BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0578.0001.p (395 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B4BD52.1 B4BD52_CLOTM NUDIX hydrolase 376 e-102 A3DK42.1 A3DK42_CLOTH NUDIX hydrolase 376 e-102 C0Z932.1 C0Z932_BREBN Putative uncharacterized protein 349 2e-94 B1DCF8.1 B1DCF8_9BACL NUDIX hydrolase 322 6e-86 B3K8L7.1 B3K8L7_9BACI NUDIX hydrolase 317 2e-84 >B4BD52.1 B4BD52_CLOTM NUDIX hydrolase Length = 317 Score = 376 bits (966), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/297 (58%), Positives = 226/297 (76%) Query: 95 MPKKPVTDRNGLTEEQFLQAYDAGMYERPSVTVDMLIFTVMEQEQDNYRKLSEKSLQLLM 154 M + ++ GLTEE+FL++YDA YERPSVTVDMLIFTV ++++NYRKL EK L+LLM Sbjct: 15 MSESKKVNKQGLTEEEFLESYDASKYERPSVTVDMLIFTVTNEKKENYRKLPEKVLRLLM 74 Query: 155 IQRGEHPYLGQWALPGGFVGVDESLEDAALRELYSETNIDNVYMEQLYTWGDVGRDPRMR 214 I+R +HPY+GQWALPGGF+ +DESLE+ ALREL ETNIDN+YMEQLYTWGDV RDPR R Sbjct: 75 IKRADHPYIGQWALPGGFIKMDESLEEGALRELKEETNIDNIYMEQLYTWGDVNRDPRTR 134 Query: 215 VISSSYIALVDRKTLDVQXXXXXXXXXWFEVTYDVLESGRRELAEGYMEEQKIRITLQKE 274 +IS SY+ALVD L+++ WF V+ ++ + + +GY+ ++ + L E Sbjct: 135 IISVSYMALVDSSNLNIKASDDADDAKWFTVSCNLYQEQKTLTEKGYILQRLYNLRLSDE 194 Query: 275 DVELTGVVTITETVQGHVRRVRREIAENHGFAFDHLKMIQYAIERLRGKVEYTDIIFNLM 334 + L+ V+ + +TV+G V RV REI E+HG AFDH K+I+YA+ERLR K+EYTDI FNLM Sbjct: 195 EDNLSAVIKVVKTVEGRVSRVEREIVESHGIAFDHAKIIEYAVERLRNKIEYTDIAFNLM 254 Query: 335 PPLFTLSELQRVYEIILGRELLAPAFRRKIADSVTETEEFTKDAGHRPSKLYRYRAD 391 P FTL+ELQ+VYE+ILGRELL FRRKIA+ V ET E+TKDAGHRPSKL+R+ + Sbjct: 255 PERFTLTELQQVYEVILGRELLKANFRRKIANMVIETNEYTKDAGHRPSKLFRFNPN 311 >A3DK42.1 A3DK42_CLOTH NUDIX hydrolase Length = 303 Score = 376 bits (965), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/297 (58%), Positives = 226/297 (76%) Query: 95 MPKKPVTDRNGLTEEQFLQAYDAGMYERPSVTVDMLIFTVMEQEQDNYRKLSEKSLQLLM 154 M + ++ GLTEE+FL++YDA YERPSVTVDMLIFTV ++++NYRKL EK L+LLM Sbjct: 1 MSESKKVNKQGLTEEEFLESYDASKYERPSVTVDMLIFTVTNEKKENYRKLPEKVLRLLM 60 Query: 155 IQRGEHPYLGQWALPGGFVGVDESLEDAALRELYSETNIDNVYMEQLYTWGDVGRDPRMR 214 I+R +HPY+GQWALPGGF+ +DESLE+ ALREL ETNIDN+YMEQLYTWGDV RDPR R Sbjct: 61 IKRADHPYIGQWALPGGFIKMDESLEEGALRELKEETNIDNIYMEQLYTWGDVNRDPRTR 120 Query: 215 VISSSYIALVDRKTLDVQXXXXXXXXXWFEVTYDVLESGRRELAEGYMEEQKIRITLQKE 274 +IS SY+ALVD L+++ WF V+ ++ + + +GY+ ++ + L E Sbjct: 121 IISVSYMALVDSSNLNIKASDDADDAKWFTVSCNLYQEQKTLTEKGYILQRLYNLRLSDE 180 Query: 275 DVELTGVVTITETVQGHVRRVRREIAENHGFAFDHLKMIQYAIERLRGKVEYTDIIFNLM 334 + L+ V+ + +TV+G V RV REI E+HG AFDH K+I+YA+ERLR K+EYTDI FNLM Sbjct: 181 EDNLSAVIKVVKTVEGRVSRVEREIVESHGIAFDHAKIIEYAVERLRNKIEYTDIAFNLM 240 Query: 335 PPLFTLSELQRVYEIILGRELLAPAFRRKIADSVTETEEFTKDAGHRPSKLYRYRAD 391 P FTL+ELQ+VYE+ILGRELL FRRKIA+ V ET E+TKDAGHRPSKL+R+ + Sbjct: 241 PERFTLTELQQVYEVILGRELLKANFRRKIANMVIETNEYTKDAGHRPSKLFRFNPN 297 >C0Z932.1 C0Z932_BREBN Putative uncharacterized protein Length = 302 Score = 349 bits (896), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 165/297 (55%), Positives = 219/297 (73%) Query: 95 MPKKPVTDRNGLTEEQFLQAYDAGMYERPSVTVDMLIFTVMEQEQDNYRKLSEKSLQLLM 154 M KP ++ G TE+++L+AYD +ERPSVTVDML+FTVM++ ++NYRKLS KSL++L+ Sbjct: 1 MKDKPSVNKQGQTEQEYLEAYDVSQFERPSVTVDMLVFTVMDELEENYRKLSPKSLKILL 60 Query: 155 IQRGEHPYLGQWALPGGFVGVDESLEDAALRELYSETNIDNVYMEQLYTWGDVGRDPRMR 214 ++RGEHPY+GQWALPGGFV ESLE+AA REL +ETN+D++Y+EQLYTWGD GRDPR Sbjct: 61 VKRGEHPYIGQWALPGGFVTPGESLEEAARRELRTETNVDDIYLEQLYTWGDAGRDPRTW 120 Query: 215 VISSSYIALVDRKTLDVQXXXXXXXXXWFEVTYDVLESGRRELAEGYMEEQKIRITLQKE 274 VIS+SY+ALVD +L +Q W+ + L+ + +G + E+ + + L E Sbjct: 121 VISTSYMALVDSSSLQLQAGDDAEEAEWYRIEDRWLKETKTATLDGSITEKWLELRLVHE 180 Query: 275 DVELTGVVTITETVQGHVRRVRREIAENHGFAFDHLKMIQYAIERLRGKVEYTDIIFNLM 334 +L+ + IT+TV G R REI E + AFDH K+IQYA+ERLR K+EYTDI F LM Sbjct: 181 REQLSATIKITKTVTGRTVRETREIVETNNLAFDHAKIIQYALERLRNKIEYTDIAFALM 240 Query: 335 PPLFTLSELQRVYEIILGRELLAPAFRRKIADSVTETEEFTKDAGHRPSKLYRYRAD 391 P LFTLS+LQ+VYE+ILGRELLA AFRRK+AD V ET ++ K AGHRPSK +R+ + Sbjct: 241 PELFTLSDLQQVYEVILGRELLAAAFRRKVADKVVETNQYRKHAGHRPSKYFRFNPE 297 >B1DCF8.1 B1DCF8_9BACL NUDIX hydrolase Length = 297 Score = 322 bits (824), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 156/286 (54%), Positives = 202/286 (70%) Query: 102 DRNGLTEEQFLQAYDAGMYERPSVTVDMLIFTVMEQEQDNYRKLSEKSLQLLMIQRGEHP 161 D NGLTE F++ Y G YERPSV DM+IFTV E++NYRKL EK L+LL+IQRG HP Sbjct: 5 DPNGLTERDFMEQYKPGDYERPSVAADMVIFTVTHTEENNYRKLPEKELRLLLIQRGGHP 64 Query: 162 YLGQWALPGGFVGVDESLEDAALRELYSETNIDNVYMEQLYTWGDVGRDPRMRVISSSYI 221 +LGQWALPGGFV E+ E+AA+REL+ ET +DNVY+EQLYT+ D+GRDPR V+S SY+ Sbjct: 65 FLGQWALPGGFVRPHETTEEAAVRELHEETGVDNVYLEQLYTFSDIGRDPRTWVMSCSYM 