BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0033.p (796 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q3MUH7.1 Q3MUH7_9BACL Xyloglucanase 629 e-178 C1QVW1.1 C1QVW1_9ACTO Cellobiohydrolase A (1,4-beta-cellobiosida... 459 e-127 C4E636.1 C4E636_STRRS Putative cellulose-binding protein 456 e-126 A3DFA0.1 A3DFA0_CLOTH Cellulosome enzyme, dockerin type I 453 e-125 B5I4S8.1 B5I4S8_9ACTO Endo-1,4-beta-glucanase 450 e-124 >Q3MUH7.1 Q3MUH7_9BACL Xyloglucanase Length = 1027 Score = 629 bits (1622), Expect = e-178, Method: Compositional matrix adjust. Identities = 320/554 (57%), Positives = 393/554 (70%), Gaps = 23/554 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VDAQ P T+MV++LN+WWPD ++FRS +GG TW+RIW++ YP R +++ +I+AAPWL Sbjct: 334 VDAQEPGTLMVATLNSWWPDAILFRSKDGGTTWTRIWEFDGYPNRKFRYTQNISAAPWLT 393 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G T + + SPKLGWM+GDLEIDPF+SDRMMYGTGATIY TNNLT+WD+ K++I+VM Sbjct: 394 FGTTPAPP-EVSPKLGWMIGDLEIDPFDSDRMMYGTGATIYGTNNLTNWDNNEKIDISVM 452 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 AKGVEE AVL L+SPPSG AHL++ LGDV+GFR++DL Q P K +SP++A+T +D+AE Sbjct: 453 AKGVEEMAVLDLVSPPSG-AHLVSGLGDVNGFRHDDLDQPPAKMFSSPNYASTESLDFAE 511 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 LNP+ +VRVG AD P+ KSIG+S+DGG NWY N+EP+ TAG G VA+S+ G+ Sbjct: 512 LNPSTMVRVGKADYAADPNAKSIGLSSDGGTNWYKANAEPAG----TAGGGTVAISSDGS 567 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 ++WSTSD GV+YS T GNSW S+G+PA AKV SDRVNPNKFY F AG Y+ Sbjct: 568 KLVWSTSDKGVHYSSTGGNSWTASTGIPAQAKVISDRVNPNKFYGFAAGKIYVSVNGGVS 627 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 GL P + A+PG+EG++WFAGGN G YGLWHSTDSG SF Sbjct: 628 FSQTA--------AAGL-PVDGNADLDAVPGVEGELWFAGGNEDGGPYGLWHSTDSGASF 678 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 KL+NVEEAD IGFG AAPG+N ALY VA+IDG RG FRSDD G+SWVRINDDAHQYA+ Sbjct: 679 AKLSNVEEADSIGFGKAAPGRNSAALYAVAQIDGTRGFFRSDDGGASWVRINDDAHQYAR 738 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYADPVNXX-------XXXXXXXXXXXXFDKKTANQ 473 + ITGDPR+YGRVYLGTNGRG LYADPV FD Q Sbjct: 739 V-TTITGDPRIYGRVYLGTNGRGILYADPVGGNNGGETPPVSHSGISPQSTEFDLNADRQ 797 Query: 474 ADIAVTLTLNGTTLSSIKNGTTALSAGADYTVSGTNVTIKKEYLATQATGITRLSFNFSA 533 ADI V LTLNG TL+SI+NG L G+DYT+SG+ V + K YLAT + G+ L F FSA Sbjct: 798 ADIPVALTLNGNTLASIRNGNHVLVQGSDYTMSGSQVFLSKTYLATLSKGVQSLVFRFSA 857 Query: 534 GAAKTLTIAVSDTT 547 G TL+I V DTT Sbjct: 858 GNDATLSITVKDTT 871 Score = 254 bits (649), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 130/254 (51%), Positives = 171/254 (67%), Gaps = 3/254 (1%) Query: 545 DTTGTANSIISPV-TXXXXXXXXXADIPVTLTLNGNTLSSIKNGTAVLTAGTDYTVSGTT 603 +T ++S ISP T ADIPV LTLNGNTL+SI+NG VL G+DYT+SG+ Sbjct: 774 