BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0578.0010.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KC00.1 B3KC00_9BACI NmrA family protein 283 1e-74 C0Z4S3.1 C0Z4S3_BREBN Putative uncharacterized protein 267 6e-70 Q2TNI4.1 Q2TNI4_9ENTR Triphenylmethane reductase 218 6e-55 B1NFR6.1 B1NFR6_9PSED Triphenylmethane reductase 218 6e-55 A6N8W4.1 A6N8W4_9BACT Triphenylmethane reductase 216 1e-54 >B3KC00.1 B3KC00_9BACI NmrA family protein Length = 274 Score = 283 bits (723), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 136/268 (50%), Positives = 180/268 (67%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M+I+ITGA+G+LG LII+ LL + I+A VR +D+A +++G+ VR DYD P +L Sbjct: 1 MSIVITGASGRLGRLIIKELLHHVQPDQIVACVRQMDSAAALEEQGIAVRFCDYDDPASL 60 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 ++AFAG ++LL ISS H DD VRL QH +V++AAK A V+HILYT FAFP+ + + + Sbjct: 61 KEAFAGASRLLFISSPHPDDTVRLRQHAHVVEAAKKANVSHILYTGFAFPEHSDMSMTHL 120 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLAR 180 H TE AI +G+ YT LR+GLY+DF+ L L A+ +G L PG WQFNAVTR DLA Sbjct: 121 HLATEHAIRTTGIPYTFLRSGLYMDFIEALDLQAAMSTGHLRVHPGSWQFNAVTRTDLAA 180 Query: 181 ATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHTADASVQHWIYHFLNSI 240 A A VL+G GH ++ YEL A WTF L L+EL G P+ VQHWI+ FL I Sbjct: 181 AIAGVLSGEGHSNKTYELVAPSVWTFNDLVSSLSELTGTPISLCLTNEVQHWIFSFLRKI 240 Query: 241 DTKSTSNDLEQLMGQPVTSLKESIAPFL 268 DT STS DLE L+G+P SLK S+ ++ Sbjct: 241 DTSSTSGDLENLLGRPPASLKASLQSYI 268 >C0Z4S3.1 C0Z4S3_BREBN Putative uncharacterized protein Length = 271 Score = 267 bits (683), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 127/269 (47%), Positives = 177/269 (65%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M+I+ITGA G+LG+LIIE LL + +IA V LD A ++ G+ VR ADYD P +L Sbjct: 1 MSILITGATGKLGSLIIEQLLKRVERERLIACVHRLDKAIQLRELGIEVRQADYDDPLSL 60 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 + +FAG +KLL ISS H+DD VR+ QH++VI+AAK A + H++YTSFAF + N + + Sbjct: 61 ERSFAGGSKLLFISSPHTDDTVRVRQHSHVIEAAKKANITHLVYTSFAFLEKANISLTHL 120 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLAR 180 H TE AI +G+ YT LRNGLY D + G+ +AI +G L T PG W F+ VTR DL+ Sbjct: 121 HLATEHAIRTTGIPYTFLRNGLYSDVIAAFGIQQAIETGELITYPGKWSFHTVTREDLSL 180 Query: 181 ATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHTADASVQHWIYHFLNSI 240 A A V++ GHE++ YEL A++ W F LAEVL GK + H D + W+YHFL I Sbjct: 181 AAATVISTEGHENQTYELTASRAWNFVELAEVLAARTGKSIRHREDPVAESWLYHFLMQI 240 Query: 241 DTKSTSNDLEQLMGQPVTSLKESIAPFLN 269 D ST+ DLE+++G+P SL++ + + Sbjct: 241 DASSTTTDLERIIGKPTASLEQMVRQIME 269 >Q2TNI4.1 Q2TNI4_9ENTR Triphenylmethane reductase Length = 287 Score = 218 bits (554), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 121/288 (42%), Positives = 171/288 (59%), Gaps = 19/288 (6%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M+I +TGA GQLG L+I++LL ++ IIA VRN++ A+ D+GV VR DY++PE+L Sbjct: 1 MSIAVTGATGQLGGLVIQHLLKKVPASQIIAIVRNVEKASTLADQGVEVRHGDYNQPESL 60 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 Q AFAGV+KLL IS H D+ + + QH NV+ AA+ AGV HI YT +AF + + V Sbjct: 61 QKAFAGVSKLLFISGPHYDNTLLIVQHANVVKAARDAGVKHIAYTGYAFAEESIIPLAHV 120 