BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0060.p (372 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5R1.1 B3K5R1_9BACI Spore germination protein 374 e-102 C0Z4B4.1 C0Z4B4_BREBN Spore germination protein 293 1e-77 A3IC05.1 A3IC05_9BACI Putative uncharacterized protein 285 7e-75 B1HPA0.1 B1HPA0_LYSSC Variant surface antigen E (VlpE prolipopro... 285 7e-75 A3I675.1 A3I675_9BACI Putative uncharacterized protein 271 8e-71 >B3K5R1.1 B3K5R1_9BACI Spore germination protein Length = 374 Score = 374 bits (960), Expect = e-102, Method: Compositional matrix adjust. Identities = 189/359 (52%), Positives = 273/359 (76%), Gaps = 1/359 (0%) Query: 5 VKISGRQFMVLTTLFTIGSAILIIPSGMAFLAKQDAWIAALVGVGAGFLLVWIYNTIATL 64 VKIS RQ +LT ++TIGSAILI+PSG A +A+QDAWIAALVG+G GF+ + + ++ Sbjct: 4 VKISLRQVSILTAMYTIGSAILIVPSGTANIARQDAWIAALVGIGLGFIFLAVQLQLSQK 63 Query: 65 YPRMTLVEIIETLLGKWLGKTISLLFIVTLFLSGPAAVLYDVGNFLTTQIMPETPAQSVN 124 YP L+++ E LLGKW+GKT++ L ++TLFLSGPA+VL+++ +F+T Q++PETP Q++ Sbjct: 64 YPESNLMQLTEKLLGKWIGKTVNFLLLLTLFLSGPASVLHEISDFMTIQMLPETPVQAII 123 Query: 125 ILFAVIIVMGARLGIETFARSAELLFPLFILLYISLVVLIAPEIKLENVQPVLETGIGPI 184 L+ ++V+G RLG+E ARSAE+LFP F+ L++S+ +L+ EIK EN P+ E+G PI Sbjct: 124 FLYGALVVLGVRLGLEVLARSAEMLFPWFLFLFLSMSILLLSEIKWENALPIFESGANPI 183 Query: 185 WPAALSFISVLFLPHIILLMIFP-TVNRIDNARKAFFNGSMFGGLMMVLIVALSILVLGP 243 +PAA+SF S FLP+I+L++I+P VNR KA + GS+ G +++ I+ L+ILVLGP Sbjct: 184 FPAAISFASTAFLPNIVLMIIYPGAVNRPAETSKAVYIGSLLGAGVLLTIIGLAILVLGP 243 Query: 244 DLTARSIFPSYSLAQKISIGNFLQRIEAIMAVMWFISLYFRATIYIYVIVLAIAQIFNLK 303 D+ AR+++PSY LA+KI+IGNFLQRIEA+MAVMWFI+L+FR ++Y Y I + I+QIFN+K Sbjct: 244 DIAARNMYPSYMLAKKINIGNFLQRIEAVMAVMWFITLFFRISLYFYAIAIGISQIFNIK 303 Query: 304 DYRPLILPLGMILVVMSVVIYPNVPYQQTWDGTVWLSYTLSIGXXXXXXXXCIHMVRKL 362 +Y+PL++PLG IL +SV IYPNVPY+ WD W+ YT+++G +H +RKL Sbjct: 304 NYQPLVVPLGWILAAVSVFIYPNVPYKLEWDAKTWIPYTITVGFIFPLVLFGMHGLRKL 362 >C0Z4B4.1 C0Z4B4_BREBN Spore germination protein Length = 363 Score = 293 bits (751), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 162/356 (45%), Positives = 235/356 (66%), Gaps = 2/356 (0%) Query: 6 KISGRQFMVLTTLFTIGSAILIIPSGMAFLAKQDAWIAALVGVGAGFLLVWIYNTIATLY 65 IS RQFM+L LF IG AIL +PS A AKQDAWI+A +G G GFLL ++Y +++ Y Sbjct: 6 HISARQFMILVALFGIGDAILYVPSLTATAAKQDAWISAFMGWGEGFLLTYLYVSLSMRY 65 Query: 66 PRMTLVEIIETLLGKWLGKTISLLFIVTLFLSGPAAVLYDVGNFLTTQIMPETPAQSVNI 125 P ++ + E +LGKW+GK +++LFI F+ + +L ++G+F+TTQIM ETP + + + Sbjct: 66 PNKSIFQFSEEILGKWMGKAVAVLFITYFFIDA-SLMLMEIGDFVTTQIMQETPIEIILV 124 Query: 126 LFAVIIVMGARLGIETFARSAELLFPLFILLYISLVVLIAPEIKLENVQPVLETGIGPIW 185 LF VIIVMG R G E F+R ELLFP F+LL+ L+ I+P+IK+EN++PV+ GI P+ Sbjct: 125 