BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0580.0009.p (506 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DCX2.1 B1DCX2_9BACL Drug resistance transporter, EmrB/QacA sub... 548 e-154 B1DDS3.1 B1DDS3_9BACL Drug resistance transporter, EmrB/QacA sub... 425 e-117 B1DHW0.1 B1DHW0_9BACL Drug resistance transporter, EmrB/QacA sub... 401 e-110 Q5WI75.1 Q5WI75_BACSK Major facilitator (MFS) superfamily protein 382 e-104 B3K5L2.1 B3K5L2_9BACI Drug resistance transporter, EmrB/QacA sub... 380 e-103 >B1DCX2.1 B1DCX2_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 517 Score = 548 bits (1412), Expect = e-154, Method: Compositional matrix adjust. Identities = 274/427 (64%), Positives = 330/427 (77%), Gaps = 3/427 (0%) Query: 1 MEQELKS---DADFRLLSILVPLLAIIAGVFMVVLDSTAMNVALSTLVKDFHTELNTLQW 57 MEQ L + +F + SIL+PL+AII G FMV+LD+TAMNVALS LV+DFHT+L TLQW Sbjct: 1 MEQRLTDNPQEQEFSIKSILLPLVAIILGCFMVILDTTAMNVALSKLVEDFHTQLPTLQW 60 Query: 58 VVTGYMLAQASVIPLSGWLSDRFGAKTVFLTAVIVFTIGSILCATPSRAEWLIAFRVLQG 117 VTGYMLAQA+VIPL+GWLSDRFGAKTVFLT+VI+FTIGS+LCA P+ + LI FRV+QG Sbjct: 61 TVTGYMLAQAAVIPLAGWLSDRFGAKTVFLTSVILFTIGSLLCALPNDPQTLIMFRVIQG 120 Query: 118 LGGGCVLPVGMAYVYKLAPKSKXXXXXXXXXXPVLFAPAIGPVLSGWLVEYHSWRWXXXX 177 LGGG VLPV MAYVY+L+P +K P+L APAIGP+LSGWLVEYHSWRW Sbjct: 121 LGGGFVLPVAMAYVYRLSPPNKVGQVMGMLGVPILLAPAIGPILSGWLVEYHSWRWIFLI 180 Query: 178 XXXXXXXXXXXGSRKLPQSVRSQVPGMDKLGMILGPLAFASLSYGVSQGADSWTSDKTLI 237 G LP+ ++ G+D GMILGPLAFASLSYGV+QGA+SWTSDKTL Sbjct: 181 NLPVGIISVLFGLWSLPKLATKKLAGLDIPGMILGPLAFASLSYGVNQGAESWTSDKTLA 240 Query: 238 GLSVGMLALIAFVIAEFRSETPLLELRILRSIDFSTGILVQWIAQFGLYGAXXXXXXXXX 297 GL VG ALI F+I E R++ PLL+LR+ RSIDFS GI+VQWI QF L+GA Sbjct: 241 GLIVGGAALIVFIIVELRAKHPLLDLRVFRSIDFSFGIVVQWIVQFSLFGAIFLLPQFLQ 300 Query: 298 XARGFGAFDTGLTMLPQAIASGLMMPIAGILFDRIGVRWLVVCGLSLVSGALFQYSHVDL 357 RG+GAFDTGLT+ PQA+AS MMPI G LFDRIGVRWLVV GLSLVSGA+FQYSH+D+ Sbjct: 301 QGRGYGAFDTGLTLFPQALASACMMPIGGYLFDRIGVRWLVVIGLSLVSGAIFQYSHLDI 360 Query: 358 ATQGKDLILPLFMCGAGMGMMVMPMNTHLLNKAPSHLVNRVTSLTNSMQQVINALAVSTL 417 T +DL+LPL M G+GMG+M+MP+N+HL+NK P LV+RVTSLT++MQQVI++LAV+ L Sbjct: 361 HTGREDLLLPLIMAGSGMGLMMMPLNSHLINKTPRELVSRVTSLTSAMQQVISSLAVALL 420 Query: 418 VTILTSR 424 VT+LT R Sbjct: 421 VTVLTKR 427 >B1DDS3.1 B1DDS3_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 500 Score = 425 bits (1092), Expect = e-117, Method: Compositional matrix adjust. Identities = 214/422 (50%), Positives = 282/422 (66%), Gaps = 1/422 (0%) Query: 4 ELKSDADFRLLSILVPLLAIIAGVFMVVLDSTAMNVALSTLVKDFH-TELNTLQWVVTGY 62 E ++ + SI+VPLLAII G+FMV+LD TAMNVA+ L +DF L+ +QW VTGY Sbjct: 3 EATNNNSMSIKSIMVPLLAIIVGMFMVILDGTAMNVAIPKLREDFGGASLSLIQWTVTGY 62 Query: 63 MLAQASVIPLSGWLSDRFGAKTVFLTAVIVFTIGSILCATPSRAEWLIAFRVLQGLGGGC 122 LAQA+VIPL+GWLSDRFGAK ++LT++++FTIGS LCA + A+ LI FR++QGLGGG Sbjct: 63 ALAQAAVIPLAGWLSDRFGAKQIYLTSIVLFTIGSALCAFATTADQLIIFRIIQGLGGGM 122 Query: 123 VLPVGMAYVYKLAPKSKXXXXXXXXXXPVLFAPAIGPVLSGWLVEYHSWRWXXXXXXXXX 182 V P+ MA+ Y+L+P K P+L APA+GPVL+GWLV+Y +W W Sbjct: 123 VAPIAMAFTYRLSPPGKQGAVMGMIGIPMLLAPALGPVLAGWLVDYVTWHWIFIINIPVG 182 Query: 183 XXXXXXGSRKLPQSVRSQVPGMDKLGMILGPLAFASLSYGVSQGADSWTSDKTLIGLSVG 242 G R LP R VP +D LGMI PLAFA L+YGVS+G W S KTL GL VG Sbjct: 183 VVAVVLGIRTLPALARQTVPQLDVLGMIFAPLAFAGLAYGVSEGGTDWGSAKTLTGLIVG 242 Query: 243 MLALIAFVIAEFRSETPLLELRILRSIDFSTGILVQWIAQFGLYGAXXXXXXXXXXARGF 302 ++LI F+I E + PLLELR+ +S DFS GI+VQW++Q L+G A+G+ Sbjct: 243 GVSLIIFIIVELNRKQPLLELRVFKSADFSKGIVVQWVSQIALFGTLFLFPLFLQTAKGY 302 Query: 303 GAFDTGLTMLPQAIASGLMMPIAGILFDRIGVRWLVVCGLSLVSGALFQYSHVDLATQGK 362 A DTGL +LPQA+A+ + MPI G LFD+IG R LV+ GL++V+ F S++ Sbjct: 303 SALDTGLILLPQALAAAVFMPIGGKLFDKIGARPLVMSGLAIVTIGAFLLSNISSDATVG 362 Query: 363 DLILPLFMCGAGMGMMVMPMNTHLLNKAPSHLVNRVTSLTNSMQQVINALAVSTLVTILT 422 ILPL + GAGMG+ +MP+NTH++ AP LV+RVTSLTN+ QQV+ + AV+ L TIL Sbjct: 363 SYILPLILLGAGMGLSMMPLNTHIIQSAPPELVSRVTSLTNAAQQVMTSFAVAGLATILA 422 Query: 423 SR 424 R Sbjct: 423 RR 424 >B1DHW0.1 B1DHW0_9BACL Drug resistance transporter, EmrB/QacA subfamily Length = 491 Score = 401 bits (1031), Expect = e-110, Method: Compositional matrix adjust. Identities = 215/425 (50%), Positives = 277/425 (65%), Gaps = 6/425 (1%) Query: 3 QELKSDADFRLLSILVPLLAIIAGVFMVVLDSTAMNVALSTLVKDFHTELNTLQWVVTGY 62 Q + + F + SI+ PL+A+I G+FMV+LD TAMNVALS +K+F +L+ +QW TGY Sbjct: 7 QSVPDASTFTIRSIIAPLIAVIVGLFMVILDGTAMNVALSGFMKEFGKDLSVVQWTTTGY 66 Query: 63 MLAQASVIPLSGWLSDRFGAKTVFLTAVIVFTIGSILCATPSRAEWLIAFRVLQGLGGGC 122 LAQA+VIPL+GWLSDRFGAK +FL +V +FT GS+LCA E LI FR++QGLGGG Sbjct: 67 ALAQAAVIPLAGWLSDRFGAKRIFLVSVGMFTFGSLLCAFAGNIEMLILFRIIQGLGGGM 126 Query: 123 VLPVGMAYVYKLAPKSKXXXXXXXXXXPVLFAPAIGPVLSGWLVEYHSWRWXXXXXXXXX 182 V+P+ A +YKL+P K PVL APA+GPV++G L++Y +W W Sbjct: 