BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0574.0009.p (311 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KAZ2.1 B3KAZ2_9BACI Glyoxalase/bleomycin resistance protein/di... 310 8e-83 Q65MB8.1 Q65MB8_BACLD YfiE 296 2e-78 P54721.2 YFIE_BACSU Uncharacterized protein yfiE 286 2e-75 A7Z2K1.1 A7Z2K1_BACA2 YfiE 281 7e-74 B1YHY5.1 B1YHY5_EXIS2 Glyoxalase/bleomycin resistance protein/di... 267 7e-70 >B3KAZ2.1 B3KAZ2_9BACI Glyoxalase/bleomycin resistance protein/dioxygenase Length = 284 Score = 310 bits (795), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 155/282 (54%), Positives = 199/282 (70%), Gaps = 1/282 (0%) Query: 28 AVLPQSLEIGLVQLRVSDLERSLAFYQNVVGLKVLRQNGREVEMTADGQHVLLVLREIKN 87 A++ S ++G V LR+ +L +S+ FY VVGLK+LRQ+GR E+TADG+ LL++ E+ + Sbjct: 3 AIIHPSTKLGPVSLRIRNLAQSVRFYTEVVGLKMLRQHGRVAELTADGKQPLLIIEEVSD 62 Query: 88 ARVLRRNSAAGLYHFAILVPDRPSLGLVLRNLIDSGIHIGQGDHLVSEALYIQDPDNNGI 147 + + AGLYHFAILVPDR SLGL L NLI I +GQGDHLVSEALYI DPD NGI Sbjct: 63 L-IRPQTRTAGLYHFAILVPDRESLGLALANLIAHNIPVGQGDHLVSEALYINDPDGNGI 121 Query: 148 ELYRDRPRDTWKHEANGNVVMTTDPVDVDGLLAASEGLSWTGLPAGTVIGHVHFHVGDIG 207 E+Y DRPRDTWK +A G VM TDPVDV+GLLA S+ W GLP GTVIGHVHFHV ++ Sbjct: 122 EIYADRPRDTWKRDAKGEYVMATDPVDVEGLLALSKEKEWLGLPEGTVIGHVHFHVSNLH 181 Query: 208 EAKKFYVDTLGFELTAHYGDAAMFISAGGYHHHMGLNIWXXXXXXXXXXXXXXIDYFTLL 267 +A+ FY D LGFELTAHYG AA+FISAGGYHHH+GLN+W ++ +T+ Sbjct: 182 DARAFYCDVLGFELTAHYGGAALFISAGGYHHHIGLNVWAGIGVPNTPPNAAGMENYTIE 241 Query: 268 LPSVQERDLAAERVRSAGYRVDEAGGIVTVTDPWNIGIQLLV 309 LPS + + R+R AG +++ G+ V DP +I I+L+V Sbjct: 242 LPSHEALEAVTGRIREAGLHLEQRDGVWAVQDPSDIWIRLVV 283 >Q65MB8.1 Q65MB8_BACLD YfiE Length = 286 Score = 296 bits (757), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 145/277 (52%), Positives = 197/277 (71%), Gaps = 2/277 (0%) Query: 35 EIGLVQLRVSDLERSLAFYQNVVGLKVLRQNGREVEMTADGQHVLLVLREIKNARVLRRN 94 E+G V+L+VS LERS++FY++V+G +VL+Q+G+ E T DG++ LL++ EI NA VL Sbjct: 10 ELGYVKLKVSRLERSVSFYRDVIGFQVLKQDGKTAEFTVDGKNPLLIIEEIPNAVVLPER 69 Query: 95 SAAGLYHFAILVPDRPSLGLVLRNLIDSGIHIGQGDHLVSEALYIQDPDNNGIELYRDRP 154 SAAGLYHFAIL+P+R LG+ +++LI +GI +GQGDHLVSEA Y+ DPD NGIE+YRDRP Sbjct: 70 SAAGLYHFAILLPNRKQLGMAVKHLIRAGIELGQGDHLVSEAFYLSDPDQNGIEIYRDRP 129 Query: 155 