BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0578.0006.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DJ10.1 B1DJ10_9BACL Metallophosphoesterase 315 4e-84 C3B0A0.1 C3B0A0_BACMY Metallophosphoesterase 276 3e-72 C3AIJ3.1 C3AIJ3_BACMY Metallophosphoesterase 276 3e-72 C3BH22.1 C3BH22_9BACI Metallophosphoesterase 275 4e-72 C2W4Y6.1 C2W4Y6_BACCE Metallophosphoesterase 263 2e-68 >B1DJ10.1 B1DJ10_9BACL Metallophosphoesterase Length = 383 Score = 315 bits (808), Expect = 4e-84, Method: Compositional matrix adjust. Identities = 168/345 (48%), Positives = 219/345 (63%), Gaps = 4/345 (1%) Query: 21 NFYIGQHLWYLLETWLPGISAAVYWPVFLLIAFAYMIGMVPWPKA--VKPAARLFKVIGS 78 N+YIG + W L T A Y + +++FA++IG A ++ A K IGS Sbjct: 20 NWYIGWNGWVYLSTLFDLEHAWPYGIIVGILSFAWLIGRFGQHSASPIRQVAYFLKNIGS 79 Query: 79 YYLACMEFAIIVLPLTDLLYWVLGLMGVQRTAFTAEAGXXXXXXXXXFLIWGSRNAWSTV 138 Y+ A ME+ +++LPL DL W+L L V A G LI G+ NAW+ + Sbjct: 80 YWFAVMEYGLLILPLADLAVWLLTLASVSYKTAVASVGTVTFVLLASILIRGAWNAWTPI 139 Query: 139 VRNHPIQIGKSAGTSAPLTIAVASDLHLGNIVGNRHLRRMVAEMNRMQPDLILLAGDVLD 198 VRN+ + I K AGT L I VASD+HLG +VGN+HL R+V+ M M+PDLILL GDV+D Sbjct: 140 VRNYDLTIPKQAGTIRKLRIGVASDIHLGTVVGNKHLDRLVSHMEEMKPDLILLPGDVID 199 Query: 199 DSIEPFIRNGMCEEMRKLQARHGVYAVLGNHEYYGGGXXXXXTELMDSIGIKVLQDE-VV 257 DSI+PFIR M M KL+A G+YAVLGNHEY GG + M++IGI+VL DE ++ Sbjct: 200 DSIDPFIRRNMAATMSKLKAPLGIYAVLGNHEYIGGHIQEYIAQ-MNAIGIRVLLDENIL 258 Query: 258 EAAGLYIVGRKDKTAESTEAAGRKSVEALLDGLDLSRPVIMMDHQPTGFGIAAQAGVDVL 317 YIVGRKD A ++ GR + L+ LD +RP+IMMDHQP+ AA G+D+ Sbjct: 259 VRDSFYIVGRKDLAARHSDPDGRLPISELISDLDKARPLIMMDHQPSDLEQAAINGIDLS 318 Query: 318 LSGHTHRGQIAPNHWITRRLFELDWGYLRKDKLHVVVSSGYGTWG 362 LSGHTHRGQ+APNH ITRR+FELDWGY K LH +VSSG+G+WG Sbjct: 319 LSGHTHRGQMAPNHLITRRIFELDWGYKLKGSLHAIVSSGFGSWG 363 >C3B0A0.1 C3B0A0_BACMY Metallophosphoesterase Length = 350 Score = 276 bits (705), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 151/342 (44%), Positives = 205/342 (59%), Gaps = 13/342 (3%) Query: 22 FYIGQHLWYLLETWLPGISAAVYWPVFLLIAFAYMIGMVPWPKAVKPAARLFKVIGSYYL 81 FYIG + W L+T S Y + L ++AY+I V + IGSY+ Sbjct: 2 FYIGWNGWVWLQTSFGWQSWGYYALLVALFSYAYVI------VQVFKFLPFLRTIGSYWF 55 Query: 82 ACMEFAIIVLPLTDLLYWVLGLMGVQRTAFTAEAGXXXXXXXXXFLIWGSRNAWSTVVRN 141 A M++A+I+LPL D+ ++L L+ V R G +G+ NA+S +VR Sbjct: 56 AVMQYALILLPLADIAVFILQLVAVPRDTAIIWTGIVTLIMFLFIFGYGTFNAYSPIVRK 115 Query: 142 HPIQIGKSAGTSAPLTIAVASDLHLGNIVGNRHLRRMVAEMNRMQPDLILLAGDVLDDSI 201 + I I K A L IA+ASD+H G + G HL+R+V +N M+PD+ILL GD++DD Sbjct: 116 