BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0057.p (530 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5R4.1 B3K5R4_9BACI GerA spore germination protein 664 0.0 A3I672.1 A3I672_9BACI Spore germination protein 591 e-167 B1DF37.1 B1DF37_9BACL GerA spore germination protein 587 e-165 C0Z4B1.1 C0Z4B1_BREBN Spore germination protein 564 e-158 B1HP97.1 B1HP97_LYSSC Spore germination protein KA 563 e-158 >B3K5R4.1 B3K5R4_9BACI GerA spore germination protein Length = 531 Score = 664 bits (1713), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/531 (60%), Positives = 400/531 (75%), Gaps = 1/531 (0%) Query: 1 MRFRKSNPLNKVKKPQVQHSESQPAHSEEPISASLGQNLQQIRQTVGNSTDIIIREVHIG 60 MR+R+ + + S + +S L NL IR+T+GNSTDI+IRE+ + Sbjct: 1 MRYRRRTGHSNKNNTKDTPSAAATKKDSNALSPELESNLNHIRRTLGNSTDIVIREIPLD 60 Query: 61 KDRSQIIAILYIDGLVDKNIVXXXXXXXXXXXXXXXEDVNRRIQISSNVLQILKDYALTI 120 + +A++Y DGL D+ I+ N ++L++ + Sbjct: 61 EGEKHYMAVVYTDGLADQKIISESIMSAVADIAKSRARENTEYGKPLEAQKVLEEISFIS 120 Query: 121 GDIRDVTDFETLYNALLSGNAILLVDGETQGIVASAGGWKDRGVTEPASENVVRGPREGF 180 +I+ ++D++TL+++LLSG+AI+LVDG +GI A A GWKDRGV E ++ENVVRGPRE F Sbjct: 121 KEIQSISDYKTLFHSLLSGDAIVLVDGLDRGIAAGARGWKDRGVMETSAENVVRGPRESF 180 Query: 181 SETLRTNTALIRRKIKDPNLWLETKQIGRVTKTDVAVMYIKGIVNDKLVEEVHERLNRID 240 +E +RTNTALIRRKIKDP+LW+ETKQIGR+T+TDV +MYI GI N+K+V EV RL RID Sbjct: 181 TEAVRTNTALIRRKIKDPHLWMETKQIGRITQTDVVMMYIDGIANEKIVGEVRTRLERID 240 Query: 241 VDSILETGYIEELIQDETFTPFPTVYNSERPDVIAANLLEGKIAILVDGTPYVLIVPALF 300 VDSILE+GYIEEL+QD+T TPFPT+YN+ERPDV+AA LLEGKIAI+VDGTP+VLIVPALF Sbjct: 241 VDSILESGYIEELVQDKTLTPFPTIYNTERPDVVAAELLEGKIAIIVDGTPFVLIVPALF 300 Query: 301 VSFLHAAEDYYQRADISSFIRLLRYIGVFISLFSPSLYIAITTFHQEMLPTSLLIGLAAQ 360 VSFLHAAEDYYQRADISSF+R+LRY+ +FISL PSLY+AITTFH EMLPT LL GLA Q Sbjct: 301 VSFLHAAEDYYQRADISSFLRILRYLSIFISLLGPSLYVAITTFHHEMLPTRLLFGLAGQ 360 Query: 361 REAVPFPAFIEALMMELTFEILREAGVRMPKYIGAAVSIVGTLVIGQAAVEAGXXXXXXX 420 RE +PFPAF+EA++ME+TFEILREAGVR+PK IG+A+SIVGTLVIGQAAVEAG Sbjct: 361 REQIPFPAFVEAVLMEVTFEILREAGVRLPKNIGSAISIVGTLVIGQAAVEAGIISPAMV 420 Query: 421 XXXXXXXXXXXXLPAYNMSIAFRMLRFPLMGLAASFGLFGIIVGGIALILHLCSLRSFGV 480 +P++NMSIAFRMLRFPLM LA