127 VMPIAFAIIYKLSPPEKVGSVMGMLGIPVLIAPALGPVVAGALIDYATWHWIFLINIPLG 186 Query: 183 XXXXXXGSRKLPQSVRSQVPGMDKLGMILGPLAFASLSYGVSQGADSWTSDKTLIGLSVG 242 G R LP R +V +D G+I GPLAFA+LSYGVS+G SWTS +TL GL VG Sbjct: 187 IIGIIAGMRTLPNIERQEVKSLDVPGIIFGPLAFAALSYGVSEGGTSWTSAQTLTGLIVG 246 Query: 243 MLALIAFVIAEFRSETPLLELRILRSIDFSTGILVQWIAQFGLYGAXXXXXXXXXXARGF 302 +LI FV E RS+ PLLELR+ RS DFS GI+VQW Q L G +G Sbjct: 247 AASLIIFVFVELRSKQPLLELRVFRSRDFSKGIIVQWFTQIALMGTFFLIPMLLIQVQGH 306 Query: 303 GAFDTGLTMLPQAIASGLMMPIAGILFDRIGVRWLVVCGLSLVSGALFQYSHVDLATQGK 362 A ++GL MLPQAIAS + MPI G LFDRIG R L+V GLS+V A F S++ A G Sbjct: 307 TATESGLIMLPQAIASAVFMPIGGRLFDRIGARPLLVTGLSIVGVASFLLSNIS-ADDGL 365 Query: 363 DLIL---PLFMCGAGMGMMVMPMNTHLLNKAPSHLVNRVTSLTNSMQQVINALAVSTLVT 419 LIL LF G GMG+ +MPMNT+L+ +P LV RVTSLT++ QQV+ + A++ L T Sbjct: 366 SLILITSGLF--GVGMGLSMMPMNTYLIQASPPSLVGRVTSLTSAAQQVMTSFAIAGLST 423 Query: 420 ILTSR 424 LT++ Sbjct: 424 YLTTK 428 >Q5WI75.1 Q5WI75_BACSK Major facilitator (MFS) superfamily protein Length = 477 Score = 382 bits (982), Expect = e-104, Method: Compositional matrix adjust. Identities = 190/414 (45%), Positives = 264/414 (63%) Query: 11 FRLLSILVPLLAIIAGVFMVVLDSTAMNVALSTLVKDFHTELNTLQWVVTGYMLAQASVI 70 F + +I+ PL+A++ G+ MV+LDST +NVA ++F + L +QW TGY LA ++VI Sbjct: 7 FSIRTIIGPLIAVVVGMVMVILDSTIVNVAFPKFQQEFGSTLAVVQWTFTGYTLALSAVI 66 Query: 71 PLSGWLSDRFGAKTVFLTAVIVFTIGSILCATPSRAEWLIAFRVLQGLGGGCVLPVGMAY 130 PL+GW++D+FGAK +FLT V +FT+GS+LC E LI FR++QG+GGG V P+GMA Sbjct: 67 PLAGWMTDKFGAKRIFLTTVALFTLGSVLCGMAQTIEQLIIFRIVQGIGGGMVTPIGMAV 126 Query: 131 VYKLAPKSKXXXXXXXXXXPVLFAPAIGPVLSGWLVEYHSWRWXXXXXXXXXXXXXXXGS 190 +Y+L P K P+L APA+GPVLSGWL++Y SW+W G Sbjct: 127 IYRLTPSHKIGEVMGVLGIPILIAPALGPVLSGWLLDYVSWQWIFLINLPIGIIAIALGM 186 Query: 191 RKLPQSVRSQVPGMDKLGMILGPLAFASLSYGVSQGADSWTSDKTLIGLSVGMLALIAFV 250 + LP + +VP +D LGMIL PLAF+ L++GV +GA WTS +T++ L+VG AL+ + Sbjct: 187 KWLPAFPQGEVPKLDTLGMILAPLAFSMLAFGVGEGAVDWTSTRTVVALTVGAAALVGLI 246 Query: 251 IAEFRSETPLLELRILRSIDFSTGILVQWIAQFGLYGAXXXXXXXXXXARGFGAFDTGLT 310 + E R PLLELR+ +S DF+ GIL+ W+ Q L+G RG+ DTGL Sbjct: 247 VTELRHRQPLLELRVFKSSDFTRGILLLWLVQIALFGVMVLFPVYLQTVRGYSPLDTGLI 306 Query: 311 MLPQAIASGLMMPIAGILFDRIGVRWLVVCGLSLVSGALFQYSHVDLATQGKDLILPLFM 370 ML QA+ S L P+ G ++D++G R L + GL+ VS ALF SHV L+LPLFM Sbjct: 307 MLAQAVGSILFTPVGGRMYDKVGARPLALIGLTAVSAALFWLSHVSPEAGLTMLLLPLFM 