LFLVIIVMGVRSGPEAFSRVGELLFPYFLLLFFVLIAFISPQIKIENLKPVVAHGIAPVI 184 Query: 186 PAALSFISVLFLPHIILLMIFPTVNRIDNARKAFFNGSMFGGLMMVLIVALSILVLGPDL 245 ++ L L +IL+++FP V R A KA+ G +F + +I +++ILVLG D+ Sbjct: 185 GGNFRYVGYL-LETVILIVMFPLVQRPARAAKAYIWGILFANFCLTMITSVAILVLGADI 243 Query: 246 TARSIFPSYSLAQKISIGNFLQRIEAIMAVMWFISLYFRATIYIYVIVLAIAQIFNLKDY 305 T +PSY+LAQKISIGNFL+RIE +MAV+WFI+L+ + TI Y IA L+DY Sbjct: 244 TVLLAYPSYTLAQKISIGNFLERIEVLMAVIWFITLFVKITICYYATSCGIAYTMGLRDY 303 Query: 306 RPLILPLGMILVVMSVVIYPNVPYQQTWDGTVWLSYTLSIGXXXXXXXXCIHMVRK 361 R L LPLGMI++V+++ PN PY T+ +W+ Y+L+ G + +RK Sbjct: 304 RQLTLPLGMIMIVVALATMPNRPYFDTFVSEIWMPYSLTYGLFLPVLLLSVGALRK 359 >A3IC05.1 A3IC05_9BACI Putative uncharacterized protein Length = 469 Score = 285 bits (728), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 160/340 (47%), Positives = 235/340 (69%), Gaps = 5/340 (1%) Query: 1 VSKQVKISGRQFMVLTTLFTIGSAILIIPSGMAFLAKQDAWIAALVGVGAGFLLVWIYNT 60 + K+V IS RQF ++T L +IG+AILIIPS +A +KQDAWIAA +GV L+V ++ T Sbjct: 1 MEKEV-ISSRQFTIITFLLSIGTAILIIPSSIASSSKQDAWIAASIGVVLSLLVVKLFVT 59 Query: 61 IATLYPRMTLVEIIETLLGKWLGKTISLLFI-VTLFLSGPAAVLYDVGNFLTTQIMPETP 119 + P + +E E LLG++ GK + F+ +TLF G +LY +G F+ T++MPETP Sbjct: 60 LGDQTPTLNFIEANEKLLGRFFGKITVVGFLSLTLFAGG--ELLYFIGAFMKTEVMPETP 117 Query: 120 AQSVNILFAVIIVMGARLGIETFARSAELLFPLFILLYISLVVLIAPEIKLENVQPVLET 179 + +LF +II+ LGIE FARSAE+LFP+F+L++I VV I P+IK EN+QP++E Sbjct: 118 TMAFALLFIIIIMYATYLGIENFARSAEILFPMFVLIFIFFVVSITPQIKFENIQPIMEA 177 Query: 180 GIGPIWPAALSFISVLFLPHIILLMIFP-TVNRIDNARKAFFNGSMFGGLMMVLIVALSI 238 I + + F+SV ++LLMI+P +VN +++K F+ G + GG ++V ++ LSI Sbjct: 178 SKTSILYSIIRFVSVFSFSLVMLLMIYPASVNVQQSSKKGFYIGMLLGGFVLVTLITLSI 237 Query: 239 LVLGPDLTARSIFPSYSLAQKISIGNFLQRIEAIMAVMWFISLYFRATIYIYVIVLAIAQ 298 LVLGP TA FPSY+LAQ+ISIGNFLQRIE IMA MW +S++ R +Y Y V+ IAQ Sbjct: 238 LVLGPANTASRTFPSYALAQRISIGNFLQRIEIIMAFMWIVSIFIRTFMYFYTTVVGIAQ 297 Query: 299 IFNLKDYRPLILPLGMILVVMSVVIYPNVPYQQTWDGTVW 338 I LKD+RPLILP+G+I+V +S +++P++ + ++ +W Sbjct: 298 IMKLKDHRPLILPMGIIMVGLSQIVHPDIVHSNIYNDEIW 337 >B1HPA0.1 B1HPA0_LYSSC Variant surface antigen E (VlpE prolipoprotein) Length = 624 Score = 285 bits (728), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 154/339 (45%), Positives = 236/339 (69%), Gaps = 4/339 (1%) Query: 7 ISGRQFMVLTTLFTIGSAILIIPSGMAFLAKQDAWIAALVGVGAGFLLVWIYNTIATLYP 66 IS RQF ++T+L +IG+AILIIPS +A +KQDAWIAA +GV L+V ++ T+ P Sbjct: 6 ISSRQFTIITSLLSIGTAILIIPSTIASSSKQDAWIAASIGVVLSLLVVKLFLTLGNQTP 65 Query: 67 RMTLVEIIETLLGKWLGKTISLLFI-VTLFLSGPAAVLYDVGNFLTTQIMPETPAQSVNI 125 + +E E + G++ GK + F+ +TLF G +LY +G F+ T++MPETP + + Sbjct: 66 TLNFIEANEKIFGRFFGKITVIGFLSLTLFSGG--ELLYFIGIFMKTEVMPETPTLAFAL 123 Query: 126 