BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0579.0020.p (194 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DET3.1 B1DET3_9BACL Transcriptional regulator, TetR family 363 6e-99 B3KCY4.1 B3KCY4_9BACI Regulatory protein TetR 98 5e-19 Q4H4G4.1 Q4H4G4_BACCI Possible transcriptional regulator 63 2e-08 Q8GCB5.1 Q8GCB5_BACCI BtrW protein 62 3e-08 B3K861.1 B3K861_9BACI Regulatory protein TetR 55 4e-06 >B1DET3.1 B1DET3_9BACL Transcriptional regulator, TetR family Length = 194 Score = 363 bits (931), Expect = 6e-99, Method: Compositional matrix adjust. Identities = 173/194 (89%), Positives = 183/194 (94%) Query: 1 MCPRTKEQNELIRIQRREQILDVAARSYFRTGGSFDIRDVAREAGLGYGTVYHYYPNRHL 60 MCPRTKEQNE IRIQRREQILDVAARSYFRTGGSFDIRDVAREAGLGYGTVYHYYPNRHL Sbjct: 1 MCPRTKEQNEFIRIQRREQILDVAARSYFRTGGSFDIRDVAREAGLGYGTVYHYYPNRHL 60 Query: 61 LIEDVLDSGFERCEHVIAEWADISGSHPEDRQLLTYCKALLQLWQSDARAYLVYKMAAEH 120 LIEDVL+SGFERCE IAE A+I GS +DRQL TYCKALL LWQSDARAYLVYKMA+EH Sbjct: 61 LIEDVLESGFERCEQFIAELANIGGSPTDDRQLFTYCKALLLLWQSDARAYLVYKMASEH 120 Query: 121 YAGLPEKDRHHAKRRFMERLYVPLQAIAQCEDDGVDHMLAVLVGCCGLYYYAGNFDLDVD 180 YAGLPEKDRHHAKR+FMERLYVPLQ++AQCEDDG+DHMLAVLVGCCGL+YYAGN DLDVD Sbjct: 121 YAGLPEKDRHHAKRKFMERLYVPLQSLAQCEDDGIDHMLAVLVGCCGLHYYAGNSDLDVD 180 Query: 181 RIARLAMQAIMKES 194 RIARLA+QAI KES Sbjct: 181 RIARLALQAITKES 194 >B3KCY4.1 B3KCY4_9BACI Regulatory protein TetR Length = 246 Score = 97.8 bits (242), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 76/243 (31%), Positives = 98/243 (40%), Gaps = 58/243 (23%) Query: 1 MCPRTKEQNELIRIQRREQILDVAARSYFRTGGSFDIRDVAREAGLGYGTVYHYYPNRHL 60 M PRTKEQNE IR++R QI AA + G +IRDVA +A LGYGTVYHYY N+ Sbjct: 1 MTPRTKEQNEEIRLRRLAQIRKAAADVFLNKGPLLEIRDVAAQAELGYGTVYHYYSNKGD 60 Query: 61 LIEDVLDSGFER----------------CEHVIAE----W-----ADISGSHPEDRQLLT 95 L+ D+L ER C E W ADI S D + + Sbjct: 61 LLHDLLWDALERTGEWLEVPLNAPPKVLCASASGEAPAGWPLATAADIGNSAAADSRSTS 120 Query: 96 YCKA--------------LLQLWQSDARAYLVYKMAAEHYAGLPEKDRHHAKRRFMERLY 141 A LLQLW D +L++K+A E +A LPE F + Sbjct: 121 LETAAGAELSPVAAAGVRLLQLWAEDHALFLLHKLAGEGFAALPEARSAPLTAAFRREVL 180 Query: 142 VPLQAIAQCEDDG-------------------VDHMLAVLVGCCGLYYYAGNFDLDVDRI 182 P+ A+ + E + +LA LVGC L G + I Sbjct: 181 APIAALLESERAANEAAAAVANAAEPPYRLQRAEMLLAALVGCASLSLRRGMLHEEARDI 240 Query: 183 ARL 185 R Sbjct: 241 VRF 243 >Q4H4G4.1 Q4H4G4_BACCI Possible transcriptional regulator Length = 217 Score = 62.8 bits (151), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 31/87 (35%), Positives = 50/87 (57%), Gaps = 1/87 (1%) Query: 1 MCPRTKEQNELIRIQRREQILDVAARSYFRTG-GSFDIRDVAREAGLGYGTVYHYYPNRH 59 M PRTKEQNE IR QR+ ++L A + Y G + ++ D+A AGL +G VY+Y+ N+ Sbjct: 1 MSPRTKEQNEAIRNQRKREVLQAAVQVYVEKGYAAAEMGDIADRAGLAHGLVYYYFKNKK 60 Query: 60 LLIEDVLDSGFERCEHVIAEWADISGS 86 L ++ ++ E + + + GS Sbjct: 61 ALFRELYETMMEESKCFTKSYFEQKGS 87 >Q8GCB5.1 Q8GCB5_BACCI BtrW protein Length = 217 Score = 61.6 bits (148), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 31/87 (35%), Positives = 50/87 (57%), Gaps = 1/87 (1%) Query: 1 MCPRTKEQNELIRIQRREQILDVAARSYFRTG-GSFDIRDVAREAGLGYGTVYHYYPNRH 59 M PRTKEQNE IR QR+ ++L A + Y G + ++ D+A AGL +G VY+Y+ N+ Sbjct: 1 MSPRTKEQNEAIRNQRKREVLQAAVQVYVEKGYAAAEMGDIADRAGLAHGLVYYYFKNKK 60 Query: 60 LLIEDVLDSGFERCEHVIAEWADISGS 86 L ++ ++ E + + + GS Sbjct: 61 ALFRELYETMMEGSKCFTKSYFEQKGS 87 >B3K861.1 B3K861_9BACI Regulatory protein TetR Length = 201 Score = 54.7 bits (130), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 35/131 (26%), Positives = 66/131 (50%), Gaps = 8/131 (6%) Query: 1 MCPRTKEQNELIRIQRREQILDVAARSYFRTG--GSFDIRDVAREAGLGYGTVYHYYPNR 58 M P +EQ IR +R+EQI+ A + + + G G+ + +A EAG+ +G +YHY+ ++ Sbjct: 1 MAPLNEEQLHQIRDERKEQIMRAAIKVFSKRGIFGT-KMSMIAGEAGVSHGLLYHYFKSK 59 Query: 59 HLLIEDVLDSGFERCEHVIAEWADISGSHPEDRQLLTYCKALLQLWQSDARAYLVYKMAA 118 L ++ + H +++ D+ G+ E LLT + Q D ++ + A Sbjct: 60 DELFITLVQWSMDEARHALSDIYDVPGTPLEKITLLTSM-----ILQEDDNSHFMLIQQA 114 Query: 119 EHYAGLPEKDR 129 G+PE+ + Sbjct: 115 RTSDGVPEEAK 125 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,184,385,543 Number of extensions: 48399629 Number of successful extensions: 139419 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 139706 Number of HSP's successfully gapped: 5 Length of query: 194 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 66 Effective length of database: 1,854,806,283 Effective search space: 122417214678 Effective search space used: 122417214678 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)