YEIHIPKEAKGRKTLRIAMASDMHFGKLSGVSHLKRLVHHVNEMKPDIILLPGDIIDDHP 175 Query: 202 EPFIRNGMCEEMRKLQARHGVYAVLGNHEYYGGGXXXXXTELMDSIGIKVLQDEVVE-AA 260 FI+ M + M+++QA GVY VLGNHEYYG E M I I ++ DEV++ Sbjct: 176 GEFIKKNMGQVMKQMQAPLGVYGVLGNHEYYGRAIPEFLQE-MKKIDIHIMLDEVIKMEE 234 Query: 261 GLYIVGRKDKTAESTEAAGRKSVEALLDGLDLSRPVIMMDHQPTGFGIAAQAGVDVLLSG 320 G Y+VGR DKT R+S E L+ +D S PVI MDHQP AA++GVD+LLSG Sbjct: 235 GFYLVGRTDKTERD-----RQSFEELMSTVDKSLPVIAMDHQPFELKQAAESGVDLLLSG 289 Query: 321 HTHRGQIAPNHWITRRLFELDWGYLRKDKLHVVVSSGYGTWG 362 HTHRGQ+APNH ITRR++ELDWGYL+K+ H +VSSG+G WG Sbjct: 290 HTHRGQMAPNHLITRRMYELDWGYLQKETFHAIVSSGFGFWG 331 >C3AIJ3.1 C3AIJ3_BACMY Metallophosphoesterase Length = 350 Score = 276 bits (705), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 151/342 (44%), Positives = 205/342 (59%), Gaps = 13/342 (3%) Query: 22 FYIGQHLWYLLETWLPGISAAVYWPVFLLIAFAYMIGMVPWPKAVKPAARLFKVIGSYYL 81 FYIG + W L+T S Y + L ++AY+I V + IGSY+ Sbjct: 2 FYIGWNGWVWLQTSFGWQSWGYYALLVALFSYAYVI------VQVFKFLPFLRTIGSYWF 55 Query: 82 ACMEFAIIVLPLTDLLYWVLGLMGVQRTAFTAEAGXXXXXXXXXFLIWGSRNAWSTVVRN 141 A M++A+I+LPL D+ ++L L+ V R G +G+ NA+S +VR Sbjct: 56 AVMQYALILLPLADIAVFILQLVAVPRDTAIIWTGIVTLIMFLFIFGYGTFNAYSPIVRK 115 Query: 142 HPIQIGKSAGTSAPLTIAVASDLHLGNIVGNRHLRRMVAEMNRMQPDLILLAGDVLDDSI 201 + I I K A L IA+ASD+H G + G HL+R+V +N M+PD+ILL GD++DD Sbjct: 116 YEIHIPKEAKGRKTLRIAMASDMHFGKLSGVSHLKRLVHHVNEMKPDIILLPGDIIDDHP 175 Query: 202 EPFIRNGMCEEMRKLQARHGVYAVLGNHEYYGGGXXXXXTELMDSIGIKVLQDEVVE-AA 260 FI+ M + M+++QA GVY VLGNHEYYG E M I I ++ DEV++ Sbjct: 176 GEFIKKNMGQVMKQMQAPLGVYGVLGNHEYYGRAIPEFLQE-MKKIDIHIMLDEVIKMEE 234 Query: 261 GLYIVGRKDKTAESTEAAGRKSVEALLDGLDLSRPVIMMDHQPTGFGIAAQAGVDVLLSG 320 G Y+VGR DKT R+S E L+ +D S PVI MDHQP AA++GVD+LLSG Sbjct: 235 GFYLVGRTDKTERD-----RQSFEELMSTVDKSLPVIAMDHQPFELKQAAESGVDLLLSG 289 Query: 321 HTHRGQIAPNHWITRRLFELDWGYLRKDKLHVVVSSGYGTWG 362 HTHRGQ+APNH ITRR++ELDWGYL+K+ H +VSSG+G WG Sbjct: 290 HTHRGQMAPNHLITRRMYELDWGYLQKETFHAIVSSGFGFWG 331 >C3BH22.1 C3BH22_9BACI Metallophosphoesterase Length = 350 Score = 275 bits (704), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 150/342 (43%), Positives = 206/342 (60%), Gaps = 13/342 (3%) Query: 22 FYIGQHLWYLLETWLPGISAAVYWPVFLLIAFAYMIGMVPWPKAVKPAARLFKVIGSYYL 81 FYIG + W L+T S Y + L ++AY+I V + IGSY+ Sbjct: 2 FYIGWNGWVWLQTSFGWQSWGYYALLVALFSYAYVI------VQVFKFLPFLRTIGSYWF 55 Query: 82 ACMEFAIIVLPLTDLLYWVLGLMGVQRTAFTAEAGXXXXXXXXXFLIWGSRNAWSTVVRN 141 A M++A+I+LPL D+ ++L L+ V R G +G+ NA+S +VR Sbjct: 56 AVMQYALILLPLADIAVFILQLVAVPRDTAIIWTGIVTLIMFLFIFGYGTFNAYSPIVRK 