LFAVIIVMGARLGIETFARSAELLFPLFILLYISLVVLIAPEIKLENVQPVLETGIGPIW 185 LF++II+ A LG+ETFARSAE+LFP+FIL++I +V I P+IK EN+QP++E ++ Sbjct: 124 LFSIIIMYAAYLGLETFARSAEILFPMFILIFIFFIVCITPQIKFENIQPIMEVSKTSLF 183 Query: 186 PAALSFISVLFLPHIILLMIFP-TVNRIDNARKAFFNGSMFGGLMMVLIVALSILVLGPD 244 + + F+SV ++LLMI+P +VN +++K F+ G++ GG ++V ++ L ILVLG Sbjct: 184 YSIIRFMSVFSFSLVMLLMIYPASVNVQQSSKKGFYIGTILGGFVLVTLITLCILVLGAA 243 Query: 245 LTARSIFPSYSLAQKISIGNFLQRIEAIMAVMWFISLYFRATIYIYVIVLAIAQIFNLKD 304 TA FPSY+LAQ+ISIGNFLQRIE IMA MW +S++ R +Y Y V+ IAQI LKD Sbjct: 244 NTASRTFPSYALAQRISIGNFLQRIEIIMAFMWIVSIFIRTFMYFYTTVVGIAQIMKLKD 303 Query: 305 YRPLILPLGMILVVMSVVIYPNVPYQQTWDGTVWLSYTL 343 +RPLILP+G+I++ +S +++P++ + ++ +W Y+ Sbjct: 304 HRPLILPMGIIMIGLSQIVHPDIVHSDNYNNEIWPIYSF 342 >A3I675.1 A3I675_9BACI Putative uncharacterized protein Length = 373 Score = 271 bits (693), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 145/339 (42%), Positives = 226/339 (66%), Gaps = 3/339 (0%) Query: 1 VSKQVKISGRQFMVLTTLFTIGSAILIIPSGMAFLAKQDAWIAALVGVGAGFLLVWIYNT 60 + KQ+ IS RQF +L FT+G+ ILIIP+ +A KQDAWI A +G LL+ +Y T Sbjct: 1 MEKQL-ISSRQFTILVIFFTVGTGILIIPASLALEVKQDAWIVATIGTILSLLLIKLYIT 59 Query: 61 IATLYPRMTLVEIIETLLGKWLGKTISLLFIVTLFLSGPAAVLYDVGNFLTTQIMPETPA 120 ++ ++L E+ E LG ++GK ISL F++ LS +LY +GNFL T++MPETP Sbjct: 60 LSNKMMNLSLTEVTEKFLGSFIGKIISLAFVLLTLLSS-GELLYFIGNFLQTEVMPETPP 118 Query: 121 QSVNILFAVIIVMGARLGIETFARSAELLFPLFILLYISLVVLIAPEIKLENVQPVLETG 180 +ILF+ II+ A GIE FAR+ E+LFP+F+ ++I + ++P+I ++NVQPV E Sbjct: 119 VVFSILFSFIILFAAYQGIEVFARTLEILFPIFLFVFIIFLFFVSPQIDIKNVQPVFEVS 178 Query: 181 IGPIWPAALSFISVLFLPHIILLMIFPT-VNRIDNARKAFFNGSMFGGLMMVLIVALSIL 239 I P+ + L F+ + P I+LLMIFP+ +N +++ +K F+ G + GG ++ +ALSIL Sbjct: 179 IPPLIYSVLHFMGLFSFPLIVLLMIFPSAINNLESGKKGFYIGIIVGGFIITAFIALSIL 238 Query: 240 VLGPDLTARSIFPSYSLAQKISIGNFLQRIEAIMAVMWFISLYFRATIYIYVIVLAIAQI 299 VLG T FPSY+LAQ++S+GNFLQR+E IMA +W ++++ R+ +Y Y ++ ++ I Sbjct: 239 VLGVTNTTLRTFPSYTLAQRVSVGNFLQRVEIIMAFIWMVTIFVRSFMYFYASLIGLSHI 298 Query: 300 FNLKDYRPLILPLGMILVVMSVVIYPNVPYQQTWDGTVW 338 LK++RPLILPLGMI + +S +++ N+ + ++ W Sbjct: 299 LKLKEHRPLILPLGMISIALSQIVHANIIHSDQYNQQTW 337 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.144 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,946,384,609 Number of extensions: 74606773 Number of successful extensions: 303707 Number of sequences better than 1.0e-05: 713 Number of HSP's gapped: 302682 Number of HSP's successfully gapped: 713 Length of query: 372 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 235 Effective length of database: 1,770,017,148 Effective search space: 415954029780 Effective search space used: 415954029780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)