BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0058.p (408 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5R3.1 B3K5R3_9BACI Germination protein, Ger(X)C family 427 e-117 B1DF38.1 B1DF38_9BACL Germination protein, Ger(X)C family 391 e-107 C0Z585.1 C0Z585_BREBN Putative spore germination protein 358 6e-97 C0Z4B2.1 C0Z4B2_BREBN Spore germination protein 332 3e-89 B1HP98.1 B1HP98_LYSSC Spore germination protein KC 320 2e-85 >B3K5R3.1 B3K5R3_9BACI Germination protein, Ger(X)C family Length = 401 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 210/408 (51%), Positives = 282/408 (69%), Gaps = 7/408 (1%) Query: 1 MKRKASFXXXXXXXXXXXTGWWXXXXNRKELNELAIAVGMGIDKQGDQFRVSVQVVDPGE 60 MKR + +G W NR+ELNELAIAV G+D ++R+S+QV PG+ Sbjct: 1 MKRILALLLLHIVIMLLLSGCW----NRRELNELAIAVAAGVDWVDGRYRLSIQVAIPGQ 56 Query: 61 VSXXXXXXXXXXXXXXXLYTSEADTVFEAVRKITTLSPRKIYFPHLRICVISESMAMEGI 120 V+ LYT+E DT+FEA R++T +SPRKIYF HLR+ ++ E+MA EGI Sbjct: 57 VTAKRSGGPQAPAT---LYTAEGDTIFEAARRMTQISPRKIYFSHLRMFLMDEAMAREGI 113 Query: 121 AKPLDFLSRDHEFRSDFYLVITKGASAEDTLKIMTPLDPIPADQLFSSLESSKKNWSPST 180 AK LD LSRDHEFR DFYL ITKGA+ E+TLKIMTP++ IPA++L+ SL+ S+K WSPS Sbjct: 114 AKVLDLLSRDHEFRPDFYLAITKGATVEETLKIMTPMETIPANKLYDSLQISEKVWSPSM 173 Query: 181 TVTLDQLMADLISEGKQPILTGLQIVGDQNSGDTKKNVEKISPPTRLQFSGIAVFKKDRL 240 VTLD+L+ D+ S GK P+LTGL I G++ G TKKNVE I P++L++SG AVFK D+L Sbjct: 174 AVTLDELINDITSIGKHPVLTGLVITGNREIGKTKKNVENIQTPSQLKYSGFAVFKNDKL 233 Query: 241 IGWLNEIESKGYNFILDKVQSSVGFVDCPKGGKVAMEMIRNHTSMKGNVYRGEPRININL 300 IGWLNE +S+GY FI+ V+SSVGF+ C GKV +E +R +KG + + P ++I L Sbjct: 234 IGWLNEKDSRGYRFIMGDVKSSVGFIPCHNDGKVVVETLRTDAKLKGRIVQSRPEMSIKL 293 Query: 301 QIEANVGEVECSDLDLTKVSTIYDLQKRGEEKIAGVMESAIRKAQKSYKADIFGFGEAIH 360 IE N+G VECS LDL++ T+ +L++ EE++ +ES I K Q K DIFGFGE H Sbjct: 294 NIEGNIGSVECSGLDLSQPETVRELEEEMEERLTRFLESVIDKVQTELKVDIFGFGETFH 353 Query: 361 RSNPKAWKSLKQNWDDEYFPELQVNIKVDFKIRRLGTNGSSFLNEIKK 408 RS+PK WK ++ W+D YFP L+ ++ VD+KIRR GT +SFLN+IK+ Sbjct: 354 RSHPKQWKEMQDAWNDRYFPNLKTHVNVDYKIRRTGTTNNSFLNDIKE 401 >B1DF38.1 B1DF38_9BACL Germination protein, Ger(X)C family Length = 400 Score = 391 bits (1005), Expect = e-107, Method: Compositional matrix adjust. Identities = 185/388 (47%), Positives = 274/388 (70%), Gaps = 10/388 (2%) Query: 20 GWWXXXXNRKELNELAIAVGMGIDKQGD-QFRVSVQVVDPGEVSXXXXXXXXXXXXXXXL 78 G W NR+ELN+LAIAV +GIDK G+ Q++VSVQVVDPGEV+ Sbjct: 21 GCW----NRRELNDLAIAVAIGIDKDGENQYQVSVQVVDPGEVAANNRTMTRTPVT---F 73 Query: 79 YTSEADTVFEAVRKITTLSPRKIYFPHLRICVISESMAMEGIAKPLDFLSRDHEFRSDFY 138 Y + T+FEA+RK+TT+SPR IY HLR+ VI E +A EGI L+++SR +E R+DFY Sbjct: 74 YQARGRTIFEAIRKMTTVSPRPIYSAHLRMLVIGEELAKEGIGDALEYMSRYYEHRTDFY 133 Query: 139 LVITKGASAEDTLKIMTPLDPIPADQLFSSLESSKKNWSPSTTVTLDQLMADLISEGKQP 198 + I + +A TLKI+T L+ IPA+Q++SSL +S+K W+P+ ++TLDQL+ D++S+GKQP Sbjct: 134 IAIARETTAASTLKILTHLEAIPANQMYSSLLNSQKQWAPTASITLDQLIMDIVSKGKQP 193 Query: 199 ILTGLQIVGDQNSGDTKKNVEKISPPTRLQFSGIAVFKKDRLIGWLNEIESKGYNFILDK 258 ++TGL++ GDQ G +++NVE I+ LQ+SG+AV +D+LIGWLNE+ES+GY+FI + Sbjct: 194 VVTGLRLRGDQEKGMSRENVESIASAAHLQYSGLAVINQDKLIGWLNEVESRGYSFIHND 253 Query: 259 VQSSVGFVDCPKGGKVAMEMIRNHTSMKGNVYRGEPRININLQIEANVGEVECSDLDLTK 318 V ++VG + CP GGK+ +E+IR+ T K GEP I++ ++IEA++GEV C +DLT+ Sbjct: 254 VHNTVGVLKCPDGGKMTIEVIRSKTIPKAKFVNGEPEIHLAIRIEASIGEVLCK-IDLTQ 312 Query: 319 VSTIYDLQKRGEEKIAGVMESAIRKAQKSYKADIFGFGEAIHRSNPKAWKSLKQNWDDEY 378 S+IY ++ + E+ I G ++ I +AQ+ YK DIFGFG+ +HR+NP WK ++ NWD Sbjct: 313 KSSIYKMEHKSEDAIRGFVQKTIDQAQEKYKVDIFGFGDVVHRANPAYWKKVENNWDS-V 371 Query: 379 FPELQVNIKVDFKIRRLGTNGSSFLNEI 406 F ++ V + V IR+ GT +SF+ E+ Sbjct: 372 FQKMPVKLDVVVNIRQTGTVNNSFMQEM 399 >C0Z585.1 C0Z585_BREBN Putative spore germination protein Length = 393 Score = 358 bits (919), Expect = 6e-97, Method: Compositional matrix adjust. Identities = 173/383 (45%), Positives = 259/383 (67%), Gaps = 10/383 (2%) Query: 20 GWWXXXXNRKELNELAIAVGMGIDKQGDQFRVSVQVVDPGEVSXXXXXXXXXXXXXXXLY 79 G W +RKELN+LAIAVG+GIDK ++VSVQVVDP EV+ L+ Sbjct: 18 GCW----DRKELNDLAIAVGIGIDKVQTGYQVSVQVVDPAEVAANKGKPGRAPVT---LF 70 Query: 80 TSEADTVFEAVRKITTLSPRKIYFPHLRICVISESMAMEGIAKPLDFLSRDHEFRSDFYL 139 T A T+FEAVRK+TT+SPRKIY H R+ + E +A EGIA +DF+SRDHEFR+DFY+ Sbjct: 71 TMNAPTIFEAVRKMTTISPRKIYMAHTRMLIFGEEVAREGIAPVIDFVSRDHEFRTDFYI 130 Query: 140 VITKGASAEDTLKIMTPLDPIPADQLFSSLESSKKNWSPSTTVTLDQLMADLISEGKQPI 199 I +G +A + L+++TPL+ IP+ ++F+SLE+S K W+P+ VT+DQL+ D S+GK+ + Sbjct: 131 AIARGTTARNILRVITPLEQIPSTEMFASLETSSKVWAPTAAVTMDQLITDFTSKGKEAV 