115 Query: 142 HPIQIGKSAGTSAPLTIAVASDLHLGNIVGNRHLRRMVAEMNRMQPDLILLAGDVLDDSI 201 + I I K A L IA+ASD+H G + G HL+R+V +N M+PD+ILL GD++DD Sbjct: 116 YEIHIPKEAKRRKTLRIAMASDMHFGKLSGVSHLKRLVHHVNEMKPDIILLPGDIIDDHP 175 Query: 202 EPFIRNGMCEEMRKLQARHGVYAVLGNHEYYGGGXXXXXTELMDSIGIKVLQDEVVE-AA 260 FI+ M + M+++QA GVY VLGNHEYYG E M I I ++ DEV++ Sbjct: 176 GEFIKKNMGQVMKQMQAPLGVYGVLGNHEYYGRAIPEFLQE-MKKIDIHIMLDEVIKMEE 234 Query: 261 GLYIVGRKDKTAESTEAAGRKSVEALLDGLDLSRPVIMMDHQPTGFGIAAQAGVDVLLSG 320 G Y+VGR+DKT R+S E L+ +D S PVI +DHQP AA++GVD+LLSG Sbjct: 235 GFYLVGRRDKTERD-----RQSFEELMSTVDKSLPVIAIDHQPFELKQAAESGVDLLLSG 289 Query: 321 HTHRGQIAPNHWITRRLFELDWGYLRKDKLHVVVSSGYGTWG 362 HTHRGQ+APNH ITRR++ELDWGYL+K+ H +VSSG+G WG Sbjct: 290 HTHRGQMAPNHLITRRMYELDWGYLQKETFHAIVSSGFGFWG 331 >C2W4Y6.1 C2W4Y6_BACCE Metallophosphoesterase Length = 349 Score = 263 bits (672), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 145/342 (42%), Positives = 201/342 (58%), Gaps = 14/342 (4%) Query: 22 FYIGQHLWYLLETWLPGISAAVYWPVFLLIAFAYMIGMVPWPKAVKPAARLFKVIGSYYL 81 FYIG + W L+ + Y + L ++AY+I V + IGSY+ Sbjct: 2 FYIGWNGWVWLQVTFGWEAWGYYAALVGLFSYAYLI------VQVFKFLPFLRTIGSYWF 55 Query: 82 ACMEFAIIVLPLTDLLYWVLGLMGVQRTAFTAEAGXXXXXXXXXFLIWGSRNAWSTVVRN 141 A +++A+I++PL D+ ++L L T G +G+ NA+S VVR Sbjct: 56 AVIQYALILVPLADIAVFILQLFAPIDTVIMW-TGIVTIGVFLFVFGYGTFNAYSPVVRK 114 Query: 142 HPIQIGKSAGTSAPLTIAVASDLHLGNIVGNRHLRRMVAEMNRMQPDLILLAGDVLDDSI 201 + I + K A L IA+ASD+H G + G HL+R+V +N M+PD+ILL GD++DD Sbjct: 115 YEIHLPKEAKGRKTLRIAMASDMHFGKLSGTSHLKRLVHHVNEMKPDIILLPGDIIDDHP 174 Query: 202 EPFIRNGMCEEMRKLQARHGVYAVLGNHEYYGGGXXXXXTELMDSIGIKVLQDEVVEAA- 260 FI+ M + M+++QA G Y VLGNHEYYG E MD I I ++ DEV++ Sbjct: 175 GEFIKKNMGQVMKQMQAPLGTYGVLGNHEYYGRAIPEFLQE-MDKIDIHIMLDEVIKVED 233 Query: 261 GLYIVGRKDKTAESTEAAGRKSVEALLDGLDLSRPVIMMDHQPTGFGIAAQAGVDVLLSG 320 Y+VGR+DKT RKS E L+ +D PVI MDHQP AA++GVD+LLSG Sbjct: 234 SFYLVGRRDKTERD-----RKSFEELMSTVDKELPVIAMDHQPFELKQAAESGVDLLLSG 288 Query: 321 HTHRGQIAPNHWITRRLFELDWGYLRKDKLHVVVSSGYGTWG 362 HTHRGQ+APNH ITRR++ELDWGYL+K+ H +VSSG+G WG Sbjct: 289 HTHRGQMAPNHLITRRMYELDWGYLQKETFHAIVSSGFGFWG 330 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.139 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,058,447,069 Number of extensions: 82196277 Number of successful extensions: 215590 Number of sequences better than 1.0e-05: 775 Number of HSP's gapped: 214203 Number of HSP's successfully gapped: 775 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)