ETPPVSHSGISPQSTEFDLNADRQADIPVALTLNGNTLASIRNGNHVLVQGSDYTMSGSQ 833 Query: 604 VTIKKEYLAAQPVGTTTLSFSFSAGAAQTLAVTIVDT--VAPPAGALKVQMYNSGTAASA 661 V + K YLA G +L F FSAG TL++T+ DT V P G+++++MYN T+A+A Sbjct: 834 VFLSKTYLATLSKGVQSLVFRFSAGNDATLSITVKDTTQVPLPEGSIRIEMYNGTTSATA 893 Query: 662 NTISPKFKLVNTGTSAITLSGVKLRYYYTIDGEQTQNFFCDWSQVGSANVTGSFVKMPSA 721 N+I+PKFKL NTGT+ + L+ V +RYYYTIDGE+ NFFCDW+ GSANVTG+F +P+A Sbjct: 894 NSINPKFKLTNTGTAPLQLADVNIRYYYTIDGEKPLNFFCDWATAGSANVTGTFSALPAA 953 Query: 722 TTGADYYLELGFNXXXXXXXXXXXIDIQARVAKSDWTNYTQTGDYSFNGADTNYADWSKA 781 GAD+ LE+GF ++Q R +K+DWTN+TQT DYSF + T Y +WSK Sbjct: 954 VNGADHVLEIGFTASAGTLAAGQSTEVQVRFSKTDWTNFTQTDDYSFAASSTAYENWSKV 1013 Query: 782 PAYISGTLVWGNEP 795 Y+SGTL WG EP Sbjct: 1014 TGYVSGTLQWGIEP 1027 >C1QVW1.1 C1QVW1_9ACTO Cellobiohydrolase A (1,4-beta-cellobiosidase A) Length = 935 Score = 459 bits (1181), Expect = e-127, Method: Compositional matrix adjust. Identities = 241/449 (53%), Positives = 309/449 (68%), Gaps = 22/449 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 +D QHP+T+MV+ ++WWPD I+RST+GGATW+ W + YP R K+ +D++A+PWL+ Sbjct: 350 IDQQHPNTIMVTGYSSWWPDTFIYRSTDGGATWTNAWSYNGYPTRVDKYTLDVSASPWLS 409 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSG-GKVNIAV 119 G S + +PKLGWM L IDPFNSDRMM+GTGAT+Y T NLT+WDS KV ++V Sbjct: 410 WGNYPSPP-EETPKLGWMSEGLAIDPFNSDRMMFGTGATLYGTTNLTAWDSSTAKVKLSV 468 Query: 120 MAKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYA 179 MA+G+EETAV L+SPPSG A L++ALGDV GFR+ DLT+ P SP+W+T+T IDYA Sbjct: 469 MAQGIEETAVTDLVSPPSG-APLLSALGDVDGFRHTDLTKVPAMMYQSPNWSTSTSIDYA 527 Query: 180 ELNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASG 239 E NP VVRVG + ++ S S+D G +WY +S+PS TAG G VA++A G Sbjct: 528 EANPNDVVRVG----NGSSTVNSAAFSSDDGADWYAASSQPSG---VTAG-GTVAMAADG 579 Query: 240 NSILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAF-YA-GTFYIXXXX 297 ++ +WS + V Y+ TTG+SW S+G+PAGA+V SDRVNP KFY F YA GTFY+ Sbjct: 580 SAAVWSPTSAAVSYTTTTGSSWTASTGIPAGAQVRSDRVNPKKFYGFNYATGTFYLSTDG 639 Query: 298 XXXXXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSG 357 L + A+PG EGD+W AGG + G YGLWHSTDSG Sbjct: 640 GKTFTASAATN--------LPSGTGGAYVHAVPGTEGDLWLAGG-STSGAYGLWHSTDSG 690 Query: 358 TSFTKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQ 417 ++TKL NV++AD IGFG APG+ ALYT+A+I GVRG+FRSDD GS+W RINDD HQ Sbjct: 691 ATWTKLANVQQADNIGFGAPAPGRTNKALYTIAEIGGVRGIFRSDDDGSTWTRINDDQHQ 750 Query: 418 YAKINMAITGDPRVYGRVYLGTNGRGTLY 446 + I AITGDPRVYGRVY+GTNGRG +Y Sbjct: 751 WGNIGAAITGDPRVYGRVYVGTNGRGIVY 779 >C4E636.1 C4E636_STRRS Putative cellulose-binding protein Length = 971 Score = 456 bits (1172), Expect = e-126, Method: Compositional matrix adjust. Identities = 234/449 (52%), Positives = 290/449 (64%), Gaps = 17/449 (3%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 +D HP T+MV++ +WWPD + FRST+ GATW+RIWDW YP RT+++ MDI+++PWL Sbjct: 331 IDRLHPGTLMVATQVSWWPDVIFFRSTDAGATWTRIWDWASYPNRTFRYKMDISSSPWLT 390 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G N + +PKLGWM LEIDPF+S+RMMYGTGAT+Y T +L WD+GG+ I M Sbjct: 391 FG-ANPQPPETTPKLGWMTESLEIDPFDSNRMMYGTGATLYGTEDLLKWDTGGQFTIKPM 449 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 +G+EETAVL LISPPSG A L++ LGD+ GFR+ DL P TSP + +TT +DYAE Sbjct: 450 VRGLEETAVLDLISPPSG-APLVSGLGDIGGFRHTDLAAVPPMMFTSPVFTSTTSLDYAE 508 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 PA +VR G PS + S DGG NW+ +EP + G VA +A G+ Sbjct: 509 TKPAVMVRAGNFTDADRPSDSHVAFSTDGGANWFQ-GTEPGGINE----GGTVAAAADGS 563 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 +W+ + V+ S G SW S+G+PAGA V SDRVNP +FY F AG FY Sbjct: 564 RFVWAPKGVAVHRSTGFGTSWTASTGIPAGAVVESDRVNPARFYGFGAGRFYASTDGGAS 623 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGN-AVEGKYGLWHSTDSGTS 359 GL P + KA+PG EGDIW AGG+ A G G+WHSTD G S Sbjct: 624 FAATAAT--------GL-PATGNVKFKAVPGREGDIWLAGGDTAAGGTSGIWHSTDGGAS 674 Query: 360 FTKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYA 419 FTKL+ V A IGFG AAPG+ Y ALY VA + GV GVFRSDD G+SWVRINDD H+Y Sbjct: 675 FTKLSGVTSAVNIGFGKAAPGRAYQALYAVATVGGVNGVFRSDDTGASWVRINDDRHRYG 734 Query: 420 KINMAITGDPRVYGRVYLGTNGRGTLYAD 448 + A+TGDPRVYGRVYLGTNGRG LYAD Sbjct: 735 NMGEAVTGDPRVYGRVYLGTNGRGILYAD 763 >A3DFA0.1 A3DFA0_CLOTH Cellulosome enzyme, dockerin type I Length = 842 Score = 453 bits (1166), Expect = e-125, Method: Compositional matrix adjust. Identities = 237/449 (52%), Positives = 296/449 (65%), Gaps = 25/449 (5%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VD Q+PD +MV+S+NAWWPDE IFRST+GGATW IW+WG YPER + +DI+AAPWL+ Sbjct: 335 VDRQNPDIIMVTSMNAWWPDEYIFRSTDGGATWKNIWEWGMYPERILHYEIDISAAPWLD 394 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G T + +PKLGWM+GD+EIDPFNSDRMMY TGATIY +NLT WD GGKV I V Sbjct: 395 WG-TEKQLPEINPKLGWMIGDIEIDPFNSDRMMYVTGATIYGCDNLTDWDRGGKVKIEVK 453 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 A G+EE AVL L+SPP G A L++A+GD+ GF ++DL P K PS+++ TGIDYAE Sbjct: 454 ATGIEECAVLDLVSPPEG-APLVSAVGDLVGFVHDDLKVGPKKMHV-PSYSSGTGIDYAE 511 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 L P ++ V AD +K I S DGG NW+ P +E N + G G VAV+A Sbjct: 512 LVPNFMALVAKADLY---DVKKISFSYDGGRNWFQPPNEAPN----SVGGGSVAVAADAK 564 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 S++W+ + + GNSW V + + GA VASDRVN KFYAFY G FYI Sbjct: 565 SVIWTPENASPAVTTDNGNSWKVCTNLGMGAVVASDRVNGKKFYAFYNGKFYISTDGGLT 624 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 + Q ++ +KA+PG EG +W A A EG GLW STD G +F Sbjct: 625 