190 Query: 200 LTGLQIVGDQNSGDTKKNVEKISPPTRLQFSGIAVFKKDRLIGWLNEIESKGYNFILDKV 259 LTG+++ G GD + NVE+I P LQ++ +A F+ D++I WL+E +SK Y++I KV Sbjct: 191 LTGVRLTGPAREGDKRNNVEQIDTPVLLQYTTLATFRGDKMINWLSEADSKAYSYITGKV 250 Query: 260 QSSVGFVDCPKGGKVAMEMIRNHTSMKGNVYRGEPRININLQIEANVGEVECSDLDLTKV 319 +S+VG + C GG + +E++ ++ + G P + +++ +E ++GEV+C LDLT+ Sbjct: 251 KSTVGSMPCKGGGLLTLEVMHADAKIEATMKNGTPEVIVHIALENDIGEVQCK-LDLTEE 309 Query: 320 STIYDLQKRGEEKIAGVMESAIRKAQKSYKADIFGFGEAIHRSNPKAWKSLKQNWDDEYF 379 TI +L+K E+ + + AI A+K+ DI+GFGEAIHR PK WK + NW ++ F Sbjct: 310 DTIKELEKEAEKSLEALATKAIANAKKN-GVDIYGFGEAIHRQYPKVWKKIGSNW-EKGF 367 Query: 380 PELQVNIKVDFKIRRLGTNGSSF 402 +L V++K D KIRRLGT ++F Sbjct: 368 SKLPVHVKADVKIRRLGTINNAF 390 >C0Z4B2.1 C0Z4B2_BREBN Spore germination protein Length = 400 Score = 332 bits (852), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 165/408 (40%), Positives = 261/408 (63%), Gaps = 9/408 (2%) Query: 1 MKRKASFXXXXXXXXXXXTGWWXXXXNRKELNELAIAVGMGIDKQGDQFRVSVQVVDPGE 60 MKRK F W NR+ELN+LAI +G GIDK ++ +S Q+V+PG Sbjct: 1 MKRKGVFLLTLALLVLLTGCW-----NRRELNDLAIVMGTGIDKIDGEYVISTQIVNPGG 55 Query: 61 VSXXXXXXXXXXXXXXXLYTSEADTVFEAVRKITTLSPRKIYFPHLRICVISESMAMEGI 120 V+ Y+ A ++FE +RK+TT++PR++YF H+++ V+ E +A EG Sbjct: 56 VASKEGGGMNQSSVAT--YSMRASSIFEGIRKMTTVTPRRLYFSHIQVLVLGEQIAKEGA 113 Query: 121 AKPLDFLSRDHEFRSDFYLVITKGASAEDTLKIMTPLDPIPADQLFSSLESSKKNWSPST 180 + LD L RDHE R DFY+++ K A+A L ++ PL+ IPA +L+ SLE+++ W Sbjct: 114 KEVLDLLFRDHEVRPDFYVILAKDATALQILSMIDPLEKIPATKLYKSLETAESAWGSIV 173 Query: 181 TVTLDQLMADLISEGKQPILTGLQIVGDQNSGDTKKNVEKISPPTRLQFSGIAVFKKDRL 240 ++ LD+ +ADL+ +G +P+L G+ G + G + N +K S + L++ I VFK D+L Sbjct: 174 SIQLDEFIADLLRDGIEPVLVGVNSKGKVHMGTSGGNSQKESQSSLLRYHEIGVFKGDKL 233 Query: 241 IGWLNEIESKGYNFILDKVQSSVGFVDCPKGGKVAMEMIRNHTSMKGNVYRGEPRININL 300 GW NE ES G+++I D+V S+V + C K K+ +E+IR+ T++ G V +G+P++ +++ Sbjct: 234 RGWFNEEESIGFSYITDRVDSTVEEIPCDKNNKIVIEIIRSKTTVNGKVEKGKPQVEVSI 293 Query: 301 QIEANVGEVECSDLDLTKVSTIYDLQKRGEEKIAGVMESAIRKAQKSYKADIFGFGEAIH 360 E NVGEV CS+ DL+ TIY L+ E++I G + SAI KAQK Y++D+FGFG++I+ Sbjct: 294 FAEGNVGEVACSN-DLSDPKTIYKLEADVEKQIRGKIVSAIHKAQKQYRSDVFGFGQSIY 352 Query: 361 