BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0027.p (207 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P96692.2 YDFN_BACSU Putative NAD(P)H nitroreductase ydfN EC=1.-.-.- 249 8e-65 A7Z110.1 A7Z110_BACA2 YdfN 248 2e-64 B1IH62.1 B1IH62_CLOBK Nitroreductase family protein 247 5e-64 A7FSB1.1 A7FSB1_CLOB1 Nitroreductase family protein 247 5e-64 A5I054.1 A5I054_CLOBH Nitroreductase family protein 247 5e-64 >P96692.2 YDFN_BACSU Putative NAD(P)H nitroreductase ydfN EC=1.-.-.- Length = 206 Score = 249 bits (637), Expect = 8e-65, Method: Compositional matrix adjust. Identities = 119/205 (58%), Positives = 152/205 (74%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M+ F+ LV RRSA+NF+ G I + +L EMF L LAPSA+NLQHT Y V D+D+KEK Sbjct: 1 MAEFTHLVNERRSASNFLSGHPITKEDLNEMFELVALAPSAFNLQHTKYVTVLDQDVKEK 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +++AA GQYK+ ++SAV++VLGDK AY QA++IY GLK+LG +++ +D + D Y Sbjct: 61 LKQAANGQYKVVSSSAVLLVLGDKQAYQQAADIYEGLKVLGILNKQEYDHMVQDTVSFYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 + F+ DEAIRNASLSAM FML AK+KGWDTCPMIGFD +AVK LN+ D VM+I Sbjct: 121 NRGEQFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKRILNIDDQFEVVMMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 TIGK+ RPRGYRKPVNEFVE+ Sbjct: 181 TIGKEKTESRRPRGYRKPVNEFVEY 205 >A7Z110.1 A7Z110_BACA2 YdfN Length = 206 Score = 248 bits (633), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 120/205 (58%), Positives = 150/205 (73%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 M+ F +LV RRSA+NF+ I + EL EMF L LAPSA+NLQHT Y V D D+KEK Sbjct: 1 MAEFVQLVNERRSASNFLPEHPITRDELNEMFELVALAPSAFNLQHTKYVTVLDPDVKEK 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +++AA GQYK+ ++SAV++VLGDK AY QA++IY GLKMLG +++ +D + D Y Sbjct: 61 LKQAANGQYKVFSSSAVLLVLGDKQAYQQAADIYEGLKMLGVLNKQEYDHMVQDTVSFYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 + F+ DEAIRNASLSAM FML AK+KGWDTCPMIGFD +AVK LN+ D VM+I Sbjct: 121 NRGEPFKRDEAIRNASLSAMMFMLSAKEKGWDTCPMIGFDAEAVKQILNIDDQFEVVMMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 TIGK+ RPRGYRKPVNEFVE+ Sbjct: 181 TIGKEKIESRRPRGYRKPVNEFVEY 205 >B1IH62.1 B1IH62_CLOBK Nitroreductase family protein Length = 209 Score = 247 bits (631), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 116/205 (56%), Positives = 151/205 (73%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 MS ++++ RRSANNF+EG+KIP + E+F L +LAPS +N+QH+HY VV+DE KE+ Sbjct: 1 MSELKKIIEERRSANNFIEGVKIPDKDFTEIFELLKLAPSCFNIQHSHYLVVTDEGKKEQ 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +R+AA+ QYK+HTASAVI+VLGDK AY + IYSG+ LG MS+ +D TI DI+ Y Sbjct: 61 LRKAAFNQYKVHTASAVILVLGDKLAYKNSENIYSGMLNLGIMSKLDYDNTIRDINNLYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 G + FQ EAIRN SLSAM FMLIAKDKGWDTCPMIGF+ V+ ++ +N ++I Sbjct: 121 GRGEDFQRSEAIRNTSLSAMMFMLIAKDKGWDTCPMIGFNQDEVRQIFDIPENYEIALMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 T+GK++ K R RGYRKPV EFV F Sbjct: 181 TMGKEDSSKRRMRGYRKPVGEFVSF 205 >A7FSB1.1 A7FSB1_CLOB1 Nitroreductase family protein Length = 209 Score = 247 bits (631), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 116/205 (56%), Positives = 151/205 (73%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 MS ++++ RRSANNF+EG+KIP + E+F L +LAPS +N+QH+HY VV+DE KE+ Sbjct: 1 MSELKKIIEERRSANNFIEGVKIPDKDFTEIFELLKLAPSCFNIQHSHYLVVTDEGKKEQ 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +R+AA+ QYK+HTASAVI+VLGDK AY + IYSG+ LG MS+ +D TI DI+ Y Sbjct: 61 LRKAAFNQYKVHTASAVILVLGDKLAYKNSENIYSGMLNLGIMSKLDYDNTIRDINNLYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 G + FQ EAIRN SLSAM FMLIAKDKGWDTCPMIGF+ V+ ++ +N ++I Sbjct: 121 GRGEDFQRSEAIRNTSLSAMMFMLIAKDKGWDTCPMIGFNQDEVRQIFDIPENYEIALMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 T+GK++ K R RGYRKPV EFV F Sbjct: 181 TMGKEDSSKRRMRGYRKPVGEFVSF 205 >A5I054.1 A5I054_CLOBH Nitroreductase family protein Length = 209 Score = 247 bits (631), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 116/205 (56%), Positives = 151/205 (73%), Gaps = 1/205 (0%) Query: 3 MSTFSELVQARRSANNFVEGIKIPQSELEEMFSLTRLAPSAYNLQHTHYKVVSDEDLKEK 62 MS ++++ RRSANNF+EG+KIP + E+F L +LAPS +N+QH+HY VV+DE KE+ Sbjct: 1 MSELKKIIEERRSANNFIEGVKIPDKDFTEIFELLKLAPSCFNIQHSHYLVVTDEGKKEQ 60 Query: 63 IREAAYGQYKIHTASAVIVVLGDKNAYLQASEIYSGLKMLGAMSEDAFDQTIADIHGAYS 122 +R+AA+ QYK+HTASAVI+VLGDK AY + IYSG+ LG MS+ +D TI DI+ Y Sbjct: 61 LRKAAFNQYKVHTASAVILVLGDKLAYKNSENIYSGMLNLGIMSKLDYDNTIRDINNLYE 120 Query: 123 GN-DAFQHDEAIRNASLSAMQFMLIAKDKGWDTCPMIGFDVQAVKAALNLSDNMVPVMLI 181 G + FQ EAIRN SLSAM FMLIAKDKGWDTCPMIGF+ V+ ++ +N ++I Sbjct: 121 GRGEDFQRSEAIRNTSLSAMMFMLIAKDKGWDTCPMIGFNQDEVRQIFDIPENYEIALMI 180 Query: 182 TIGKDNKHKIRPRGYRKPVNEFVEF 206 T+GK++ K R RGYRKPV EFV F Sbjct: 181 TMGKEDSSKRRMRGYRKPVGEFVSF 205 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,118,685,846 Number of extensions: 41659492 Number of successful extensions: 108658 Number of sequences better than 1.0e-05: 734 Number of HSP's gapped: 108181 Number of HSP's successfully gapped: 734 Length of query: 207 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 78 Effective length of database: 1,845,385,268 Effective search space: 143940050904 Effective search space used: 143940050904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)