F----------TDTKAPQLPKSVNKIKAVPGKEGHVWLA---AREG--GLWRSTDGGYTF 669 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 KL+NV+ A ++GFG AAPGQ+YMA+Y KID V G FRSDDAG +WVRINDD H Y Sbjct: 670 EKLSNVDTAHVVGFGKAAPGQDYMAIYITGKIDNVLGFFRSDDAGKTWVRINDDEHGYGA 729 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLYADP 449 ++ AITGDPRVYGRVY+ TNGRG +Y +P Sbjct: 730 VDTAITGDPRVYGRVYIATNGRGIVYGEP 758 >B5I4S8.1 B5I4S8_9ACTO Endo-1,4-beta-glucanase Length = 889 Score = 450 bits (1157), Expect = e-124, Method: Compositional matrix adjust. Identities = 237/446 (53%), Positives = 300/446 (67%), Gaps = 22/446 (4%) Query: 1 VDAQHPDTVMVSSLNAWWPDEVIFRSTNGGATWSRIWDWGFYPERTYKFAMDITAAPWLN 60 VD Q P TVM ++ ++WWPD IFRST+ GATW++ WD+ YP R ++ MD++++PWL Sbjct: 336 VDRQKPGTVMATAYSSWWPDTQIFRSTDSGATWTKAWDYSSYPNRANRYTMDVSSSPWLT 395 Query: 61 HGITNSTSLDPSPKLGWMMGDLEIDPFNSDRMMYGTGATIYRTNNLTSWDSGGKVNIAVM 120 G N + + +PKLGWM LEIDPFNS+RMMYGTGAT+Y T NLT+WDSGG+ I M Sbjct: 396 WG-ANPSPPEQAPKLGWMTESLEIDPFNSNRMMYGTGATLYGTENLTNWDSGGQFTIKPM 454 Query: 121 AKGVEETAVLGLISPPSGTAHLITALGDVSGFRYEDLTQAPVKFQTSPSWATTTGIDYAE 180 +G+EETAVL L SPPSG A L++ALGD+ GFR+ DLT+ P TSP++ TTT +D+AE Sbjct: 455 VQGLEETAVLDLASPPSG-APLLSALGDIGGFRHTDLTKVPSMMFTSPNFTTTTSLDFAE 513 Query: 181 LNPAYVVRVGGADKEKTPSMKSIGISNDGGVNWYMPNSEPSNGTKTTAGQGQVAVSASGN 240 NP VVR G D S I S D G NW+ ++PS +AG G VA ++ G+ Sbjct: 514 TNPNTVVRTGNLD-----SGPHIAFSTDNGANWFA-GTDPSG---VSAG-GTVAAASDGS 563 Query: 241 SILWSTSDIGVYYSKTTGNSWNVSSGVPAGAKVASDRVNPNKFYAFYAGTFYIXXXXXXX 300 +WS GV Y+ G+SW+ SSG+PAGA V SDRV+ FY F +G FY+ Sbjct: 564 RFVWSPDGTGVQYTTGFGSSWSASSGIPAGAIVESDRVDAKTFYGFKSGKFYVSKDGGAT 623 Query: 301 XXXXXXXXXXXNNVGGLQPNQAQISMKAMPGIEGDIWFAGGNAVEGKYGLWHSTDSGTSF 360 GL P+ + KA+PG++GD+W AGG A +G YGLWHSTD GTS+ Sbjct: 624 FTASAAS--------GL-PSGDSVRFKALPGVKGDVWLAGG-ATDGAYGLWHSTDGGTSW 673 Query: 361 TKLTNVEEADLIGFGMAAPGQNYMALYTVAKIDGVRGVFRSDDAGSSWVRINDDAHQYAK 420 TKL+NV++AD IGFG AA G +Y LYT AKI GVRGVFRS D G+SW RINDDAHQ+A Sbjct: 674 TKLSNVDQADAIGFGKAATGASYQTLYTSAKIGGVRGVFRSTDKGASWTRINDDAHQWAY 733 Query: 421 INMAITGDPRVYGRVYLGTNGRGTLY 446 AITGDPRVYGRVY+ TNGRG +Y Sbjct: 734 TGAAITGDPRVYGRVYVSTNGRGVIY 759 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.129 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,689,818,847 Number of extensions: 196691238 Number of successful extensions: 396361 Number of sequences better than 1.0e-05: 236 Number of HSP's gapped: 395797 Number of HSP's successfully gapped: 303 Length of query: 796 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 652 Effective length of database: 1,704,070,043 Effective search space: 1111053668036 Effective search space used: 1111053668036 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 136 (57.0 bits)