BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0034.p (291 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3MUH7.1 Q3MUH7_9BACL Xyloglucanase 409 e-112 C1W388.1 C1W388_9MICO Cellulose binding protein with BNR/Asp-box... 322 3e-86 B3PKK9.1 B3PKK9_CELJU Endo-1,4-beta-glucanase/xyloglucanase, put... 317 1e-84 A9AWC8.1 A9AWC8_HERA2 Cellulose-binding family II 312 2e-83 B5HGN4.1 B5HGN4_STRPR Secreted cellulase 310 1e-82 >Q3MUH7.1 Q3MUH7_9BACL Xyloglucanase Length = 1027 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/282 (70%), Positives = 232/282 (82%), Gaps = 4/282 (1%) Query: 8 RKLGLVVLLAAMTSSLFTAFPAITRAAASEAYNWKSVVTGAGGGFVPGIIFNQSEPDLIY 67 +KL + L A+ + F A P +T AA SE Y WK+VVTGAGGGFVPGIIFN+SE DLIY Sbjct: 7 KKLWMASLAVALAAGSFAALPEMTSAAPSEPYTWKNVVTGAGGGFVPGIIFNESEKDLIY 66 Query: 68 ARTDIGGAYRWNQATGSWVSISDAVGWVDWNKNGVDALATDPIDPDKVYMATGTYTNSWD 127 ARTDIGGAYRWN A SW+ ++D VGW DWNKNGVDALATDP+DPD+VY+A GTYTNSWD Sbjct: 67 ARTDIGGAYRWNPANESWIPLTDFVGWDDWNKNGVDALATDPVDPDRVYLAVGTYTNSWD 126 Query: 128 -DNGQIMRSSDRGNTWQSTPLPFKVGGNMPGRSAGERLVIDPNKNSILFFGARSGNGLWK 186 +NG I+RS+DRG+TWQ+T LPFKVGGNMPGRS GERLV+DPN N IL+FGARSGNGLW+ Sbjct: 127 KNNGAILRSTDRGDTWQTTTLPFKVGGNMPGRSMGERLVVDPNDNRILYFGARSGNGLWR 186 Query: 187 STDSGVTWSKVSSFPNAGTYIQNPTLEYGNDLVGLSWITFDQSTGTLGSATQTIYVGVAD 246 S+D G TWSKV+SFPN GTY+Q+P EYG+D+VGL+WITFD+S+G +G ATQTIYVGVAD Sbjct: 187 SSDYGATWSKVTSFPNPGTYVQDPANEYGSDIVGLAWITFDKSSGQVGQATQTIYVGVAD 246 Query: 247 TASSVYRSTDGGATWTAIPGQPTGXXXPAASRGAFFYRGSIY 288 TA S+YRSTDGGATWTA+PGQPTG G GS+Y Sbjct: 247 TAQSIYRSTDGGATWTAVPGQPTGYL---PHHGVLDADGSLY 285 >C1W388.1 C1W388_9MICO Cellulose binding protein with BNR/Asp-box repeat Length = 928 Score = 322 bits (824), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 159/278 (57%), Positives = 199/278 (71%), Gaps = 5/278 (1%) Query: 14 VLLAAMTSSLFTAFPAITRAAASEAYNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIG 73 V LAA S L TA A + + Y+W +V GGGFVPGIIFNQSEP L+YARTDIG Sbjct: 14 VGLAATASPLATAPAAYSNGDSPSGYSWSNVEI-VGGGFVPGIIFNQSEPGLVYARTDIG 72 Query: 74 GAYRWNQATGSWVSISDAVGWVDWNKNGVDALATDPIDPDKVYMATGTYTNSWD-DNGQI 132 GAYR ++ +G W ++D +GW W+ +G+ +LATDP+DPD+VY+A GTYT WD NG I Sbjct: 73 GAYRLDEESGRWKPLNDDLGWDQWSHSGILSLATDPVDPDRVYLAAGTYTTDWDPQNGAI 132 Query: 133 