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLAR 180 H TE AI + + YT LRN LY DF GL +I SG + T G N+VTR +LA Sbjct: 121 HLATEYAIRTTNIPYTFLRNALYTDFFVNEGLRASIESGAIVTNAGSGIVNSVTRNELAL 180 Query: 181 ATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHTAD-------------- 226 A A VL GHE++ Y L + Q WTF LA++L+E++GK V+H Sbjct: 181 AAATVLTEEGHENKTYNLVSNQPWTFDELAQILSEVSGKKVVHQPVSFEEEKNFLVNAGV 240 Query: 227 ----ASVQHWIYHFLNSIDTKSTSNDLEQLMGQPVTSLKESIAPFLNL 270 A + IY ++ + TS+DL++L+G +T LKE++ L + Sbjct: 241 PEPFAEITAAIYDAISKGEASKTSDDLQKLIGS-LTPLKETVKQALKM 287 >B1NFR6.1 B1NFR6_9PSED Triphenylmethane reductase Length = 287 Score = 218 bits (554), Expect = 6e-55, Method: Compositional matrix adjust. Identities = 121/288 (42%), Positives = 171/288 (59%), Gaps = 19/288 (6%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M+I +TGA GQLG L+I++LL ++ IIA VRN++ A+ D+GV VR DY++PE+L Sbjct: 1 MSIAVTGATGQLGGLVIQHLLKKVPASQIIAIVRNVEKASTLADQGVEVRHGDYNQPESL 60 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 Q AFAGV+KLL IS H D+ + + QH NV+ AA+ AGV HI YT +AF + + V Sbjct: 61 QKAFAGVSKLLFISGPHYDNTLLIVQHANVVKAARDAGVKHIAYTGYAFAEESIIPLAHV 120 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLAR 180 H TE AI + + YT LRN LY DF GL +I SG + T G N+VTR +LA Sbjct: 121 HLATEYAIRTTNIPYTFLRNALYTDFFVNEGLRASIESGAIVTNAGSGIVNSVTRNELAL 180 Query: 181 ATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHTAD-------------- 226 A A VL GHE++ Y L + Q WTF LA++L+E++GK V+H Sbjct: 181 AAATVLTEEGHENKTYNLVSNQPWTFDELAQILSEVSGKKVVHQPVSFEEEKNFLVNAGV 240 Query: 227 ----ASVQHWIYHFLNSIDTKSTSNDLEQLMGQPVTSLKESIAPFLNL 270 A + IY ++ + TS+DL++L+G +T LKE++ L + Sbjct: 241 PEPFAEITAAIYDAISKGEASKTSDDLQKLIGS-LTPLKETVKQALKM 287 >A6N8W4.1 A6N8W4_9BACT Triphenylmethane reductase Length = 303 Score = 216 bits (551), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 120/288 (41%), Positives = 170/288 (59%), Gaps = 19/288 (6%) Query: 1 MTIMITGAAGQLGNLIIENLLAELSDGTIIAGVRNLDTATHFKDKGVVVRCADYDRPETL 60 M+I +TGA GQLG L+I++LL ++ IIA VRN++ A+ D+GV VR DY++PE+L Sbjct: 17 MSIAVTGATGQLGGLVIQHLLKKVPASQIIAIVRNVEKASTLADQGVEVRHGDYNQPESL 76 Query: 61 QDAFAGVNKLLLISSSHSDDAVRLTQHTNVIDAAKAAGVAHILYTSFAFPQAGNKGTSSV 120 Q AFAGV+KLL IS H D+ + + QH NV+ AA+ GV HI YT +AF + + V Sbjct: 77 QKAFAGVSKLLFISGPHYDNTLLIVQHANVVKAARDVGVKHIAYTGYAFAEESIIPLAHV 136 Query: 121 HGLTEQAILDSGMEYTILRNGLYIDFVGVLGLNEAIRSGVLATQPGDWQFNAVTRRDLAR 180 H TE AI + + YT LRN LY DF GL +I SG + T G N+VTR +LA Sbjct: 137 HLATEYAIRTTNIPYTFLRNALYTDFFVNEGLRASIESGAIVTNAGSGIVNSVTRNELAL 196 Query: 181 ATAKVLAGSGHEHRIYELAAAQTWTFAGLAEVLTELAGKPVIHTAD-------------- 226 A A VL GHE++ Y L + Q WTF LA++L+E++GK V+H Sbjct: 197 AAATVLTEEGHENKTYNLVSNQPWTFDELAQILSEVSGKKVVHQPVSFEEEKNFLVNAGV 256 Query: 227 ----ASVQHWIYHFLNSIDTKSTSNDLEQLMGQPVTSLKESIAPFLNL 270 A + IY ++ + TS+DL++L+G +T LKE++ L + Sbjct: 257 PEPFAEITAAIYDAISKGEASKTSDDLQKLIGS-LTPLKETVKQALKM 303 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,507,947,333 Number of extensions: 59219898 Number of successful extensions: 228912 Number of sequences better than 1.0e-05: 676 Number of HSP's gapped: 227926 Number of HSP's successfully gapped: 677 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)