RDTWKHEANGNVVMTTDPVDVDGLLAASEGLSWTGLPAGTVIGHVHFHVGDIGEAKKFYV 214 R+ WK + GN VM T+PVDV GLL ++G W GLP T IGHVHFHV D+ +AK+FY Sbjct: 130 REGWKRDEQGNYVMGTEPVDVQGLLEEADGHEWQGLPPETKIGHVHFHVRDLKQAKEFYH 189 Query: 215 DTLGFELTAHYGD-AAMFISAGGYHHHMGLNIWXXXXXXXXXXXXXXIDYFTLLLPSVQE 273 LGF++T Y +A+F+SAGGYHHH+GLNIW + Y+T+LLP+ E Sbjct: 190 SVLGFDITGDYQHMSALFVSAGGYHHHIGLNIWAGQHAPFAPLDAAGMAYYTVLLPNRAE 249 Query: 274 RDLAAERVRSAGYRVDEAGGIVTVTDPWN-IGIQLLV 309 D A++++S+GY V+ V DP + I I+L V Sbjct: 250 LDRIAKKLKSSGYAVEHEENAWFVKDPASGIDIRLTV 286 >P54721.2 YFIE_BACSU Uncharacterized protein yfiE Length = 285 Score = 286 bits (731), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 139/270 (51%), Positives = 179/270 (66%), Gaps = 1/270 (0%) Query: 32 QSLEIGLVQLRVSDLERSLAFYQNVVGLKVLRQNGREVEMTADGQHVLLVLREIKNARVL 91 + IG +L + LERSL FY NV+G +VL++ R+ E+TADG+ VLL+L E +A VL Sbjct: 6 EDTHIGYAKLTIRSLERSLQFYCNVIGFQVLKKTDRQAELTADGKRVLLILEENPSAVVL 65 Query: 92 RRNSAAGLYHFAILVPDRPSLGLVLRNLIDSGIHIGQGDHLVSEALYIQDPDNNGIELYR 151 S GLYHFAIL+PDR LG+ L LI+ GI IG GDH VSEALY+ DPD NGIE+Y Sbjct: 66 PERSVTGLYHFAILLPDRKELGIALARLIEHGIAIGHGDHAVSEALYLSDPDGNGIEMYA 125 Query: 152 DRPRDTWKHEANGNVVMTTDPVDVDGLLAASEGLSWTGLPAGTVIGHVHFHVGDIGEAKK 211 DRPR TW+ + GN VMTT VD++GLL + T LP T+IGH+H HV D+ EAK Sbjct: 126 DRPRSTWQRDREGNYVMTTTAVDIEGLLEEAGDERKTSLPNDTIIGHIHLHVSDLKEAKA 185 Query: 212 FYVDTLGFELTAHY-GDAAMFISAGGYHHHMGLNIWXXXXXXXXXXXXXXIDYFTLLLPS 270 FY D LGF++ +Y G +A+F+SAGGYHHH+GLNIW +DY+T++LP Sbjct: 186 FYTDVLGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGRNAPPKPTNASGLDYYTVVLPH 245 Query: 271 VQERDLAAERVRSAGYRVDEAGGIVTVTDP 300 +E DL A RV+ AGY ++E V DP Sbjct: 246 QEELDLVANRVKHAGYSIEETENSFRVKDP 275 >A7Z2K1.1 A7Z2K1_BACA2 YfiE Length = 285 Score = 281 bits (718), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 139/270 (51%), Positives = 179/270 (66%), Gaps = 1/270 (0%) Query: 32 QSLEIGLVQLRVSDLERSLAFYQNVVGLKVLRQNGREVEMTADGQHVLLVLREIKNARVL 91 Q IG V+L + ++ERSL FY+NV+GL+V+ Q R ++ADG+ VLLVL E A VL Sbjct: 6 QDTAIGYVKLTIKNMERSLGFYRNVIGLQVISQTDRSARLSADGKRVLLVLEENPGAVVL 65 Query: 92 RRNSAAGLYHFAILVPDRPSLGLVLRNLIDSGIHIGQGDHLVSEALYIQDPDNNGIELYR 151 S GLYHFAIL+PDR LG+ L LI++GI +GQGDH VSEALY+ DPD NGIE+Y Sbjct: 66 PERSVTGLYHFAILLPDRKELGIALARLIENGIALGQGDHAVSEALYLSDPDGNGIEIYA 