQALFNKYGVEDVSMHQVAKTAGIGQGTLYRRYPSKSKICFTLMEAKIDRFMEG 69 Query: 91 LEQELQATTGASNSLEDLQSSIEQVVDFIDQHAELLQ-MIKSEFTGKKQLTQFEHPIFRR 149 L+ L+ + G + + L++ + +V+ ++ E L+ M+ S+ + + E+P F Sbjct: 70 LDVYLRNSEGETVA-HRLRTVMTKVILHFNEDLEWLRVMLTSDRLEETKNIMCENPPFTY 128 Query: 150 LNEVMTQRLRRAADDGLLIAVDPQFAATALIAVLSPDLYLYQQKLHNSTK---EEISRGV 206 L + + L AA+ G L+ +DP F + L ++ D+ LY + + S + EE R Sbjct: 129 LRLQIKRLLELAAERGELMPLDPLFTSVMLASLPRADIILYMRDMGYSAEQIAEEYCRSF 188 Query: 207 L-ALFV 211 + LF+ Sbjct: 189 VDPLFI 194 >Q1AX63.1 Q1AX63_RUBXD Transcriptional regulator, TetR family Length = 230 Score = 93.6 bits (231), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 61/180 (33%), Positives = 99/180 (55%), Gaps = 3/180 (1%) Query: 31 QRILEAAEALFEENGLEPVSMYGIAKRAGIGQGSLYRRFTDKGEICSALLSTSSERFLSR 90 +RI+EAA LF E G VSM IA+ AGIG+G+LYRRF KG +C ALL + + Sbjct: 38 RRIVEAARRLFAERGACAVSMEEIAREAGIGKGTLYRRFPHKGVLCEALLDEPTRQLQEE 97 Query: 91 LEQELQATTGASNSLEDLQSSIEQVVDFIDQHAELLQMIKSEFTGKKQLTQFEHPIFRRL 150 ++L A G S LE L + ++ F +++ +LL +G+ ++ + HP + Sbjct: 98 TMRDL-ADPG-SGPLEKLDRFLSRLARFTEENLDLLYGGHEPLSGEARVAYYSHPAHQWR 155 Query: 151 NEVMTQRLRRAADDGLLI-AVDPQFAATALIAVLSPDLYLYQQKLHNSTKEEISRGVLAL 209 + LR A +G L +D ++ + AL+A L DLY +Q+++ + E I+ G+ +L Sbjct: 156 RWTVLGLLRAAVREGALGEELDVEYLSEALLAPLGVDLYYHQRRVQGMSAERIAAGLRSL 215 >C2K3D5.1 C2K3D5_STAAU TetR family transcriptional regulator Length = 185 Score = 77.4 bits (189), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 52/186 (27%), Positives = 94/186 (50%), Gaps = 12/186 (6%) Query: 31 QRILEAAEALFEENGLEPVSMYGIAKRAGIGQGSLYRRFTDKGEICSALLSTSSERFLSR 90 QRI E A LF+E G+E +SM IAK GIG G+LYR F DK ++C ++ + F++ Sbjct: 10 QRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTLYRHFKDKSDLCYYVIQRDLDIFITH 69 Query: 91 LEQELQATTGASNSLEDLQSSIEQVVDFIDQHAELLQMIKSEFTGKKQLTQFEHPIFRRL 150 + Q ++ E +Q S++ ++ F H LLQ I++ G +L ++ ++ L Sbjct: 70 FK---QIKDDYHSNYEVMQVSLDYLLQFKIDHKALLQCIEA---GNNKLRFYQSAFYQEL 123 Query: 151 NEVMTQRLRRAADDGLLIAVDPQFAATALIAVLSPDLYLYQQKLHNSTKEEISRGVLALF 210 L ++ DD + +F L+ LS ++ +Q + N + E +L ++ Sbjct: 124 F-YFYYDLFKSDDD-----IYTKFKTDMLLQALSTRVFDFQIEHRNISIEAYRNYLLNIY 177 Query: 211 VTGIQR 216 + ++R Sbjct: 178 LDEVER 183 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,159,360,405 Number of extensions: 42354817 Number of successful extensions: 174397 Number of sequences better than 1.0e-05: 251 Number of HSP's gapped: 175359 Number of HSP's successfully gapped: 251 Length of query: 217 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 87 Effective length of database: 1,835,964,253 Effective search space: 159728890011 Effective search space used: 159728890011 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)