SFGLFGI+VG IA+ILHLCSLRSFG+ Sbjct: 421 IVVAITAISSFVVPSFNMSIAFRMLRFPLMALAGSFGLFGILVGVIAIILHLCSLRSFGI 480 Query: 481 PYMSPLAPLIPADSKDTLIRLPHWMMVSRPRLLNQKNVNRR-NFASRKSRE 530 PYMSP APLIP KD+++RLPHWM+++RPRL+NQ N RR + +KSRE Sbjct: 481 PYMSPFAPLIPEGQKDSILRLPHWMLINRPRLINQDNEKRRKDTPPQKSRE 531 >A3I672.1 A3I672_9BACI Spore germination protein Length = 520 Score = 591 bits (1523), Expect = e-167, Method: Compositional matrix adjust. Identities = 287/487 (58%), Positives = 362/487 (74%), Gaps = 5/487 (1%) Query: 31 ISASLGQNLQQIRQTVGNSTDIIIREVHIGKDRSQIIAILYIDGLVDKNIVXXXXXXXXX 90 +S SL +N++ I+ ++GNS DIIIRE IGK IAI+Y DGL D + Sbjct: 32 LSTSLPKNIKTIKNSIGNSNDIIIREFLIGKPIEVPIAIIYTDGLADNTSITDFILESII 91 Query: 91 XXXXXXEDVNRRIQISSNVLQILKDYALTIGDIRDVTDFETLYNALLSGNAILLVDGETQ 150 D + ++ LK Y LT+G I++V+DF +L+NA+LSG +LL++G Sbjct: 92 SDFEVTSDTPLQ-----EIVDRLKTYCLTVGSIKNVSDFPSLFNAILSGETVLLLEGLAI 146 Query: 151 GIVASAGGWKDRGVTEPASENVVRGPREGFSETLRTNTALIRRKIKDPNLWLETKQIGRV 210 GI S KDR +TEP +E V+RGPRE F+ETLRTNTALIRRKIK PNLW++T+ IG V Sbjct: 147 GISTSTKSSKDRSITEPVTEAVIRGPRESFTETLRTNTALIRRKIKSPNLWIKTRVIGEV 206 Query: 211 TKTDVAVMYIKGIVNDKLVEEVHERLNRIDVDSILETGYIEELIQDETFTPFPTVYNSER 270 T+T++A+MYI GI N+K+V+EV +RL+RID+D ILE+GYIE+LIQD FT FPTVYNSER Sbjct: 207 TQTEIAIMYINGIANEKIVKEVLKRLDRIDIDGILESGYIEQLIQDSKFTIFPTVYNSER 266 Query: 271 PDVIAANLLEGKIAILVDGTPYVLIVPALFVSFLHAAEDYYQRADISSFIRLLRYIGVFI 330 PDVIA LLEGKIAILVDGTP+VLIVPALF SFL +AEDYYQ A +SSFIRL+R++G+ + Sbjct: 267 PDVIAGELLEGKIAILVDGTPFVLIVPALFTSFLQSAEDYYQSAFVSSFIRLIRFLGISL 326 Query: 331 SLFSPSLYIAITTFHQEMLPTSLLIGLAAQREAVPFPAFIEALMMELTFEILREAGVRMP 390 +L +PSLYI++TTFHQEM+PT LLI + AQRE VPFPAF+EAL+ME+ FE+LREAG+RMP Sbjct: 327 ALVAPSLYISLTTFHQEMIPTRLLISIYAQREGVPFPAFVEALIMEIAFEVLREAGLRMP 386 Query: 391 KYIGAAVSIVGTLVIGQAAVEAGXXXXXXXXXXXXXXXXXXXLPAYNMSIAFRMLRFPLM 450 + IG+A+SIVGTL+IGQAAVEAG PAY +S + R+LRFPLM Sbjct: 387 RMIGSAISIVGTLIIGQAAVEAGIVSAAMVIIVALTAICSFLFPAYGLSNSIRILRFPLM 446 Query: 451 GLAASFGLFGIIVGGIALILHLCSLRSFGVPYMSPLAPLIPADSKDTLIRLPHWMMVSRP 510 LAA FGLFG+ VG + LI+HLCSLRSFGVPYMSP APLIP D KD LI P +++RP Sbjct: 447 VLAAMFGLFGVFVGIMLLIIHLCSLRSFGVPYMSPFAPLIPKDQKDALIIFPRRSLLTRP 506 Query: 