BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0571.0018.p (421 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z942.1 C0Z942_BREBN Probable multidrug resistance protein 310 2e-82 Q65F99.1 Q65F99_BACLD Multidrug-efflux transporter 295 6e-78 A7Z8G2.1 A7Z8G2_BACA2 Multidrug resistance protein 295 6e-78 B3KGD3.1 B3KGD3_9BACI Major facilitator superfamily MFS_1 255 7e-66 B7HXI2.1 B7HXI2_BACC7 Multidrug resistance protein 244 1e-62 >C0Z942.1 C0Z942_BREBN Probable multidrug resistance protein Length = 401 Score = 310 bits (794), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 164/386 (42%), Positives = 232/386 (60%), Gaps = 5/386 (1%) Query: 32 NNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPITG 91 N++R + LMIN+FI MLG+GLI PILP+ + EFGAGG++ G+LVAA+G+TQFL SPI G Sbjct: 8 NSERPILFLMINMFIAMLGIGLIIPILPEFLKEFGAGGETAGYLVAAFGVTQFLFSPIAG 67 Query: 92 QLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVADI 151 + SD+YGRK I+ G W+LY SRL+ G+ AA +IP+M+A VADI Sbjct: 68 EWSDKYGRKIMIVMGLVLFTISNLVFALAEQTWVLYLSRLIGGIGAAAMIPSMLAYVADI 127 Query: 152 TTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCLPE 211 TT ++R KG +L AAMS R+P Y SIF L E Sbjct: 128 TTEDKRGKGLGMLGAAMSLGFVIGPGIGGFLAELGLRMPFYISAAVGAVATLGSIFFLSE 187 Query: 212 SISKEQMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVTNR 271 S+ KE+ A+R +E+IF Q KS +S Y ++ LV MTFGLANFE +F L+V + Sbjct: 188 SLPKEKQLASRNAKDKQENIFLQLGKSFQSSYFIMLVLVFTMTFGLANFEVIFPLFVDAK 247 Query: 272 FQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXXXX 331 F +TP++ISII+T GA+ G +QA ++ K+I FGE+K+I ++ Sbjct: 248 FAYTPRDISIIITVGALAGTIVQAALIGKLITRFGEKKLIN-----VTFFLSAVSMVMML 302 Query: 332 XXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPTLA 391 F +++RPA+NTL+SKRAG EQG+ AGMNNAYMS+GNI GP +A Sbjct: 303 LSGNFWYMLIMTVLFFTLTSIMRPAINTLISKRAGDEQGFVAGMNNAYMSLGNIFGPAVA 362 Query: 392 GFLFDINMFAPFIAGCCIIFITFLMT 417 G L+ +++ AP++ G I+ ++ ++ Sbjct: 363 GTLYGVHLNAPYLFGAIILVLSLFLS 388 >Q65F99.1 Q65F99_BACLD Multidrug-efflux transporter Length = 398 Score = 295 bits (755), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 151/383 (39%), Positives = 226/383 (59%), Gaps = 5/383 (1%) Query: 37 LAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPITGQLSDR 96 L +LM+N+FIVMLG+GLI P++P I EFGA G ++G L+AA G+TQ L SP+ G+++D+ Sbjct: 7 LYMLMLNVFIVMLGIGLIIPLMPTFIEEFGASGSTLGLLIAASGITQLLFSPVAGEMTDK 66 Query: 97 YGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVADITTTEE 156 YGR+ II G + +W+L+ SRLL G AA ++P M A +ADIT+ ++ Sbjct: 67 YGRRKMIILGIGAFAVSQLLFALASQMWLLFVSRLLGGAGAAFLVPAMFAYIADITSEKD 126 Query: 157 RAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCLPESISKE 216 R+KG L++AAMS P Y S+ LPE++S+E Sbjct: 127 RSKGMGLISAAMSLGFVIGPGAGGYLAAFGLTFPFYVSAGLAGLATVLSLLVLPETLSQE 186 Query: 217 QMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVTNRFQFTP 276 +M R V +E + +Q A++LRS Y LF LV ++ FG+ NFE VF LYV ++ FTP Sbjct: 187 KMLEKRRSVQKREPLAKQMARALRSPYAFLFILVFILNFGIMNFEAVFSLYVDHKHGFTP 246 Query: 277 QNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXXXXXXXXX 336 +I+ ++TA ++IGV +QA+ + + FGE+K++ +L+ Sbjct: 247 GDIAFVITAASLIGVFVQAVALGMLTNRFGEKKLMNMTLI-----GSAGALAVCSIAGSY 301 Query: 337 XXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPTLAGFLFD 396 F ++LRPA+NTL+SK AG EQG+AAGMNNA+MS+ NI GPT+AG LFD Sbjct: 302 WLVFGATIVFFMLTSILRPAINTLISKMAGDEQGFAAGMNNAFMSLANIVGPTVAGLLFD 361 Query: 397 INMFAPFIAGCCIIFITFLMTIK 419 +N+ P+I G ++ ++FL + Sbjct: 362 VNVEIPYIFGVVVLVLSFLAAVS 384 >A7Z8G2.1 A7Z8G2_BACA2 Multidrug resistance protein Length = 398 Score = 295 bits (755), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 157/381 (41%), Positives = 227/381 (59%), Gaps = 9/381 (2%) Query: 37 LAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPITGQLSDR 96 L+ILM+N+FI MLG+GLI PI+P ITEFGA G ++G LVAA GLTQ L +PI G+++D+ Sbjct: 7 LSILMLNMFIAMLGIGLIIPIMPTYITEFGATGSTMGLLVAAAGLTQLLFAPIAGEITDK 66 Query: 97 YGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVADITTTEE 156 YGR+ II G +LW L+ SRLL G+ AA ++P+M A +ADITT EE Sbjct: 67 YGRRKLIIFGIAAFAVSQLIFAFAGSLWQLFASRLLGGMGAAFLMPSMFAYIADITTEEE 126 Query: 157 RAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCLPESISKE 216 R KG L +AAM+ VP S F LPE++ +E Sbjct: 127 RGKGMGLFSAAMTLGVVIGPGVGGYLVHYGMSVPFIVSASLACFSSVLSFFFLPETLGRE 186 Query: 217 QMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVTNRFQFTP 276 + ARAK +E +F+Q +++L+S + + LV V+ FG+ NFE+VFGLYV ++ F Sbjct: 187 KQLEARAKTEKREHLFKQMSRALKSPFAFMLILVFVLNFGIMNFESVFGLYVDHKHGFKA 246 Query: 277 QNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXXXXXXXXX 336 +I+ I+TA +IGV +QA+ V+ +++ FGE++VI +L+ Sbjct: 247 SDIAFIITAAGLIGVFVQAVGVSYLVRRFGEKRVINVTLI-------GAAAGLVWCRFAG 299 Query: 337 XXXXXXXXXXFFAN--AMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPTLAGFL 394 FF ++LRPA+NT+LSK AG +QG+A GMN +++S+ NIAGP+ AGFL Sbjct: 300 SYWAVFAAAIFFLTLTSLLRPAVNTMLSKLAGDQQGFAGGMNTSFISLANIAGPSAAGFL 359 Query: 395 FDINMFAPFIAGCCIIFITFL 415 FD+NM P++ G I+ I+FL Sbjct: 360 FDVNMEFPYMLGAAILTISFL 380 >B3KGD3.1 B3KGD3_9BACI Major facilitator superfamily MFS_1 Length = 401 Score = 255 bits (651), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 142/390 (36%), Positives = 219/390 (56%), Gaps = 8/390 (2%) Query: 30 LTNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPI 89 L NN IL +LM NIF+V G+GL+ PI+P +TE G ++G LVAA+ LTQ L SP+ Sbjct: 5 LRNNGAIL-LLMFNIFLVFTGIGLVIPIMPTYMTELHISGSTVGMLVAAFSLTQLLFSPL 63 Query: 90 TGQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVA 149 G+LSD GRK II+G + +L+ +R+L G+ AALI+P +MA A Sbjct: 64 AGRLSDIMGRKKIIISGMIVFAISEWLFGAASTPTLLFAARMLGGIGAALIMPAVMAYTA 123 Query: 150 DITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCL 209 D+T+ EERA+G + AA++ RVP Y ++ L Sbjct: 124 DVTSNEERARGMGFINAAITTGFIIGPGIGGFIAEFGIRVPFYAAAVAGGIAALVTLLLL 183 Query: 210 PESISKEQMNAARAKVAPKESIFEQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYVT 269 PES +K++ + +ES+ Q +S R Y + +V V++FGLAN+ETVFGL+V Sbjct: 184 PESSAKQEAPTVLGR--KQESLAVQLVRSFREPYFLSLIIVFVLSFGLANYETVFGLFVD 241 Query: 270 NRFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXXX 329 ++F FTP++I++I+T G++ G +QA + ++ FGE++VI SLL Sbjct: 242 HKFGFTPKDIAMIITFGSIAGAVVQATIFGWILNRFGEKRVITLSLL-----TAGICILL 296 Query: 330 XXXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGPT 389 F + +LRPA+ T +SK A +QGY AG+N+AY S+GNIAGP Sbjct: 297 TLFVHKYWLIFLVTFLVFLSIDILRPAIGTQMSKLAKDQQGYVAGLNSAYTSLGNIAGPL 356 Query: 390 LAGFLFDINMFAPFIAGCCIIFITFLMTIK 419 +AG+LFD+++ P+I+ ++ + F + ++ Sbjct: 357 VAGYLFDLDINFPYISASIVLILCFFLAMR 386 >B7HXI2.1 B7HXI2_BACC7 Multidrug resistance protein Length = 400 Score = 244 bits (622), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 141/375 (37%), Positives = 204/375 (54%), Gaps = 8/375 (2%) Query: 30 LTNNKRILAILMINIFIVMLGVGLITPILPKLITEFGAGGQSIGFLVAAYGLTQFLLSPI 89 + K +L IL+ NIFI LG+GLI P++P + + G G+++G+LVA + + Q + SPI Sbjct: 5 IKEQKMVLVILLSNIFIAFLGIGLIIPVMPSFMNDMGLTGKTMGYLVAVFAMAQLIASPI 64 Query: 90 TGQLSDRYGRKTFIITGXXXXXXXXXXXXXGNALWMLYTSRLLEGVAAALIIPTMMACVA 149 TG+ D YGRK II G G +WMLY +R+L G++AA I+P + A VA Sbjct: 65 TGRWVDLYGRKKMIIIGLFIFGVSELLFGLGTDVWMLYAARVLGGISAAFIMPGVTAYVA 124 Query: 150 DITTTEERAKGNSLLAAAMSXXXXXXXXXXXXXXXXXTRVPLYXXXXXXXXXXXFSIFCL 209 DIT+ +ER K L+AA+S RVP + SIF L Sbjct: 125 DITSIQERPKAMGYLSAAISTGFIIGPGIGGFIAEYGIRVPFFVAAVIAFVACVISIFIL 184 Query: 210 PESISKEQMNAARAKVAPKESIF-EQYAKSLRSKYVMLFALVLVMTFGLANFETVFGLYV 268 E ++KE++ A KES F KSL Y + F +V V+ FGL+ +ETVF L+ Sbjct: 185 KEPLTKEEL--AEISSNTKESSFIGDLKKSLHPMYAIAFIIVFVLAFGLSAYETVFSLFS 242 Query: 269 TNRFQFTPQNISIILTAGAVIGVGMQALVVAKVIKIFGEQKVIKGSLLIISXXXXXXXXX 328 ++F FTP++I+ I+T ++ GV +Q + K++ IFGE+ +I+ L++ Sbjct: 243 DHKFGFTPKDIAAIITISSIFGVVVQVFMFGKLVDIFGEKVLIQICLIV-----GAVLAF 297 Query: 329 XXXXXXXXXXXXXXXXXXFFANAMLRPALNTLLSKRAGTEQGYAAGMNNAYMSVGNIAGP 388 F A +LRPAL T LSK AG EQG+ AGMN+ Y S+GNIAGP Sbjct: 298 VSTVVFNYWIVLLVTCFIFLAFDLLRPALTTFLSKAAGKEQGFVAGMNSTYTSLGNIAGP 357 Query: 389 TLAGFLFDINMFAPF 403 + G LFD+N+ P+ Sbjct: 358 AMGGILFDMNIHYPY 372 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.327 0.140 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,779,043,514 Number of extensions: 64725642 Number of successful extensions: 262707 Number of sequences better than 1.0e-05: 1562 Number of HSP's gapped: 265679 Number of HSP's successfully gapped: 1611 Length of query: 421 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 283 Effective length of database: 1,760,596,133 Effective search space: 498248705639 Effective search space used: 498248705639 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)