RSNPKAWKSLKQNWDDEYFPELQVNIKVDFKIRRLGTNGSSFLNEIKK 408 +++PK W+ +K++WD + FP L+V IK D K+RR G G+SF+ +KK Sbjct: 353 KNDPKGWEKVKEDWDSQ-FPNLKVKIKTDVKLRRTGKVGNSFIPFMKK 399 >B1HP98.1 B1HP98_LYSSC Spore germination protein KC Length = 399 Score = 320 bits (819), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 151/390 (38%), Positives = 256/390 (65%), Gaps = 9/390 (2%) Query: 19 TGWWXXXXNRKELNELAIAVGMGIDKQGDQFRVSVQVVDPGEVSXXXXXXXXXXXXXXXL 78 +G W +++ELNELAI V +GIDK D+F ++VQ+VDP EVS Sbjct: 18 SGCW----SKRELNELAIVVALGIDKVEDEFEITVQIVDPSEVSMRQASTQRTPVIS--- 70 Query: 79 YTSEADTVFEAVRKITTLSPRKIYFPHLRICVISESMAMEGIAKPLDFLSRDHEFRSDFY 138 Y S +T+FEA+RK+TT++ RK YF HL++ ++ E +A EGI+ LD ++RDHEFR DF Sbjct: 71 YHSRGETIFEAIRKMTTIAARKPYFAHLQVVILGEDLAKEGISDSLDLIARDHEFRKDFD 130 Query: 139 LVITKGASAEDTLKIMTPLDPIPADQLFSSLESSKKNWSPSTTVTLDQLMADLISEGKQP 198 ++++ A+A++ L ++TP++ +PA+++ +SL S+K W + + +D+L+ L + Sbjct: 131 VIMSHEATAKEVLNVLTPIEKVPANKILNSLNVSEKVWGSTIAINIDELVTRLNEKDNGA 190 Query: 199 ILTGLQIVGDQNSGDTKKNVEKISPPTRLQFSGIAVFKKDRLIGWLNEIESKGYNFILDK 258 +++ ++I GD+ G K NV+++ P L+++G+AVFK DR IG L+E ESK +F+ DK Sbjct: 191 LISAIEIHGDKRLGADKTNVQRVKTPVLLKYAGLAVFKGDRYIGLLSEGESKSISFLSDK 250 Query: 259 VQSSVGFVDCPKGGKVAMEMIRNHTSMKGNVYRGEPRININLQIEANVGEVECSDLDLTK 318 +QS++ V+CPK G ++ E+ ++ T +KG+ G+P+I++ + +E NVGEVEC D++LTK Sbjct: 251 IQSTIEIVNCPKQGTLSTEITQSSTKVKGSFQNGKPQIDVQIDVEQNVGEVEC-DINLTK 309 Query: 319 VSTIYDLQKRGEEKIAGVMESAIRKAQKSYKADIFGFGEAIHRSNPKAWKSLKQNWDDEY 378 T+ + K+ + I +E + K Q+ Y+ D+ G+G+A+HR++ K WK +K +W Sbjct: 310 NKTMDYINKKTAQGIDERIEKTLEKIQQQYQVDVLGYGDALHRADAKQWKKIKNDW-PTI 368 Query: 379 FPELQVNIKVDFKIRRLGTNGSSFLNEIKK 408 FP+L V+++V + LGT +S L + K+ Sbjct: 369 FPDLPVHVRVKVTTQGLGTMQNSLLKKPKE 398 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,101,768,949 Number of extensions: 80228134 Number of successful extensions: 199026 Number of sequences better than 1.0e-05: 362 Number of HSP's gapped: 198513 Number of HSP's successfully gapped: 363 Length of query: 408 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 270 Effective length of database: 1,760,596,133 Effective search space: 475360955910 Effective search space used: 475360955910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)