511 RLLNQKN 517 RL++Q N Sbjct: 507 RLISQIN 513 >B1DF37.1 B1DF37_9BACL GerA spore germination protein Length = 486 Score = 587 bits (1512), Expect = e-165, Method: Compositional matrix adjust. Identities = 289/486 (59%), Positives = 361/486 (74%), Gaps = 13/486 (2%) Query: 38 NLQQIRQTVGNSTDIIIREVHIG-KDRSQIIAILYIDGLVDKNIVXXXXXXXXXXXXXXX 96 ++ I++ +GNS DI+ +E I DRSQ + + Y DGLV+ V Sbjct: 5 DVSAIKEKLGNSNDIVFKEFQIEYADRSQRVDLFYADGLVNAGSVQDFILETLMVEFP-- 62 Query: 97 EDVNRRIQISSNVLQILKDYALTIGDIRDVTDFETLYNALLSGNAILLVDGETQGIVASA 156 ++ +N L LT GD+ +VT ET+Y L SG+ IL+ D T+ + + Sbjct: 63 -------KLDTNQHPFLD--LLTAGDVEEVTSTETMYYDLFSGHVILIWD-STKILSIAM 112 Query: 157 GGWKDRGVTEPASENVVRGPREGFSETLRTNTALIRRKIKDPNLWLETKQIGRVTKTDVA 216 G KDR V+E +E V RGP+E F+E++RTNTAL+RRKIK+PNLWLE +IG+VT+T V+ Sbjct: 113 NGIKDRAVSEAMTETVSRGPKESFTESIRTNTALVRRKIKNPNLWLENMRIGKVTQTSVS 172 Query: 217 VMYIKGIVNDKLVEEVHERLNRIDVDSILETGYIEELIQDETFTPFPTVYNSERPDVIAA 276 +MY+KGIV+DK+V+EV RL RID+D ILE+GYIEELIQD ++PFPT+YNSERPDVIAA Sbjct: 173 IMYVKGIVSDKIVDEVRNRLTRIDIDGILESGYIEELIQDSAYSPFPTMYNSERPDVIAA 232 Query: 277 NLLEGKIAILVDGTPYVLIVPALFVSFLHAAEDYYQRADISSFIRLLRYIGVFISLFSPS 336 LLEGK+AILVDGTP+VL+VPALF+SF+H+AEDYYQRADISS IR+LR++G+FI+L PS Sbjct: 233 ELLEGKVAILVDGTPHVLVVPALFISFMHSAEDYYQRADISSLIRILRFVGIFIALLGPS 292 Query: 337 LYIAITTFHQEMLPTSLLIGLAAQREAVPFPAFIEALMMELTFEILREAGVRMPKYIGAA 396 LYIAITTFHQEMLPT LLIGLAAQRE +PFPAFIEAL+ME+TFEILREAGVRMP+ +G A Sbjct: 293 LYIAITTFHQEMLPTQLLIGLAAQREGIPFPAFIEALIMEITFEILREAGVRMPRTMGQA 352 Query: 397 VSIVGTLVIGQAAVEAGXXXXXXXXXXXXXXXXXXXLPAYNMSIAFRMLRFPLMGLAASF 456 +SIVGTLVIGQAAV+AG +PA NMSI+ RM+RF LMGLAASF Sbjct: 353 ISIVGTLVIGQAAVDAGIVSAAMVIVVSITAISSFVVPAMNMSISIRMIRFALMGLAASF 412 Query: 457 GLFGIIVGGIALILHLCSLRSFGVPYMSPLAPLIPADSKDTLIRLPHWMMVSRPRLLNQK 516 GL+GII+G AL+LHLCSLR+FGVPYMSP P I D+KDTL RLPHW M R RL++Q Sbjct: 413 GLYGIILGIFALVLHLCSLRTFGVPYMSPFGPFIAEDTKDTLFRLPHWAMRKRLRLISQN 472 Query: 517 NVNRRN 522 N+ R N Sbjct: 473 NITRGN 478 >C0Z4B1.1 C0Z4B1_BREBN Spore germination protein Length = 503 Score = 564 bits (1453), Expect = e-158, Method: Compositional matrix adjust. Identities = 271/500 (54%), Positives = 359/500 (71%), Gaps = 7/500 (1%) Query: 30 PISASLGQNLQQIRQTVGNSTDIIIREVHIGKDRSQIIAILYIDGLVDKNIVXXXXXXXX 89 P SL +NL+++++ +GNSTD++ R+V + ++ ILY +GLVDKN++ Sbjct: 10 PFHCSLDENLEKVKKELGNSTDLVTRQVRLEAVDQIVVGILYTEGLVDKNMISSLMESLM 69 Query: 90 XXXXXXXEDVNRRIQISSNVLQILKDYALTIGDIRDVTDFETLYNALLSGNAILLVDGET 149 + + L ++KD+ LTIG + +V DF+ LY+ALL+G+ ++LVDG + Sbjct: 70 LHAKKA--HIPAEAECEERSLILMKDFLLTIGGVEEVYDFDHLYDALLTGDTVILVDGYS 127 Query: 150 QGIVASAGGWKDRGVTEPASENVVRGPREGFSETLRTNTALIRRKIKDPNLWLETKQIGR 209 G +A+ W+DRGV E + + VVRG RE FSE LRTNTAL+RR+I + NLWLET QIG Sbjct: 128 NGFIANTKQWEDRGVQETSVQTVVRGSREAFSENLRTNTALVRRRINNRNLWLETVQIGD 187 Query: 210 VTKTDVAVMYIKGIVNDKLVEEVHERLNRIDVDSILETGYIEELIQDETFTPFPTVYNSE 269 +T+T VA+MYIKG+V D +V EV ERL +I++D ILE+G IEE+IQD TFTPFPT+YN+E Sbjct: 188 MTQTKVAMMYIKGVVQDSVVAEVKERLGKIEIDGILESGNIEEMIQDHTFTPFPTIYNTE 247 Query: 270 RPDVIAANLLEGKIAILVDGTPYVLIVPALFVSFLHAAEDYYQRADISSFIRLLRYIGVF 329 RPD +AA LLEG++AILVDGTP VL+VPA+FV F + EDYY RAD +R+LR+I F Sbjct: 248 RPDSVAAGLLEGRVAILVDGTPIVLLVPAVFVQFFQSPEDYYMRADF-GILRILRFISFF 306 Query: 330 ISLFSPSLYIAITTFHQEMLPTSLLIGLAAQREAVPFPAFIEALMMELTFEILREAGVRM 389 I+L PS+Y+AITTFHQEML T+LL+ +AAQRE VPFPA +EAL+ME TFE+LREAGVRM Sbjct: 307 IALLGPSIYVAITTFHQEMLQTNLLMSIAAQREGVPFPAAVEALLMEFTFEMLREAGVRM 366 Query: 390 PKYIGAAVSIVGTLVIGQAAVEAGXXXXXXXXXXXXXXXXXXXLPAYNMSIAFRMLRFPL 449 P+ +G+A+SIVG LV+G+AAV+AG P+++MSI R+LRF L Sbjct: 367 PRAVGSAISIVGALVLGEAAVQAGLVSPATVIVVSITAITSFVFPSFSMSIPIRLLRFGL 426 Query: 450 MGLAASFGLFGIIVGGIALILHLCSLRSFGVPYMSPLAPLIPADSKDTLIRLPHWMMVSR 509 + LAASFG GI VG IAL +HLC+LRSFGVPYM+PLAP+ AD KDTL+R+P WMM +R Sbjct: 427 IALAASFGFIGIFVGMIALCIHLCTLRSFGVPYMAPLAPVNLADQKDTLLRIPIWMMHTR 486 Query: 510 PRLLNQKNVNRRNFASRKSR 529 P L QKN R RKS+ Sbjct: 487 PHFLRQKNSVRE----RKSK 502 >B1HP97.1 B1HP97_LYSSC Spore germination protein KA Length = 514 Score = 563 bits (1452), Expect = e-158, Method: Compositional matrix adjust. Identities = 279/505 (55%), Positives = 365/505 (72%), Gaps = 6/505 (1%) Query: 27 SEEPISASLGQNLQQIRQTVGNSTDIIIREVHIGKDRSQIIAILYIDGLVDKNIVXXXXX 86 +++ ++ SL +N++ I+ T+G+S DIIIRE+ IG+ +AI+ IDGL DK ++ Sbjct: 15 TDDLLNTSLAKNIKVIQNTLGHSNDIIIREITIGEAEPHRLAIISIDGLSDKTMISDSVI 