125 Query: 152 DRPRDTWKHEANGNVVMTTDPVDVDGLLAASEGLSWTGLPAGTVIGHVHFHVGDIGEAKK 211 DRPR TW+ +A GN VM T PVDVDGLL + + LP GTVIGH+H HV D+ EAK Sbjct: 126 DRPRSTWRRDAEGNYVMQTAPVDVDGLLEEAGAERKSRLPEGTVIGHIHLHVTDLQEAKA 185 Query: 212 FYVDTLGFELTAHY-GDAAMFISAGGYHHHMGLNIWXXXXXXXXXXXXXXIDYFTLLLPS 270 FY D LGF++ +Y G +A+F+SAGGYHHH+GLNIW +DY+T+ LP Sbjct: 186 FYTDILGFDIVGNYAGMSALFVSAGGYHHHIGLNIWAGKHAPPKPGNASGLDYYTIALPH 245 Query: 271 VQERDLAAERVRSAGYRVDEAGGIVTVTDP 300 +E + + AG+ ++E V V DP Sbjct: 246 QKELSRVTKALTDAGFEIEEKEEGVFVKDP 275 >B1YHY5.1 B1YHY5_EXIS2 Glyoxalase/bleomycin resistance protein/dioxygenase Length = 284 Score = 267 bits (683), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 133/279 (47%), Positives = 182/279 (65%), Gaps = 2/279 (0%) Query: 33 SLEIGLVQLRVSDLERSLAFYQNVVGLKVLRQNGREVEMTADGQHVLLVLREIKNARVLR 92 ++ +G V LR+++LE+S+AFY++ +GL VLRQ+ + + A L++L + A+ L Sbjct: 6 NITLGPVSLRITNLEQSIAFYRDALGLTVLRQDEQTATLGAKTTP-LVILEQHSTAKQLP 64 Query: 93 RNSAAGLYHFAILVPDRPSLGLVLRNLIDSGIHIGQGDHLVSEALYIQDPDNNGIELYRD 152 +NS AGLYHFAIL+P+R LG V+RNLI GI +GQGDHLVSEA Y+ DPD NGIE+Y D Sbjct: 65 KNSVAGLYHFAILLPNRKELGFVIRNLIAQGIELGQGDHLVSEAFYLSDPDGNGIEIYAD 124 Query: 153 RPRDTWKHEANGNVVMTTDPVDVDGLLAASEGLSWTGLPAGTVIGHVHFHVGDIGEAKKF 212 RPRDTW +EANG+V MTTDPVD +L + W G+PA TV+GHVHFHV + A+KF Sbjct: 125 RPRDTWTYEANGDVKMTTDPVDWQSMLVEAGEEDWYGMPAETVMGHVHFHVKSLEAARKF 184 Query: 213 YVDTLGFELTAHYGDA-AMFISAGGYHHHMGLNIWXXXXXXXXXXXXXXIDYFTLLLPSV 271 YV+TLGF++ A A+F++AGGYHHH+G N+W + Y+TL+ P Sbjct: 185 YVETLGFDVAADASRMRALFLAAGGYHHHIGTNVWSGVDAPKTPADAVGLKYWTLIYPDA 244 Query: 272 QERDLAAERVRSAGYRVDEAGGIVTVTDPWNIGIQLLVK 310 R+ A + + Y V+ V VTD I +LL K Sbjct: 245 ASRETAVAALEQSPYEVNRQEDAVYVTDDAGITARLLSK 283 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,818,552,494 Number of extensions: 74881392 Number of successful extensions: 194260 Number of sequences better than 1.0e-05: 417 Number of HSP's gapped: 193009 Number of HSP's successfully gapped: 455 Length of query: 311 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 176 Effective length of database: 1,788,859,178 Effective search space: 314839215328 Effective search space used: 314839215328 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)