MRSSDRGNTWQSTPLPFKVGGNMPGRSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGV 192 +RS+DRGNTW+ T LPFK+GGNMPGR GERL IDPN N IL+ G SGNGLWKSTD G Sbjct: 133 LRSTDRGNTWEKTMLPFKIGGNMPGRGMGERLAIDPNNNKILYLGTESGNGLWKSTDYGE 192 Query: 193 TWSKVSSFPNAGTYIQNPTLEYGNDLVGLSWITFDQSTGTLGSATQTIYVGVADTASSVY 252 TW+KV+ FPNAG Y+Q+P+ +Y D G+ W+TFD ++ G TQTIYVGVAD ++VY Sbjct: 193 TWNKVTEFPNAGNYVQDPSYDYTADNQGVVWVTFDPTSANAGQTTQTIYVGVADKDNTVY 252 Query: 253 RSTDGGATWTAIPGQPTGXXXPAASRGAFFYRGSIYYV 290 R+TDGG+TW IPGQPTG A +G + G Y+ Sbjct: 253 RTTDGGSTWERIPGQPTGY---LAQKGVLDHEGQQLYI 287 >B3PKK9.1 B3PKK9_CELJU Endo-1,4-beta-glucanase/xyloglucanase, putative, gly74A EC=3.2.1.- Length = 1017 Score = 317 bits (811), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 161/273 (58%), Positives = 201/273 (73%), Gaps = 9/273 (3%) Query: 1 MVTRKRIRKLGLVVLLAAMTSSLFTAFPAITRAAASEAYNWKSVVTGAGGGFVPGIIFNQ 60 ++ R L + + A+ S+ A + AA SE Y WK+V GGGFVPGIIFNQ Sbjct: 6 LLKRSVQTSLAMGAFMGALWGSI-----APSWAAPSENYTWKNVRI-DGGGFVPGIIFNQ 59 Query: 61 SEPDLIYARTDIGGAYRWNQATGSWVSISDAVGWVDWNKNGVDALATDPIDPDKVYMATG 120 E DLIYARTDIGGAYRWN AT SW+ + D VGW +W NGV +LATD DP++VY A G Sbjct: 60 KEADLIYARTDIGGAYRWNSATSSWIPLLDWVGWDNWGWNGVMSLATDAADPNRVYAAVG 119 Query: 121 TYTNSWD-DNGQIMRSSDRGNTWQSTPLPFKVGGNMPGRSAGERLVIDPNKNSILFFGAR 179 YTN+WD +NG I+RS+DRGNTWQ+TPLPFKVGGNMPGR GERL IDPN+NSI+++GA Sbjct: 120 MYTNTWDPNNGAILRSTDRGNTWQATPLPFKVGGNMPGRGMGERLAIDPNRNSIIYYGAE 179 Query: 180 SGNGLWKSTDSGVTWSKVSSFPNAGTYIQNPT--LEYGNDLVGLSWITFDQSTGTLGSAT 237 GNGLW+STD G TW+KVSSF N G Y Q+P +Y N + G+ W+TFD ++G+ G+ + Sbjct: 180 GGNGLWRSTDYGATWAKVSSFTNGGNYAQDPNDPNDYLNKIQGVVWVTFDPASGSAGNTS 239 Query: 238 QTIYVGVADTASSVYRSTDGGATWTAIPGQPTG 270 Q IYVGVADT +++YRSTDGG TW+ + GQPTG Sbjct: 240 QVIYVGVADTQNAIYRSTDGGTTWSRLAGQPTG 272 >A9AWC8.1 A9AWC8_HERA2 Cellulose-binding family II Length = 942 Score = 312 bits (799), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 160/283 (56%), Positives = 203/283 (71%), Gaps = 12/283 (4%) Query: 5 KRIRKLGLVVLLAAMTSSLFTAFPAITRAAASEAYNWKSVVTGAGGGFVPGIIFNQSEPD 64 + ++ VV+L A A P +RAA+++ YNW +V GGGFVPGII+N +E D Sbjct: 2 RMLKMAAAVVVLFAGVIQAMVAVPQ-SRAASTQPYNWSNVEI-VGGGFVPGIIYNPTERD 59 Query: 65 LIYARTDIGGAYRWNQATGSWVSISDAVGWVDWNKNGVDALATDPIDPDKVYMATGTYTN 124 L+YARTDIGGAYRWN T W+ ++D +G DWN