74 Query: 87 XXXXXXXXXXEDVNRRIQISSNVLQILKDYALTIGDIRDVTDFETLYNALLSGNAILLVD 146 + + + Q ++ LT+GD+ ++ F TLY+A+L+G ++L+D Sbjct: 75 DKLMA-----NIEEEQEIEITTINQYVRSSYLTVGDVMNIEYFTTLYHAILNGETVILLD 129 Query: 147 GETQGIVASAGGWKDRGVTEPASENVVRGPREGFSETLRTNTALIRRKIKDPNLWLETKQ 206 G +GIV S KDR VTEP++E+V+RGPRE F+ETLRTNTALIRRKIK PNLW++++ Sbjct: 130 GFAEGIVTSTKSAKDRAVTEPSTESVIRGPRESFTETLRTNTALIRRKIKSPNLWIKSRV 189 Query: 207 IGRVTKTDVAVMYIKGIVNDKLVEEVHERLNRIDVDSILETGYIEELIQDETFTPFPTVY 266 IG VT+TDVAVMYI GI +DK+V EV RL+RI++D ILE+GYIE+ IQD + FPTVY Sbjct: 190 IGEVTQTDVAVMYIHGIASDKIVAEVLARLDRINIDGILESGYIEDFIQDSKNSMFPTVY 249 Query: 267 NSERPDVIAANLLEGKIAILVDGTPYVLIVPALFVSFLHAAEDYYQRADISSFIRLLRYI 326 NSERPDVIA LL+GK+AILVDGTP+VL+VPALF SFL +AEDYYQ ISS IR+LR+ Sbjct: 250 NSERPDVIAGELLDGKVAILVDGTPFVLVVPALFTSFLQSAEDYYQHWMISSLIRILRFF 309 Query: 327 GVFISLFSPSLYIAITTFHQEMLPTSLLIGLAAQREAVPFPAFIEALMMELTFEILREAG 386 G+ ++L +PSLY+AITTFHQEMLPT +LI +A+QRE VPFPA +EAL+MEL FEILREAG Sbjct: 310 GISLALVAPSLYVAITTFHQEMLPTPMLISIASQREGVPFPAVVEALIMELAFEILREAG 369 Query: 387 VRMPKYIGAAVSIVGTLVIGQAAVEAGXXXXXXXXXXXXXXXXXXXLPAYNMSIAFRMLR 446 +RMP+ IG AVSIVGTLVIGQAAV+AG PAY +S R+LR Sbjct: 370 LRMPRTIGPAVSIVGTLVIGQAAVDAGVVSAVMVIIVALTAICSFLFPAYGLSNTIRVLR 429 Query: 447 FPLMGLAASFGLFGIIVGGIALILHLCSLRSFGVPYMSPLAPLIPADSKDTLIRLPHWMM 506 FPLM LAA FGLFG++ G + +ILHLCS+RSFGVPY+SP AP I D KD + LP + Sbjct: 430 FPLMILAAMFGLFGVMFGIMVIILHLCSIRSFGVPYLSPFAPFIIQDQKDAFLLLPRRHL 489 Query: 507 VSRPRLLNQKNVNR-RNFASRKSRE 530 + RPRL+NQKNV R + +R R+ Sbjct: 490 LKRPRLVNQKNVVRGHKYVTRTGRK 514 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.139 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,758,946,100 Number of extensions: 110185218 Number of successful extensions: 297142 Number of sequences better than 1.0e-05: 1090 Number of HSP's gapped: 294759 Number of HSP's successfully gapped: 1092 Length of query: 530 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 389 Effective length of database: 1,732,333,088 Effective search space: 673877571232 Effective search space used: 673877571232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)