366 Query: 371 CGAGMGMMVMPMNTHLLNKAPSHLVNRVTSLTNSMQQVINALAVSTLVTILTSR 424 G+GMG+++M +NTH+LN AP LV RVTSLT + Q VI + AV+ L L R Sbjct: 367 IGSGMGLIMMALNTHVLNSAPRDLVGRVTSLTTAAQHVITSFAVAGLTGFLAYR 420 >B3K5L2.1 B3K5L2_9BACI Drug resistance transporter, EmrB/QacA subfamily Length = 486 Score = 380 bits (975), Expect = e-103, Method: Compositional matrix adjust. Identities = 195/417 (46%), Positives = 274/417 (65%) Query: 8 DADFRLLSILVPLLAIIAGVFMVVLDSTAMNVALSTLVKDFHTELNTLQWVVTGYMLAQA 67 + F + I+ PLLA+I G FMV+LDST +NVA+ TLV+ F + L+T+QW + Y LA + Sbjct: 14 EKAFTIKDIIAPLLAVIIGTFMVLLDSTVVNVAVPTLVQYFDSPLHTMQWSIAAYTLALS 73 Query: 68 SVIPLSGWLSDRFGAKTVFLTAVIVFTIGSILCATPSRAEWLIAFRVLQGLGGGCVLPVG 127 +VIPL+GWLSD+F K VFL ++ +FTIGS+LCA AE LIAFRVLQG+GGG V P+G Sbjct: 74 AVIPLAGWLSDKFKPKRVFLISIGLFTIGSVLCAFAQTAEQLIAFRVLQGMGGGMVSPIG 133 Query: 128 MAYVYKLAPKSKXXXXXXXXXXPVLFAPAIGPVLSGWLVEYHSWRWXXXXXXXXXXXXXX 187 MA VY+LAP K P+L APA+GPVLSGWL+E+ SW+W Sbjct: 134 MAMVYRLAPPDKRGSVIGMFGIPLLLAPALGPVLSGWLIEFVSWQWIFWINLPIGMIGLF 193 Query: 188 XGSRKLPQSVRSQVPGMDKLGMILGPLAFASLSYGVSQGADSWTSDKTLIGLSVGMLALI 247 G + LP + P +D GMIL P+AFA ++YGVS+G SWTS +TL G+++G +AL+ Sbjct: 194 VGIKHLPSFPGKEAPTLDIAGMILAPMAFAMITYGVSEGGVSWTSPRTLFGITIGGIALL 253 Query: 248 AFVIAEFRSETPLLELRILRSIDFSTGILVQWIAQFGLYGAXXXXXXXXXXARGFGAFDT 307 F+ + R + PLLELR +S+DF+ GI++ WI Q ++G+ +GF T Sbjct: 254 LFIGSCLRQKQPLLELRAFKSVDFTRGIIISWIMQAAMFGSVLLFPLLLQQIKGFTPLTT 313 Query: 308 GLTMLPQAIASGLMMPIAGILFDRIGVRWLVVCGLSLVSGALFQYSHVDLATQGKDLILP 367 GL +LPQA+ S + MP+AG LFD++G R +V G++L++ AL S V + T +++ Sbjct: 314 GLILLPQAVGSMIFMPMAGKLFDKVGARPPLVAGMALMTVALLTMSFVRIDTPLFLIMIV 373 Query: 368 LFMCGAGMGMMVMPMNTHLLNKAPSHLVNRVTSLTNSMQQVINALAVSTLVTILTSR 424 LF G+GMG+ +M +NTH+LN P HLV+RVT LT++ QQVI + AV+ L+SR Sbjct: 374 LFFMGSGMGLSMMSLNTHVLNATPKHLVSRVTPLTSASQQVIASFAVAGFTGYLSSR 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,383,854,689 Number of extensions: 85549881 Number of successful extensions: 341710 Number of sequences better than 1.0e-05: 9452 Number of HSP's gapped: 326505 Number of HSP's successfully gapped: 9524 Length of query: 506 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 366 Effective length of database: 1,741,754,103 Effective search space: 637482001698 Effective search space used: 637482001698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)