124 Query: 222 ALVDRKTLDVQXXXXXXXXXWFEVTYDVLESGRRELAEGYMEEQKIRITLQKEDVELTGV 281 ALV+ K L+++ WF+ +Y ++ + + GY + + L + EL + Sbjct: 125 ALVNSKELELKAGDDAANAAWFKASYRLIREQKEFVEGGYARTLQYELLLSSDYAELKAM 184 Query: 282 VTITETVQGHVRRVRREIAENHGFAFDHLKMIQYAIERLRGKVEYTDIIFNLMPPLFTLS 341 V T+TV + I N G AFDH K+I YAIERLRGKV YTDI +LMP LFTL+ Sbjct: 185 VERTDTVTEAATKTAYSIVSNDGLAFDHAKIIAYAIERLRGKVNYTDIALHLMPKLFTLT 244 Query: 342 ELQRVYEIILGRELLAPAFRRKIADSVTETEEFTKDAGHRPSKLYR 387 ELQ+VYE+IL +ELL AFRRKIAD VTET+ +T+++GHRPS+LYR Sbjct: 245 ELQQVYEVILDKELLKAAFRRKIADLVTETDHYTENSGHRPSRLYR 290 >B3K8L7.1 B3K8L7_9BACI NUDIX hydrolase Length = 294 Score = 317 bits (811), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 154/286 (53%), Positives = 207/286 (72%), Gaps = 1/286 (0%) Query: 102 DRNGLTEEQFLQAYDAGMYERPSVTVDMLIFTVMEQEQDNYRKLSEKSLQLLMIQRGEHP 161 DRNGLTE +FL+ Y AG YERPSV DM+IFTV ++E D+YRKL EK L++L+I+RG HP Sbjct: 5 DRNGLTEREFLEQYRAGDYERPSVAADMVIFTVTDEEADSYRKLPEKELRILLIRRGGHP 64 Query: 162 YLGQWALPGGFVGVDESLEDAALRELYSETNIDNVYMEQLYTWGDVGRDPRMRVISSSYI 221 +LG+WALPGGFV E+ E AA REL ET++D+VY+EQLYT+ D+GRDPR V+S SY+ Sbjct: 65 FLGKWALPGGFVQPSETTEQAAARELREETSVDDVYLEQLYTFSDIGRDPRTWVMSCSYM 124 Query: 222 ALVDRKTLDVQXXXXXXXXXWFEVTYDVLESGRRELAEGYMEEQKIRITLQKEDVELTGV 281 AL++ L+++ WF+V+Y +L + + +GY++ + + L E+ EL+ V Sbjct: 125 ALINSDKLELRAGDDAADASWFKVSYRLLREQKELMEDGYVKTLEYELKLSGEE-ELSAV 183 Query: 282 VTITETVQGHVRRVRREIAENHGFAFDHLKMIQYAIERLRGKVEYTDIIFNLMPPLFTLS 341 V T TV+ +I N G AFDH K+I AIERLRGKV YTDI +LMP LFTL+ Sbjct: 184 VARTLTVKTTSTSTEYKIVSNDGLAFDHAKIIACAIERLRGKVNYTDIALHLMPKLFTLT 243 Query: 342 ELQRVYEIILGRELLAPAFRRKIADSVTETEEFTKDAGHRPSKLYR 387 ELQ+VYE+I+ +ELL AFRRK+AD V ET+ +T++AGHRPS+LYR Sbjct: 244 ELQQVYEVIMDKELLKAAFRRKVADLVAETDHYTENAGHRPSRLYR 289 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,818,468,011 Number of extensions: 84984196 Number of successful extensions: 164557 Number of sequences better than 1.0e-05: 477 Number of HSP's gapped: 164223 Number of HSP's successfully gapped: 648 Length of query: 395 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 257 Effective length of database: 1,760,596,133 Effective search space: 452473206181 Effective search space used: 452473206181 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)