BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0021.p (209 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K9D7.1 B3K9D7_9BACI Lipolytic protein G-D-S-L family 284 3e-75 B3KIU5.1 B3KIU5_9BACI Lipolytic protein G-D-S-L family 264 4e-69 A9BFK4.1 A9BFK4_PETMO Lipolytic protein G-D-S-L family 255 2e-66 Q3XYR5.1 Q3XYR5_ENTFC Lipolytic enzyme, G-D-S-L 210 8e-53 B8DCI0.1 B8DCI0_LISMH Lipase/acylhydrolase family protein 208 3e-52 >B3K9D7.1 B3K9D7_9BACI Lipolytic protein G-D-S-L family Length = 211 Score = 284 bits (727), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 134/205 (65%), Positives = 160/205 (78%) Query: 3 FQENDIILFQGDSITDWGRNYEDPSSLGVGYALMVAARLGLLYPEKKLTFINRGISGNRA 62 Q+ D++LFQGDSITD GR+ SLG GY L++ + G+ +PE +TF+NRGISGNR Sbjct: 4 LQQGDVVLFQGDSITDCGRDRNFSGSLGNGYPLLIDSLFGMKFPELNVTFLNRGISGNRV 63 Query: 63 ADLQQRWDRDCLELKPTVVSIYIGINDTWRRFDSGEETTAAQFEASYRDIIERTLRATNA 122 DL RW DCL+LKP VSIYIGINDTWRR+DSG+ T+ ++A YR +IERT A +A Sbjct: 64 VDLNNRWQEDCLDLKPDWVSIYIGINDTWRRYDSGDPTSTEDYKAGYRKLIERTKEALDA 123 Query: 123 KLILVEPFVLPVPEDRKGWRQDLDPKIHVVRELAREYGALLVPLDGLFAAASMKADSAFW 182 KLILVEPFVLP PEDR+ WR+DLDPKI VRELA EYGALLVPLDGLFAAAS++ ++AFW Sbjct: 124 KLILVEPFVLPHPEDRRAWREDLDPKITAVRELAVEYGALLVPLDGLFAAASVRREAAFW 183 Query: 183 APDGVHPSPAGHALITDAWLKAAGA 207 APDGVHPSPAGH LI AWL+ G Sbjct: 184 APDGVHPSPAGHGLIAKAWLETMGV 208 >B3KIU5.1 B3KIU5_9BACI Lipolytic protein G-D-S-L family Length = 212 Score = 264 bits (674), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 122/202 (60%), Positives = 155/202 (76%) Query: 1 MEFQENDIILFQGDSITDWGRNYEDPSSLGVGYALMVAARLGLLYPEKKLTFINRGISGN 60 + ++N ++LFQGDSITD GRNYE+ SLG GYALM AARLG+ P K+L F+NRGISGN Sbjct: 2 VHIEQNALVLFQGDSITDCGRNYEESDSLGHGYALMAAARLGMELPLKRLRFVNRGISGN 61 Query: 61 RAADLQQRWDRDCLELKPTVVSIYIGINDTWRRFDSGEETTAAQFEASYRDIIERTLRAT 120 R DL+ RW+ DC++L PT VSI +GIN+T RRF+ GE T+ + YR+++ +T T Sbjct: 62 RVVDLRGRWEEDCIDLDPTWVSILVGINETGRRFNRGETTSVEAYYEGYRELLIQTRELT 121 Query: 121 NAKLILVEPFVLPVPEDRKGWRQDLDPKIHVVRELAREYGALLVPLDGLFAAASMKADSA 180 A IL+EPF+LPV E +K WR+DLDPKI VREL+RE+ L VPLDG+FAAAS K + + Sbjct: 122 GASFILMEPFLLPVTEAKKEWREDLDPKIQAVRELSREFETLYVPLDGIFAAASAKIEMS 181 Query: 181 FWAPDGVHPSPAGHALITDAWL 202 +WAPDGVHPSPAGHAL+ DAWL Sbjct: 182 YWAPDGVHPSPAGHALMADAWL 203 >A9BFK4.1 A9BFK4_PETMO Lipolytic protein G-D-S-L family Length = 208 Score = 255 bits (651), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 114/208 (54%), Positives = 154/208 (74%) Query: 1 MEFQENDIILFQGDSITDWGRNYEDPSSLGVGYALMVAARLGLLYPEKKLTFINRGISGN 60 M ++N I+LFQGDS+TD GR+Y + + LG+GY ++ A+ L YP K + F N+G+SGN Sbjct: 1 MLIEDNAIVLFQGDSVTDAGRDYNNDADLGLGYPMITASWLSAAYPAKNIRFTNKGVSGN 60 Query: 61 RAADLQQRWDRDCLELKPTVVSIYIGINDTWRRFDSGEETTAAQFEASYRDIIERTLRAT 120 R DL++RW RDC+ LKPT VSI IGIND WRR+DS + T+ +FE+ YR I++ Sbjct: 61 RVKDLKERWMRDCIALKPTWVSILIGINDCWRRYDSDDPTSVEKFESDYRYILQEVKTQL 120 Query: 121 NAKLILVEPFVLPVPEDRKGWRQDLDPKIHVVRELAREYGALLVPLDGLFAAASMKADSA 180 NA LI+ EPFVLPV +++ WR+DLDPKIH VR+LARE+ A+ +PLDG+FA A+ + + Sbjct: 121 NANLIICEPFVLPVTKEQAKWREDLDPKIHAVRKLAREFNAIFLPLDGIFAQAATQKNPR 180 Query: 181 FWAPDGVHPSPAGHALITDAWLKAAGAI 208 FW PDGVHPSPAGHALI +WL+A A+ Sbjct: 181 FWLPDGVHPSPAGHALIAQSWLRAVEAL 208 >Q3XYR5.1 Q3XYR5_ENTFC Lipolytic enzyme, G-D-S-L Length = 224 Score = 210 bits (534), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 104/205 (50%), Positives = 134/205 (65%), Gaps = 5/205 (2%) Query: 9 ILFQGDSITDWGRNYEDPSSLGVGYALMVAARLGLLYPEKKLTFINRGISGNRAADLQQR 68 ILF GDSITD GR+ + + LG GY L++A +L YP K +NRGI G++ ADLQ R Sbjct: 5 ILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTMKFECMNRGIGGDKIADLQVR 64 Query: 69 WDRDCLELKPTVVSIYIGINDTWRRFDS----GEETTAAQFEASYRDIIERTLRATNAKL 124 W DCL L+P VSI IGINDTW G E +A QFE+ YR ++ A + Sbjct: 65 WQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSAQQFESVYRKLLNELQTAEICNI 124 Query: 125 ILVEPFVLPVPEDRKGWRQDLDPKIHVVRELAREYGALLVPLDGLFAAASMKAD-SAFWA 183 IL+EPFVLP+ +DR GWR+DLDPKI +VR+LA+EYGALLVPLDG+F A + S + Sbjct: 125 ILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYGALLVPLDGIFNALGIAYGFSKYTR 184 Query: 184 PDGVHPSPAGHALITDAWLKAAGAI 208 DGVHP+ GH +I +W+ A A+ Sbjct: 185 EDGVHPTIGGHEIIAQSWIDAIAAV 209 >B8DCI0.1 B8DCI0_LISMH Lipase/acylhydrolase family protein Length = 197 Score = 208 bits (529), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 104/195 (53%), Positives = 132/195 (67%), Gaps = 4/195 (2%) Query: 9 ILFQGDSITDWGRNYEDPSSLGVGYALMVAARLGLLYPEKKLTFINRGISGNRAADLQQR 68 +LF GDS+TD GR++E+ LG GY M+A +L ++ +T INRG+S NR ADL +R Sbjct: 4 LLFLGDSVTDAGRDFENDRELGHGYVKMIAEQL----EKEDVTVINRGVSANRVADLHRR 59 Query: 69 WDRDCLELKPTVVSIYIGINDTWRRFDSGEETTAAQFEASYRDIIERTLRATNAKLILVE 128 + D + L+P VV+I IGINDTW F E+T+ F+ YR I+ R TNA+LIL+E Sbjct: 60 IEADAISLQPDVVTIMIGINDTWFSFSRWEDTSVTAFKEVYRVILNRIKTETNAELILME 119 Query: 129 PFVLPVPEDRKGWRQDLDPKIHVVRELAREYGALLVPLDGLFAAASMKADSAFWAPDGVH 188 PFVLP PEDRK WR DLDPKI VRELA E+GA L+PLDGL A ++K F A DGVH Sbjct: 120 PFVLPYPEDRKEWRGDLDPKIGAVRELAAEFGATLIPLDGLMNALAIKHGPTFLAEDGVH 179 Query: 189 PSPAGHALITDAWLK 203 P+ AGH I WL+ Sbjct: 180 PTKAGHEAIASTWLE 194 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,273,698,150 Number of extensions: 49980570 Number of successful extensions: 129426 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 129380 Number of HSP's successfully gapped: 98 Length of query: 209 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 79 Effective length of database: 1,835,964,253 Effective search space: 145041175987 Effective search space used: 145041175987 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)