G+D+LATDP+DP+++Y+A GTYTN Sbjct: 60 LVYARTDIGGAYRWNPTTKRWIPLTDWIGATDWNWTGIDSLATDPVDPNRLYLAAGTYTN 119 Query: 125 SWDD-NGQIMRSSDRGNTWQSTPLPFKVGGNMPGRSAGERLVIDPNKNSILFFGARSGNG 183 W NG I+RS+++GN+W T LPFK+GGNMPGRS GERL IDPNKNS+L+ G R G G Sbjct: 120 DWTSANGAILRSTNKGNSWARTDLPFKLGGNMPGRSMGERLAIDPNKNSVLYLGTR-GQG 178 Query: 184 LWKSTDSGVTWSKVSSFPNAGTYIQNPTLEYGNDLVGLSWITFDQSTGTLGSATQTIYVG 243 LW+STD G TW++V+SFP G Y T Y ND +G+ W+TFD TGT GSA+QTIYVG Sbjct: 179 LWRSTDYGATWAQVTSFPAIGNY----TDPYFNDRIGVVWVTFDPRTGTSGSASQTIYVG 234 Query: 244 VADTASSVYRSTDGGATWTAIPGQPTGXXXPA----ASRGAFF 282 VADT +S+YRSTDGGATW A+PGQPT P AS G + Sbjct: 235 VADTTTSIYRSTDGGATWAALPGQPTAGYLPHHATLASNGMLY 277 >B5HGN4.1 B5HGN4_STRPR Secreted cellulase Length = 910 Score = 310 bits (793), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 155/239 (64%), Positives = 186/239 (77%), Gaps = 4/239 (1%) Query: 35 ASEAYNWKSVVTGAGGGFVPGIIFNQSEPDLIYARTDIGGAYRWNQATGSWVSISDAVGW 94 A++ Y WK+V GGGFVPGI+FN+SE DL YARTDIGGAYRWN+A W + D V W Sbjct: 61 AADTYTWKNVRI-DGGGFVPGIVFNRSEKDLAYARTDIGGAYRWNEAGKEWTPLLDWVDW 119 Query: 95 VDWNKNGVDALATDPIDPDKVYMATGTYTNSWD-DNGQIMRSSDRGNTWQSTPLPFKVGG 153 W GV +LA+D +DPDKVY A GTYTNSWD NG ++RSSDRG +WQ+T LPFK+GG Sbjct: 120 DRWGWTGVVSLASDTVDPDKVYAAVGTYTNSWDPTNGAVLRSSDRGASWQATTLPFKLGG 179 Query: 154 NMPGRSAGERLVIDPNKNSILFFGARSGNGLWKSTDSGVTWSKVSSFPNAGTYIQNPTLE 213 NMPGR GERL +DPNKNS+L+ GA SG+GLW+STDSGVTWSKV++FPN G Y Q+PT Sbjct: 180 NMPGRGMGERLAVDPNKNSVLYLGAPSGHGLWRSTDSGVTWSKVTAFPNPGNYAQDPTDT 239 Query: 214 --YGNDLVGLSWITFDQSTGTLGSATQTIYVGVADTASSVYRSTDGGATWTAIPGQPTG 270 Y ND G+ W+TFD+ +G+ GSATQ IYVGVAD ++VYRSTDGGATW+ I GQPTG Sbjct: 240 SGYANDNQGVVWVTFDERSGSAGSATQHIYVGVADKENTVYRSTDGGATWSRIAGQPTG 298 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.132 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,959,373,780 Number of extensions: 89980658 Number of successful extensions: 233498 Number of sequences better than 1.0e-05: 207 Number of HSP's gapped: 232793 Number of HSP's successfully gapped: 249 Length of query: 291 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 157 Effective length of database: 1,798,280,193 Effective search space: 282329990301 Effective search space used: 282329990301 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)